2 1 ethylpentyl 1 3 dioxan 5 yl laurate citations for Becton, Dickinson and Company

Quote: ... 5 µg of LPP formulated circRNA or linear RNA encoding Firefly Luciferase (FLuc) in PBS were administrated into mice (n=3 for each group) intramuscularly with 3/10 insulin syringes (BD biosciences). Mice were then anesthetized for whole-body bioluminescence imaging with an IVIS Spectrum (Roper Scientific ...

Combination EZH2 inhibition and retinoic acid treatment promotes differentiation and apoptosis in rhabdomyosarcoma cells

Eleanor O’Brien, et al., bioRxiv - Cancer Biology 2023

Quote: Apoptosis was measured by evaluating the activation of caspase 3/7 using the Ac-DEVD-AMC Caspase-3 Fluorogenic Substrate (BD Biosciences) according to manufacturers’ instructions ...

Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques

Timothy N. Hoang, et al., bioRxiv - Immunology 2020

Quote: ... anti-CD3 BV605 (clone SP34-2; 2 μL; cat# 562994) from BD Biosciences ...

Prime-boost vaccination of mice and Rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates

Shengxue Luo, et al., bioRxiv - Immunology 2020

Quote: ... interleukin-2 (IL-2) and tumor necrosis factor α (TNF-α) (BD). All samples were tested with BD FACS Canton flow cytometer (BD).

Actin isovariant ACT7 controls root meristem development in Arabidopsis through modulating auxin and ethylene responses

Takahiro Numata, et al., bioRxiv - Plant Biology 2022

Quote: ... 9 cm Petri plates on modified Hoagland’ s medium (Baskin and Wilson, 1997) containing 1% w/v sucrose and 1% w/v agar (Difco Bacto ager, BD laboratories; http://www.bd.com). Two days after stratification at 4°C in the dark ...

Presynaptically silent synapses are modulated by the density of surrounding astrocytes

Kohei Oyabu, et al., bioRxiv - Neuroscience 2020

Quote: ... and then were stamped with a 1:1 mixture of rattail collagen (final concentration 1.0 mg/mL, BD Biosciences, San Jose, CA, USA) and poly-D-lysine (final concentration 0.25 mg/mL ...

Dual RNAseq highlights the kinetics of skin microbiome and fish host responsiveness to bacterial infection

J. Le Luyer, et al., bioRxiv - Genomics 2020

Quote: ... TFA4 strain was cultivated in nutrient Zobell medium (4 g L−1 peptone and 1g L−1 yeast extract Becton, Dickinson and Company, Sparks ...

Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfate

Endika Prieto-Fernández, et al., bioRxiv - Cell Biology 2021

Quote: ... washed with blocking buffer and incubated with fluorochrome-conjugated antibodies (1:100 dilution in blocking buffer) against syndecan-1 (BD Biosciences Cat#: 565943), syndecan-2 (R&D Systems Cat# ...

DYNLL1 mis-splicing is associated with replicative genome instability in SF3B1 mutant cells

Annie S. Tam, et al., bioRxiv - Cell Biology 2021

Quote: ... IdU and CldU were then detected with the following primary antibodies in blocking solution for 1 hour at room temperature: mouse anti-BrdU (B44) (1:25) (BD Biosciences cat#347580) for IdU and rat anti-BrdU [Bu1/75 (ICR1)] (1:25 ...

Epithelial-mesenchymal plasticity through loss of CTCF motif accessibility and protein expression

Kelsey S. Johnson, et al., bioRxiv - Cell Biology 2021

Quote: ... cells were counted and resuspended in 500 µL 1% FBS (Cytiva) in PBS with anti-E-cadherin BB700 (BD Biosciences #745965, 1:100), anti-CD44 BV421 (BioLegend #562890 ...

Azole resistance is mediated by integration of sterol gene regulation and membrane transporter production by the zinc cluster-containing transcription factor Upc2A in Candida glabrata

Bao Gia Vu, et al., bioRxiv - Genetics 2021

Quote: ... and the mixed culture [1:1 ratio] was grown either aerobically [normoxic] or anaerobically [in a GasPak chamber (BD Biosciences, San Jose, CA)] for 24 hours ...

A spike-ferritin nanoparticle vaccine induces robust innate immune activity and drives polyfunctional SARS-CoV-2-specific T cells

Joshua M. Carmen, et al., bioRxiv - Immunology 2021

Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin, 1 µg/mL, BD Biosciences) was added followed by an additional 5-hour incubation at 37°C ...

A spike-ferritin nanoparticle vaccine induces robust innate immune activity and drives polyfunctional SARS-CoV-2-specific T cells

Joshua M. Carmen, et al., bioRxiv - Immunology 2021

Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin, 1 µg/mL, BD Biosciences) was added for an additional 5 hours at 37°C ...

Caveolin-1 temporal modulation enhances trastuzumab and trastuzumab-drug conjugate efficacy in heterogeneous gastric cancer

Patrícia M. R. Pereira, et al., bioRxiv - Cancer Biology 2021

Quote: ... A total of 5 million cells were suspended in 150 μL of a 1:1 v/v mixture of medium with reconstituted basement membrane (BD Matrigel, BD Biosciences) and injected subcutaneously in each mouse.

Cell-intrinsic ceramides determine T cell function during melanoma progression

Matthias Hose, et al., bioRxiv - Immunology 2022

Quote: ... T cells were stimulated with 1 μg/ml anti-CD3 plate-bound and 1 μg/ml anti-CD28 soluble (both BD Biosciences, Heidelberg, Germany) in IMDM complete culture medium ...

Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human platelets

Anastasiia Stratiievska, et al., bioRxiv - Cell Biology 2023

Quote: ... samples were stained with primary anti-TRPM8 (1:100; #ACC-049, Alomone) and CD41a PerCP-Cy™5.5 (1:100, #340930, BD, Franklin Lakes, NJ) antibodies in PBS with 10% normal goat serum for 1hr in the dark and a Hoechst nuclear stain at 1 ug/ml ...

Fgf signalling is required for gill slit formation in the skate, Leucoraja erinacea

Jenaid M. Rees, et al., bioRxiv - Developmental Biology 2023

Quote: ... and then blocked in 1% bovine serum albumin in PBST before addition of primary antibodies against E-cadherin (1:200, BD Transduction Laboratories, 610181), laminin (1:200 ...

In Vivo Biorthogonal Antibody Click for Dual Targeting and Augmented Efficacy in Cancer Treatment

Sandeep Surendra Panikar, et al., bioRxiv - Cancer Biology 2023

Quote: ... and/or 0.25 million A431 cells in a 100 μL cell suspension of a 1:1 (v/v) mixture of medium with reconstituted basement membrane (BD Matrigel, BD Biosciences). Mice-bearing NCIN87/A431 bilateral tumors were developed by subcutaneous injection of NCIN87 and A431 cancer cells on the bilateral dorsal flank regions ...

Traitor-virus-guided discovery of novel antiviral factors

Caterina Prelli Bozzo, et al., bioRxiv - Microbiology 2023

Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...

Direct PA-binding by Chm7 is required for nuclear envelope surveillance at herniations

David J. Thaller, et al., bioRxiv - Cell Biology 2020

Quote: ... 2% Bacto-peptone (BD), 0.025% adenine hemi-sulfate (Sigma) ...

Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants

Liang Qu, et al., bioRxiv - Immunology 2022

Quote: ... 557998)/IL-2 (BD, 560547)/IL-4 (BD ...

Type I IFN promotes pathogenic inflammatory monocyte maturation during H5N1 infection

Slim Fourati, et al., bioRxiv - Immunology 2022

Quote: ... 2% Fc block (BD), and fixable viability dye eFluor 450 (ebiosciences) ...

Protease activities of vaginal Porphyromonas species disrupt coagulation and extracellular matrix in the cervicovaginal niche

Karen V. Lithgow, et al., bioRxiv - Microbiology 2021

Quote: ... with 2% gelatin (BD), 1% yeast extract (ThermoFisher Scientific ...