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Citations for Becton, Dickinson and Company :
2501 - 2550 of 2556 citations for 2 Chloro 3 4 cyanobenzoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Immunology 2022Quote: ... Spleens were disrupted using a syringe in 2% FBS/PBS and passed through a 40 μm nylon cell strainer (BD, catalog no. 352340) to obtain a single-cell suspension ...
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bioRxiv - Microbiology 2023Quote: ... which were grown in solid Lysogeny broth (LB) medium (5.0 g L−1 yeast extract, 10 g L−1 Bacto tryptone, 2 % Bacto agar (BD Biosciences, Franklin Lakes, NJ), 5.0 g L−1 NaCl ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences, 557735], and anti-CD107a [BD Biosciences, 560647]) by 20 minutes of incubation at room temperature ...
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bioRxiv - Pathology 2023Quote: ... Cells were diluted in basal medium containing 2% dextran-coated charcoal-stripped serum (DCC) (HyClone Laboratories, Logan, UT) and plated into culture dishes (BD Falcon, Bedford, MA). Cells were cultured for 24 hours in an incubator at 37°C with saturating humidity and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... digested cells were resuspended in FACS buffer (2% FBS–PBS) and analyzed by a FACS Calibur flow cytometer (Becton, Dickinson and Company, USA). Compensation and analysis of results were performed using Flow Jo software.
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed for 10 min in 2% paraformaldehyde and were analyzed using a FACS CANTO II (BD Biosciences, Franklin Lakes, NJ, USA). For lentiviral transduction efficiency assessment ...
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bioRxiv - Immunology 2023Quote: ... Naïve T cells were resuspended in complete medium (KOHJIN BIO DMEM-H (16003016) + 10% FBS) and activated in the presence of 2 μg/mL of anti-CD3 (BD Pharmingen, REF: 553058) and 2 μg/mL of anti-CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... 250,000-350,000 donor cells or the same volume of empty HBSS+2% FBS vehicle were intraperitoneally (IP) injected using a 30 G insulin syringe (BD Biosciences cat# 328431). Transplanted cells were allowed 2 weeks to engraft prior to SCI.
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bioRxiv - Neuroscience 2024Quote: ... Cell counting was performed in a hemocytometer and 2 million cells from each spleen were used for immunostaining and analysis in a flow cytometer (BD LSRFortessaTM X-20).
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bioRxiv - Immunology 2024Quote: ... 2×106 cells were stained as described above and sorted using a BD FACSAria II flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). In the control group ...
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bioRxiv - Biochemistry 2024Quote: Wild type RPE1 cells or RanGAP1ΔNES clones 2 and 10 were grown on 10 cm plates to 40-70% confluence and pulsed with 10 µM BrdU (BD Pharmigen flow kit) for 45 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD, Franklin Lakes, NJ, USA, cat.: 562440), anti-human CD20 (BioLegend ...
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bioRxiv - Developmental Biology 2021Quote: Mouse ESCs were pre-differentiated to an epiblast-like state as previously described [2] by seeding fibronectin-coated dishes (BD Biosciences cat. no. 356008) at a density of 0.8× 104 cells per cm2 in NDiff227 media (Takara cat ...
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bioRxiv - Neuroscience 2020Quote: ... and plated as dissociated cells (25 × 104 cells/well) on day −2 in a 6-well Matrigel-coated plate (BD Biosciences, San Jose, CA). On day −1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS 2% FBS and analyzed by flow cytometry using a FACSCanto and Novocyte flow cytometer (BD Biosciences and Acea Biosciences). The population of interest was gated according to its FSC/SSC criteria ...
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bioRxiv - Developmental Biology 2020Quote: Cryopreserved cells were thawed at room temperature and washed twice with 10 ml of FACS buffer (PBS and 2% FBS) and filtered through a 100 µM filter (BD BioSciences, cat no: 352360). Cell concentration was determined by hemocytometer ...
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bioRxiv - Immunology 2020Quote: 2 × 106 cells were stimulated for 4 h at 23°C with 40 ng ml−1 PMA and 1 μg ml−1 ionomycin in the presence of 2 μM Monensin (Golgi-Stop, BD Biosciences, Cat: 51-2092KZ). Cells were washed twice with FACS buffer and stained for extracellular markers ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
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bioRxiv - Immunology 2022Quote: Total white blood cells from 1-2 mL freshly collected blood in EDTA tubes were enriched through ammonium chloride lysis (BD Bioscience, catalog no. 555899), followed by centrifugation (300x g for 10min) ...
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bioRxiv - Biochemistry 2022Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed using 2% paraformaldehyde (PFA) solution for 30 minutes and permeabilization was performed using a 1X BD perm/wash solution (BD Biosciences, USA; cat#554723) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: All mycobacterial strains (Supplementary File 2 – Key Reagents) were grown in liquid culture containing Difco™ Middlebrook 7H9 Broth (BD Biosciences, San Jose, CA) and supplemented with 0.2 % (v/v ...
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bioRxiv - Immunology 2020Quote: ... Cells were collected from wells by pipetting with PBS (containing 2%FBS and 2mM EDTA) and data acquisition was performed on a BD Accuri C6 (Becton, Dickinson and Company, USA). All flow cytometry data were analyzed with FlowJo v10.1 (BD Biosciences).
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bioRxiv - Microbiology 2022Quote: ... After 19h incubation at 37°C, the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity (MFI ...
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bioRxiv - Neuroscience 2022Quote: ... approximately 1-2 mm apart along the same bundle of muscle fibers with the help of a 20G syringe tip (BD Microlance, Thermo Fisher Scientific). After the dental cement hardened ...
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bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
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bioRxiv - Microbiology 2024Quote: ... Cells were enumerated using a Cellometer X2 cell counter (Nexcelom Bioscience, Lawrence, MA, USA) and resuspended in yeast nitrogen base supplemented with 2% glucose (Difco; BD, Franklin Lakes, NJ, USA), to obtain a concentration of 2.0 × 106 cells/ml ...
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bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial strains were prepared for agar overlay plating by streaking out from glycerol stocks onto 2% streak agar plates (BD Difco 2216 marine broth), and allowed to grow for one days at room temperature ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended in PBS/2% FBS for analysis on a five-laser flow cytometer (FACSymphony A3 Cell Analyser, BD Bioscience, San Jose, CA, USA). Data were analyzed with FlowJo software (TreeStar ...
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bioRxiv - Neuroscience 2020Quote: Neurons from 18-day-old rat embryos were dissected and dissociated using trypsin and plated at a density of 70,000 cells cm−2 in minimal essential medium (MEM) supplemented with 10% NU serum (BD Biosciences, Le Pont de Claix, France), 0.45% glucose ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at room temperature followed by overnight incubation at 37°C in antibodies to the following: (1) C-terminal binding protein 2 (mouse anti-CtBP2; BD Biosciences, used at 1:200). (2 ...
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bioRxiv - Immunology 2021Quote: ... and intracellular cytokines (PE anti-mouse IFN-γ, PE-Cy®7 anti-mouse TNF-α, APC anti-mouse IL-2, all from BD Biosciences, USA). The labeled cells were acquired with the BD FACSCanto™ II flow cytometer and analyzed with the program FlowJo v.10.6.2 (BD Biosciences).
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bioRxiv - Microbiology 2021Quote: The actinobacteria were routinely cultured from frozen spore preparations via inoculation into Difco International Streptomyces Project (ISP) medium 2 (ISP2) (Becton, Dickinson and Company, Spark, MD) and incubated at 28° C ...
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bioRxiv - Immunology 2020Quote: ... before being washed and re-suspended in PBS/2% FCS for analysis on a five-laser flow cytometer (LSR FortessaTM; BD Bioscience, San Jose, CA, USA). The data were analysed with FlowJo software (TreeStar ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Pathology 2021Quote: ... SYN303 [aa 2-12 Cat# 82401, Biolegend], 1:500, non-amyloid ß component directed Syn-1 [aa91-99, Cat# 610787, BD Trasduction Laboratories], 1:500), Histone H3 ([Cat# 3638S ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (BD Pharmingen 553673 2 μg/ml) at 4 °C for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... BD 560046. After washing twice, cells were fixed in PFA 2% for 20 min, washed, and acquired on a Fortessa BD LSRII Cell Analyzer (Becton Dickinson).
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bioRxiv - Molecular Biology 2023Quote: ... PMVECs were purified after two rounds of immune selection using magnetic beads coated with anti-CD31 and anti-ICAM-2 antibodies (BD Bioscience, Franklin Lakes, NJ, USA). mPMVECs were cultured and maintained in a medium containing 5 % FBS and EC growth supplement (ECGS ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then washed 3x in flow cytometry stain buffer and fixed with 2% PFA for 20 minutes prior to acquisition on a LSRII Fortessa (BD Biosciences, San Jose, CA, USA). Samples were analyzed with FlowJo V10 software (Figure S4).
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bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...