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Citations for Becton, Dickinson and Company :
2301 - 2350 of 3510 citations for Human Tropomyosin alpha 4 chain TPM4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: The cell supernatant was collected after centrifugation of the cells and the secreted IL2 in the diluted cell culture medium (1:4 dilution) was measured using the IL2 Flex set cytometric bead array (CBA) (BD, 558270) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 4% heat-inactivated FCS containing FACS buffer and data were acquired by a Symphony Flow Cytometer (BD Biosciences). Flow cytometry and t-SNE analyses were performed with FlowJo software (FlowJo10.5.3) ...
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bioRxiv - Immunology 2019Quote: Emigrated cells were washed in PBS with 1% BSA and 0.02% NaN3 and incubated for 30 minutes on 4°C with subset markers HLA-DR BV510 (clone G46-6, BD biosciences), CD1a APC (clone HI149 ...
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bioRxiv - Cancer Biology 2019Quote: Approximately 0.5-1 × 106 cells were stained for 15-30 minutes at 4°C in 96-well U-bottom plates (BD Biosciences) with directly conjugated antibodies (Table S8) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained overnight at 4°C in 1X perm/wash buffer with FITC mouse Ki67 set (BD Pharmingen #556026). Cells were then washed with 1X perm wash buffer and re-suspended in PBS supplemented with 1ug/ml 4′,6-diamidino-2-phenylindole
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bioRxiv - Cell Biology 2020Quote: ... Kidney sections were blocked (10% normal goat serum and 1% bovine serum albumin (BSA) in PBS) and incubated overnight at 4°C with primary antibody ((Ly6G, 1:10; BD/Pharmingen), FITC-C3 (1:200 ...
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bioRxiv - Genomics 2020Quote: ... Cells were then incubated with 5ul human Fc Block with 95ul 2% FBS in PBS at 4°C for 15 mins before staining with SCMK and AbSeq antibodies (BD Biosciences) at 4°C for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells with normal mCherry fluorescence (mid 70-80% fluorescence) and top or bottom ~7% eGFP fluorescence (~4 million cells/population) were sorted using a BD FACSAria III (BD Biosciences) and collected into 15 ml polypropylene tubes (Cellstar ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 50 nM phorbol-12-myristate-13-acetate (MilliporeSigma) and 250 nM ionomycin (MilliporeSigma) for 4 hours in the presence of Brefeldin A (BD Biosciences) before harvesting ...
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bioRxiv - Immunology 2020Quote: Cells isolated from the organs indicated in figure 4 and figure S9 were incubated with an Fc blocking agent (BD Pharminogen) for 10min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The cells (2-4 × 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibodies to either IFN-γ or IL-4 (BD), and were exposed to various stimuli (e.g ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Microbiology 2020Quote: ... cell debris pelleted by centrifugation (1400 x g for 10 min at 4°C) and supernatants assayed for chemokine and cytokine levels using the CBA flex set (BD Bioscience) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cells were stored overnight at 4°C and were then acquired on a Symphony flow cytometer with Diva software (BD Biosciences). A minimum of 5 × 104 events were acquired for each sample.
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bioRxiv - Immunology 2021Quote: ... cells were restimulated ex vivo with PMA (50 ng/ml) and ionomycin (750 ng/ml) for 4 h in the presence of GolgiPlug (BD Bioscience), and then fixed and permeabilised following intracellular staining with anti-mouse IL-17A (clone ReBio17B7 ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged and re-suspended in PBS and stored at 4°C prior to cytometric analysis using a using LSRFortessa flow analyzer (BD Biosciences). Results were analyzed using FlowJo V10 software (Flowjo ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for 30 min at 37°C in 1 µM JC-1 dye washed twice in PBS 4% FBS and analyzed on an LSR Fortessa X20 (BD Biosciences). Flow-Jo software was used for post-acquisition analysis ...
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bioRxiv - Immunology 2022Quote: ... The tube was then stored in the dark at 4° C for up to 2 hours prior to acquisition on the LSRII or FACSLyric analyzers (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: Freshly dissected LW whole mount preparations from 6 wild-type and 4 Lrp2-/267 mice were incubated with rat anti-CD24 conjugated with Phycoerythrin (PE; 1:100; BD Pharmingen) in Neurobasal medium (Gibco ...
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bioRxiv - Neuroscience 2022Quote: SH-SY5Y cells were seeded at a density of 4×103 cells/well in flat bottom 96-well plates previously coated with Matrigel (BD, Biosciences), and 10 μM all-trans-retinoic acid (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... stained on ice for 30’ with relevant cocktails of antibodies and populations of interest were sorted at 4°C on an BD FACSARIA (BD Bioscience) using a 70 μM nozzle in 50% FBS RPMI ...
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bioRxiv - Immunology 2022Quote: ... intracellular cytokine staining was performed at 4°C for 30 minutes using a cocktail of antibodies resuspended in 1x Perm/Wash buffer (BD Biosciences); intracellular antibodies included IFNγ APC-Cy7 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... cells were detached using 150 μl of PBS + 4 mM EDTA and analyzed via flow cytometry on a BD LSRFortessa Cell Analyzer (BD Biosciences). Transgene expression was detected in the PacBlue channel.
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Immunology 2022Quote: ... 20 μm paraformaldehyde fixed lung sections from Mtb-mCherry infected I-Tomcat Ai6 and Sp140−/− I-Tomcat Ai6 mice were stained at 4°C overnight with BV421-labeled SIRP□ (P84, BD Biosciences), Pacific Blue–labeled B220 (RA3-6B2 ...
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bioRxiv - Biochemistry 2022Quote: ... Salmonella from infected macrophages was fixed for 15 min in 4% PFA/PBS before analysis on a flow cytometer (Symphony, BD Biosciences) using thresholds on side scatter (SSC ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometry evaluation was performed the following day using a 4-laser (405 nm, 488 nm, 561 nm, 640 nm) LSRFortessa analyzer (BD instruments). The following antibodies and reagents were used for flow cytometry experiments ...
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bioRxiv - Immunology 2022Quote: ... Cells were then resuspended in FACS buffer (1% BSA in D-PBS) and kept at 4°C in dark until analyzed with a customized BD LSR Fortessa flow cytometer (BD Biosciences). Compensation was performed using ArC and UltraComp compensation beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Immunopurified Ngfrp75-positive mouse OECs were plated on PLL-coated 4-chamber polystrene-vessel culture slides (BD Falcon, San Jose, CA) or used for western blots ...
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bioRxiv - Immunology 2024Quote: ... surface antibodies were incubated with cells for 30 minutes at 4°C in hFACS buffer and Brilliant Stain Buffer (BD Biosciences). For intracellular staining ...
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bioRxiv - Neuroscience 2023Quote: ... cell suspension was incubated with Fc block antibodies for 5 minutes at 4 °C and then labeled with anti-mouse Thy1.2 magnetic particles (BD IMag™) for 30 minutes at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25% Triton PBS before incubation overnight at 4°C with the following antibodies: Rat monoclonal to mouse Sca1 (740450, BD Biosciences), Rabbit monoclonal to mouse SOX9 (ab185230 ...
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bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
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bioRxiv - Physiology 2024Quote: ... Small intestine lamina propria cells from male C57BL/6J mice were prepared and stained with surface molecules for 30 min at 4 followed by sorting on a FACS Aria III cell sorter (BD Biosciences). A gating strategy of live Lineage−CD127+KLRG1-c-Kit+ was used to sort the ILC3s.
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bioRxiv - Immunology 2024Quote: Live single CD45+TCRβ+CD4+IL10+ cells were sorted from isolated lamina propria cell suspension from three pooled Giardia-infected mice per sample (total of 4 samples) using a BD FACSAria Cell Sorter (BD Biosciences). Then ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with staining buffer, fixed, and permeabilized (20 min, 4°C, dark) using Fixation and Permeabilization Solution (BD Biosciences). Cells were then stained with S100A9 (Clone ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood chimerism was determined in recipients by blood collection via cheek bleeds every 4 weeks for 16 weeks and cells were analyzed by flow cytometry using the LSRII (BD Biosciences). Long-term multi-lineage reconstitution (LTMR ...
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bioRxiv - Cell Biology 2023Quote: ... and cells fixed in 4% paraformaldehyde (PFA) were analyzed on a Stratedigm (S1000EXI) cytometer with post- processing using FlowJo software (BD Biosciences). For all other analyses ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Immunology 2023Quote: ... for 20 mins at RT and then fixed samples were then resuspended in 100µL wash buffer prior to acquisition on the 4-laser BD® LSRII (BD Biosciences). Instrument setup and calibration were performed using EuroFlow standard operation procedures as described previously.43
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for 15 min at 4 °C with a purified mouse anti-rat CD32 FcγII (clone D34-485, Rat BD Fc Block) to block unspecific binding ...
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bioRxiv - Developmental Biology 2023Quote: MACS depleted non-hematopoietic cells were stained with the antibodies listed in Table S1 for 20 min at 4°C and analyzed on a spectral cytometer ID7000 (SONY) as described(4) or sorted with a BD FACS Aria III (BD Biosciences) according to the guidelines for the use of flow cytometry and cell sorting(5).
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bioRxiv - Developmental Biology 2023Quote: Hematopoietic cells were stained with the antibodies listed in Supp Table S1 for 20-30 min at 4°C and analyzed on a custom BD LSR Fortessa (BD Biosciences). For sorting ...
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bioRxiv - Biophysics 2023Quote: ... They were finally washed with 4 mL FACS buffer and re-suspended in 0.5 mL to be analyzed in a LSR Fortessa X20 (BD Biosciences, Europe). For live staining ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then fixed (eBioscience #00-8222-49) at 4 °C for 20 minutes and permeabilization (eBioscience #00-8333-56) before staining for intracellular IFNγ (BV650, BD #563854) and TNFα (BV421 ...
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bioRxiv - Microbiology 2023Quote: Stained cells were stored for up to 2 days at 4°C in the dark (TRAIL-R1) and flow cytometry was performed on a BD FACSCanto II (BD Biosciences). Raw data were exported to FlowJo v10.8.1 for analysis and plotting.