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Citations for Becton, Dickinson and Company :
2851 - 2900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were fixed with Cytofix/Cytoperm (BD Biosciences) for 30min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and isolated BD FACSAriaIII cell sorter (BD Bioscience). First ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with Fc block (2.4G2, BD) and anti-pneumococcal serotype 4 rabbit serum (Statens Serum institutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sorted with BD Influx (BD Biosciences) or FACSAria IIu (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were analyzed using an LSRII (BD Biosciences) flow cytometer recording a maximum of 10,000 Hoechst-negative cells.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were sorted using a FACSAria II (BD) and analyzed using Flowing software (v 2.5.1 ...
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bioRxiv - Molecular Biology 2024Quote: ... or a BD LSRFortessa Cell Analyzer (BD Biosciences). Flow cytometry results were analyzed using FlowJo v10 software ...
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bioRxiv - Biochemistry 2024Quote: ... and a FACSAria III cell sorter (BD Biosciences) (Murtinheira et al. ...
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bioRxiv - Immunology 2024Quote: ... we fixed cells in Cytofix/Cytoperm (BD Biosciences) and conducted intracellular staining using 1x Perm/Wash (BD ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were washed 2x with Permwash buffer (BD) and stained intracellularly for 30 min at 4C with the following markers (SI Appendix) ...
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bioRxiv - Immunology 2024Quote: ... HAP1 SPPL3-/- cells with VPD450 membrane dye (BD) and HAP1 SPPL3-/-B3GNT5-/- with CFSE according to manufacturer’s protocol while the B3GNT5-/- remained unlabelled ...
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bioRxiv - Cancer Biology 2021Quote: ... The bottom layer of soft agar was prepared by mixing 2 × growth medium and 1.5% noble agar (BD Biosciences, San Jose, CA) at a 1:1 ratio and the mixture was poured into 6-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... Passages were performed by resuspending Matrigel-embedded organoids in cold DPBS and transferring them to a Falcon tube using a 2 mL syringe with a 27 G ½ needle (BD Microlance #300635) to break them up ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Permeabilization buffer (eBioscience) and incubated for 15 min in FACS buffer (PBS, 2% FCS) before adding the primary MASH-1 antibody (BD Biosciences, 556604), 1:250 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4 μL of the saturated culture were transferred in 60 uL PBS and the samples were stored at 4 °C for 1-2 days before being assayed by flow cytometry (BD FACSCanto II). Data were analyzed in FlowJo ...
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bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for 1 hour at 4 °C with 2 µl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4x105 events were collected per sample within 3 hours after staining on an LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Strains were precultured during 48h in 300 μL of Synthetic Defined (SD) medium (Warringer et al., 2011) with some modifications (2% glucose, 0.14% Yeast Nitrogen Base (BD Difco™, U.S.A.), 0.5% ammonium sulfate ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Biophysics 2020Quote: Spectrin in neurons (Figure 2d and Supplementary Figures 2 and 5) was labelled with a mouse monoclonal primary antibody anti-β-Spectrin II (Clone 42/B-Spectrin II, BD Biosciences) for 1 h at room temperature using a 1:400 dilution in 5% BSA in PBS ...
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bioRxiv - Microbiology 2020Quote: ... 50 μL of EDTA-anticoagulated whole blood were stained with the monoclonal antibodies CD3 BV605 (SP34-2, BD Biosciences, San Jose, CA), CD4 PerCP/Cyanine5.5 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... then probed with the following primary antibodies in 5% milk/TBS-T overnight at 4 °C: SREBP-2 (1:250; BD Biosciences, 557037), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained for 1 hour at 4 °C with 2 μl of the following Abs: PE-Cy7 conjugated anti-mouse IFN-γ (BD Biosciences), PE conjugated anti-mouse IL-2 (Invitrogen eBioscience) ...
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bioRxiv - Pathology 2022Quote: ... the samples were washed and resuspended in PBS supplemented with 2% FBS for analysis on a five-laser flow cytometer (BD Biosciences, Fortessa). The data were analysed with FlowJo software (TreeStar).
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bioRxiv - Immunology 2022Quote: ... For stimulation ∼106 lymphocytes plated in medium containing 2% fetal calf serum and 1.5 μl/ml Golgiplug (BD Bioscience; San Jose, CA) were cultured with peptides each present at a final concentration of 2 µg/ml for 5 hr at 37°C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2020Quote: ... 7 and 14) or at 2 time points (days 1and 14) after vaccination into Vacutainer® Heparin tubes (BD Biosciences, Plymouth, U.K.). Blood samples were subjected to immediate preparation and analyses ...
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bioRxiv - Microbiology 2019Quote: ... for 1 h or 2 h and then subsequently incubated for 30 min on ice with an APC-conjugated rat anti-mouse IgG (BD Biosciences, CA). Stained cells were analyzed using a BD AccuriTM C6 Plus with a Sampler flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Samples were resuspended to a volume 2.5 mL with PBS with 2% FBS and 0.4% EDTA and placed on an Imag (BD Biosciences, San Diego, CA) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 5x 105 cells resuspended in 20µl PBS were locally injected into the fracture site of bone under anesthesia using a Hamilton syringe (Hamilton, USA) and a 30-gauge 1/2 needle (BD Biosciences, USA). X-rays were taken weekly using a digital X-ray machine (Faxitron X-Ray Corp. ...
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bioRxiv - Neuroscience 2020Quote: COs were washed 2 times for 5 minutes at room temperature in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Cell Biology 2019Quote: ... A second round of purification was then performed with anti-rat Dynabeads coated with rat anti-mouse ICAM-2 (rat clone 3C4; BD Biosciences #553325). Cells were cultured in Endothelial Cell Growth Medium MV2 (PromoCell ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used for the blockade of B7-1- and B7-2-dependent CD28 costimulation were anti-mouse CD80 Clone 16-10-A1 (BD Pharmingen #553736) and anti-CD86 Clone GL1 (BD Pharmingen #553689) ...
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bioRxiv - Immunology 2021Quote: ... Lymphocytes were incubated in 200 μl of a mixture of BD FACS™ Permeabilizing Solution 2 and BD FACS™ Lysing Solution prepared according to manufacturer’s instructions (BD Biosciences) for 8 min at RT ...
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bioRxiv - Immunology 2022Quote: ... then fixed with 2% paraformalin and kept on ice until acquisition on a BD Symphony A3 (Becton Dickinson BD; Franklin Lakes, NJ). Data was analyzed using FlowJo 10.8.1 (RRID:SCR_008520 ...
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bioRxiv - Cell Biology 2022Quote: ... Rings were fixed after 7 days with 2% PFA and stained with rat PECAM (dilution 1:100, Rat monoclonal, clone MEC 13.3; BD Biosciences, Cat# 557355). Rings were imaged in 3D using the ZEISS Lightsheet Z.1 microscope ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with 2% FACS buffer with 50nM dasatinib and fixed using fixation/permeabilization solution (Cytofix/Cytoperm™ fixation and permeabilization kit, BD Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... the internal space of the chambers in which biofilms were cultivated measured 5000μm x 500μm x 70μm (LxWxH). Segments of inlet tubing (Cole Parmer PTFE #30, cat. # 06417-11) attached to 27Gx1/2 needles (BD Precision, cat. # 305109) on 1mL syringes (Brandzig ...
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bioRxiv - Physiology 2022Quote: ... Samples were stored at 10-15°C in 2 mL K2-EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 15 to 45 min later.
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bioRxiv - Physiology 2022Quote: ... The sample was stored at 10-12 °C in 2 mL EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 0.5-2.5 h later ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were blocked with 2% BSA in 0.1% tween TBS (TBST) for 1h and immunostained with mouse anti-paxillin IgG1 antibody (BD Transduction Laboratories 610052) (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 menstrual endometrial fragments (diameter 1-2 mm) from donor animals were inserted using two 14G catheters (Insyte, Vialon, BD, Madrid, Spain) under laparoscopic guidance in both left and right flank and behind the uterus ...
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bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... MASP-1/-3/MAP-1 mab 8B3 or F(ab’)2 fragments generated from an irrelevant mouse IgG1 mAb clone MOPC-31C (BD Biosciences, 557273) were coated on maxisorp plates at 4 °C ON and afterwards washed and blocked for 1 hr in TBS/T ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...