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Citations for Becton, Dickinson and Company :
3251 - 3300 of 3864 citations for Forkhead Box L1 FOXL1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and panned over prewashed 100-mm tissue culture plates coated with CD45 and CD16/32 antibodies (BD Biosciences, San Jose, CA). After incubation for 60 min at 37°C in a 5% CO2 atmosphere to promote adherence of contaminating macrophages and fibroblasts ...
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bioRxiv - Molecular Biology 2020Quote: ... or anti-Gr-1-PE (cells co-staining with anti-Mac-1 and anti-Gr-1 are deemed to be of the myeloid lineage) monoclonal antibodies (BD Pharmingen). The “percent repopulation” was determined based on the staining results of anti-CD45.2-FITC and anti-CD45.1-PE ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
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bioRxiv - Cell Biology 2022Quote: ... were loaded onto SDS-PAGE gel and immunoblotted using antibodies against clathrin heavy chain (1:1,000; BD Bioscience, San Jose, CA), dynamin (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... After washing, cells were sorted by fluorescence (antibody staining, Kaede-Green and Kaede-Red) on a BD FACS Aria (BD Biosciences) using FACS Diva software and analysis was performed using FlowJo (version 10 ...
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bioRxiv - Immunology 2022Quote: ... Naive CD4+ T cells were stimulated with plate-bound anti-CD3 (LOT: 4310627, eBioscience) and 1.5 μg/ml soluble CD28 monoclonal antibodies (Cat: 557393, BD biosciences pharmingen) in DMEM medium and cytokines (IL-6 ...
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bioRxiv - Immunology 2022Quote: The following mouse anti-human antibodies were used for purification of CD8 T cell subsets by Flow Cytometry: anti-CD45RO PE CF594 (clone UCHL1, BD Biosciences), anti-CD56 PE Cy7 (clone B159 ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were either stained with antibody dissolved in PBS containing 3 % albumin (surface markers) or permeabilized with Perm/Wash buffer (BD Biosciences) according to manufacturer’s instruction and stained with the respective antibody (mix ...
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bioRxiv - Immunology 2021Quote: ... TNFα and CD107a expression by multicolor flow cytometry panel (all antibodies used are listed in Table 1) in a BD LSR Fortessa Instrument (BD Biosciences). Polyfunctionality was evaluated using Boolean gating obtained with FlowJo v10 software.
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with fluorochrome-conjugated (FITC, PE, PE-Cy7, allophycocyanin [APC], APC-eFluor 780,) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with sort buffer and incubated with cell surface antibodies that had been diluted in the BV stain buffer (BD Biosciences) for 30 minutes on ice ...
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bioRxiv - Bioengineering 2019Quote: ... with anti-positive (CD90, CD105, CD44) and negative marker (CD34, CD11b, CD19, CD45, MHC class II) antibodies (Human MSC Analysis Kit, 562245, BD Biosciences), as described in detail in [42] ...
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bioRxiv - Immunology 2020Quote: Cells were stained with indicated fluorophore-conjugated antibodies and samples were collected on either Guava easyCyte HT Flow Cytometer (Luminex) or BD-Accuri C6 Flow Cytometer (BD Biosciences). For intracellular staining of Nur77 ...
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bioRxiv - Bioengineering 2019Quote: ... the cell culture mixture was stained with an antibody recognizing the neural cell adhesion molecule (NCAM/CD56; BD Pharmingen; Table S1), and the myogenic progenitor (CD56+ ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4℃ after 1h blocking in 5% skimmed milk (Becton, Dickinson and Company, USA) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... cover slips were washed 3 times in 1xPBS and incubated with secondary antibodies (anti-rabbit-AF647, anti-mouse-AF488 (BD Bioscience)) diluted 1:1000 in antibody diluent for 1 hour at RT ...
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bioRxiv - Immunology 2019Quote: ... Cells were further incubated for an additional 30 minutes on ice with a mixture of the following antibodies: Alexa Fluor 700 rat anti-mouse CD45R (B220, clone RA3-6B2, BD Biosciences), FITC rat anti-mouse CD43 (Clone S7 ...
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bioRxiv - Molecular Biology 2019Quote: ... The HLA-DQ cell surface expression was performed by cytofluorimetric analysis using the FACSAria III and DIVA software with FITC mouse anti-human HLA-DQ antibody (BD Biosciences). The quantitation of specific transcripts was performed by qRT-PCR as described in [9] ...
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bioRxiv - Immunology 2019Quote: ... biotinylated anti-rat CD161 (Clone 10/78) and biotinylated anti-rat granulocyte marker (Clone HIS48) antibodies (BD Biosciences, San Jose, CA) followed by magnetic separation (Miltenyi Biotec ...
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bioRxiv - Pathology 2019Quote: ... were added in triplicate for each experimental group to the 96-well plates pre-coated with 3 μg/ml anti-CD3 antibody (clone HIT3a, BD Biosciences) for 3-5 h at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were fixed in 4% paraformaldehyde for 30 minutes and stained with PE-conjugated or purified CD45 antibody (eBioscience, 30-F11 or BD Biosciences) to ascertain the generation of haematopoietic progeny ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Genomics 2021Quote: ... tissues were blocked (3% bovine serum albumin in 5% normal goat sera and 0.1% Triton) and incubated overnight at 4°C with primary antibodies: CD31 (Neomarkers, RB-10333-P0) or CDH1 (BD Biosciences, 6315829). Slides were washed and incubated with Cy3-goat-anti-mouse or anti-rabbit-conjugated secondary antibodies (Jackson Immunoresearch Laboratories ...
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bioRxiv - Immunology 2021Quote: Post-culture cells were pelleted in V-bottom 96-well plates and resuspended in 30 μl of antibodies at pre-optimized concentrations and diluted in Brilliant Stain Buffer (BD Biosciences), then incubated for 15’ at RT ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with a panel of antibodies (Supplementary Table 1) in flow cytometry staining buffer with BD Horizon Brilliant Stain Buffer Plus (BD Biosciences) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... one section out of ten was incubated with BrdU antibodies (BrdU, 1/1,000, CldU, 1/500, Accurate Chemical; IdU, 1/500, BD Bioscience). Bound antibodies were visualized with Cy3-goat anti-rat antibodies (1/1,000 ...
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bioRxiv - Microbiology 2020Quote: ... for 5 min and incubated for 30 min with optimal concentrations of fluorochrome-conjugated antibodies (Table S2) diluted in Brilliant Stain Buffer (BD Biosciences). After three washes with FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 hr at 4°C with extracellular domain anti-GluN1 antibody (BD biosciences; 2 μg ml−1) followed by two washes with ice cold phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with fluorochrome-conjugated (BV421, BV711, FITC, PE, PE-Cy7, allophycocyanin [APC], APC-Cy7) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were then incubated with primary antibodies (ACE2, ab15438, Abcam, UK; HCG beta, ab9582, Abcam, UK; HLA-G, 557577, BD Pharmingen) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells or MNC from CML patients or HD were incubated with the following antibodies: 5 µL CD45-PerCP (2D1, BD Biosciences), 2.5 µL CD34-FITC (AC136 ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Bioengineering 2021Quote: ... at 1:1 E:T ratio at 37°C with 5% CO2 in the presence of a CD107a antibody for 1 hr before treatment with brefeldin A (10 µg/ml; BD Biosciences) and monensin (0.7µg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... An antibody panel for macrophage and T cell polarization was also stained for consisting of: F4/80 BUV395 (T45-2342, BD Biosciences), CD86 BV786 (GL-1 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 72 h at 4°C in mouse anti-PKCδ primary antibody (1:1000, BD Biosciences, 610397) in blocking solution containing 1.5% NGS ...
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies detecting Active Caspase-3 (C92-605) and BCL2L11 (Y36) was performed using BD Cytofix/Cytoperm (BD Biosciences) or eBioscience FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Immunology 2021Quote: ... and stained for 30 min on ice with a cocktail of antibodies diluted in a 1:1 mixture of FACS buffer and the Brilliant Stain Buffer (BD Biosciences). The antibody cocktail consisted of APC/Cy7-CD3 (SK7 ...
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bioRxiv - Neuroscience 2020Quote: ... Mononuclear cells from the remaining five brains were labeled with APC-Cy7 rat anti-CD11b antibody (BD Pharmingen, Cat. No. 557657) for 30min at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were re-stimulated for another 6 h with the anti-CD3 antibody in the presence of both GolgiStop and GolgiPlug (BD Biosciences) and stained with antibodies against IFN-γ and TNF-α ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with antibodies according to the manufacturer’s specifications and analyzed with a BD FACSCelesta™ Flow Cytometer (BD Biosciences). Engraftment and lineage analysis was determined by staining peripheral blood with CD45.1 (BioLegend Cat#110708 ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...
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bioRxiv - Cell Biology 2020Quote: ... intracellular staining with anti-FoxP3-FITC antibodies (clone: FJK-16s; Thermo Fischer Scientific) was performed with the BD Cytofix/Cytoperm Kit (BD Biosciences) according to the manufacture instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The levels of DNA-loaded MCM were detected by incubating cells in anti-MCM2 BM28 antibody (1:200, BD biosciences, 610700) for 1 hr at 37°C in the dark followed by incubation in anti-mouse-488 secondary antibody (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were activated in plates coated with anti-mouse CD3ε and anti-mouse CD28 antibodies (1 µg/ml, BD Biosciences) for up to 72h.
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bioRxiv - Cell Biology 2022Quote: ... 100 ug of clarified lysates were diluted to a final concentration of 2 ug/ul and incubated with 60 ul of Dynabeads prebound to 7.5 ug of either anti-Cdk1 Antibody (BD Biosciences - 610037) or Mouse Ig-G ...