6 methyl N1 4 pyridin 3 yl thiazol 2 yl benzene 1 3 diamine citations for Becton, Dickinson and Company

Quote: ... at 5:1:1 concentration ratio before transferred into a 1 ml syringe with BD Luer-Lok (BD, 309628, NJ, USA). The syringe was then hanged vertically to allow cell settling by gravity for 10-15 min with no flow ...

Time-dependent roles of adolescent- and adult-born dentate granule neurons in spatial learning

Nuria Masachs, et al., bioRxiv - Neuroscience 2020

Quote: ... one section out of ten was incubated with BrdU antibodies (BrdU, 1/1,000, CldU, 1/500, Accurate Chemical; IdU, 1/500, BD Bioscience). Bound antibodies were visualized with Cy3-goat anti-rat antibodies (1/1,000 ...

Environmental and taxonomic drivers of bacterial extracellular vesicle production in marine ecosystems

Steven J. Biller, et al., bioRxiv - Microbiology 2022

Quote: ... were grown in in Pro99 media supplemented with 5 g L-1 peptone and 1 g L-1 yeast extract (BD Difco). All heterotrophs were grown at 24 °C ...

Ex vivo detection of SARS-CoV-2-specific CD8+ T cells: rapid induction, prolonged contraction, and formation of functional memory

Isabel Schulien, et al., bioRxiv - Immunology 2020

Quote: ... A fixable Viability Dye (APC-eFluor780 1:200, 1:400) (eBioscience, Germany) or ViaProbe (7-AAD, 1:33) (BD Biosciences, Germany)) was used for live/dead discrimination ...

Helicobacter hepaticus as disease driver in a novel CD40-mediated model of colitis

Verena Friedrich, et al., bioRxiv - Immunology 2020

Quote: ... RORγt (AFKJS-9; PE, dil. 1:100) (eBioscience); CD25 (PC61; PerCP, dil. 1:400), CD103 (M290; PE, dil. 1:150) (BD Pharmingen); CD11b (M1/70 ...

Time-resolved single-cell transcriptomics uncovers dynamics of cardiac neutrophil diversity in murine myocardial infarction

Ehsan Vafadarnejad, et al., bioRxiv - Immunology 2019

Quote: ... after which cells were stained with Fixable Viability Staining e780 (1:1000) and CD11B (M1/70) antibodies: CD11B-BV510 (Day 1 cells, 1:300, BD Biosciences), CD11B-Percp-Cy5.5 (Day 3 cells ...

LDL-Binding IL-10 Reduces Vascular Inflammation in Atherosclerotic Mice

Lisa R. Volpatti, et al., bioRxiv - Bioengineering 2024

Quote: ... and stained for 30 min on ice with surface antibodies at a 1:200 dilution in a 1:1 mixture of FACS buffer and Brilliant Stain Buffer (BD Biosciences). Cells were washed with FACS buffer and PBS and fixed with 2% paraformaldehyde on ice for 20 min ...

A Structural Classification of the Variant Surface Glycoproteins of the African Trypanosome

Sara Đaković, et al., bioRxiv - Microbiology 2023

Quote: ... 1 x 106 parasites were collected and incubated with VSG2WT antisera (1:4000) or VSG11WT (1:1000) together with Fc block (1:200, BD Pharmingen) in cold HMI-9 without FBS for 10 min at 4°C ...

Embedding biomimetic vascular networks via coaxial sacrificial writing into functional tissue

Paul P. Stankey, et al., bioRxiv - Bioengineering 2024

Quote: ... The cOBBs were resuspended in 1:1 v/v ECM gel to cOBBs and transferred to a 1 mL disposable syringe (BD Biosciences). The cOBBs were centrifuged at 100g for 3 min and the supernatant removed ...

Development of an orally bioavailable mSWI/SNF ATPase degrader and acquired mechanisms of resistance in prostate cancer

Tongchen He, et al., bioRxiv - Cancer Biology 2024

Quote: ... bilateral subcutaneous dorsal flank tumors were established by injecting 1 × 106 cells suspended in a 1:1 mixture of serum-free medium and Matrigel (BD Biosciences). Once tumors reached an average volume of 100 mm3 ...

Engineered Flt3L Drives Tolerogenic State to Attenuate Anti-drug Antibody Responses

Aaron T. Alpar, et al., bioRxiv - Bioengineering 2024

Quote: ... Viability dye was quenched by washing with FACS buffer (PBS + 2% FBS + 1 mM EDTA) before addition of surface staining antibodies in a 1:1 dilution of Brilliant Stain Buffer (BD 563794) in FACS buffer ...

Extracellular vesicles mediate OxLDL-induced stromal cell proliferation in Benign Prostatic Hyperplasia

Franco F. Roldán Gallardo, et al., bioRxiv - Cell Biology 2024

Quote: ... and subsequentenly with primary antibodies (1/30 Ki-67 Cat. 550609 and 1/200 Anti BrdU Cat. 555627, both in 1% BSA, BD Pharmingen) overnight ...

A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins

Etsuko Shibata, Anindya Dutta, bioRxiv - Molecular Biology 2020

Quote: ... DNA was denatured in 2 M hydrochloric acid and stained with FITC-conjugated BrdU antibody (556028, BD Biosciences) and propidium iodide (MilliporeSigma ...

Altered frequency of CD24^highCD38^high transitional B cells in patients with cardiac involvement of chronic Chagas disease

Magalí C. Girard, et al., bioRxiv - Immunology 2019

Quote: ... A minimum of 2×105 events within the lymphocyte population were acquired in a FACSCanto II (BD Biosciences) flow cytometer using FACS Diva Software (BD Biosciences) ...

The human UDP-galactose 4’-epimerase (GALE) is required for cell surface glycome structure and function

Alex Broussard, et al., bioRxiv - Cell Biology 2019

Quote: ... cells were fixed with 2% paraformaldehyde for 20 minutes before analysis on a FACSCanto II cytometer (BD biosciences). The antibodies used were Fas (Biolegend 305602 ...

Inflammation induces pro-NETotic neutrophils via TNFR2 signaling

Friederike Neuenfeldt, et al., bioRxiv - Immunology 2021

Quote: ... Red blood cells were removed by adding 2 ml of BD FACS Lysing Solution (BD Bioscience, Heidelberg, Germany) for 10 min at room temperature ...

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