Anti CD4 TRX1 citations for Becton, Dickinson and Company

Quote: ... First surface staining was performed using anti-CD4 PerCP/Cyanine 5.5 (clone GK1.5, eBioscience) and anti-CD8a APC (clone 53-6.7, BD pharmingen) for 30 minutes at 4°C ...

T-cell acute lymphoblastic leukemia progression is supported by inflammatory molecules including Hepatocyte Growth factor

Charly Le Maout, et al., bioRxiv - Cancer Biology 2024

Quote: ... stained with anti-CD4 PerCP/Cyanine 5.5 (clone GK1.5, eBioscience) and anti-CD8a APC (clone 53-6.7, BD pharmingen) for 40 minutes at 4°C ...

A recombinant SARS-CoV-2 RBD antigen expressed in insect cells elicits immunogenicity and confirms safety in animal models

Ricardo Choque-Guevara, et al., bioRxiv - Immunology 2021

Quote: ... and then labeled with conjugated antibodies to surface antigens (PerCP-Cy®5.5 anti-mouse CD3, FITC anti-mouse CD4, APC-Cy®7 mouse anti-CD8, all from BD Biosciences ...

Deletion of CD226 in Foxp3⁺ T cells Reduces Diabetes Incidence in Non-Obese Diabetic Mice by Improving Regulatory T Cell Stability and Function

Puchong Thirawatananond, et al., bioRxiv - Immunology 2022

Quote: ... CD4+GFP- Tconv and CD4+GFP+ Treg were sorted on a FACSAria III (BD Biosciences). Sorted cells were then processed with a DNeasy kit (Qiagen) ...

Pharmacokinetics and biodistribution of extracellular vesicles administered intravenously and intranasally to Macaca nemestrina

Tom Driedonks, et al., bioRxiv - Pharmacology and Toxicology 2022

Quote: ... 100 μl whole blood was added to antibody cocktails (mo-anti-CD159a-PE, Beckman Coulter, cat# IM3291U, dil 1:30; mo-anti-CD4-PerCP/Cy5.5 BD Biosciences 552838 dil 1:7.5 ...

Accelerated vaccine induction of IgM neutralising antibody enables vaccine protection against same day lethal influenza virus challenge

Yoshikazu Honda-Okubo, et al., bioRxiv - Microbiology 2021

Quote: ... then stained with APC-anti-mouse CD4 (Clone RM4-5) and PE-anti-mouse CD8a (Clone 53-6.72) (all from BD).

Mice Humanized for Major Histocompatibility Complex and Angiotensin-Converting Enzyme 2 with High Permissiveness to SARS-CoV-2 Omicron Replication

Fabien Le Chevalier, et al., bioRxiv - Microbiology 2022

Quote: ... PE-Cy7-anti-CD4 (clone RM4-5) and BV711-anti-CD8 (clone 53-6.7) mAbs (BD Biosciences or eBioscience). Cells were washed in FACS buffer ...

Exploring the rules of chimeric antigen receptor phenotypic output using combinatorial signaling motif libraries and machine learning

K.G. Daniels, et al., bioRxiv - Synthetic Biology 2022

Quote: ... Antibodies were as follows: PE Mouse anti-human CD4 clone RPA-T4 (BD Biosciences #555347), BV421 Mouse anti-human CD45RA clone HI100 (BD Biosciences #562885) ...

Multivalent, Bispecific αB7-H3-αCD3 Chemically Self-Assembled Nanorings Direct Potent T-cell Responses Against Medulloblastoma

Ellie A. Mews, et al., bioRxiv - Cancer Biology 2022

Quote: ... T cell activation markers were stained with anti-human CD4 (BD Biosciences, clone SK3, BUV395), anti-human CD8 (Biolegend ...

Leaky severe combined immunodeficiency in mice lacking non-homologous end joining factors XLF and MRI

Sergio Castañeda-Zegarra, et al., bioRxiv - Immunology 2020

Quote: The following antibodies were used for flow cytometric analysis: rat anti-CD4-PE-Cy7 (BD Pharmingen™ ...

IL-6 blockade suppresses the blood-brain barrier disorder, leading to prevention of onset of NMOSD

Yukio Takeshita, et al., bioRxiv - Immunology 2021

Quote: ... and rat anti-CD4 antibody (1:100, 550280; BD Pharmingen Inc., San Diego, CA, USA). After overnight incubation with primary antibodies at 4°C ...

Recombinant Fc-fusion vaccine of RBD induced protection against SARS-CoV-2 in non-human primate and mice

Shihui Sun, et al., bioRxiv - Immunology 2020

Quote: ... Then cells were first stained with Percp conjugated anti-monkey CD4 (RM4-5, BD, 561115), Percp conjugated anti-monkey CD8a (RPA-T8 ...

Estradiol Mediates Greater Germinal Center Responses to Influenza Vaccination in Female than Male Mice

Santosh Dhakal, et al., bioRxiv - Immunology 2023

Quote: ... Antibodies used were PerCP-cy5.5 rat anti-mouse CD4 (#55095, clone: RM4-5, BD Biosciences), PE-Cy7 rat anti-mouse CD45R/B220 (#552772 ...

Maresin-1 promotes neuroprotection and prevents disease progression in experimental models of multiple sclerosis through metabolic reprogramming and shaping innate and adaptive disease-associated cell types

Insha Zahoor, et al., bioRxiv - Neuroscience 2024

Quote: ... the cells were stained at the cell surface with FITC-conjugated anti-CD4 (BD Biosciences), APC-conjugated anti-CD25 (BD Bioscience) ...

BATF3-dependent induction of IL-27 in B cells bridges the innate and adaptive stages of the antibody response

Hui Yan, et al., bioRxiv - Immunology 2020

Quote: ... CD19-CD4+CD8- and CD19-CD4+CD8- peripheral blood cells were sorted (BD FACSAria™ II) for genomic DNA extraction and genotyping using PCR involving specific primers ...

IL-33 regulates age-dependency of long-term immune dysfunction induced by sepsis

David F. Colon, et al., bioRxiv - Immunology 2022

Quote: ... CD4 (GK1.5, eBioscience; H129.19, BD Biosciences), CD4 (RPA-T4 ...

Analysis of KSHV B lymphocyte lineage tropism in human tonsil reveals efficient infection of CD138+ plasma cells

Farizeh Aalam, et al., bioRxiv - Immunology 2020

Quote: ... CD4-APC H7 (2μl, BD 560158), CD19-V510 (3μl ...

Identification of human CD4⁺ T cell populations with distinct antitumor activity

Michelle H. Nelson, et al., bioRxiv - Immunology 2020

Quote: ... and APCCy7-CD4 (OKT4, BD Pharmingen). Cells were sorted based on the following gating strategies ...

Phenotypic and Kinetic Changes of Myeloid Lineage Cells in Innate Response to Chikungunya Infection in Cynomolgus Macaques

Brandon J. Beddingfield, et al., bioRxiv - Immunology 2021

Quote: ... CD4-PerCP-Cy5.5 (L200, BD Biosciences), NKG2a-APC (Z199 ...