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Citations for Becton, Dickinson and Company :
3651 - 3700 of 8884 citations for 7 Chloro 3 methyl 3H 1 2 3 triazolo 4 5 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... HeLa and RAW 264.7 cells were washed by chilled PBS and sorted on the nanoPOTS chips (4 × 12, 1.2 mm diameter per well) using the Influx II cell sorter (BD Biosciences, San Jose, CA) as described previously 14 ...
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bioRxiv - Immunology 2020Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... mice were euthanized and tumors were quickly harvested and fixed for 14-16 hr at 4&C in BD Cytofix/Cytoperm (BD Bioscience, Cat #: 554722) diluted 1:4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... In-between incubation steps, cells were washed (300 x g, 10 min, 4°C) with 40 mL of BD Cell Wash (BD Biosciences, Allschwil, Switzerland). In order to block Fc receptors ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the samples were fixated and permeabilized for 20 min and 60 min at 4 °C in the dark using BD Cytofix/Cytoperm™ solution and BD Perm/Wash™ solution (BD Biosciences), respectively ...
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bioRxiv - Microbiology 2022Quote: ... the individual co-cultures were 10-fold serially diluted with 1X anaerobic phosphate buffer saline (PBS) and plated on Xylose Lysine Tergitol 4 (XLT4) agar (BD Difco, Houston, TX). The plates were incubated aerobically at 37°C for 24 hours followed by plating on XLT4 agar and colony forming units (CFU ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were incubated in the 9-well plates with 100 μL per plate overnight at 4°C: CtBP2 mouse IgG1 (BD Biosciences 612044; RRID:AB_399431), GluA2 mouse IgG2a (Millipore MAB397 ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... overnight and then stimulated with 1 nM N4 in the presence of vehicle or Compound 182 (1 μM) for 4 h at 37°C in complete T cell media supplemented with BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were then stained with fluorophore-conjugated antibodies to cell surface CD8 and intracellular IFNγ ...
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bioRxiv - Immunology 2023Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Genomics 2023Quote: ... and half of the remaining chromatin was rotated overnight at 4°C with 4.5 µg of anti-p53 (BD Biosciences #554293, Lot #9046678). The other half of the remaining chromatin was also rotated overnight as a no-antibody control ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... cells were re-suspended in 150 μl of PBS/5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression ...
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bioRxiv - Cell Biology 2020Quote: ... was prepared the day before the experiment by incubating the wells with 50μl/well of 5 μg/ml of anti-integrin α5 antibodies (BD Biosciences, 555651) in 0.05m Na2CO3 pH 9.6 ...
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bioRxiv - Cell Biology 2020Quote: ... The CSCs were selected as the top 5% cells with the highest GFP intensity (Oct4hi) and then sorted with the FACSAria II cell sorter (BD Biosciences).
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bioRxiv - Genetics 2021Quote: ... and non-neuronal (NeuN−) nuclei were sorted into tubes pre-coated with 5% BSA using a FACSAria flow cytometer (BD Biosciences) equipped with a 100μm nozzle ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples (10,000 events per sample) were analyzed after 5-10 min incubation in the dark on ice on a FACSCalibur flow cytometer (BD Biosciences) in the FL3 channel.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in 1X PBS to remove excess stain with a centrifugation step of 5 min at 2000 ×g and run on a BD FACSCanto cytometer (BD Biosciences). Based on the previous flow cytometry data for establishment of threshold values for gating (Kwon and Smith ...
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bioRxiv - Molecular Biology 2021Quote: ... the top 5% cells expressing the highest levels of CasRx-GFP were sorted using BD FACS ARIA II (BD Bioscience, USA). The CasRx-GFP expressing cells were expanded and screened for CasRx expression using qPCR (primers listed in Supplementary Table S1).
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bioRxiv - Cancer Biology 2019Quote: ... Cells were passaged and cultured under puromycin selection for at least 5 days before flow cytometry analysis using an LSRFortessa X20 instrument (BD Biosciences).
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bioRxiv - Developmental Biology 2019Quote: ... Cells were incubated with TrypLE Express for 5 min at 37 °C to generate single cell suspensions and strained through a 40 μm mesh (BD Biosciences). They were then fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... 25 μL of cell suspension from each well was washed with 100 μL PBS-EDTA and stained with 5 μL of HLA-DR-FITC (clone G46-6, BD BioSciences) or isotype control (clone G155-178 ...
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bioRxiv - Genetics 2019Quote: ... in PBS + 2.5% FBS and viable single cells were sorted and deposited into the MS-5 seeded 96 well plate using the FACSAria II (BD Biosciences). Single cells were cultured for 16-17 days with an addition of 100μl erythro-myeloid differentiation media per well at day 8.
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bioRxiv - Genetics 2021Quote: ... Cells were passed through a cell-strainer cap on a 5 mL polystyrene round-bottom tube (FALCON 352235) and GFP signal was measured on a BD LSR Fortessa (BD Biosciences) at the Flow Cytometry Facility at the University of California ...
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bioRxiv - Genetics 2021Quote: ... The worms were then dissected at the pharynx with a pair of 30G 5/8” needles (PrecisionGlide, BD, Franklin Lakes, NJ). The dissected gonads were fixed with 3% paraformaldehyde (EM Grade ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were implanted subcutaneously in the flank of BALB/c mice using 5 × 106 cells (WEHI-164) with 0.5 ml 29G insulin syringes (MicroFine™+, BD medical). Tumor volume was measured with a caliper and volume was calculated using the formula ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were pelleted at 400xg for 5 minutes and resuspended in 200 µL PBS for immediate analysis with flow cytometry (BD Accuri). Negative/positive nanogel or anti-CD45 signal was established by comparison to unstained cells ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells or MNC from CML patients or HD were incubated with the following antibodies: 5 µL CD45-PerCP (2D1, BD Biosciences), 2.5 µL CD34-FITC (AC136 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were implanted with 5 x 104 MDA-MB-231 breast cancer cells in 50% cell culture medium (FBS free) and 50% Matrigel (BD biosciences) by subcutaneous injection in the left dorsal flank ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were incubated for 25 mins at 37°C 5% CO2 and then fixed and permeabilized with CytoFix/CytoPerm (BD Biosciences) at room temperature for 20 mins ...
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bioRxiv - Microbiology 2021Quote: ... 5-10 Spheroids resuspended in serum-free medium were seeded in an insert that has been coated with Matrigel (BD Biosciences). Lower compartment was added with medium containing 20% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Salivary glands were cleanly dissected from infected mosquitoes placed into 5 µl of L-15 on a MaTek well (MaTek #P35G-0-14C) followed by the addition of 5 µl of matrigel (BD #356231). The matrigel was allowed to polymerize at room temperature for ∼10 minutes and Z-stacks of salivary glands were acquired using a 3i spinning disk confocal microscope (Zeiss Axio Observer Z1 microscope with Yokogawa CSU222 spinning disk) ...
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bioRxiv - Molecular Biology 2021Quote: ... between 500.000 and 1 million GFP-mCherry double positive cells were sorted each day for 5 consecutive days using two cell sorters (BD FACSAriaII SORP and BD FACSAriaIII). Sorted cells were pooled ...
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bioRxiv - Cell Biology 2022Quote: ... filtered with a 3 μm filter and sorted for YFP expression into a 96 well plate (5 and 10 events; 48 wells each) (FACSARIA III, BD Biosciences). Individual plaques were screened by PCR and all modified loci were sequenced to confirm correct modification ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were transferred to a 50 ml tube and 9 ml LB (10 g/l Peptone (OXOID, LP0037), 10 g/l NaCl (ACROS, 207790010) and 5 g/l Yeast Extract (BD, 211929)) were added with 50 μg/l kanamycin (ACROS ...
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bioRxiv - Immunology 2022Quote: ... overlapping peptides (10µg/mL) or media alone for 60 hours at 37 °C and 5% CO2 with proliferation dye (Violet Proliferation Dye, BD Biosciences). Peptides were generated in 15mer format with 8 amino acid overlaps for the ID91 fusion antigen ...
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bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
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bioRxiv - Immunology 2022Quote: ... cells were washed 2x with FACs buffer at 1000xg for 5 minutes at room temperature and analyzed on a LSR II (BD Biosciences) at the Flow Cytometry Shared Resource at the University of Arizona ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 5-6 days and CFSE dilution of T cells was determined on a BD Canto II (BD Bioscience) and analyzed by FlowJo (v10.1 ...
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bioRxiv - Cancer Biology 2020Quote: ... T cells were analyzed for proliferation at day 5 via Cell trace dye (eBioscience) and inhibitory receptor expression (LAG3 and PD1) on a Fortessa II (BD Bioscience) and data analyses were performed via FlowJo (Tree Star).
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells (5 × 104 cells per well) were plated in 24-well culture plate and the top chamber (24-well insert; BD Bioscience) were seeded with MCF7 (2 × 104 cells ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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bioRxiv - Immunology 2021Quote: ... Spleen cells of immunized mice were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 mAb (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... Non-adherent cells transduced with PMG based vectors were typically sorted for top 5-20% ZS-green expressing cells once on a FACSAria III (BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: HeLa cells were trypsinized and resuspended in serum-free media supplemented with 5 mM EDTA prior to measurement on an Accuri C6 plus cytometer equipped with 488 and 640 nm lasers (BD Biosciences). Untransfected cells were gated out during analysis.