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Citations for Becton, Dickinson and Company :
3551 - 3600 of 3898 citations for IGF1 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... transferred to flow cytometry tubes and stained with 1:100 mouse IgG2a CD31-647 clone M89D3 (BD Bioscience, 558094, lot 8145771) for 30 min on ice then washed once with DPBS ...
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bioRxiv - Cell Biology 2019Quote: ... Tet1KO and WT reprogramming intermediates were incubated 30 min on ice with Phycoerythrin (PE)-conjugated Mouse anti SSEA1 (1:100, Cat.#FAB2155P-025, R&D) and analysed on CANTO I (BD Biosciences). PGCLCs at day8 were sorted using FITC Mouse anti-SSEA1 (1:20 ...
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bioRxiv - Immunology 2019Quote: Lymphocytes or purified splenic B cells were washed in PBS with 1% BSA and incubated with 1mg/ml purified rat anti-mouse CD16/CD32 (Fc block, BD Pharmingen) at 4°C for 15 min ...
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bioRxiv - Immunology 2019Quote: ... Bound antibody was revealed by staining with a FITC- or PE- conjugated anti-mouse IgMa (Igh-6a) monoclonal antibody (BD Pharmingen). Bone marrow progenitor B cells were defined as B220+ ...
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bioRxiv - Immunology 2020Quote: ... and 14 days post-infection (DPI) was determined by culturing serially diluted mouse lung homogenates on 7H10 agar plates (BD Biosciences) at 37°C for 2-3 weeks ...
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bioRxiv - Immunology 2019Quote: ... incubated with anti-mouse IgG1-fluorescein isothiocyanate for 45 min at room temperature (1:100 dilution; BD Pharmingen; cat. no. 553443), and washed three times with PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell suspensions from APEL vascular differentiations were incubated with mouse anti-human CD31-APC (eBioscience, San Diego, CA) and CD146-PE (BD Biosciences) antibodies for 30 min on ice ...
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bioRxiv - Microbiology 2019Quote: ... the cells were washed once with FACScan buffer and then stained with PE-conjugated monoclonal rat anti-mouse CD4 (BD Pharmingen) and/or APC-conjugated monoclonal rat anti-mouse CD8 (eBioscience) ...
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bioRxiv - Immunology 2020Quote: ... cover slips were washed 3 times in 1xPBS and incubated with secondary antibodies (anti-rabbit-AF647, anti-mouse-AF488 (BD Bioscience)) diluted 1:1000 in antibody diluent for 1 hour at RT ...
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bioRxiv - Immunology 2019Quote: Cytometric bead array (CBA) was performed using the mouse soluble protein master buffer kit combined with the appropriate CBA flex sets (BD Biosciences), per manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... B cells were pre-incubated during 15 minutes with 0.5 μg/ml anti-mouse CD16/CD32 (Clone 2.4G2, BD Pharmingen, ref 553142) in FACS buffer (PBS supplemented with 2% FCS and 2 mM (Ethylenedinitrilo)tetraacetic acid (EDTA ...
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bioRxiv - Immunology 2019Quote: ... Cells were further incubated for an additional 30 minutes on ice with a mixture of the following antibodies: Alexa Fluor 700 rat anti-mouse CD45R (B220, clone RA3-6B2, BD Biosciences), FITC rat anti-mouse CD43 (Clone S7 ...
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bioRxiv - Molecular Biology 2019Quote: ... The HLA-DQ cell surface expression was performed by cytofluorimetric analysis using the FACSAria III and DIVA software with FITC mouse anti-human HLA-DQ antibody (BD Biosciences). The quantitation of specific transcripts was performed by qRT-PCR as described in [9] ...
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bioRxiv - Cancer Biology 2019Quote: ... cKIT and PDGFRA expression was assessed using mouse anti-human CD117 (PE-Cyanine7, clone 104D2, #25-1178-42, eBioscience) and CD140a (PE, clone αR1, #556002, BD Pharmingen) respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 h in 5% milk/TBS Tween-20 solution and incubated for 1 h with anti-TOP2β (1:5,000, mouse anti-human, Clone 40/Topo IIβ, RUO – 611492, BD Biosciences-US) or anti-TOP2α (1:10,000 ...
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bioRxiv - Cancer Biology 2019Quote: ... were stained with monoclonal rat anti-BrdU (1:500) (Clone BU1/75 (ICR1) (AbD Serotec) and monoclonal mouse anti-BrdU (1:500) (Clone B44, 347580 (BD Biosciences)) respectively for 1 hour in 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed and permeabilised cells were washed with PIPES buffer and stained with mouse anti-e-cadherin (Clone 36/E; BD Bioscience) in 5% goat serum and 0.1% saponin containing PIPES buffer for 1 h washed and stained with secondary antibodies coupled to Alexa 647 for 45 min ...
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bioRxiv - Neuroscience 2020Quote: ... Cas9 mouse NPCs where transduced at a MOI 40 and allowed to expand for 10 days prior to FACS (FACSAria, BD Biosciences). Cells were detached and resuspended in basic culture media (media excluding growth factors ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with fluorochrome-conjugated (BV421, BV711, FITC, PE, PE-Cy7, allophycocyanin [APC], APC-Cy7) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed with PBS containing 5 mM EDTA and 3% FBS (FACS Buffer) and then blocked with 1% anti-mouse CD16/CD32 (BD biosciences), 5% normal rat serum and 5% normal mouse serum in FACS buffer for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 72 h at 4°C in mouse anti-PKCδ primary antibody (1:1000, BD Biosciences, 610397) in blocking solution containing 1.5% NGS ...
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...
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bioRxiv - Cell Biology 2020Quote: ... The presence of IL-6 in the media was then determined by ELISA using the Mouse IL-6 OptEIA™ ELISA set from BD biosciences (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... rat anti-Foxp3 (1:100 dilution, clone FJK-16s, eBioscience) and mouse anti- CCR4 (1:100 dilution, clone 1G1, BD Biosciences), were applied at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... rat anti-Foxp3 (1:400 dilution, clone FJK-16s, eBioscience) or mouse anti-CCR4 (1:100 dilution, clone 1G1, BD Biosciences), at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently fluorescently labeled for 30 minutes on ice with the following antibodies and reagents: PE-conjugated rat anti-mouse Siglec-F (E50-2440; BD Biosciences), PE-TxR-conjugated rat anti-mouse CD11b (M1/70.15 ...
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bioRxiv - Developmental Biology 2022Quote: ... the secondary antibody reaction was carried out using FITC Rat anti-mouse IgM antibody (553408; BD Biosciences, Franklin Lakes, NJ, USA) at 1:200 dilution for 30 min on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... Hbbth3/+ and p21-/-/Hbbth3/+ mice and stained for Ki-67 using the Mouse Anti-Ki-67 Set (BD Biosciences, Cat# 556026). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... TCR expression on transfected Jurkat cells was assessed by staining with phycoerythrin (PE)-conjugated mouse anti-human CD3 (BD Pharmingen 555333); TCR expression was unaltered by Csk overexpression.
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bioRxiv - Cancer Biology 2022Quote: About 2×105 cells per sample were blocked using Purified NA/LE Rat Anti-mouse CD16/CD32 Clone 2.4G2 antibody (BD Biosciences, #553140) followed by incubation with 1 μl of specific antibodies for 30 min on ice in the dark ...
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bioRxiv - Molecular Biology 2022Quote: Concentrations of TNF-α and IFN-γ in sera on day 21 after allogeneic BMT were measured with specific anti-mouse ELISA kits from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... T cell receptor Vβ chains of splenic CD4+ T cells from CKO and control mice were assessed using a commercial mouse TCR Vβ screening panel (BD Biosciences). Flow cytometry data collection was performed on an LSR II (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with rat anti-BrdU/CIdU (BU1/75) monoclonal antibody (Novus, NB500-169) and mouse anti-IdU monoclonal antibody (BD, 347580). The secondary antibodies were AlexaFluor Cy3-conjugated goat anti-rat and AlexaFluor 488-conjugated goat anti-mouse respectively ...
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bioRxiv - Cell Biology 2019Quote: ... blocked for 1 hr in 1% BSA in TBS and then incubated for 1 hr with mouse anti-NF-KB p65 antibody (BD biosciences) at 1:1000 dilution in 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of antibody was quantified in plates coated in triplicate with two-fold dilutions of an appropriate purified mouse Ig isotype standard (IgG and IgA; BD Biosciences), starting at 0.5 μg/μl ...
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bioRxiv - Neuroscience 2020Quote: ... dissected tissues were then incubated overnight with rabbit anti-Afg3l2 (1:250, Antibody plus CSB-PA04005A0Rb) and mouse anti-cox4 (1:1000, BD Biosciences) antisera ...
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bioRxiv - Immunology 2020Quote: PBMC were labelled for FACS staining with mouse anti-human antibodies directed against lineage markers (Vioblue-CD3, CD14, CD19, CD56 and CD11c) (BD Biosciences), APC-Vio770 HLA-DR ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein (51)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk for 2 h ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk in each channel for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse xenograft tumors were generated by injecting 106 4T1 cells resuspended in 20% collagen I (BD Biosciences, Franklin Lakes, NJ, USA) in PBS into the lower-left mammary gland of 8-week-old BALB/c female mice ...