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Citations for Becton, Dickinson and Company :
251 - 300 of 331 citations for mmu mir 212 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... for 5 min at RT prior to add a 50 μl droplet of collagen type I (BD bioscience - 50:1 ratio unlabeled vs Alexa548-labeled ...
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bioRxiv - Immunology 2024Quote: ... samples were fixed/permeabilized for 20 min at RT with the BD Cytofix/Cytoperm kit (BD Biosciences) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Intracellular cytokine staining was performed according to the manufacturer’s protocol (Cytofix/Cytoperm buffer set from BD with PE-Cy7-conjugated anti-CD4 (RM4-5), eFluor 450-conjugated anti-TCR-β (H57-597) ...
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bioRxiv - Neuroscience 2021Quote: ... using BD CBA Flex Sets (GCSF: #560152, KC: #558340, IL-1α: #560157, IL-1β: #560232, IL-6: #558301, TNF-α: #558299, BD, Becton, Dickinson and Company) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Genomics 2021Quote: ... FCM were performed on 50 μl sample in triplicate at a pre-set flow rate of 66 μl.min-1 using a BD Accuri® C6 flow cytometer (BD Accuri® cytometers, Belgium) which is equipped with a 50mW solid state laser emitting light at a fixed wavelength of 488 nm ...
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bioRxiv - Immunology 2022Quote: The levels of cytokines in cell culture medium were determined with BD OptEIA™ Mouse IL-6 ELISA set (BD Biosciences, Franklin Lakes, NJ), ELISA MAX™ Standard Set Mouse IL-10 (BioLegend) ...
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bioRxiv - Cell Biology 2022Quote: ... Staining was performed for 20min at room temperature (RT) using CD3ε (Lin) – APC clone 145-2C11 (553066, BD), TER-119 (Lin ...
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bioRxiv - Neuroscience 2021Quote: ... slides were counterstained with DAPI (1:250 in 1X PBS, 10 mins at RT, Cat: 564907, BD Pharmingen) and mounted with Prolong™ Glass anti- fade mounting medium (Cat ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... All blood samples were clotted for 30-60 min at RT in serum separator tubes (BD, Cat# 365967), and sera were isolated by centrifugation at 4,000 x g for 15 min at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by overnight incubation at RT with combinations of primary antibodies including anti-CD31 (1:300, BD pharmingen), -HMGB1 (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by secondary antibodies for 30 min in RT in Perm/Wash buffer (BD Biosciences, catalog no. 554714) supplemented with 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... Serum was collected by tail-tipping before LPS injection and 2 and 4 hours following injection for IL6 determination by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufactures instructions.
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bioRxiv - Immunology 2021Quote: All flow cytometry assays were set to acquire 10,000 events excluding debris and doublets using a FACSVerse™ flow cytometer (BD Biosciences, San Diego, CA, USA) and FlowJo v10.6 software (Ashland ...
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bioRxiv - Neuroscience 2021Quote: ... using BD CBA Flex Sets (GCSF: #560152, KC: #558340, IL-1α: #560157, IL-1β: #560232, IL-6: #558301, TNF-α: #558299, BD, Becton, Dickinson and Company) according to the manufacturer’s instructions ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... ELISA was performed to measure IL-2 concentration of the thawed samples by using mouse IL-2 ELISA set (BD Biosciences, Franklin Lakes, NJ, USA) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... IgA and IgM antibodies were quantified in the CVL specimens using the human immunoglobulin cytokine bead array (CBA) flex set assay (BD Biosciences, San Jose, CA, USA). The Ig CBA assay was performed as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and tumour necrosis factor α concentrations were quantified in lung homogenates using an ELISA kit (BD Opt EIA Mouse IL-6 ELISA Sets, #555240; BD Biosciences Franklin Lakes, NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were purified with AMPure Beads (BD Bioscience ...
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bioRxiv - Immunology 2021Quote: ... and stained with antibodies directly conjugated to fluorochromes for 1hr at RT in 1X Perm/Wash buffer (BD Biosciences). NK cells were gated as viable ...
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bioRxiv - Microbiology 2022Quote: ... RT) and resuspended in 2 ml of PBS and immediately analyzed by flow cytometry (BD FACS Canto II Instrument). For each sample ...
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bioRxiv - Bioengineering 2023Quote: ... fixed with 4% PFA for 20 minutes at RT and permeabilized using Cytofix/Cytoperm (BD Biosciences, catalog no. 554714) for 20 min on ice followed by twice washes with 1x Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... OncoImmunin Inc.) was added (5 min. at RT) and cells were acquired with a FACScan flow cytometer (BD BioSciences). The percentage of double positive eFluor+ and granzyme B+ target cells was calculated.
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bioRxiv - Developmental Biology 2023Quote: ... then incubated overnight at room temperature (RT) with combinations of primary antibodies including anti-CD31 (1:200, BD pharmingen), anti-GFP (1:500 ...
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bioRxiv - Cancer Biology 2019Quote: ... Gates were set to sort cell populations having low 10% and high 10% expression of ITGB4 using the FACSAria™ Fusion (BD Biosciences, San Jose, CA, USA). Sorted cells were immediately cultured in 12-well plates and treated with cisplatin (1 μM ...
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bioRxiv - Immunology 2021Quote: ... and anti-inflammatory cytokines (IL-10, TGF-β) were determined in plasma by ELISA at the indicated time points (OptEIA set; BD Biosciences, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were blocked and incubated with the following primary antibodies for 1 hour at RT in humidity chamber (anti-CldU, Abcam ab6326, 1:100; anti-IdU, BD 347580 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated for 1 h at RT with primary antibody used as follows: mouse anti-EEA1 (BD Biosciences: 610457) 1 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... at 3:100 or phospho-AKT (pS473) at 1:20 dilutions for 30 mins at RT before analysis on FACSAria II (BD).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1% TritonX100 in PBS for 20 min at RT and stained overnight with rat-anti-PECAM1/CD31 antibodies (1/100, clone Mec13.3, BD Pharmingen) in blocking solution and secondary Alexa594 goat anti-rat IgG antibodies and DAPI (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340, BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by blocking with 3 % normal goat serum (NGS) in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were first blocked for non-specific antigen binding (incubating for 20 min at RT with 2 µg/ml CD16/CD32 Rat anti-Mouse, unlabeled, Clone: 2.4G2, BD, 553142) then antibodies were added without washing off the blocking solution ...
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bioRxiv - Immunology 2020Quote: ... for 5 min at RT prior to staining with a surface stain cocktail containing the following antibodies purchased from BD and Biolegend ...
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bioRxiv - Evolutionary Biology 2022Quote: The cryosections were blocked with 1% skim milk in PBS with 0.5% Tween-20 at RT for 15 min and incubated with primary antibody (rat anti-mouse anti-PECAM-1 [CD31; BD Pharmingen ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards, they were washed twice by centrifuging (500 g, 5min, RT) and resuspending in Stain Buffer (#554656, BD Pharmingen, USA). Cells were fixed in Fixation Buffer (#554655 ...
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bioRxiv - Cell Biology 2023Quote: ... pH 6.5) and specifically stained for 15 min at RT using an antibody against GPVI (HY101-PE, #565241, BD PharmingenTM) at a ratio of 1:10 ...
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bioRxiv - Plant Biology 2021Quote: ... The AtCGL160 CDS without the signal peptide (see Supplemental Table S2 for primer information) was cloned into the bait vector pGBKT7 (BD-AtCGL160), whereas the coding sequences of CF1-α ...
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bioRxiv - Immunology 2019Quote: ... cDNA was amplified - 10 cycles for resting cells and 9 cycles for in vitro activated cells - using the Human Immune Response primer panel (BD Biosciences), containing 399 primer pairs ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured by a SPOT RT monochrome charge-coupled device camera (Diagnostic Instruments, Sterling Heights, MI) and processed by IPLab imaging software (BD Biosciences) and Photoshop CS3 (Adobe ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured by a SPOT RT monochrome charge-coupled device camera (Diagnostic Instruments, Sterling Heights, MI) and processed by IPLab imaging software (BD Biosciences) and Photoshop CS3 (Adobe ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...
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bioRxiv - Immunology 2021Quote: ... Single cells were index sorted into 384 well plates containing 1uL of reverse transcription (RT) master mix using a FACS Aria III with FACS Diva software (BD Biosciences) to maintain protein expression with plate well location ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were stained in a solution containing 1 μg/ml PI and 4 μg/ml RNase A in PBS at 2 x 106 cells/ml for a minimum of 30 min at RT Cell cycle analysis was performed on a LSR Fortessa analyser (BD Biosciences) and analysed using FlowJo software.
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bioRxiv - Immunology 2022Quote: ... fixable viability dye was included with the surface staining antibodies (20 minutes, RT) and intracellular staining used the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... After washing in PBS and incubated for 1 hour at RT with secondary donkey-anti-rat rhodamine red 1:100 (both from BD Pharmingen) in antibody buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C and secondary antibodies for 30 min in RT in Perm/Wash buffer (554714, BD Biosciences) containing 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... the following antibodies and viability dye were incubated with PBMCs at RT for 20 min: anti-CD3 BUV395 (clone SP34-2; BD Biosciences), anti-CD4 BUV495 (clone SK3 ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The sections were then blocked and permeabilized with 5% Donkey serum 0.01% Triton X-100 for 30 min at RT before being incubated with rabbit anti-YAP dilution 1/100 (Signalling Technology #14074) and mouse anti beta-catenin (BD 610153) (dilution 1/200 ...
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bioRxiv - Immunology 2021Quote: ... Single cells were index sorted into 384 well plates containing 1uL of reverse transcription (RT) master mix using a FACS Aria III with FACS Diva software (BD Biosciences) to maintain protein expression with plate well location ...