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Citations for Becton, Dickinson and Company :
201 - 250 of 4284 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The filter membranes with 8 μm pores and 0.33 cm2 were coated with 100 μL of 0.250 mg/mL Matrigel (BD Biosciences, USA) at 37°C / 5% CO2 for 2 h ...
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bioRxiv - Immunology 2024Quote: ... Intracellular of total (nuclear and cytosolic) TCF-1 and NFATc1 stainings were performed with anti-TCF-1 AF647 (S33-966, BD Pharmingen) and anti-NFATc1 PE (7A6 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred to supported nitrocellulose membrane and probed using Syn101 antibodies against aSyn (BD labs, San Jose, CA). Blots were imaged on using a ChemiDoc MP imager and analyzed using Image Lab (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... The following primary antibodies detected the proteins of interest: mouse anti-α-CaMKII (1:3000; BD, NJ, USA; 611292); rabbit anti-α-actin (1:3000 ...
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bioRxiv - Immunology 2021Quote: ... Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD), streptavidin-allophycocyanin-Cy7 (554063 ...
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bioRxiv - Cancer Biology 2023Quote: ... Surface or total MPL proteins were stained with anti- human MPL-PE antibody (BD Pharmingen 562159; 1:50 dilution).
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bioRxiv - Cell Biology 2024Quote: ... the total protein was detected by using specific antibodies (AP2: mouse anti-AP2 μ2, BD Biosciences, 611351, (1:250), Actin ...
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bioRxiv - Microbiology 2023Quote: PBS-washed cells were immunostained for individual surface proteins using the following antibodies: anti-BST2/Tetherin-BV421 (#566381; BD), anti-CCR5/CD195-FITC (#555992 ...
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bioRxiv - Immunology 2022Quote: ... Cells incubated with biotinylated monoclonal antibodies were incubated with fluorochrome-conjugated streptavidin–peridinin chlorophyll protein–cyanine 5.5 (551419; BD), streptavidin-allophycocyanin-Cy7 (554063 ...
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bioRxiv - Molecular Biology 2024Quote: ... We stained intracellular proteins using the following antibodies: anti-mouse CD3 BUV737 (1:100, 17A2, BD Biosciences, Cat. 612803) and anti-mouse CD4 Alexa Fluor 700 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... We processed the membrane for protein detection using antibodies against CD63 (1:1000; BD Biosciences, San Jose, California, USA), CD81 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... in flotation for 1 h at room temperature and then overnight at 4°C with the mouse antibody that recognizes protein GM130 (Cat# A-610822, RRID:AB_398141, BD Biosciences), followed by incubation for 2 h at room temperature with Alexa Fluor 546-conjugated goat anti-mouse secondary antibodies (Cat#A-11030 ...
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bioRxiv - Bioengineering 2021Quote: ... Dye dilution was determined after 5 days on an LSR II flow cytometer (BD). IFNγ concentrations in cell culture supernatants were determined after 48h by ELISA (Biolegend # 430104 ...
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bioRxiv - Immunology 2022Quote: ... BV staining buffer was used when multiple BV dyes were used together (BD Biosciences). The Foxp3 staining kit was used for intracellular transcription factor staining and the Cytoperm/Cytofix Plus staining kit was used for cytokine staining (BD Biosciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... Dilution of cell proliferation dye was evaluated by flow cytometry (LSRII Fortessa, BD Biosciences).
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bioRxiv - Immunology 2024Quote: CD3+ T-cell were labeled with 1μM Cell TraceTM CFSE dye (BD Biosciences, USA) for 20 minutes at 37°C and followed by activation with PHA (1-10μg/ml ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T-cell was labeled with 1µM Cell TraceTM CFSE dye (BD Biosciences, USA) for 20 minutes at 37C and followed by activation of CD3+ T-cell with PHA (1µg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... 5×104 cells in serum free DMEM were introduced to the upper chamber of an 8 μm pore size insert (BD Falcon), and DMEM medium supplemented with 10% fetal bovine serum was used as a chemoattractant in the lower chamber ...
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bioRxiv - Biochemistry 2020Quote: ... France) were differentiated on filter membranes (Individual Cell Culture Inserts with 0.4 μm pore size, BD Biosciences, San Jose, CA, USA) [22 ...
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bioRxiv - Cancer Biology 2021Quote: ... transfected/Dox treated stable cells (5×105 cells) in serum-free media were seeded in the upper chamber of an insert (8 mm pore size; BD Bioscience) coated with Fibronectin (BD Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was again discarded and pellets suspended in 300 µL of 0.02 µm-pore filtered before being transferred to BD Trucount™ Tubes (BD Biosciences) and proceeded to Flow Cytometry analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5×10^4 cells resuspended in serum free media was then added to the top 8 µm pore matrigel coated (invasion) or non-coated (migration) trans-well insert (BD Biosciences). After 20 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and plated on the upper chamber of the 24-well format HTS FluoBlokTM Multiwell Insert System (3 μm pore size; BD Falcon) in RPMI without phenol red (Life Technologies ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Individual spleens were removed and mechanically dissociated using a syringe plunger above 70 μm-pore size Falcon cell strainer (BD Biosciences). Red blood cells were lysed using ACK lysing buffer (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... the cell invasion assay was performed using Transwells (8 μm pore size, millipore) with inserts coated with Matrigel (50 mg/ mL, BD Biosciences). 786-O and ACHN cells (1.0 × 105 cells/well ...
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bioRxiv - Cancer Biology 2024Quote: ... A 30min permeabilization step using True-Nuclear™ Transcription Factor Buffer Set (424401) was performed before staining for SOX2 (561610, BD Biosciences) during 60min ...
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bioRxiv - Immunology 2020Quote: ... CLTX-CAR T cells were incubated with GBM cells (E:T=1:1) for 5 h in the presence of CD107a antibody and GolgistopTM protein transport inhibitor (BD Biosciences). To test for CAR T cell killing potency ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Cancer Biology 2020Quote: ... and knockout success was examined by Sanger sequencing and Western blot to confirm protein loss (anti-CDH1 antibody, BD #610182). 8 clones with the least E-cadherin protein expression by immunoblot were then pooled in equal ratio and named T47D CDH1 KO ...
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bioRxiv - Genomics 2020Quote: ... Primary antibodies (anti-Cas9 7A9-3A3, Cell Signaling Technology #14697S, anti-γ-tubulin Sigma#T5326, anti-Human Retinoblastoma protein BD Pharmigen #554136 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with antibodies specific for intracellular proteins (Supplemental Table 1) (1:100,v/v) in Perm/Wash buffer (BD) supplemented to contain 2% rat serum at RT for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... the cell suspension was stained with a LIVE/DEAD fluorescent dye (7-AAD; BD Biosciences) for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Proliferation was analyzed using CFSE and CellTrace Violet dye dilution on the LSRFortessa (BD Biosciences) and FlowJo.
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Asymmetric Binomial Statistics Explains Organelle Partitioning Variance in Cancer Cell ProliferationbioRxiv - Systems Biology 2021Quote: Jurkat cells labeled with dyes were sorted using a FACSAriaIII (Becton Dickinson, BD Biosciences, USA) equipped with Near UV 375nm ...
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bioRxiv - Immunology 2021Quote: ... PBMC were thawed and labeled using Violet Proliferation Dye-450 (BD Biosciences, San Jose, CA) and combined with DCs at a 1:4 ratio ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded from the analysis using fixable viability dye (FVD) BV421 (BD Biosciences). To assess the expression of cytokines ...
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bioRxiv - Cancer Biology 2024Quote: ... and hematopoietic cells stained with Fixable viability dye eFluor 455 (BD Biosciences 65-0868-14), murine Fc block (Bio X Cell BE0307) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 cells were added to the top chamber of 24-well transwells (8 μm pore size membrane; BD Bioscience, Bedford, MA), and assay medium was added to the bottom chambers and incubated for 24 hours (10% FBS) ...
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bioRxiv - Cancer Biology 2022Quote: Trans-well assay was done in 24-well Boyden chambers with 8-μm pore size polycarbonate membranes (BD Biosciences, San Diego, USA). ST1 cells (5 × 104 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 were added to the top chamber of 24-well Boyden chambers (8 μm pore size membrane; BD Bioscience, Bedford, MA), and medium was added to the bottom chambers and incubated for 24 hours (DMEM 10% FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... 1.5 × 105 cells were plated in the top wells of Growth Factor Reduced Matrigel-coated invasion chambers (8 μm pore size, BD Bio Coat). Media containing 5% was added to the lower chamber ...
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bioRxiv - Cancer Biology 2020Quote: ... and cell invasion was investigated by using 24-well transwell inserts that had been precoated with matrigel with 8 μm pores (cat No. 354480, BD Biosciences, USA). A total of 1×105 cells from the indicated treatments were cultured in serum-free medium in the upper chamber ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. Surface expression level of S proteins (Extended Data Fig. 6a) was measured using FACS Canto II (BD Biosciences) and the data were analysed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Immunology 2022Quote: PBS-washed cells were PFA-fixed and immunostained for individual surface protein expression using the following antibodies: Anti-CD3-FITC (#561807; BD Biosciences), anti-CD4-APC (#555349 ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Immunology 2020Quote: PBMCs or purified T cells were stained for 30 minutes on ice with the antibodies specific for extracellular proteins in Brilliant Stain Buffer (BD, 563794). Following incubation ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...