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Citations for Becton, Dickinson and Company :
3651 - 3700 of 8197 citations for 1 4 Dichloro 2 5 bis dichloromethyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cells were resuspended in DPBS containing 2% FCS and 1mM EDTA and immediately analyzed on a FACSCanto II or FACSymphony A1 Cell Analyzer (BD Biosciences). For yH2AX measurements in U2OS cells ...
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bioRxiv - Microbiology 2024Quote: ... The plates were incubated for 2 h at 37 °C before the bacteria were resuspended in a final volume of 100 µl MH broth (BD Biosciences). Serial dilutions in MH broth were carried out and 5 µl of each dilution was spotted in technical duplicates on MH agar plates supplemented with 50 µg/ml ampicillin for calculation of total number of colony forming units (CFUs ...
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bioRxiv - Bioengineering 2024Quote: ... for viability assessment and fixed with 2% paraformaldehyde (30 min incubation) until analysis by flow cytometry using an LSR Fortessa (BD Biosciences). For assessment of NP-associated fluorescence in a fluorescence plate reader ...
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bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were assayed for IL-2 using capture and biotin-labeled detection antibody pairs (BD Biosciences, Franklin Lakes, NJ). Detection was performed with HRP-conjugated streptavidin (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: Splenocytes or lung cells were cultured in presence or absence of 2 μL/mL of cell stimulation cocktail (eBioscience, 00-4975-93) and brefeldin A (BD, 555029) in RPMI complete media for 6 hours at 37°C in a humidified chamber and 5% CO2 ...
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bioRxiv - Biophysics 2024Quote: ... After incubation cell free supernatant was collected and subjected to sandwich ELISA using IL-2 ELISA kit (BD biosciences, CA, USA) as per manufacturer instruction ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and the cells were resuspended in 2 mL red blood cell lysis buffer (1X, BD Biosciences, 555,899), mixed by pipetting up-and- down ...
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bioRxiv - Cancer Biology 2024Quote: ... Final cell pellets were resuspended at a cell density of 2×106 cells/ml in 3% FBS in PBS and analyzed at the UTSW Flow Cytometry Core Facility (FACSCalibur, BD Biosciences). FlowJo software was used to analyze mean fluorescent intensity (RRID:SCR_008520).
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bioRxiv - Developmental Biology 2022Quote: ... The cells were incubated with phycoerythrin (PE)-conjugated Enpep antibody at 4°C for 60 min and then sorted by FACScan flow cytometry (Becton, Dickinson and company, Franklin Lakes, NJ).
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bioRxiv - Immunology 2020Quote: ... Cells were then surface stained for 30 min in the dark at 4°C with viability reagent (BD Horizon™ Fixable Viability Stain, BD Biosciences, San Diego, CA, USA) and a 29-color cocktail of monoclonal antibodies (mAbs ...
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bioRxiv - Cell Biology 2024Quote: ... All tubes were centrifuged for 4 minutes at 2,000 RPM and the cells were resuspended in 200 μL of Antibody buffer (BD Perm/Wash buffer + 10 μL goat serum) with or without BRN3A antibody (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-calreticulin (612136, dilution 1:1000), and mouse anti-GM130 (610823, dilution 1:1000) (BD Biosciences); mouse anti-α-tubulin (DM1A ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies were used: Early endosome antigen 1 (EEA1) at 1:500 (#610456; BD Biosciences); amyloid precursor protein (APP ...
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bioRxiv - Biochemistry 2020Quote: ... phosphorylated eNOS (Ser1177, 612393, 1:1000; BD Transduction, Franklin Lakes, NJ. Thr495, 612707, 1:1000, BD Transduction), PKC (sc-17769 ...
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bioRxiv - Microbiology 2020Quote: ... 1:50000 in 1% BSA PBS pH 7 and analysed by cytofluorimetry in a FACSCalibur (BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... 2.0 × 105 4T1 cells were mixed 1:1 with BD Matrigel Matix Growth Factor Reduced (BD Bioscience) and injected into the right mammary fat pad.
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bioRxiv - Physiology 2022Quote: ... and BLCs (PE Mouse Anti-NKX6.1: 1:40, BD Biosciences Cat# 563023, RRID:AB_2716792; and AF647 Mouse Anti-C-Peptide: 1:200 BD Biosciences Cat# 565831 ...
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bioRxiv - Systems Biology 2020Quote: ... and incubated with mouse anti-human CD63 primary antibody (BD Pharmingen 556019, 1:20 in 1% BSA) for 30 minutes at room temperature ...
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bioRxiv - Genetics 2023Quote: ... 10 g.L-1 of D-glucose (EMD Chemicals) and 15 g.L-1 of Bacto agar (BD Diagnostics)) directly from 15% glycerol (v/v ...
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bioRxiv - Microbiology 2023Quote: ... After 1 hr incubation on ice bacteria outgrowth was done for 1 hr in Luria-Bertani (BD) prior to selection on LB agar containing desired antibiotic (Sup ...
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bioRxiv - Neuroscience 2024Quote: ... dead cells were excluded with the help a DAPI viability dye (1 μg ml-1, BD Pharmingen). Single cells were gated for based on forward and side scatter parameters ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunostained with biotinylated antibodies as follows: anti-mouse IgG1 (1:100, BD Pharmingen 553441; A85-1), anti-mouse IgG2b (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with primary antibodies (NMDAR2A-1: 1000, #ab169873, Abcam; NMDAR2B-1:1000, #610417, BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: Bacteria were isolated from site #1 by plating water on Zobell Agar (1 g yeast extract (BD), 5 g bacto-peptone (BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34-eFluor610 (1:50, cone 4H11; eBioscience) and CD144-V450 (1:50, clone 55-7H1; BD Biosciences). 7AAD (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: 1° Rb – α-active caspase 3 (BD Pharmingen, Cat: 559565, 1:500); 2° Dk – α-Rb-IgG Dylight 550 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
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bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... infected mouse brain for flow cytometry were obtained as described.(Posel et al., 2016) Isolated cells were incubated overnight (5 hours for microglia analysis) in GolgiPlug and GolgiStop (BD Bioscience #555029 and #554724) in RPMI media containing HEPES and 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were resuspended in PBS + 5% FBS and analyzed via Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Fluorescence minus one (FMO ...
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bioRxiv - Microbiology 2021Quote: Lorikeet intestinal contents collected at necropsy were plated on reduced Trypticase Soy Agar (TSA II) with 5% sheep blood (BD BBL, Franklin Lakes, NJ) and incubated anaerobically (5% CO2 ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were cultured in complete media (106 cells /ml) for 72h in anti-CD3/anti-CD28 (5 μg/mL; BD Biosciences, San Jose, USA) pre-coated 96-flat-well plates (BD Biosciences) ...
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bioRxiv - Genomics 2020Quote: ... at a concentration of 100μg/ml starting 5 days after transfection for the following 7-9 days before single cell sorted using BD FACSAria II (BD Bioscience, San Jose, CA) for clonal cell line generation ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Microbiology 2021Quote: ... This was done by treating both the PDMS and glass surface with 5-10 sweeps of a portable laboratory corona device (model BD-20ACV, Electro-Technic Products). After treatment the chip was placed carefully onto the glass slide and gently tapped to facilitate full contact between the PDMS and glass surface ...
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bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Bioengineering 2023Quote: Splenocytes from 125Tg B6 and WT B6 control mice were stained with CellTrace Violet and cultured for 3 days35 in complete RPMI alone or stimulated with 5 ug/mL anti-mouse anti-CD40 (BD Pharmingen clone HM40-3) in the presence or absence of conjugates described above at 150 nM concentrations ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with ice-cold DPBS plus 5 mM EDTA and stained with annexin V and PI (BD Biosciences, Franklin Lakes, NJ, USA) for 15 min at RT ...
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bioRxiv - Bioengineering 2023Quote: ... pneumoniae serotype 3 (Sp3) (ATCC; Manassas, VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... the frozen bacterial stock was thawed in 37°C water bath and cultured at 37°C in 5% CO2 in Todd Hewitt broth (BD Biosciences, San Jose, CA) to mid-logarithmic phase ...
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bioRxiv - Developmental Biology 2023Quote: ... cells filtered into 5 ml polystyrene tubes and analyzed by flow cytometry using a FACS Caliber flow cytometer and FlowJo software (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Genetics 2024Quote: ... the pigs were fasted overnight and 5∼10 mL blood was sampled from the jugular vein using a vacutainer (Cat no. 367986, BD Vacutainer SST™, USA) and transported to the laboratory within 10 min ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were then cultured at 1.106 cells/well in 12 well/ plate that were coated overnight one day earlier with anti-CD3 mAb (clone145-2C11, BD pharmingen, 5 µg/ml) within 2 ml/well of complete medium (RPMI 1640 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked with 5% BSA in PBS and BrdU was then detected with a mouse anti-Brdu primary antibody (BD Biosciences, Clone B44, 347580) and goat anti-mouse AlexaFluor-488 conjugated secondary (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... The bottom layer of soft agar was prepared by mixing 2 × growth medium and 1.5% noble agar (BD Biosciences, San Jose, CA) at a 1:1 ratio and the mixture was poured into 6-well plates ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Microbiology 2020Quote: ... Strains were precultured during 48h in 300 μL of Synthetic Defined (SD) medium (Warringer et al., 2011) with some modifications (2% glucose, 0.14% Yeast Nitrogen Base (BD Difco™, U.S.A.), 0.5% ammonium sulfate ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...