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Citations for Becton, Dickinson and Company :
201 - 240 of 240 citations for PTP alpha Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Spleen cells of immunized mice were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 mAb (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Microbiology 2019Quote: ... or with stimulation by a total PCS peptide pool (PCS1-12, Table S11) were transferred to 12 × 75mm3 polystyrene tubes (BD Falcon) and washed with FACS wash (PBS supplemented with 2% FBS) ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Immunology 2020Quote: ... lung T cells were incubated at 37°C for 5 hours with 5 μM Bl-Eng2 or calnexin peptide and 1μg anti-mouse CD28 (BD, cat 553294). After 1h ...
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bioRxiv - Immunology 2020Quote: ... splenocytes and tumor-infiltrating cells were cocultured with 1 μmol/L of AdpgkMut or AH1 peptide in the presence of Brefeldin A (BD Biosciences) for 5h in vitro ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
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bioRxiv - Immunology 2020Quote: ... stimulated with 1 μg/ml LCMV NP118-126 peptide for 1 hour and then in the presence of GolgiPlug (BD Biosciences) and peptide for an additional 4 hours ...
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bioRxiv - Immunology 2021Quote: ... lung T cells were incubated at 37°C for 5 hours with 5 µM Bl-Eng2 peptide and 1µg antimouse CD28 (BD, cat 553294). After 1h ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with conjugated antibodies for 1 hour on ice: mouse anti-C-peptide antibody conjugated with Alexa Fluor 647 (BD Biosciences) and mouse anti-glucagon antibody conjugated with Bv421 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in sorting buffer (2% FBS and 1% Penicillin-Streptomycin in DMEM) and blocked with human FcR blocking reagent (Cat#564220; BD; used 1:50) for 10 minutes at 4 °C ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... IdU and CldU were then detected with the following primary antibodies in blocking solution for 1 hour at room temperature: mouse anti-BrdU (B44) (1:25) (BD Biosciences cat#347580) for IdU and rat anti-BrdU [Bu1/75 (ICR1)] (1:25 ...
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bioRxiv - Immunology 2021Quote: ... samples were stained with the relevant combination of surface and intracellular antibodies in combination with Fc blocking antibody CD16/CD32 (BD Pharmingen, Cat. #553141). BV421 anti-MHCII(IA/IE ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5-μm sections were stained with the following antibodies following citrate antigen retrieval and blocking with 10% normal donkey serum: mouse anti-glutamine synthetase (BD Bioscience, 1:2000), mouse anti-Ki67 (BD ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each pool) and brefeldin A (1 μg/ml; BD Pharmingen, USA) at 37°C for 12-16 hours ...
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bioRxiv - Microbiology 2020Quote: ... cells were restimulated for 5 hours with the same peptide pool in the presence of brefeldin A (BD Biosciences, San Diego, CA). Cells were then tested for IFN-γ ...
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bioRxiv - Cell Biology 2021Quote: ... C-peptide and Glucagon labelled cells were detected on an LSRII flow cytometer with FACSDiva™ Software (BD Biosciences, San Jose, CA) using the 405- ...
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bioRxiv - Immunology 2020Quote: ... up to 5×106 leukocytes were incubated with 5 μg/mL E6 peptide or 1 μg/mL α-CD3e (clone 145-2C11, BD Biosciences) in the presence of 1:1000 Golgi-plug for 5 hours ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug™, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 monoclonal antibody (mAb ...
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bioRxiv - Immunology 2020Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop™, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was done by standard techniques following 5-hour stimulation with Gp61-80 peptide and Brefeldin A (GolgiPlug™, BD Biosciences). No peptide controls were treated under the same conditions supplemented with Brefeldin A but without Gp61-80 peptide ...
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bioRxiv - Immunology 2020Quote: The following rat anti-mouse antibodies were used for leukocyte analysis within each tissue: purified CD16/CD32 Fc blocking antibody (clone 2.4G2; BD Pharmingen™, San Jose, CA), PerCP-Cy™5.5 Ly6G antibody (clone 1A8 ...
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bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed in 4% paraformaldehyde and permeabilized using a commercially available saponin-based permeabilization and blocking reagent (1x BD Perm/Wash buffer #554723). Cells were then stained using primary antibodies against Flag (Proteintech 20543-1-AP ...
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bioRxiv - Immunology 2019Quote: ... Mice were immunized subcutaneously with 50 μg of MOG35-55 peptide emulsified in complete Freund’s adjuvant (CFA, BD Difco, Franklin Lakes, NJ, USA) containing 500 μg of killed Mycobacterium tuberculosis (Strain H37a ...
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bioRxiv - Immunology 2020Quote: ... Isolated LNMCs were incubated with SIV peptide pools covering gag and env in the presence of co-stimulatory αCD28 and αCD49d (BD, 10 μg/mL) on goat-α-mouse IgG F(ab)2-coated (KPL ...
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bioRxiv - Immunology 2020Quote: ... and subsequently PBMCs were stimulated in R10 media with specific peptide pools or medium alone (DMSO control) in the presence of 1 μg/ml of α-CD28 (BD bioscience 555725) and α-CD49d (BD bioscience 555501 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated with fluorochrome-labeled monoclonal antibodies after blocking the Fc receptor with anti-CD16/32 monoclonal antibodies (BD Pharmingen, San Diego, CA. USA). Data were analyzed with FlowJo or Kaluza software ...
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bioRxiv - Neuroscience 2020Quote: EAE was induced in 7-week-old female C57BL/6J mice by subcutaneous flank administration of 200 μg MOG35-55 peptide emulsified with complete Freund’s adjuvant (CFA) (MOG35-55/CFA) (BD Biosciences, San Jose, CA, USA) supplemented with inactive ...
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bioRxiv - Microbiology 2019Quote: ... ESAT6 peptide (1 μg/ml) or TB10 peptide (1 μg/ml) for 24 hours at 37°C before the addition of GolgiPlug (BD Pharmingen, San Diego, CA). The concentration of protein and peptides used were according to previous reports [19 ...
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bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...