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Citations for Becton, Dickinson and Company :
201 - 250 of 4942 citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-β2 mAb IB4 (10 μg/ml) followed by Alexa Fluor 488-conjugated secondary antibody and analyzed using a FACScan (BD Biosciences) and Attune NxT flow cytometer (ThermoFisher) ...
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bioRxiv - Physiology 2024Quote: ... endothelial cells were purified by two consecutive immuno-selection procedures using magnetic beads conjugated with anti-ICAM-2 antibody (BD Pharmingen) and plated into 75 cm2 tissue culture coated flasks ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were blocked with 2% normal mouse serum and an anti-CD16/CD32 antibody (FcγR blocker, BD Pharmingen™ 553142) for 30 min on ice ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Immunology 2023Quote: ... 0.25X and 4X concentration of CD4 T cell-depleting antibody for 30 minutes at 37°C before surface staining with CD3 (clone SP34-2, BD Pharmingen), CD4 (Clone L200 ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with serum (2 fold dilutions, 1:20-1:2560) and parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... TCR expressing cells were mixed with K562-A2 cells at a ratio of 1:2 (Effector:Target) in the RPMI media and supplemented with 1 µg/ml of anti-CD28/CD49d antibodies (BD Biosciences, 347690) and 1 µg/ml of cognate peptides ...
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bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
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bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were assayed for IL-2 using capture and biotin-labeled detection antibody pairs (BD Biosciences, Franklin Lakes, NJ). Detection was performed with HRP-conjugated streptavidin (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cells were further stained intracellularly with antibodies against anti-human CD3 (APC-Cy7, clone: SP34-2, BD, cat# 557757, 1:200), anti-human IFNγ (AF700 ...
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bioRxiv - Immunology 2024Quote: ... and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM ethylenediaminetetraacetic acid (EDTA) buffer with 10% Brilliant Stain Buffer Plus (BD, 566385) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... 8 color (4-2-2) configuration (BD Bioscience, Heidelberg, Germany). The system automatically adjusts spillover values for the compensation of standard fluorochromes ...
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bioRxiv - Genetics 2021Quote: ... 2% peptone (BD), and 2% dextrose ...
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bioRxiv - Microbiology 2021Quote: ... 2% (BD Biosciences). Recovery plates (R0 ...
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bioRxiv - Neuroscience 2024Quote: ... β2 Spectrin (BD Biosciences Cat# 612563 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and HRP-conjugated goat anti-mouse IgG (1:10,000; BD Biosciences Cat # 554002) was added ...
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bioRxiv - Immunology 2021Quote: ... HRP-bound Streptavidin was detected by adding 50 μl/well TMB substrate (BD Bioscience ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed and then incubated with the streptavidin-conjugated HRP (BD) for 1h at RT ...
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bioRxiv - Immunology 2022Quote: ... a 1:2000 dilution of HRP Goat Anti-Mouse Ig (BD, cat. #554002) diluted in PBS with 1% BSA was added to the plate and incubated for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and HRP-conjugated streptavidin (1:1,000; BD Biosciences, Cat. 554066) in blocking buffer was added for 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed 3× with PBST and 50 μl Streptavidin-HRP (BD Biosciences) diluted 1:100 in dilution buffer was added to the wells and incubated for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated goat anti-rabbit and anti-mouse (BD Pharmingen #554021 and #554002). Alexa Fluor 568- and 488-conjugated antibodies were purchased from Life Technologies and used at a 1:1000 dilution.
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used for immunofluorescence of HeLa cells and/or primary neurons were: mouse anti-Ataxin-2 (BD Biosciences 611378, 1:250), mouse anti-beta-III-tubulin (Covance MMS-435P ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Permeabilization buffer (eBioscience) and incubated for 15 min in FACS buffer (PBS, 2% FCS) before adding the primary MASH-1 antibody (BD Biosciences, 556604), 1:250 ...
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bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Biophysics 2020Quote: Spectrin in neurons (Figure 2d and Supplementary Figures 2 and 5) was labelled with a mouse monoclonal primary antibody anti-β-Spectrin II (Clone 42/B-Spectrin II, BD Biosciences) for 1 h at room temperature using a 1:400 dilution in 5% BSA in PBS ...
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bioRxiv - Microbiology 2020Quote: ... 50 μL of EDTA-anticoagulated whole blood were stained with the monoclonal antibodies CD3 BV605 (SP34-2, BD Biosciences, San Jose, CA), CD4 PerCP/Cyanine5.5 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... then probed with the following primary antibodies in 5% milk/TBS-T overnight at 4 °C: SREBP-2 (1:250; BD Biosciences, 557037), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were blocked with 2% BSA in 0.1% tween TBS (TBST) for 1h and immunostained with mouse anti-paxillin IgG1 antibody (BD Transduction Laboratories 610052) (1:500 ...
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bioRxiv - Immunology 2024Quote: ... and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD, Cat#566385) for 30 min on ice ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with AF647-anti-VE-cadherin antibody (1:200, v/v, diluted in pre-warmed EGM-2 media, BD Biosciences, #561567) for 30 minutes orbital shaking ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of IL-2 (BD OptiEIA Human IL-2 kit) and IFN-γ (BD OptiEIA Human IFN-γ kit ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation for 2 hours at room temperature with primary antibody (SOX2 [1:200, EMD Millipore AB5603], NESTIN [1:200, BD Pharmingen #556309]). After washing five times with PBS/0.05% Tween-20 ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed three times with PBST before adding Streptavidin-HRP (BD Bioscience). The BD ELISPOT AEC substrate (BD Bioscience ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed three times with PBST before adding Streptavidin-HRP (BD Bioscience). The BD ELISPOT AEC substrate (BD Bioscience ...
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bioRxiv - Immunology 2023Quote: ... Responses were visualized by adding HRP anti-human IgG (BD Pharmingen, San Diego, California) followed by o-Phenylenediamine dihydrochloride (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... anti-CD3 BV605 (clone SP34-2; 2 μL; cat# 562994) from BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... interleukin-2 (IL-2) and tumor necrosis factor α (TNF-α) (BD). All samples were tested with BD FACS Canton flow cytometer (BD).
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bioRxiv - Cell Biology 2020Quote: ... 2% Bacto-peptone (BD), 0.025% adenine hemi-sulfate (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... 557998)/IL-2 (BD, 560547)/IL-4 (BD ...
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bioRxiv - Immunology 2022Quote: ... 2% Fc block (BD), and fixable viability dye eFluor 450 (ebiosciences) ...