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Citations for Becton, Dickinson and Company :
2601 - 2650 of 4284 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A non-invasive method to sample immune cells in the lower female genital tract using menstrual discsbioRxiv - Immunology 2024Quote: ... Single-stained compensation controls were prepared for each experiment using antibody capture beads (BD Biosciences) diluted in FACS buffer and amine reactive beads (ArC™ Amine Reactive Compensation Bead Kit ...
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bioRxiv - Immunology 2024Quote: ... followed by the addition of a mastermix of fluorescently tagged antibodies (Table S2, BD Aria). Stained cells were analyzed using a four-laser BD FACS ARIA III with a cell sorter ...
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bioRxiv - Molecular Biology 2024Quote: ... B cells were isolated from Spleen using biotinylated anti-CD19 antibody (BD Pharmingen, clone ID3) or the BD IMagTM Biotinylated Mouse B Lymphocyte Enrichment Cocktail and BD IMAGTM Streptavidin Particles Plus ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated overnight with primary antibodies against EPS8 (BD Biosciences, 610143, at 1:100), GNAI3 (Millipore Sigma ...
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bioRxiv - Synthetic Biology 2024Quote: ... the following monoclonal antibodies (mAbs) were utilized: BV711 mouse anti-human CD3 (BD Bioscience, 563725), BV605 mouse anti-human CD4 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with surface antibodies (Table S3) diluted in Brilliant Buffer (BD Biosciences, 566349) for 30 minutes at 4 °C protected from light ...
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bioRxiv - Genetics 2024Quote: ... The following antibody mixtures were used for B cell (FITC rat anti-mouse CD43, BD Pharmingen 553270 ...
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bioRxiv - Immunology 2024Quote: ... BrdU was determined after surface staining followed by antibody detection according to manufacturer’s directions (BD). Analysis of acquisition events was accomplished using FlowJo software (Tree Star ...
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bioRxiv - Immunology 2024Quote: ... followed by staining with a biotinylated anti-rat IgG2A antibody (clone RG7/1.30, BD Biosciences), and finally streptavidin-PE-Cy7 (BioLegend).For LINE-1 ORF1p and MLV envelope ...
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bioRxiv - Neuroscience 2024Quote: ... Single cell suspensions were then blocked with rat anti-mouse CD16/CD32 antibodies (BD Biosciences) used at the recommended dilution for 12 min before incubation with primary antibodies diluted at 1/200 in FACS buffer (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and non-specific antibody binding was blocked with anti-mouse CD16/CD32 (BD Pharmingen #553142) at 1:100 dilution for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2024Quote: The following primary antibodies were used: anti-CD45 BV421 (1:200, Cat#563890, BD Biosciences), anti-CD11b PerCP Cy5.5 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... Samples were stained for 30min on ice using the following antibodies: anti-CD3-PE (BD), anti-CD4 BV510 (BD) ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies used were anti-mouse or rabbit Alexa Fluor 488 and 568 (BD Biosciences). Nuclei were visualized by 4.6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Synthetic Biology 2024Quote: ... The anti-p62 (610833, 1:500 for WB and IF) antibody was purchased from BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Immunoblotting was performed using the following primary antibodies: anti-VHR (DUSP3) (1:1000, BD Biosciences), anti-NPM (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Antibody-stained cells were analyzed on either FACS Canto II or Melody instruments (BD Biosciences). Data were analyzed using FlowJo 10 analysis software (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... and subsequently stained with fluorophore-conjugated or biotinylated antibodies (BioLegend, Thermo Fisher Scientific, BD Biosciences). Where indicated ...
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bioRxiv - Immunology 2024Quote: ... After 24 hours cells were incubated with Fc receptor-blocking antiCD16/CD32 antibody (BD Pharmingen) in FACS buffer for 15 minutes at 4⁰C before staining with fluorochrome-conjugated antibodies (Table 2 ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used for immunofluorescence were mouse anti-E-cadherin (1:100, BD Biosciences Clone 36), rabbit anti-Olfm4 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... The mixed receptor panel included the following antibodies: CD38(HB7)-BUV395 (BD Bioscience (San Jose)) ...
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bioRxiv - Immunology 2024Quote: ... While the activating receptor panel contained following antibodies: CD3(UCHT1)-BUV563 (BD Bioscience (San Jose)) ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with primary antibodies: α-Ccnd1 (monoclonal DCS-6, BD biosciences, 556470; 1:500), α-Ccnd1 (polyclonal antibody ABE52 ...
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bioRxiv - Microbiology 2024Quote: PBMC suspensions were stained with the following antibodies: CD4-V450 (clone: RPA-T4; BD Biosciences) and CD3-PE-CF594 (clone ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then incubated with conjugated antibodies for 30 minutes: SOX17-PE (561591, BD Biosciences), PDX1-PE (562161 ...
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bioRxiv - Cell Biology 2024Quote: Antibodies used for Western blot included mouse anti-human ERK (1:1000; 610030, BD Biosciences), rabbit anti-human phospho-Erk1/2 (Thr202/Tyr204 ...
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bioRxiv - Bioengineering 2024Quote: ... and resuspended in an antibody cocktail containing anti-RORγt (Alexa Fluor 647, BD Biosciences, 562682), anti-T-bet (Brilliant Violet 785 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then resuspended cells in antibody diluted 1:200 in permeabilization buffer (BD Perm/Wash) with overnight incubation at 4C ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were used: Alexa Fluor 647-Sox2 (BD Biosciences, #562139, 1:100), BrdU (Abcam ab6326 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with diluted primary antibody (Anti-Vimentin, 1:100, BD Pharmingen™ #550513) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... After Fc blocking with anti-CD16/32 antibody for 15 min (clone: 2.4G2; BD Pharmingen), the platelets were washed and stained with anti-CD41-FITC/anti-CD42b-PE ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were ex vivo stimulated for 20 h with 40 μg/ml PPD (purified protein derivative, Statens Serum Institute, Denmark) in the presence of Golgi Stop™ (BD Biosciences) and Monensin (1000x ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1µM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilisation step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in blocking solution of 1% normal horse serum in PBS with 0.3% Triton X-100 and incubated overnight at 37°C as follows: mouse (IgG1) anti-CtBP2 (C-terminal Binding Protein) 1:200 (#612044, BD Transduction Labs) targeting pre-synaptic ribbons ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2021Quote: ... Intracellular proteins were stained for 60 min at room temperature after permeabilization and fixation with BD Cytofix/Cytoperm Plus (BD Biosciences, 555028) using the following antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Stable cells expressing fluorescent reporter proteins (mCherry or eGFP) were sorted using BD FACSAria Flow Cytometer (BD Bioscience, Mountain View, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells expressing the relevant surface receptor or fluorescence protein were sorted using a BD FACSMelody or a BD FACSAria SORP Cell Sorter (BD Biosciences, USA). Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G ...
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bioRxiv - Cell Biology 2023Quote: ... Protein blots were visualized using Western ECL Substrate Kit (Thermo Pierce) and exposed using a ChemiDoc XRS+ system (BD, Franklin Lakes, NJ). The intensity of bands was quantified by Image J software
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Supernatant was collected and secreted TNFα protein levels were measured using a BD OptEIA Mouse TNFα Enzyme-Linked Immunosorbent Assay (ELISA) kit (BD Biosciences, Mississauga, ON). Triplicates wells were used for each stimulation condition ...
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bioRxiv - Immunology 2023Quote: ... Analysis of intracellular antigens was done by using the same fixation and permeabilization as was described under “Metabolic labeling of nascent proteins” or using BD Cytofix/Cytoperm fixation/permeabilization kit (BD Biosciences, cat#554714). Maximum of 3 x 106 cells per reaction ...
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bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
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bioRxiv - Immunology 2024Quote: Cells were seeded in a 96 well plate with CM media containing the protein transport inhibitor monensin (BD GolgiStop™, 1 in 1000) and CD107a antibody (0.75 μg/ml) ...
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bioRxiv - Cancer Biology 2021Quote: ... Following secondary antibodies (all 1:500) were used: Biotinylated Goat Anti-Rat Ig (559286, BD Pharmingen) and Biotinylated Goat Anti-Mouse Ig (E0433 ...
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bioRxiv - Cancer Biology 2021Quote: ... Newly replicated DNA was stained for 2 hours with primary antibodies anti-IdU (mouse, BD Biosciences) and anti-CldU (rat ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody staining was performed on ice for 30’ with CD44-APC (clone IM7, 559250, BD Pharmingen) and EpCAM-FITC (ESA-214 ...