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Citations for Becton, Dickinson and Company :
2901 - 2950 of 3149 citations for Recombinant Human ROR1 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Surface marker proteins and intracellular cytokine responses were quantified via flow cytometry (LSRII, BD Biosciences) and analyzed using FlowJo software version 10.5.3 (TreeStar Inc. ...
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bioRxiv - Immunology 2021Quote: ... Cell surface expression of adhesion molecule protein was measured by multiparameter flow cytometry (BD Fortessa). Supernatants were retained and secreted chemokines were measured by ELISA (IP-10 ...
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bioRxiv - Immunology 2020Quote: ... Expression of proteins was measured using FACS Canto II HTS (BD Biosciences, Bedford, MA, USA) and analyzed using FlowJo software (FlowJo LLC ...
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bioRxiv - Immunology 2020Quote: ... HA proteins were tetramerized through sequential addition of streptavidin-BV650 or streptavidin-BV785 (BD biosciences), with HA in excess to streptavidin ...
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bioRxiv - Microbiology 2021Quote: ... Expression levels of surface S proteins were analyzed using a FACS Canto II (BD Biosciences). RL activity was normalized to the mean fluorescence intensity (MFI ...
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bioRxiv - Microbiology 2019Quote: ... 5% Skim Milk Protein at 1/2x Tryptic Soy Broth (Becton, Dickinson and company, France) medium and 100 μL of F ...
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bioRxiv - Plant Biology 2021Quote: WRKY proteins used for this experiment were fused into pGBT9 vector (containing GAL4 DNA-BD) and transformed into yeast strain Y190 ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained with antibodies specific for secretory proteins: Granzyme B (BD, Cat. # 396407), Interferon-gamma (BD ...
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bioRxiv - Immunology 2023Quote: ... and 1 µL/mL GolgiPlug™ Protein Transport Inhibitor (containing Brefeldin A, BD Biosciences, #555029). Cell suspensions were washed between each step with sterile PBS containing 2% FBS (FACS buffer).
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bioRxiv - Physiology 2023Quote: Protein concentrations of multiple serum cytokines were measured using a CBA Kit (BD Biosciences: 558264) and flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... for 4 h and with (1:1500 dilution) GolgiStop protein transport inhibitor (#554724, BD Biosciences) added for the last 2 h of stimulation prior surface staining ...
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bioRxiv - Immunology 2024Quote: ... for 4-16 hours at 37C with the protein-transporter inhibitor Golgi Stop (BD Biosciences) added during the last 4 hours of culture ...
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bioRxiv - Immunology 2024Quote: ... 1% Penicillin/Streptomycin and Protein Transport Inhibitor (Containing Brefeldin A) (diluted 1:1000) (BD, 555029). The cells were incubated for four hours at 37°C and further washed with 1x PBS to proceed for intracellular cytokine staining.
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 50 µL of FACS buffer (PBS plus 1% BSA) and blocked with µL 1 human Fc block (BD Biosciences, 564219) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Flow cytometry was conducted and the supernatant was collected from each well to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... Then cells were blocked by PBS plus 5% BSA and later incubated with FITC Anti-Human CD24 (BD Biosciences, San Jose, CA), and PE Anti-Human CD44 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... To assess activation marker status T cells were stimulated with 0.1 μg/mL of D117C or PIGF on collagen coated plates for 16-20 hr and surface-stained with anti-human CD3 BUV395 (BD clone SK7), CD4 BV785 (Biolegend clone RPA-T4) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed twice and incubated with an R-phycoerythrin (PE)- conjugated mouse anti-human IgG1 secondary antibody (BD Biosciences, 555787) on ice for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: Seven days after electroporation a total of 5 × 106 T cells were collected and stained in 100 uL with fluorophore-conjugated anti-human antibodies for CD3 (BD Biosciences #564001), beta-2-microglobulin (BioLegend #316306 ...
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bioRxiv - Physiology 2022Quote: Genome-edited hiPSCs were evaluated for expression of pluripotency markers using the BD Human Pluripotent Stem Cell Transcription Factor Analysis Kit (BD Biosciences, UK). In vitro differentiation efficiency was assessed by expression of stage-specific markers of definitive endoderm (CXCR4 ...
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Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Membranes were blocked in 5% BSA/0.1% Tween20 in 1x TBS or 5% milk/0.1% Tween20 in 1x PBS for primary antibody mouse anti-human phospho-eNOS (1:1,000, pS1177, #612392, BD Biosciences, Franklin Lakes, NJ) or mouse anti-human-eNOS (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro780 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated in DMEM media containing 1% FBS and 1% BSA with Alexa Fluor® 647 Mouse Anti-Human LAMP1 Clone H4A3 (BD Biosciences) for 1h at 4°C with intermittent mixing ...
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bioRxiv - Cancer Biology 2022Quote: ... Apoptotic cells in leukemic spleens or human/mouse cell lines after different treatments were quantified with PE-AnnexinV Apoptosis Detection Kit I (BD Pharmingen, 559763). All flow cytometry data was collected on an Attune NxT Flow Cytometer (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... HAECs were allowed to recover from passaging for 24 h after plating and were subsequently incubated with AlexaFluor-647 anti-human VE-Cadherin antibody (1:100, BD Biosciences, #561567). After 15 minutes incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Human whole blood was collected from adult healthy volunteers with prior consent in heparinized vacutainer blood collection tubes (BD, Franklin Lakes, NJ) in accordance with Ahir et al. ...
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bioRxiv - Cancer Biology 2019Quote: 5×105 human CD14+ cells were isolated from peripheral blood mononuclear cells (PBMC) using anti-CD14 beads (BD Biosciences, San Jose, CA) with a typical yield of ≥ 70% recovery and ≥ 90% purity ...
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bioRxiv - Biochemistry 2020Quote: ... and CD81 antibodies (with no beads) were added to the solution containing the EVs (PE Mouse Anti-Human CD81 Clone JS-81, BD Pharmingen™). After 1 h of incubation ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Immunology 2021Quote: ... and viability dye (eBioscience™ Fixable Viability Dye eFluor™ 780) followed by Foxp3 staining with anti-FoxP3 (259D/C7) using Human FoxP3 buffer set (BD biosciences) according to the Manufacturer’s recommendations.
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bioRxiv - Immunology 2021Quote: ... spleen and bone marrow were isolated and evaluated for the percentage of human CD45+ cells by flow cytometry (CD45-PerCP-Cy5.5, Biolegend Cat: 304028; BD FACS Canto II). Populations were defined and analyzed using FlowJo software (Flowjo v10.7.1 ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected after 6 days of co-culture to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... a panel of four fluorescent markers was employed including fluorescein isothiocyanate (FITC) mouse anti-human CD40 (BD Biosciences; San Jose, CA, USA), phycoerythrin (PE ...
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bioRxiv - Immunology 2022Quote: Secretion of CXCL10 (IP-10) into the medium was quantified 24 h after stimulation by ELISA (Human IP-10; BD OptEIA, 550926). Briefly ...
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bioRxiv - Immunology 2022Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA)) with the following anti-human antibodies (all at 1:200 dilution): anti-CD20-PECy7 (BD Biosciences, 335793), anti-CD3-APC-eFluro 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Immunology 2023Quote: ... were diluted 1:50 in PBS-10% FBS and IL-2 concentration was determined by ELISA assays using the OptEIA Human IL-2 ELISA commercial kit (BD Biosciences, USA) and revealed with TMB substrate (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... VE-cadherin was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD144 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Immunology 2023Quote: ... A target primer panel was constructed by combining a commercial predesigned Human T Cell Expression Primer Panel (BD Biosciences, 259 target primers) with our custom-designed primer panel (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: The purity of each NK population was checked before and after cell sorting and routinely by flow cytometry with anti-human CD16-BV421 (clone 3G8, BD Pharmagen, France), anti-human CD56-PeCy7 (clone B159 ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... Permeabilization was followed by blocking cells using 5% BSA in HBSS+ for 30 min and incubated with the primary antibody solution - Mouse anti-human CD144 (1:150; 555661, BD Biosciences, USA) for overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies used for the flow cytometry analysis of the cell surface markers were: anti-human CD11b (740965, BD OptiBuild™, 1:100), anti-human CD14 (11-0149-42 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in sorting buffer (2% FBS and 1% Penicillin-Streptomycin in DMEM) and blocked with human FcR blocking reagent (Cat#564220; BD; used 1:50) for 10 minutes at 4 °C ...