6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate citations for Becton, Dickinson and Company

Quote: ... NP-FB23-2 (3 mg/kg) was delivered through the retro-orbital sinus into neonatal mice with a 31-gauge insulin syringe (BD Biosciences, San Jose, CA, USA).

An in-vitro BBB-on-a-chip open model of human blood-brain barrier enabling advanced optical imaging

Mootaz Salman, et al., bioRxiv - Bioengineering 2020

Quote: ... at 5:1:1 concentration ratio before transferred into a 1 ml syringe with BD Luer-Lok (BD, 309628, NJ, USA). The syringe was then hanged vertically to allow cell settling by gravity for 10-15 min with no flow ...

Time-dependent roles of adolescent- and adult-born dentate granule neurons in spatial learning

Nuria Masachs, et al., bioRxiv - Neuroscience 2020

Quote: ... one section out of ten was incubated with BrdU antibodies (BrdU, 1/1,000, CldU, 1/500, Accurate Chemical; IdU, 1/500, BD Bioscience). Bound antibodies were visualized with Cy3-goat anti-rat antibodies (1/1,000 ...

Environmental and taxonomic drivers of bacterial extracellular vesicle production in marine ecosystems

Steven J. Biller, et al., bioRxiv - Microbiology 2022

Quote: ... were grown in in Pro99 media supplemented with 5 g L-1 peptone and 1 g L-1 yeast extract (BD Difco). All heterotrophs were grown at 24 °C ...

Loss of LGR5 through plasticity or gene ablation is associated with therapy resistance and enhanced MET-STAT3 signaling in colorectal cancer cells

Tressie A. Posey, et al., bioRxiv - Cancer Biology 2022

Quote: ... were subcutaneously inoculated with 1 × 106 LoVo or 2 × 106 LS180 cells in 1:1 mixture of PBS and matrigel (BD Biosciences) into lower right flank ...

Ex vivo detection of SARS-CoV-2-specific CD8+ T cells: rapid induction, prolonged contraction, and formation of functional memory

Isabel Schulien, et al., bioRxiv - Immunology 2020

Quote: ... A fixable Viability Dye (APC-eFluor780 1:200, 1:400) (eBioscience, Germany) or ViaProbe (7-AAD, 1:33) (BD Biosciences, Germany)) was used for live/dead discrimination ...

Time-resolved single-cell transcriptomics uncovers dynamics of cardiac neutrophil diversity in murine myocardial infarction

Ehsan Vafadarnejad, et al., bioRxiv - Immunology 2019

Quote: ... after which cells were stained with Fixable Viability Staining e780 (1:1000) and CD11B (M1/70) antibodies: CD11B-BV510 (Day 1 cells, 1:300, BD Biosciences), CD11B-Percp-Cy5.5 (Day 3 cells ...

Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV

Aljawharah Alrubayyi, et al., bioRxiv - Immunology 2021

Quote: ... as a negative control in the presence of αCD28/αCD49d co-Stim antibodies (1 μg ml−1) GolgiStop (containing Monensin, 2 μmol/L), GolgiPlug (containing brefeldin A, 10 μg ml−1) (BD Biosciences) and anti-CD107α BV421 antibody (BD Biosciences) ...

Engineered Flt3L Drives Tolerogenic State to Attenuate Anti-drug Antibody Responses

Aaron T. Alpar, et al., bioRxiv - Bioengineering 2024

Quote: ... Viability dye was quenched by washing with FACS buffer (PBS + 2% FBS + 1 mM EDTA) before addition of surface staining antibodies in a 1:1 dilution of Brilliant Stain Buffer (BD 563794) in FACS buffer ...

LDL-Binding IL-10 Reduces Vascular Inflammation in Atherosclerotic Mice

Lisa R. Volpatti, et al., bioRxiv - Bioengineering 2024

Quote: ... and stained for 30 min on ice with surface antibodies at a 1:200 dilution in a 1:1 mixture of FACS buffer and Brilliant Stain Buffer (BD Biosciences). Cells were washed with FACS buffer and PBS and fixed with 2% paraformaldehyde on ice for 20 min ...

Embedding biomimetic vascular networks via coaxial sacrificial writing into functional tissue

Paul P. Stankey, et al., bioRxiv - Bioengineering 2024

Quote: ... The cOBBs were resuspended in 1:1 v/v ECM gel to cOBBs and transferred to a 1 mL disposable syringe (BD Biosciences). The cOBBs were centrifuged at 100g for 3 min and the supernatant removed ...

Development of an orally bioavailable mSWI/SNF ATPase degrader and acquired mechanisms of resistance in prostate cancer

Tongchen He, et al., bioRxiv - Cancer Biology 2024

Quote: ... bilateral subcutaneous dorsal flank tumors were established by injecting 1 × 106 cells suspended in a 1:1 mixture of serum-free medium and Matrigel (BD Biosciences). Once tumors reached an average volume of 100 mm3 ...

A Structural Classification of the Variant Surface Glycoproteins of the African Trypanosome

Sara Đaković, et al., bioRxiv - Microbiology 2023

Quote: ... 1 x 106 parasites were collected and incubated with VSG2WT antisera (1:4000) or VSG11WT (1:1000) together with Fc block (1:200, BD Pharmingen) in cold HMI-9 without FBS for 10 min at 4°C ...

Extracellular vesicles mediate OxLDL-induced stromal cell proliferation in Benign Prostatic Hyperplasia

Franco F. Roldán Gallardo, et al., bioRxiv - Cell Biology 2024

Quote: ... and subsequentenly with primary antibodies (1/30 Ki-67 Cat. 550609 and 1/200 Anti BrdU Cat. 555627, both in 1% BSA, BD Pharmingen) overnight ...

Infection with CagA⁺ Helicobacter pylori induces epithelial to mesenchymal transition in human cholangiocytes

Prissadee Thanaphongdecha, et al., bioRxiv - Molecular Biology 2020

Quote: ... The base of the UC of the CIM plate was coated with 20% solution of a basement membrane matrix (BD Matrigel, BD Biosciences, San Jose, CA) in serum-free medium ...

A large panel of chicken cells are invaded in vivo by Salmonella Typhimurium even when depleted of all known invasion factors

S. M. Roche, et al., bioRxiv - Microbiology 2021

Quote: ... All samples were then filtered through 60-µm nylon Blutex just before flow cytometric analyses were performed using a BD LSR Fortessa™X-20 (BD Biosciences, San Jose, CA, USA). BD FACSDiva™software (v 8.0.2 ...

Evaluation of nucleosome concentrations in healthy dogs and dogs with cancer

H Wilson-Robles, et al., bioRxiv - Cancer Biology 2020

Quote: ... the first blood collection included 20 mL of blood from 6 dogs separated into EDTA plasma (lavender top) or serum tubes (red top) (Becton, Dickinson and Company, Franklin Lakes, NJ). Nine time points were evaluated from each sample type ...

Labshake search