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Citations for Becton, Dickinson and Company :
3601 - 3650 of 4205 citations for Recombinant Mouse IFNG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Membranes containing the transferred proteins were rinsed and blocked in 0.1% Tween-20 in Tris-buffered saline (TBST) containing 5% nonfat milk (BD Biosciences) at RT for 1 h ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
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bioRxiv - Biochemistry 2024Quote: ... AtHSP90-3 and ASDURF were transferred to the pGADT7-GW and pGBKT7-GW destination vectors using Gateway LR Clonase II enzyme mix to produce fusion proteins of the Gal4-activation domain (AD) and GAL4 DNA-binding domain (BD), respectively ...
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bioRxiv - Immunology 2023Quote: ... Intracellular staining for cytoplasmic and nuclear proteins were performed with Transcription Factor Staining Buffer kit according to manufacturer’s instructions (BD Pharmingen). Dead cells were excluded by DAPI (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are as follows and were used according to manufacturer’s protocol: PE-Cy7 Mouse Anti-Human CD3 (Cat 563423; BD Pharmingen), PE Mouse Anti-Human CD4 (Cat555347 ...
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of total IgE were quantified with BD OptEIA™ Mouse IgE ELISA Set (BD Biosciences, San Chose, Canada) with samples diluted 1:100 according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... co-culture supernatants were thawed at room temperature for 1 hr and cytokines stained with mouse Th1/Th2/Th17 cytokine kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... 2mM EDTA and 0.05% NaN3): PerCP-Cy™5.5 mouse anti-human CD19 (1:40 dilution, Clone HIB19, BD Pharmingen) (for PBMC samples only) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibody in incubation buffer overnight at 4°C with mouse anti-CtBP2 antibody (1:50, BD Transduction ...
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bioRxiv - Immunology 2022Quote: ... cells were first stained for surface markers and then fixed and permeabilized using Intracellular Fixation and Permeabilization Buffer Set (eBioscience) before incubation with fluorochrome-conjugated mAbs against mouse IFNγ (clone XMG1.2, BD Bioscience). Flow cytometry was performed on LSRFortessa (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... and IL-17A) were simultaneously measured using the mouse Th1/Th2/Th17 cytokine cytometric bead array (CBA) kit (BD Biosciences). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated in blocking buffer (0.1% Triton X-100 plus 10% normal goat serum in DPBS) for 20 minutes followed by overnight incubation in primary antibodies at 4°C (mouse anti-GM-130, BD Transduction laboratories #610822 1:250 ...
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bioRxiv - Cell Biology 2019Quote: Anti-Cav-1 rabbit polyclonal (cat # 610059) and anti-phos-Y14-Cav-1 mouse monoclonal (cat # 611338) antibodies were from BD Transduction (San Jose ...
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bioRxiv - Immunology 2019Quote: ... Serum was also collected by cardiac puncture for measurement of IL-6 by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... xenografts and mouse brain tissue were embedded in 3-4% (w/v) Difco Noble agar (BD Biosciences, San Jose, California) in distilled water ...
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bioRxiv - Bioengineering 2019Quote: ... Sections were co-incubated overnight with rat monoclonal anti-mouse MAdCAM-1 (1:1000; clone MECA-367 from BD Biosciences) and goat anti-collagen-type IV (1:1500 ...
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bioRxiv - Immunology 2019Quote: ... cells from different stimulation conditions were permeabilized and fixed according to the manufacturer’s instructions with the FOXP3 permeabilization kit (Foxp3/Transcription Factor Staining Buffer Set ebioscience) and then labelled with an anti-Ki-67 antibody (clone B56, mouse IgG1; BD) and DAPI was added before flow cytometry analysis.
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bioRxiv - Immunology 2019Quote: ... Samples were then washed twice in the FACS buffer and incubated with anti-mouse IgG1-PE (BD Biosciences, ref.#550083) and anti-rabbit IgG-AlexaFluor647 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated for 1 h at RT with primary antibody used as follows: mouse anti-EEA1 (BD Biosciences: 610457) 1 µg/mL ...
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bioRxiv - Microbiology 2019Quote: Cytokines were measured in culture supernatants using Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD Bioscience, San Jose, CA, USA). The kit was used for the simultaneous detection of mouse monocyte chemoattractant protein-1 (MCP-1) ...
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bioRxiv - Immunology 2019Quote: ... Cell surface markers were immunolabeled with anti-Gr-1 (PE Rat Anti-Mouse Ly6G and Ly6C, BD Pharmingen, Franklin, NJ), F4/80 (Anti-F4/80 PE ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed 3 times with PBS-T and 50μl of either biotinylated rat anti-mouse IgG1 (1:500, BD Bioscience), rat anti-mouse IgG2a (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor xenografts were established in 6-week-old NOD SCID mice via subcutaneous injection into the dorsal region of each mouse with 1 x 107 cells/0.15 ml Hank’s balanced salt solution containing Matrigel® (BD Biosciences). Experiments were performed at five mice in each group (vector control ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used for immunostaning the cells were : CD3+ (Mouse anti-pig CD3ε Alexa Fluor 647; BD Pharmingen, catalog no: 561476), CD4+ (Primary antibody ...
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bioRxiv - Immunology 2021Quote: ... and then stained with the fluorochrome-labeled anti-mouse CD3/CD4/CD8/CD44/CD62L mAbs (all purchased from Biolegend except CD44 from BD). Cells were then treated with Cytofix/Cytoperm (BD ...
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bioRxiv - Neuroscience 2020Quote: ... to improve staining quality and with 2,5 mL per sample of each of the following anti-mouse mAbs: Ly6G BB515 (Clone: 1A8; BD, USA), CD45 BV786 (Clone ...
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bioRxiv - Neuroscience 2020Quote: ... mouse primary at 1/100, Dako; CldU: rat primary at 1/1,000, Accurate Chemical; IdU: mouse primary at 1/1,000, BD Biosciences) and with a rabbit anti GFP (Millipore ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used in this study: mouse monoclonal antibodies against Rab6 (Schiedel et al., 1995) and Ku80 (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were activated by seeding in 96-well plates pre-coated with anti-mouse CD3e (1 μg/ml working concentration, Clone: 145-2C11, BD) and anti-mouse CD28 (2 μg/ml working concentration ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in T cell media supplemented with soluble anti-mouse CD28 (2 μg/ml, Clone: 37.51, BD Pharmingen) and 30 U/ml rhIL-2 (Roche ...
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bioRxiv - Bioengineering 2021Quote: ... at 2×106 cells/ml in wells coated with anti-mouse CD3e (1 μg/ml, Clone: 145-2C11, BD Pharmingen).
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846; 1:200 mouse anti-Map2, BD Pharmagen ...
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bioRxiv - Cell Biology 2021Quote: ... 1:500 for immunofluorescence or IF) and mouse anti-Golgin245 mAb (#611280, 1:200 for IF) were purchased from BD Bioscience ...
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bioRxiv - Neuroscience 2019Quote: ... Drug solutions were IP injected in 0.1 ml solution/10 g of mouse using a fine needle insulin syringe (BD 324911, BD Bioscience). On average ...
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bioRxiv - Bioengineering 2021Quote: ... live/dead stain in PBS for 15 min on ice before washing and re-suspending in 2x human/mouse Fc block in stain buffer (BD) followed by surface-staining with monomeric GFP and anti-human CD45 AF700 (Biolegend clone HI30) ...
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bioRxiv - Systems Biology 2021Quote: ... Similarly detached hCMEC/D3 cells were incubated with APC-conjugated mouse monoclonal anti-BCRP (1:100, BD Biosciences, Oxford, UK), or PE-conjugated mouse monoclonal anti-MDR1A (1:100 ...
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bioRxiv - Microbiology 2020Quote: Mouse cytokines in the culture supernatants were measured with a BD OptEIA ELISA kit (BD Biosciences, Franklin Lakes, NJ, USA) as described previously (84) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C with primary antibodies (Guinea-pig anti-VGAT, Synaptic Systems 131004, 1μg/mL, 1:200 and mouse anti-mGluR1, BD Pharmingen,0.5mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Immunocytochemical staining using a rat monoclonal anti-mouse CD31 antibody (1:100; clone MEC 13.3; BD Pharmingen, San Jose, CA) was used to detect endothelial cells in the mouse heart ...
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bioRxiv - Microbiology 2021Quote: ... samples were re-suspended in 25 μl of anti-mouse CD16/CD32-Fc/Block (BD Bioscience, San Jose, CA, USA), incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-EEA1 mAb (#610456; 1:500 for IF) and mouse anti-GM130 mAb (#610823; 1:500 for IF) were from BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 300µL of PBS + 1% v/v FBS and 150µL were added to 2µL of Mouse BD Fc Block (BD Biosciences 553142). Homogenates in Fc block were incubated for 15 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... the sections were incubated with HypoxyprobeTM rabbit anti-pimonidazole antibody (1:250; hpi) for pimonidazole staining or rat anti-mouse CD31 antibody (1:250; BD) for CD31 staining overnight at 4℃ ...
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bioRxiv - Cell Biology 2020Quote: ... ELISA kits were used to quantify secreted TNF (Ready-SET-Go! Mouse TNFα Kit, eBiosciences,88732488) and IL-6 (BD OptiEIA ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse Anti-human KDR (KDR-PE, FAB357P, R&D) and Mouse Anti-Human NRP-1 (NRP-1-PE, Material Number: 565951, BD) antibodies were used at a ratio of 1:20 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation in blocking buffer (1% anti-mouse-CD16/CD32, 2.4 G2, 2% FBS, 5% NRS,1% triton x100 and 2% NMS in HBSS; BD Bioscience) for 1h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Fc-blocked (1 μg/mL; eBiosciences) BALF cells were stained with anti-mouse SiglecF-PE (0.3 μg/mL; BD Pharmingen), CD11c-APC (0.3 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked with 5% milk in TBS 0.1% Tween 20 and incubated with: mouse anti-P-cad (1:2500 BD, 610228), mouse anti-MRTF-A (1:1000 Santa Cruz ...