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Citations for Becton, Dickinson and Company :
3601 - 3650 of 4529 citations for Recombinant Human ERBB2 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were then incubated with FITC-conjugated α6 integrin antibody and PE-labelled CD71 antibody (BD Biosciences, San Jose, USA) at room temperature for 0.5 h ...
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bioRxiv - Physiology 2024Quote: ... + 10% FCS + 1% penicillin/streptomycin and the purity of isolated neutrophils was confirmed as >98% CD45+ CD15+ via flow cytometry (anti-CD45-FITC, 555482 and anti-CD15-PE, 555402, BD Biosciences).
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bioRxiv - Pathology 2024Quote: ... APC-conjugated anti-Ly-6G (17-9668, eBioscience), FITC-conjugated anti-CD3 (11-0032-82, eBioscience) and PE-conjugated anti-Siglec-F (552126, BD Biosciences) antibodies for 30 min at 4°C and analyzed on a BD FACSCanto II apparatus.
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bioRxiv - Immunology 2024Quote: ... Cell apoptosis was measured by co-staining cells with Annexin V and 7-AAD using the PE Annexin V Apoptosis Detection Kit I (BD Biosciences), according to manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... and then resuspended in 500 µL PBS before measuring TMRM intensity using the PE bandpass filter of a FACSCanto (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... TCR transduction efficiency was measured by flow cytometry using the PE a-mouse TCRβ antibody (clone H57-597, BD Biosciences 553172) and anti-PE MicroBeads (Miltenyi Biotec 130-048-801) ...
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bioRxiv - Biophysics 2022Quote: ... samples were first resuspended in 15.5 μL PBS/0.1% adBSA and incubated with 0.5 μg of rat anti-mouse CD9-PE (Clone: KMC8, IgG2a, κ lot 7268877, BD Biosciences) or matched isotype antibodies (Rat IgG2a ...
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bioRxiv - Biophysics 2023Quote: ... Enrichment of surface expression in PE-selected cells was confirmed by flow cytometry using a BD FACS LSR II cytometer (BD Biosciences) and analyzed with FlowJo software (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Immunology 2023Quote: ... together with PE-anti-PD-L2 (clone TY25, 1:20) and its isotype control PE-Rat IgG2a,κ (clone R35-95, 1:20) were purchased from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... GFP expression in peripheral blood B and T cells was screened using anti-CD19-PE (BD, clone 1D3, dilution 1/100), anti-CD3e-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used: Anti-CD166 mouse monoclonal antibody conjugated with PE (phycoerythrin) at 5 uL (BD Biosciences #559263), anti-LRIG1 sheep polyclonal unconjugated antibody at 2.5 ug (Invitrogen #PA5-47928) ...
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bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
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bioRxiv - Cancer Biology 2023Quote: ... Annexin V was coupled to FITC (#556420, BS bioscience) for the 1057 and MDA-MB-435S cell lines and to PE (#556421; BD bioscience) for the other cell lines ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents: PE-conjugated rat anti-mouse Siglec-F (E50-2440; BD Biosciences), PE-TxR or PE-Cy5-conjugated rat anti-mouse CD11b (M1/70.15 ...
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bioRxiv - Immunology 2022Quote: ... ProT2 MHC Class II monomers for HLA-DRB1*15:01 (S751-767 NLLLQYGSFCTQLNRAL) were purchased from ProImmune and conjugated to Streptavidin-PE (BD Biosciences) at a molar ratio of 8:1 monomer:S-PE ...
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bioRxiv - Neuroscience 2024Quote: ... fixed and permeabilized with the FoXP3 Transcription Buffer Kit (eBioscience) and intracellularly stained with PE-CF594-conjugated anti-Foxp3 (BD Biosciences) and PE-conjugated anti-IL-10 (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... inguinal lymph nodes close to the site of inoculation were harvested and cells were stained with fluorophore-conjugated antibody PE-anti-CD11c (clone HL3, BD Pharmingen). Analysis was performed using the FlowJo Software and results were expressed as the percentage of CFSE+/CD11c+ cells.
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bioRxiv - Molecular Biology 2024Quote: ... unsorted, sorted, and depleted fractions, were labelled with mouse anti-ASGPR1-PE (1:20 dilution, to detect porcine hepatocytes) (BD Pharmigen), rat anti-OC2-2F8 ...
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bioRxiv - Microbiology 2024Quote: ... Culture supernatants were mixed with the indicated cytokine beads and PE-conjugated detection antibodies from the Mouse Th1/Th2/Th17 CBA Kit (BD Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... using 1:20 diluted plasma. FLT3L ELISA (cat. # DY308, R&D) and GMCSF ELISA (cat. # 555126, BD) were performed with 1:2 diluted plasma ...
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bioRxiv - Neuroscience 2022Quote: ... GDNF (10 ng.mL-1, R&D Systems 248-BD/CF, 257-NT/CF and 212-GD/CF) and doxycycline hyclate (2 µg.mL-1 ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 30 min on ice with biotinylated and Alexa Fluor 647-conjugated recombinant soluble S and PD-1-BB515 (EH12.1, BD Horizon, 1:100) in 2% FBS and 2 mM EDTA in PBS (P2) ...
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bioRxiv - Cell Biology 2022Quote: ... the protein transport inhibitor was added (BD GolgiStopTM ...
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bioRxiv - Immunology 2021Quote: ... and protein transport inhibitor cocktail (BD, USA) for 5h ...
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bioRxiv - Immunology 2024Quote: ... GolgiStop Protein Transport Inhibitor (BD Bioscience, UK) was added ...
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bioRxiv - Developmental Biology 2021Quote: ... were plated in buffer (PBS with 0.5% bovine serum albumin and 1 mM EDTA) and non-specific Fc binding was blocked via incubation with mouse anti-rat CD32 (BD Biosciences #550271; 1:200) for 30 minutes on ice ...
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bioRxiv - Immunology 2020Quote: The following rat anti-mouse antibodies were used for leukocyte analysis within each tissue: purified CD16/CD32 Fc blocking antibody (clone 2.4G2; BD Pharmingen™, San Jose, CA), PerCP-Cy™5.5 Ly6G antibody (clone 1A8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
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bioRxiv - Cancer Biology 2023Quote: ... APC-eFluor780-conjugated cKit and BV605-conjugated Fc Gamma (CD16/CD32) for 30 minutes followed by fixation and permeabilization using BD Cytofix/Cytoperm (BD Biosciences, San Jose, California). Intracellular staining using FITC-conjugated KI-67 was performed overnight at 4 degrees ...
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bioRxiv - Microbiology 2023Quote: ... spun for 10 minutes and then resuspended in 50 μL of a 1:200 dilution of Fc block (CD16/CD32, clone 2.4G2, BD Biosciences, cat. 553141, 0.5mg/mL) in FACS buffer (PBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Immunology 2021Quote: ... Spike protein conjugated to Alexa 647 and Spike protein conjugated to Alexa 488 and Brilliant Staining buffer (BD Horizon). Samples were then incubated on ice for an additional 30 minutes before they were washed twice with P2 and fixed in a final concentration of 1% paraformaldehyde for 15 minutes at room temperature ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... a master mix containing a panel of fluorophore- or biotin-labeled antibodies was mixed at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences) at 2X ...
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bioRxiv - Immunology 2021Quote: ... prior to staining with a 2X master mix containing fluorophore-labeled antibodies at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences), and added to suspended cells at a final staining concentration of 2 x 108 cells/mL for 30-45 min ...
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bioRxiv - Immunology 2021Quote: ... prior to staining in the dark for 30 min on ice with fluorophore or biotin-labeled monoclonal antibodies prepared in brilliant stain buffer (BD Biosciences) ‘cocktail’ at pre-titrated quantities ...
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bioRxiv - Genomics 2020Quote: ... A 2X agonist solution (100 mM KCl or 200 μM AMPA or 10 μM ionomycin) was prepared and APC-CY7-labeled CompBead compensation particles (BD Biosciences) were added to the solution before putting it in the second injection syringe ...
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bioRxiv - Synthetic Biology 2020Quote: ... The median fluorescence intensity (MFI) of SSHEL particles containing AlexaFluor488-labeled Ab31889 was measured using flow cytometry (FACSCanto II, BD Biosciences) and quantified using BD FACSDiva software ...
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bioRxiv - Neuroscience 2020Quote: ... Mononuclear cells from the remaining five brains were labeled with APC-Cy7 rat anti-CD11b antibody (BD Pharmingen, Cat. No. 557657) for 30min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... CD34+CD38-cells from healthy controls and MDS patients cells were also labeled with the BV605-conjugated anti-CD38 (BD Biosciences) (#562666) ...
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bioRxiv - Cancer Biology 2022Quote: ... BM cells were surface labeled for B220 and sIgµ expression and B220+sIgµ- cells were sorted using a FACS Aria III (BD Bioscience). Sorting efficiency was routinely above 95 % ...
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bioRxiv - Cell Biology 2022Quote: Bone marrow cells from mice were labeled with a cocktail of biotin-conjugated lineage marker antibodies in combination with Streptavidin Particles Plus (BD, 557812) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... resuspended in 1X Annexin binding buffer and stained with FITC-labeled Annexin V and PI (BD Pharmingen, San Diego, CA, US). Cells were then analyzed on BD FACS Aria™ (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... cryo-preserved PBMCs were first thawed at 37°C in a water bath and washed with an RPMI medium containing 10% fetal bovine sera (FBS) following incubation with fluorescently-labeled antibodies (BD Biosciences) against cell surface markers for CD3 (PE-Cy7) ...
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bioRxiv - Genomics 2022Quote: Whole Transcriptome Analysis (WTA) on MULTI-seq labeled cells was performed using the BD Rhapsody Single-Cell Analysis System (BD Biosciences). In total 9 samples were processed of mice that were injected with colon cancer organoids ...
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bioRxiv - Immunology 2022Quote: ... 20 μm paraformaldehyde fixed lung sections from Mtb-mCherry infected I-Tomcat Ai6 and Sp140−/− I-Tomcat Ai6 mice were stained at 4°C overnight with BV421-labeled SIRP□ (P84, BD Biosciences), Pacific Blue–labeled B220 (RA3-6B2 ...
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bioRxiv - Microbiology 2024Quote: ... The initial and final proportions of CFP- and YFP-labeled cells were measured by flow cytometry on the BD LSRFortessa SORP cell analyzer (BD Biosciences). The sample was excited with lasers at 447 nm (75 mW) ...
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bioRxiv - Immunology 2024Quote: ... and CD45.2+ peripheral blood leukocytes were FACS-sorted and labeled using the BD Rhapsody Single-Cell Multiplexing CTT Kit (BD Biosciences, 626545). On average ...
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bioRxiv - Cell Biology 2023Quote: ... The nuclei were washed again and resuspended in sorting buffer with 0.2 U/μl Protector Rnase inhibitor prior to cell sorting to separate the PCM1-labeled fractions using a FACS cell sorter (BD Aria). The sorted nuclei were then pelleted at 3000 × g for 10 minutes before mRNA isolation using the Direct-zol RNA Microprep kit (Zymo research R2060) ...