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Citations for Becton, Dickinson and Company :
3551 - 3600 of 4109 citations for Aprataxin And PNKP Like Factor APLF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... fixable viability dye was included with the surface staining antibodies (20 minutes, RT) and intracellular staining used the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: About 2×105 cells per sample were blocked using Purified NA/LE Rat Anti-mouse CD16/CD32 Clone 2.4G2 antibody (BD Biosciences, #553140) followed by incubation with 1 μl of specific antibodies for 30 min on ice in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then cultured on anti-hamster IgG coated plates in the presence of hamster anti-CD3 epsilon and anti-CD28 antibodies (BD Biosciences), neutralizing anti-IL-4 antibody (eBioscience) ...
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bioRxiv - Cancer Biology 2022Quote: ... barcoded and permeabilized as previously described.31 The samples were stained with the indicated antibodies and analyzed with a BD LSR Fortessa cytometer (BD Biosciences) equipped with 488 nm ...
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bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used herein at 1:200 dilution unless otherwise indicated: Fixed viability stain-700 (FVS700, BD, 1:1000), anti-IL-17A (TC11-18H10 ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... for 20 minutes and then with a blood antibody cocktail for 30 minutes in the dark at 4 degrees: anti-CD45 BB515 (BD Horizon), anti-CD14 PerCP-Cyanine5.5 (eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Cancer Biology 2022Quote: ... or Jurkat (CD19-) cells were stained with a phycoerythrin (PE)-labelled anti-CD47 (B6H12 clone) or isotype control antibody (BD Biosciences). The cells were pre-treated for 1 h with TG-1801 or an anti-human CD47 (B6H12 clone ...
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bioRxiv - Neuroscience 2021Quote: ... and their Fc receptors were blocked for 10 min at 4 °C with anti-CD16/CD32 antibodies (10 μg/mL: BD Biosciences). After blocking ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with fluorochrome-conjugated antibodies for 30min at 4°C in the dark in brilliant stain buffer (BD Biosciences). Prior to fixation and permeabilization for intracellular staining with Cytofix/Cytoperm (BD Bioscience) ...
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bioRxiv - Immunology 2020Quote: ... The cells were incubated for one hour at 4°C with a cocktail of fluorochrome-labelled antibodies consisting of anti-CD3-BV510 (cat. no: 563024, BD Biosciences), anti-B220-APC-Cy7 (cat ...
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bioRxiv - Cell Biology 2019Quote: ... blocked for 1 hr in 1% BSA in TBS and then incubated for 1 hr with mouse anti-NF-KB p65 antibody (BD biosciences) at 1:1000 dilution in 1% BSA ...
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bioRxiv - Immunology 2019Quote: ... Cells were initially stained with fluorochrome-conjugated antibodies against surface receptors (see Table S1) in BD Brilliant stain buffer (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... dissected tissues were then incubated overnight with rabbit anti-Afg3l2 (1:250, Antibody plus CSB-PA04005A0Rb) and mouse anti-cox4 (1:1000, BD Biosciences) antisera ...
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bioRxiv - Microbiology 2021Quote: Nectin-1 was detected on the surface of cells by staining them with anti-nectin-1 antibody CK41 conjugated to PE (BD Biosciences). HVEM was detected on the surface of cells using the anti-HVEM polyclonal antibody ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in PGB for 25 min with antibodies against Lewis-X (1:20, LeX/CD15 FITC, MMA; BD Bioscience), CD133-APC (1:50,13A4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... these cells were stimulated with a 10 μg/ml solution of CD28 and CD49d co-stimulatory antibodies (both from BD Biosciences) and 1ug/ml of 15-mer overlapping peptide pools spanning either the SARS-CoV-2 spike (S) ...
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bioRxiv - Cell Biology 2021Quote: ... This protocol typically produces primary endothelial cell cultures that are approximately 99% pure (determined by immunocytochemistry with an anti-platelet endothelial cellular adhesion molecule 1 [anti-PECAM-1] antibody; BD Bioscience).
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bioRxiv - Immunology 2020Quote: ... For flow cytometry analysis first Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were subsequently washed and stained for following surface markers with directly conjugated antibodies against CD19/B220 (PerCP Cy5.5 or eFluor 450) ...
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bioRxiv - Immunology 2020Quote: ... After subsequent washing step cells were labelled with primary and secondary antibodies or streptavidin-fluorochrome conjugates and were used for flow cytometry analysis (LSR-II, BD Biosciences) or sorting (FACSAria ...
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bioRxiv - Immunology 2020Quote: PBMC were labelled for FACS staining with mouse anti-human antibodies directed against lineage markers (Vioblue-CD3, CD14, CD19, CD56 and CD11c) (BD Biosciences), APC-Vio770 HLA-DR ...
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bioRxiv - Microbiology 2021Quote: ... Purification of human CD34+ cells were assessed by flow cytometry using an anti-human CD34-FITC antibody (clone 581, BD Biosciences). Fetal liver derived from three different donors were used in this study.
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bioRxiv - Biochemistry 2021Quote: ... cells (0.1×106 T cells in 100 μL PBS) were left untreated or were stimulated with anti-CD3 antibodies (145-2C11 (BD Bioscience) or OKT3 (eBioscience ...
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bioRxiv - Cancer Biology 2021Quote: ... Blood samples from the same animals were also stained with the same antibody panel before red blood cell lysis using BD Pharm Lyse lysis buffer (BD Biosciences). All results were acquired using a BD LSRFortessa (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: Spleen and liver cells were incubated (20 min, 4°C) with anti-Fc-gamma blocking antibody (clone 2.4G2) (BD Biosciences, Belgium) and were subsequently stained with fluorescent conjugated antibodies (30 min ...
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bioRxiv - Immunology 2020Quote: ... naïve splenic B cells were enriched by the depletion of CD43 expressing cell using biotinylated anti-CD43 antibody (Clone S7, BD Biosciences), BD IMag Streptavidin Particles Plus and the IMag® system (BD Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated during 30 minutes at 4°C with the following primary antibodies: CD11b (clone M1/70, AF647, #557686, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated with the following primary antibodies during 30 minutes at 4°C: CD11b (clone M1/70, APC-Cy7, #557657, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein (51)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... MLN cells and lung CD11c+ cells were stained for flow cytometry analysis as described above using following fluorophore-conjugated antibodies DAPI (BD Biosciences), CD45 (30-F11 ...
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1 perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2022Quote: ... The membranes were then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk for 2 h ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk in each channel for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunohistochemistry (IHC) was performed on FFEP blocks using mouse monoclonal antibodies SMARCB1/INI1 (Clone 25/BAF47, dilution 1:200, BD Bioscience) and CD70 (Clone 301731 ...
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bioRxiv - Cell Biology 2022Quote: ... cell sorting was employed by labeling intact cells with the fluorescently conjugated HER2 antibody ErbB2 PE-Vio770 and then subjected to fluorescence-activated cell sorting using a FACS Aria III (BD Biosciences), selecting the cells in the top 10% of HER2 expression to generate MDA-MB-231-HER2 stable cells.
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Cardiac fibroblasts regulate cardiomyocyte hypertrophy through dynamic regulation of type I collagenbioRxiv - Cell Biology 2022Quote: ... and the cells were labeled with antibodies as also described above and were then analyzed using a BD FACSCanto cytometer running BD FACSDiVa software (BD Biosciences), using a violet (405 nm ...
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bioRxiv - Genomics 2022Quote: ... This was followed by the secondary labeling step with 1:200 dilution of Streptavidin PE-Cy7 antibody (BD Biosciences, Cat#: 557598). Cells were isolated and sorted using an Influx Cell Sorter Instrument (BD Biosciences ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were stained with fluorochrome-conjugated antibodies (anti-CD15-V450-#642922, anti-CD24-PE-Cy7 #561646, anti-CD44-PE #550989, anti-CD184-PE-#555974; BD Biosciences) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was aspirated and the cell pellet was resuspended in 200uL of FACS buffer (PBS, 0.5% BSA, 2mM EDTA, 20mM Glucose) with 1:50 PE Rat Anti-Human CD49f antibody (BD Biosciences; 555736) and incubated on ice for 20 minutes ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained with fluorochrome-conjugated antibodies or influenza HA-specific (HA518–526) H-2Kd tetramer-IYSTVASSL (MBL) reagent and analyzed using a flow cytometer (BD Biosciences). BMDCs (105 cells ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 and MIP-2 were measured using paired antibodies and recombinant cytokines according to the manufacturer’s recommendations (BD PharMingen Systems) (11).
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bioRxiv - Cell Biology 2019Quote: ... VCAM-1 expression and E-selectin expression were detected by using APC-conjugated monoclonal antibodies anti-CD106 (VCAM-1, clone 51-10C9) and anti-CD62E (E-selectin, clone 68-5H11, BD Biosciences) respectively ...
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bioRxiv - Cancer Biology 2019Quote: DMS-53 cells transduced with DARPP-32 shRNAs or exogenously overexpressed DARPP-32 isoforms were stained with Annexin V-FITC antibodies (BD Biosciences) to assess apoptosis ...
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bioRxiv - Biochemistry 2019Quote: ... After washing with flow cytometry buffer cells were incubated with secondary antibodies for 30 min on ice and wash twice and analyzed by FACScanto instrument (BD Biosciences). Cell fixation and permeabilization was carried out using the Fixation/Permeabilization Solution Kit (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... and then probed overnight at 4°C with the respective primary antibodies diluted in powdered milk block solution at 2.5g/50mL of TBST: mouse anti-CD9 (BD Pharmigen #555370) 1:1000 ...
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bioRxiv - Genetics 2019Quote: ... Cells were then incubated with the following primary antibodies for 1 hour at room temperature: 1:500 mouse IgG2b anti-53BP1 (BD Biosciences) and 1:200 rabbit anti-p21 (eBioscience) ...