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Citations for Becton, Dickinson and Company :
2701 - 2750 of 3574 citations for Rat Integrin Alpha 2 ITGa2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... 2% dextrose) when containing no plasmids and in synthetic defined (SD) media (0.67% yeast nitrogen base without amino acids [BD Difco], 2% dextrose) supplemented with the appropriate yeast synthetic drop-out medium supplement when plasmid maintenance was required (SDΔW-without tryptophan ...
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bioRxiv - Cancer Biology 2024Quote: ... Positively transduced cells were selected with 2 µg/ml puromycin followed by a sterile sort for high GFP and mCherry expression (BD FACSAria II). Expression was maintained with 1 µg/ml puromycin in all experiments except where immune cells were needed.
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD, Cat#566385) for 30 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with PBS (2%FBS) and resuspended in 100ul ice-cold BD Phosflow™ Perm Buffer III (BD biosciences, USA) and incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in sorting buffer (2% FBS and 1% Penicillin-Streptomycin in DMEM) and blocked with human FcR blocking reagent (Cat#564220; BD; used 1:50) for 10 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... cell suspensions were resuspended in PBS containing 2% FBS and then separated on a FACSAria Fusion flow cytometer (BD Biosciences, San Jose, CA). For single cell sorting ...
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bioRxiv - Cancer Biology 2019Quote: ... of both preparations was determined by titration onto the target cell line and quantification of the percentage of BFP positive cells 2-3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Cell Biology 2019Quote: ... for 2 hours at room temperature followed by overnight incubation at 4°C with anti-PECAM-1 (MEC13.3, BD Bioscience #550274, 1:250) diluted in blocking buffer ...
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bioRxiv - Genomics 2021Quote: ... anti-CD3 (SP34-2: Alexa700; BD Biosciences, Custom Bulk 624040, PerCP-Cy5.5; BD Biosciences, Custom Bulk 624060, and Pacific Blue; BD Biosciences, Custom Bulk 624034), anti-CD4 (L200 ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed and resuspended in PBS supplemented with 2% FCS for FACS analysis (FACSCanto II; BD Bioscience, San Jose, USA). FACS data analysis was performed using BD FACSDivaTM Software 8 (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4×105 events were collected per sample within 3 hours after staining on a LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Microbiology 2019Quote: Spiral plating was conducted using an Autoplate 400 Eddy Jet 2 spiral plater (IUL, Barcelona, Spain) onto Lactobacilli deMan Rogosa and Sharpe (MRS) agar (Becton, Dickinson and Company) supplemented with 1% cycloheximide (Remel ...
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bioRxiv - Systems Biology 2022Quote: ... coli strain JM109 (1 × 1010 colony-forming units (CFU)/ml) were cultured for various times in 2 ml of 2xYT liquid medium (BD Difco, Cat# 244020) containing various concentrations of pyridoxal 5’ -phosphate (pH adjusted to 7.0) ...
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bioRxiv - Microbiology 2020Quote: ... Cultures with vesicles were incubated for 2 hours at the normal growth temperatures and examined on an Influx flow cytometer (Cytopeia/BD, Franklin Lakes, NJ). Cells were excited using a blue 488 nm laser and monitored for chlorophyll (692/40 nm emission ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation for 2 hours at room temperature with primary antibody (SOX2 [1:200, EMD Millipore AB5603], NESTIN [1:200, BD Pharmingen #556309]). After washing five times with PBS/0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: The HLA-A2 status was confirmed by PBMCs staining with 2 μL of anti-HLA-A2 mAb (clone BB7.2) (BD Pharmingen, Franklin Lakes, NJ), at 4°C for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Between 2 - 4×105 events were collected per sample within 24 hours after staining on a LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were immediately split into 3 fractions: 2 ml were transferred to heparin and EDTA tubes respectively (both BD, Franklin Lakes, New Jersey). Heparin samples were then centrifuged (1000x g ...
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bioRxiv - Cancer Biology 2019Quote: ... A pure polyclonal population of dCas9-KRAB expressing cells was generated by 2 rounds of fluorescence activated cell sorting (FACS) gated on the top half of BFP positive cells (BD FACS Aria II). The performance of A375_i in knocking down endogenous genes was evaluated by individually targeting 3 control genes (ST3GAL4 ...
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bioRxiv - Cell Biology 2019Quote: The following antibodies were used for fluorescence microscopy: mouse IgG2B anti-human DC-SIGN at 2 μg/ml (DCN46, BD Biosciences, Breda, Netherlands), mouse IgG1 anti-human AZN-D1 at 2 μg/ml (Geijtenbeek et al. ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... approximately 200 μL cells were added into the flow tube containing 2 mL PBS and tested by the flow cytometry (BD, New Jersey, USA). Three independent assays were performed.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with Hank’s buffered saline solution and resuspended in 2mM EDTA/ 2% BSA/ PBS buffer containing [2µg/mL] propidium iodide (PI) (BD Biosciences, Cat. No. 556364) and [1µM] Calcein violet (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Female C57BL/6J mice aged 8 – 12 weeks were injected intraperitoneally with 2 ml each of 3% sterile thioglycollate (BD Biosciences, catalog # 211716) medium ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were diluted in basal medium containing 2% dextran-coated charcoal-stripped serum (DCC) (HyClone Laboratories, Logan, UT) and plated into culture dishes (BD Falcon, Bedford, MA). Cells were cultured for 24 hours in an incubator at 37°C with saturating humidity and 5% CO2.
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bioRxiv - Immunology 2022Quote: ... Spleens were disrupted using a syringe in 2% FBS/PBS and passed through a 40 μm nylon cell strainer (BD, catalog no. 352340) to obtain a single-cell suspension ...
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bioRxiv - Microbiology 2023Quote: ... which were grown in solid Lysogeny broth (LB) medium (5.0 g L−1 yeast extract, 10 g L−1 Bacto tryptone, 2 % Bacto agar (BD Biosciences, Franklin Lakes, NJ), 5.0 g L−1 NaCl ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences, 557735], and anti-CD107a [BD Biosciences, 560647]) by 20 minutes of incubation at room temperature ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Pathology 2023Quote: ... Cells were diluted in basal medium containing 2% dextran-coated charcoal-stripped serum (DCC) (HyClone Laboratories, Logan, UT) and plated into culture dishes (BD Falcon, Bedford, MA). Cells were cultured for 24 hours in an incubator at 37°C with saturating humidity and 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... digested cells were resuspended in FACS buffer (2% FBS–PBS) and analyzed by a FACS Calibur flow cytometer (Becton, Dickinson and Company, USA). Compensation and analysis of results were performed using Flow Jo software.
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed for 10 min in 2% paraformaldehyde and were analyzed using a FACS CANTO II (BD Biosciences, Franklin Lakes, NJ, USA). For lentiviral transduction efficiency assessment ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... of both preparations was determined by titration onto target cell lines and quantification of the percentage of BFP+ cells 2–3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Immunology 2023Quote: ... Naïve T cells were resuspended in complete medium (KOHJIN BIO DMEM-H (16003016) + 10% FBS) and activated in the presence of 2 μg/mL of anti-CD3 (BD Pharmingen, REF: 553058) and 2 μg/mL of anti-CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... the internal space of the chambers in which biofilms were incubated measured 5,000 μm × 500 μm × 70 μm (LxWxH). Segments of inlet tubing (Cole Parmer PTFE #30, cat. # 06417–11) attached to 27Gx1/2 needles (BD Precision, cat. # 305109) on 1 milliliter syringes (Brandzig ...
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bioRxiv - Neuroscience 2024Quote: ... 250,000-350,000 donor cells or the same volume of empty HBSS+2% FBS vehicle were intraperitoneally (IP) injected using a 30 G insulin syringe (BD Biosciences cat# 328431). Transplanted cells were allowed 2 weeks to engraft prior to SCI.
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bioRxiv - Cell Biology 2020Quote: ... cells were processed using the Cell Cycle and BrdU Flow Kit (BD Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Eosinophils were labelled with sample tags (633793 BD Mouse Single-Cell Multiplexing Kit) according to the the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Then cells were fixed and permeabilized with the Fixation/Permeation kit (BD Biosciences) and stained with IFNγ (PerCP/Cy5.5 ...
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bioRxiv - Immunology 2021Quote: ... Cells were further stained with the APC BrdU Flow Kit (552598, BD Biosciences) following the manufacturer’s guidelines for analysis ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining was performed using the BD Cytofix/Cytoperm Solution Kit (BD Biosciences) and the following antibodies ...
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bioRxiv - Immunology 2020Quote: ... fixed and permeabilized with the Cytofix/Cytoperm™ Fixation/ Permeabilization Solution Kit (BD) for 15min at 4°C and stained subsequently for another 30min with anti-MPO eFluor450 (455-BE6) ...
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bioRxiv - Immunology 2020Quote: ... Incorporation of BrdU was detected using the FITC BrdU Flow Kit (BD Pharmingen) and analyzed by flow cytometry.
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bioRxiv - Immunology 2020Quote: ... intracellular HIV-p24 staining was performed using the Cytofix/CytopermTM kit (BD #554714). Flow cytometry was performed using BD LSRFortessa or BD accuri c6 plus ...