Labshake search
Citations for Becton, Dickinson and Company :
2751 - 2800 of 4386 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... Cells were then incubated with conjugated antibodies for 30 minutes: SOX17-PE (561591, BD Biosciences), PDX1-PE (562161 ...
-
bioRxiv - Microbiology 2024Quote: PBMC suspensions were stained with the following antibodies: CD4-V450 (clone: RPA-T4; BD Biosciences) and CD3-PE-CF594 (clone ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The anti-p62 (610833, 1:500 for WB and IF) antibody was purchased from BD Biosciences ...
-
bioRxiv - Bioengineering 2024Quote: ... and resuspended in an antibody cocktail containing anti-RORγt (Alexa Fluor 647, BD Biosciences, 562682), anti-T-bet (Brilliant Violet 785 ...
-
bioRxiv - Cell Biology 2024Quote: Antibodies used for Western blot included mouse anti-human ERK (1:1000; 610030, BD Biosciences), rabbit anti-human phospho-Erk1/2 (Thr202/Tyr204 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and then resuspended cells in antibody diluted 1:200 in permeabilization buffer (BD Perm/Wash) with overnight incubation at 4C ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were incubated with diluted primary antibody (Anti-Vimentin, 1:100, BD Pharmingen™ #550513) overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... After Fc blocking with anti-CD16/32 antibody for 15 min (clone: 2.4G2; BD Pharmingen), the platelets were washed and stained with anti-CD41-FITC/anti-CD42b-PE ...
-
bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were used: Alexa Fluor 647-Sox2 (BD Biosciences, #562139, 1:100), BrdU (Abcam ab6326 ...
-
bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
-
bioRxiv - Immunology 2020Quote: ... spleen cells were ex vivo stimulated for 20 h with 40 μg/ml PPD (purified protein derivative, Statens Serum Institute, Denmark) in the presence of Golgi Stop™ (BD Biosciences) and Monensin (1000x ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking solution of 1% normal horse serum in PBS with 0.3% Triton X-100 and incubated overnight at 37°C as follows: mouse (IgG1) anti-CtBP2 (C-terminal Binding Protein) 1:200 (#612044, BD Transduction Labs) targeting pre-synaptic ribbons ...
-
bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
-
bioRxiv - Immunology 2021Quote: ... Intracellular proteins were stained for 60 min at room temperature after permeabilization and fixation with BD Cytofix/Cytoperm Plus (BD Biosciences, 555028) using the following antibodies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells expressing the relevant surface receptor or fluorescence protein were sorted using a BD FACSMelody or a BD FACSAria SORP Cell Sorter (BD Biosciences, USA). Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
-
bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
-
bioRxiv - Cancer Biology 2022Quote: ... Stable cells expressing fluorescent reporter proteins (mCherry or eGFP) were sorted using BD FACSAria Flow Cytometer (BD Bioscience, Mountain View, CA, USA).
-
bioRxiv - Cell Biology 2023Quote: ... Protein blots were visualized using Western ECL Substrate Kit (Thermo Pierce) and exposed using a ChemiDoc XRS+ system (BD, Franklin Lakes, NJ). The intensity of bands was quantified by Image J software
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
-
bioRxiv - Immunology 2020Quote: ... Supernatant was collected and secreted TNFα protein levels were measured using a BD OptEIA Mouse TNFα Enzyme-Linked Immunosorbent Assay (ELISA) kit (BD Biosciences, Mississauga, ON). Triplicates wells were used for each stimulation condition ...
-
bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
-
bioRxiv - Immunology 2023Quote: ... Analysis of intracellular antigens was done by using the same fixation and permeabilization as was described under “Metabolic labeling of nascent proteins” or using BD Cytofix/Cytoperm fixation/permeabilization kit (BD Biosciences, cat#554714). Maximum of 3 x 106 cells per reaction ...
-
bioRxiv - Immunology 2024Quote: Cells were seeded in a 96 well plate with CM media containing the protein transport inhibitor monensin (BD GolgiStop™, 1 in 1000) and CD107a antibody (0.75 μg/ml) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Following secondary antibodies (all 1:500) were used: Biotinylated Goat Anti-Rat Ig (559286, BD Pharmingen) and Biotinylated Goat Anti-Mouse Ig (E0433 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Newly replicated DNA was stained for 2 hours with primary antibodies anti-IdU (mouse, BD Biosciences) and anti-CldU (rat ...
-
bioRxiv - Cancer Biology 2021Quote: ... Antibody staining was performed on ice for 30’ with CD44-APC (clone IM7, 559250, BD Pharmingen) and EpCAM-FITC (ESA-214 ...
-
bioRxiv - Cell Biology 2020Quote: ... CD11b-APC (clone M1/70) and CD11c-APC-H7 (clone HL3) antibodies (all from BD Biosciences). Cells were analysed on a BD FACSVerse flow cytometer and FlowJo software.
-
bioRxiv - Immunology 2021Quote: ... CD86 surface expression level was assessed with an anti-CD86 antibody (clone 2331, APC, BD Biosciences). For dextran uptake assays ...
-
bioRxiv - Immunology 2021Quote: Fluorescence antibody-stained single-cell suspensions were analyzed using LSRFortessa or LSRII flow cytometers (BD Biosciences). For live cell analysis ...
-
bioRxiv - Genetics 2021Quote: ... they were incubated overnight at room temperature with primary antibodies: mouse anti-human MLH1 (BD Biosciences) diluted 1:100 and rabbit anti-SCP3 (Abcam ...
-
bioRxiv - Developmental Biology 2020Quote: ... Then they were incubated with primary antibodies (anti-ICAM-II (BD Pharmingen™, 553326, 1:100), anti- Collagen-Type IV (Collagen-IV ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies and dilutions (in PBT/BSA) were: E-Cadherin (CDH1) 1:200 (BD Biosciences, 610181), anti-BAP1 1:200 (Cell Signaling ...
-
bioRxiv - Developmental Biology 2021Quote: ... Sections were then incubated overnight at 4°’C with primary antibodies targeting BrdU (BD Transduction Laboratories), GFP (Aves Labs ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Antibodies are as follows: BV421 Mouse anti-human NFκB pS529 clone K10-895.12.50 (BD Biosciences #565446), PerCP-Cy5.5 Mouse anti-human ERK1/2 pT202/pY204 clone 20A (BD Biosciences #560115) ...
-
bioRxiv - Molecular Biology 2021Quote: The muscle sections were stained with the primary anti-CD31 antibody (1:500, 550274, BD Biosciences) and counterstained with DAPI as described previously[24] ...
-
bioRxiv - Cell Biology 2022Quote: ... The following primary and secondary antibodies were used: anti-SMN (1:1000, BD Transduction Laboratories #610647), anti-ND1 (1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... The following primary and secondary antibodies were used: anti-SMN (1:1000, BD Transduction Laboratories #610647), anti-Fast Myosin Skeletal Heavy chain (1:1000 ...
-
bioRxiv - Immunology 2021Quote: Fluorescently labelled antibodies against the following mouse antigens were used: CD3 (clone 145-2C11, BD Biosciences), CD4 (clone RM4-5 ...
-
bioRxiv - Immunology 2021Quote: ... B cells were also stained with following anti-human antibodies: anti-IgG-PECF594 (BD biosciences, 562538), anti-IgM-AF700 (Biolegend ...
-
bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used to immunostain mouse tissue: CD31 (1:100, rat, BD Biosciences), GFAP (1:200 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies used for immunocytochemistry are as follows: mouse anti-PAX6 1:200 (561462, BD Biosciences), rabbit anti-FABP7 (BLBP ...
-
bioRxiv - Cancer Biology 2020Quote: ... and cells were stained with the indicated antibodies and analyzed by LSRFortessa X-20 (BD Biosciences) or BD Verse (BD Biosciences) ...
-
bioRxiv - Neuroscience 2021Quote: ... Incubation with primary antibody was performed overnight at 4°C [aSyn (1:1000, 610787, BD Biosciences), aSyn (phospho S129 ...
-
bioRxiv - Neuroscience 2021Quote: ... while endogenous TNIK was pulled-down with 10 µL of anti-TNIK antibody (BD Biosciences, #612250) and 25 µL Dynabeads Protein G (Invitrogen ...
-
bioRxiv - Cancer Biology 2020Quote: ... washed with RPMI and incubated with an anti-CD16/CD32 antibody (1:200, BD Biosciences #564220) in FACS buffer (PBS containing salts ...
-
bioRxiv - Cancer Biology 2020Quote: ... cell suspensions were incubated for 30 min with an antibody cocktail in Stain Buffer (BD Pharmingen). For cell surface antigens the following anti-mouse antibodies were used ...
-
bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained with fluorescently labeled antibodies against BCL-2 (clone Bcl-2/100, BD Bioscience), BCL-XL (clone 54H11 ...
-
bioRxiv - Microbiology 2021Quote: ... paxillin was stained with a mouse anti-paxillin antibody (1:250, BD Biosciences, San Jose, CA), and phospho-paxillin was stained with a rabbit anti-phospho-paxillin antibody (Y118 ...