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Citations for Becton, Dickinson and Company :
201 - 250 of 6565 citations for 4 1 Hydroxymethyl cyclopropyl phenylboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... with 0.2% Triton X-100 supplemented with 10% FBS and then incubated overnight at 4 °C with primary antibodies (1:100 mouse anti-ASCL1, BD 556604 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary (overnight at 4 °C) and secondary (1h at room temperature) antibodies (1:100 mouse anti-ASCL1, BD 556604 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with one of four antibody panels (Supplementary Table 1) assessing different immune populations and analysed by flow cytometry (4-laser LSR Fortessa, BD). Raw FCS files were analysed using FlowJo (v10.6.2 ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells were blocked for 20 min at 4°C with FcR antibody (purified anti-CD16/32; BD Pharmingen) diluted (1:50 ...
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bioRxiv - Pathology 2020Quote: ... slides were incubated overnight at 4 °C with a 1:100 dilution of N-cadherin antibody (BD Transduction Labs, #610920) followed by incubation with secondary fluorescence antibody Alexa Fluor Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Cryosections were then incubated overnight at 4°C with primary antibody rat anti-mouse CD31 (BD PharMingen, PMG550274; 1:50). The next day ...
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bioRxiv - Immunology 2020Quote: ... media for 1 hour at 37°C and then an additional 4 hours with Brefeldin A (10 μg/mL) and GolgiStop (BD) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were probed overnight at 4°C with the following primary antibodies: anti-ALIX (AIP1) (1:250; BD Biosciences, 611621), anti-CD9 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... diluted 1:1000 in PBS for 30 min at 4 °C and stained with PE Rat Anti-Mouse CD45 antibody (BD) in FACS buffer for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... cell suspensions were surface labelled for 30 min at 4°C with CD3 BV785 (clone 17A2, 1:50, BD Horizon), CD8α BUV805 (clone 53-6.7 ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were stored in PBS at 4°C for up to 1 week in dark before flow cytometry analysis by LSRFortessa (BD). The data were analyzed by the WEASEL software (2.7 ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated overnight at 4° with primary antibodies at the following dilutions: mouse anti-βII-spectrin 1:2000 (BD-bioscience), rabbit anti-βII-spectrin 1:2000 (Abcam) ...
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bioRxiv - Cell Biology 2023Quote: ... CECs were incubated overnight at 4°C with primary antibodies mouse monoclonal anti-CD166 [3A6] (1:200 dilution, BD Biosciences), rabbit polyclonal anti-ZO1 (1:100 dilution ...
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bioRxiv - Immunology 2023Quote: ... and 3 μg/ml anti-IL-4 in the presence of plate bound anti-CD3ε (1 μg/ml) (BD Biosciences) and anti-CD28 (3 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) and analyzed on a BD FACS Aria III (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... High-titer viral solutions were prepared and used for transduction into both WT and NF1 KO BT-474 cells by two rounds of infection of 4 viral particles per cell (MOI 1) followed by flow cytometry-based sorting (FACSAria cell sorter, BD). For live cell imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... were coated overnight at 4 °C with 300 ng/mL mouse anti-human RIPK1 capture antibody (1:100 dilution in PBS of BD Bioscience BD610459 lot 0314064 ...
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bioRxiv - Immunology 2023Quote: ... Surface antigens were stained for 30 min at 4°C with indicated antibodies (Table.1) after Fc blocking for 4°C 30 min with anti-mouse CD16/32 (BD) without wash ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated for 15 minutes at 4°C with anti CD16/CD32 Mouse BD Fc Block diluted 1/50 (BD) in 100 µL of FACS buffer in 96 wells round-bottom plates (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 5 min at 4 °C and immunostained with CD11b-AlexaFluor 647 (M1/70; 1:1000; Cat#557686, BD Biosciences) and CD45-PE (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were incubated overnight at 4°C with primary antibodies: Mouse monoclonal anti-BrdU (1:100, BD Biosciences, 347580) and Rat monoclonal anti-BrdU (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Washing steps between incubations (400 × g, 4 min, 4 °C) were done with Cell Wash (BD Biosciences). Prior to staining ...
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bioRxiv - Molecular Biology 2021Quote: ... and supplemented with Yeast Nitrogen Base containing ammonium sulfate (without amino acids, BD), bacto agar (2% w/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... containing 1.7 g/L yeast nitrogen base (YNB) without amino acids (BD Biosciences), 5 g/L ammonium sulfate (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The blood samples were collected in ethylenediaminetetraacetic acid (EDTA) Vacutainer tube (BD, 0202992058) and stored at RT (18-22◻ ...
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bioRxiv - Immunology 2021Quote: ... Blood was collected into Ethylenediamine tetra-acetic acid (EDTA) coated tubes (BD Vacutainer).
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bioRxiv - Immunology 2021Quote: ... and surface stained for 30 mins at 4°C with 50 μL/well anti-mouse CD3 APC-CY7 (clone 17A2, 1:400, BD Biosciences), anti-mouse CD4 BV510 (clone RM4-5 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C with the astrocyte (mouse anti-GFAP; 1:1000, BD Biosciences, San Diego, CA, USA) and microglia (rat anti-CD11b ...
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bioRxiv - Immunology 2021Quote: ... Bulk of primary cells (either from the spleen or the lungs) were then rechallenge in vitro with YAK-1 cells for 4 hours in presence of monensin and brefeldin (BD Bioscience) at the Effector/Target ratio of 5 ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was recovered and sequentially incubated with anti-mouse CD16/CD32 (1:250, BD Pharmingen; for 15min at 4°C) followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by rat anti-CD11b and PerCP-Cy5.5 rat anti-mouse CD45 (1:200, BD Pharmingen; for 30min at 4°C), all in 2% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Genomics 2021Quote: ... the suspension was sorted by applying a 4-way purity mode based on the selected DAPI positive nuclei population (1:1000, BD Pharmingen) using forward and side scatter gating strategy (FACS) ...
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bioRxiv - Microbiology 2020Quote: ... during 15 min at 4 °C in MACS buffer followed by incubation with anti-CD45 1:100 (clone 30F-11, BD biosciences), anti-EpCAM 1:100 (clone G8.8 ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 1% (vol/vol) formaldehyde for 30 min at 4°C and analyzed on an LSRFortessa (BD Biosciences) flow cytometer ...
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bioRxiv - Immunology 2021Quote: ... or GFP-Nb-Cy5.5 (∼51 nM) in 1% FPS/PBS at 4°C for 20 min and subsequently analyzed on a LSR-II cytometer (BD biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of IL-2 followed the same protocol as that for CTLA-4 but included the addition of 1 μg/mL GolgiPlug (BD Biosciences) to the culture media 12 hours prior to sample preparation ...
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bioRxiv - Immunology 2020Quote: The cell supernatant was collected after centrifugation of the cells and the secreted IL2 in the diluted cell culture medium (1:4 dilution) was measured using the IL2 Flex set cytometric bead array (CBA) (BD, 558270) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Cell Biology 2022Quote: Freshly dissected LW whole mount preparations from 6 wild-type and 4 Lrp2-/267 mice were incubated with rat anti-CD24 conjugated with Phycoerythrin (PE; 1:100; BD Pharmingen) in Neurobasal medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Cell Biology 2023Quote: Both WT and CD13KO mice of both genders were intraperitoneally injected with 1 mL 4% Brewers Thioglycollate (TG) solution (BD; 211716). After 48 hours mice were then euthanized via CO2 and cervical dislocation and injected with 10 mL cold PBS into the peritoneal cavity to collect peritoneal macrophages ...
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bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...