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Citations for Becton, Dickinson and Company :
3001 - 3050 of 8007 citations for 6 methyl N1 4 pyridin 3 yl thiazol 2 yl benzene 1 3 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... PANC-1 cells (1 × 107) were implanted with Matrigel (BD Biosciences, San Jose, CA, USA) into the pancreas of each mouse.
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bioRxiv - Immunology 2024Quote: ... 1% Penicillin/Streptomycin and Protein Transport Inhibitor (Containing Brefeldin A) (diluted 1:1000) (BD, 555029). The cells were incubated for four hours at 37°C and further washed with 1x PBS to proceed for intracellular cytokine staining.
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bioRxiv - Neuroscience 2020Quote: ... on alternate one-in-ten sections using different anti-BrdU antibodies from different vendors (for BrdU: 1/200, Dako, Glostrup, Denmark; CldU: 1/500, Accurate Chemical, Westbury, USA; IdU: 1/200, BD Biosciences, San Jose, USA) and Zif268 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were fixed in 2% PFA and subsequently acquired using a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA). Flow data were analyzed using FlowJo v10 software (Tree Star ...
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bioRxiv - Immunology 2021Quote: ... cells were either resuspended in 400μl of PBS 2%FCS 2mM EDTA and analyzed on Fortessa-X20/Symphony cytometers (BD Biosciences) or in RPMI media and used for cell sorting on a FACSAria II (BD ...
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bioRxiv - Immunology 2019Quote: ... whole blood was stained within 2 hours of blood draw for the surface markers CD45 (PerCP; DO58-1283; BD Biosciences), CD3 (FITC ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde and were examined in a Canto II flow cytometer (BD Bioscience, San Jose, CA), following a previously reported protocol [49] ...
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bioRxiv - Immunology 2021Quote: ... at 100ng/mL and IL-2 (100IU/mL) or expanded by plate-bound anti-CD3 mAb at 2μg/mL (BD Pharmingen) and soluble anti-CD28 mAb at 1μg/mL (BD Pharmingen ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in T cell media supplemented with soluble anti-mouse CD28 (2 μg/ml, Clone: 37.51, BD Pharmingen) and 30 U/ml rhIL-2 (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... The concentrations of cytokines IL-2 and IFN-γ were quantified using the corresponding ELISA kits (BD, 555190 and 555142).
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bioRxiv - Immunology 2020Quote: ... 2 x 106 cells were cultured in 200μL final volume in 5mL polypropylene tubes (BD Biosciences, San Diego, CA, U.S.A) in the presence of anti-CD28 (1μg/mL ...
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bioRxiv - Microbiology 2020Quote: 100 μl of washed and number-adjusted yeast suspension were injected into each caterpillar’s distal right proleg with a 30G1/2” needle (BD Microlance) and a 1 ml NORM-JECT syringe ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were incubated on a cold block for 2-5 minutes before being plated in a 96 (12 x 8) grid on Omnitrays containing LB (BD, Franklin Lakes ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then washed with FC buffer (PBS supplemented with 2% HI-FBS and 0.05% NaN3) and resuspended in Fixation Buffer (BD Pharmingen)for 5 min at 4C° in the dark ...
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bioRxiv - Developmental Biology 2022Quote: ... E.coli strain Rosetta™ 2(DE3)pLysS bearing pET21d-FGF10 was grown in 2x YT medium (BD Biosciences, Cat#244020) with Carbenicillin (50µg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Collagenase activity was quenched by dilution with FACS buffer (PBS, 0.5% FBS, 2 mM EDTA) and digestion products were filtered through 70 µm nylon filters (BD Biosciences). Cells were again washed with FACS buffer and immediately processed for immunostaining first by blocking Fc receptor binding with rat anti-mouse CD16/CD32 (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... Flow cytometry profiles of the HSPC compartment (Extended Data Fig.2, Fig.9) were analysed using FlowJo software (version 10.1, BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 2 ml of FACS buffer and fixed by adding 3.5 ml of fixation/permeabilization buffer (BD Biosciences) and rocking overnight at 4C ...
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bioRxiv - Pathology 2021Quote: ... Whole blood samples were then lysed and fixed in 2 ml of FACS Lysing Solution (BD Biosciences, San Jose, CA) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were first blocked for non-specific antigen binding (incubating for 20 min at RT with 2 µg/ml CD16/CD32 Rat anti-Mouse, unlabeled, Clone: 2.4G2, BD, 553142) then antibodies were added without washing off the blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained with antibody (Supplementary Table. 2) and LSK cells were isolated through cell sorting using FACSAria (BD). Sorted LSK cells were cultured in StemSpan SFEM serum free media (stemcell technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Pre-coated coverslips were printed by stamping (0.2 mm spot diameter and 0.5 mm spot interspace) with a cell-attachment mixture of rat tail collagen (BD Biosciences), poly-D-lysine (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Biochemistry 2022Quote: ... washed and resuspended in FACS buffer (2% FBS in 1X PBS) and filtered through filter-topped flow tubes (BD falcon) using a luer-lock syringe at 2×106 cells/ml ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cells were resuspended in PBS supplemented with 2% FBS and then analyzed with BD FACSCalibur™ (BD, Bedford, MA, USA). Raw data were analyzed by FlowJo software.
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bioRxiv - Biochemistry 2019Quote: ... shRNA expression was induced by doxycycline (2 μg/ml) and sorted using a BD FACSAria™ III (BD Biosciences-US) cell sorter to select for the high TurboRFP expressing cells ...
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bioRxiv - Bioengineering 2019Quote: Blood was gently drawn from healthy volunteers who had not been subjected to any anticoagulant treatments for at least 2 weeks by standard venepuncture into sodium citrate vacutainers (2.7 mL, 3.2 % v/v sodium citrate; BD, NJ, USA). The informed consent was provided by each volunteer ...
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bioRxiv - Immunology 2019Quote: ... and activated with 2 μg/mL anti-mouse RP105 clone RP/14 (produced in house or BD Pharmingen Cat.# 562191).
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bioRxiv - Microbiology 2020Quote: ... cells were resuspended and 2-43 binding to cell-surface S trimer was quantified on LSRII flow cytometer (BD Biosciences). The mean fluorescence intensity of APC in GFP-positive cells was analyzed using FlowJo and the relative binding of 2-43 to S trimer in the presence of competitors was calculated as the percentage of the mean fluorescence intensity compared to that of the competitor-free controls.
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer (PBS with 2% Heat-inactivated FCS) and fixed in 1x BD cellFIX (BD Biosciences). Samples were acquired on the LSRFortessa (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were washed with 2 ml wash buffer and taken up in 0.5 ml wash buffer afterwards for FACS analysis (BD Fortessa).
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bioRxiv - Immunology 2020Quote: ... the cells washed and stained with CD45.2-FITC (to identify leukocytes) and cell subset specific fluorochrome-conjugated mAb used in this study were obtained from BD Biosciences and Biolegend ...
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bioRxiv - Immunology 2020Quote: OT-I cells were expanded for 5 days in the presence of IL-2 after transduction and sorted on Thy-1.1 surface expression on a BD FACSAria Fusion cell sorter (BD Biosciences) directly into RLT Plus lysis buffer (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were pelleted and then resuspended in 2 mL of 0.5% BSA in D-PBS for cytometer-based sorting (FACSAria IIIu cell sorter, BD Biosciences). An aliquot of 200 µL stained cells was saved as input for analysis ...
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bioRxiv - Immunology 2021Quote: ... followed by FcR blockage with mouse serum (2%) and FcR blocking anti-mouse CD16/32 antibodies (clone 2.4G2, BD Biosciences). Next ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed twice in PBS with 0.5% BSA and 2 mM EDTA and run on LSR II flow cytometer (BD Biosciences). Data were analyzed using FlowJo software ...
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bioRxiv - Immunology 2021Quote: Cells resuspended in cold staining buffer (2% FCS in PBS) were blocked with mouse Fc Block (BD Biosciences, catalogue# 553141) for 10 minutes at 4°C and stained with a panel of antibodies (Supplementary Table 5) ...
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bioRxiv - Immunology 2021Quote: ... tibias and pelvis were harvested by crushing in PBS containing 2% of FBS and erythrocytes were lysed using a hypotonic buffer from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and IL-2 concentration were measured on non-diluted or 10-fold diluted supernatants using Human cytometric assay from BD according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... the invading cancer cells were fixed with 100% cold methanol and stained with 0.05 mg/mL 4,6-diamidino-2-phenylindole (DAPI; BD Biosciences, 564907). The invading cells in each chamber were counted under a fluorescence microscope ...
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bioRxiv - Cancer Biology 2020Quote: ... single cell suspensions were made (1.5 x 104 cells per ml) in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 150μl of this mix was plated per well in a 96 well ImageLock plate (Essen Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and the concentration of IL-2 in culture supernatants was determined by ELISA according to the manufacturer’s instructions (BD Biosciences).
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bioRxiv - Genetics 2021Quote: ... in 200 μl of 2% FBS/PBS (v:v) and calculated using DIVA before sorting on a FACSAria fusion III cytometer (BD Biosciences). In order to maintain the ratios of MuSCs ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5mM EDTA) containing the indicated fluorophor-conjugated antibodies (CD138-APC [281-2] and B220-PE [RA3-6B2] purchased from BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... and stained for membrane extracellular markers in Staining Buffer (PBS FBS 2% EDTA 5mM) and for intracellular markers using Cytofix/Cytoperm buffer set following manufacturer’s protocol (BD Biosciences). Acquisition of cytometric parameters was performed on an LSRII (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: One hundred microliters of whole blood from each of the cynomolgus macaques was stained with combinations of fluorescence-conjugated monoclonal antibodies: anti-CD3 (clone SP34-2, Alexa700; BD), anti-CD4 (clone L200 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by fixation in 2% PFA in PBS before subjecting them to flow cytometry using the LSR-II (BD Biosciences). Data was analyzed by FlowJo v10 and graphed by Prism 8 (GraphPad).
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bioRxiv - Immunology 2020Quote: ... USA) for 2 hrs followed by the addition of Brefeldin A (10 µg/ml) (BD Biosciences, San Diego, CA, USA) after which the cells were further incubated for 16 hrs ...
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bioRxiv - Immunology 2020Quote: ... T cells were 2-way sorted from PBMC pre-gated on CD4+ CD14− viable lymphocytes using a FACS Aria (BD) and the antibodies listed in supplementary material procured from Biolegend or BD ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed 2 times with flow buffer and incubated with BD Cytoperm™ Permeabilization Buffer Plus (BD Biosciences) for 10 minutes at 4°C ...