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Citations for Becton, Dickinson and Company :
2301 - 2350 of 8332 citations for 6 Chloro 2 methyl 1 2 4 triazolo 4 3 b pyridazin 3 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Human IFN-γ ELISA Set (555142) and Human IL-2 ELISA Set (555190) were purchased from BD OptEIA™ ...
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 RBD was multimerised with SAv-BV421 and SAv-PE (563259, 554061; BD Biosciences) individually ...
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bioRxiv - Biochemistry 2023Quote: ... pellets were resuspended in FACS buffer (PBS + 2% FCS) and analyzed by flow cytometry (BD LSRFortessa™) and FlowJo software.
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bioRxiv - Cell Biology 2023Quote: Fresh blood samples were collected from healthy volunteers in tubes containing EDTA 2 K tubes (BD Biosciences), WBCs were prepared by removing red blood cells using Lysing Buffer (BD Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... macrophages or granulocytes (CD14, Sa14-2-APC, #123311, BioLegend and CD11b, M1/70-APC, #553312, BD Biosciences) at a ratio of 1:10 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2022) (Liimatainen et al. 2022) including 22 sequences of 21 species (2 outgroup species, Crepidotus sp. BD-2015/Hebeloma cylindrosporum and 19 ingroup species ...
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bioRxiv - Microbiology 2024Quote: ... Cells isolated from ileum and mLN were additionally stained with anti-CD138 BB515 (281-2; BD Biosciences), anti-IgA BV786 (C10-1 ...
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bioRxiv - Biophysics 2021Quote: ... filled with motility buffer containing various concentrations of α-methyl-aspartate (MeAsp) through polyethylene tubing (Polythene Tubing, 0.58 mm id, 0.96 mm od; BD Intermedic). The tubing was connected to the PMDS device through stainless steel pins that were directly plugged into the inlets or outlet of the device (New England Tubing) ...
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bioRxiv - Immunology 2021Quote: ... Slides were treated for 10 minutes in H2O2 and after blocking in 10% goat serum in 0.1% Tween20 for 30 minutes and incubated overnight at 4°C with specific antibody diluted in PBS 0,1%Tween20: anti-mouse Ly6G (1A8, BD Pharmingen, cat 551459), or anti-mouse Ki67 (D3B5 ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were incubated with primary antibody solutions (using 5% milk or BSA in TBST) overnight at 4°C at the respective dilutions: E-Cadherin (BD, #610181,1:5000), Vimentin (Abcam ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were collected and fixed in 4% paraformaldehyde prior to acquisition on a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA). CFSE signal intensity of 1 × 104 cells was recorded and analyzed to measure cell divisions ...
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bioRxiv - Bioengineering 2020Quote: ... was applied for 30 minutes at 4°C and stained cells were resuspended in FACS buffer before flow analysis on a BD FACSCanto™ II (BD Biosciences). Gating and flow data were processed on FlowJo (FlowJo LLC).
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bioRxiv - Microbiology 2021Quote: ... and several ten-fold dilutions (10−4 to 10−7) were plated onto de Man Rogosa and Sharpe (MRS) media (BD Difco, USA) supplemented with 50 mg/L of mupirocin (PanReac AppliChem ...
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Molecular Biology 2020Quote: ... Then the syndecan-4 expression on HUVECs was measured by flow cytometry with a BD Biosciences LSR II (BD Biosciences, CA, USA).
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Immunology 2019Quote: Cells were harvested by centrifugation at 400 g for 5 minutes at 4°C followed by staining with fixable viability stain (BD Horizon FVS700) for 10 minutes on ice in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... A 1ml astrocyte cell suspension of 4×104 cells/ml was plated in three chambers of four-chamber glass slides (BD Falcon, USA) coated with poly-D-lysine ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2020Quote: ... in house production), 10 μg/mL α-IL-4 (cl. 11B11, in house production) and 10 ng/mL IL-12 (BD Pharmingen) and cultured on goat α-hamster IgG (MP Biochemicals ...
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bioRxiv - Immunology 2019Quote: ... Cells were resuspended in PBS/5%FBS then fixed in an equal volume of 1% paraformaldehyde and stored in the dark at 4°C prior to analysis on a BD LSR FortessaTM or LSR2 flow cytometer (BD Biosciences, UK). Data were analysed using FlowJo software (FlowJo LLC ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed at 4 °C for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 °C for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer ...
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bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cells were washed once in FACS buffer before fixation for 20 min at 4°C using the BD Cytofix/Cytoperm solution and permeabilization using BD Perm/Wash™ buffer (BD Biosciences). Permeabilized cells were incubated for 30 min with antibodies (diluted 1:200 in BD Perm/Wash ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... treatment was performed at 100μg/ml concentration in dark at 4°C for an hour and analyzed by FACS Calibur flow cytometer and processed using Cell Quest software (BD Biosciences, USA).
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL medium containing 1 × 106 luciferase-expressing MCF10AER/vSrc cells (control or 4-OHT transformed, with or without NLS-catalase expression) were mixed with 75 μL Matrigel matrix (BD Bioscience, #356237) containing 10 μM 4-OHT and injected into the inguinal mammary fat pad of the female mice at 6 to 8 weeks of age ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Genomics 2023Quote: Whole blood aliquots at a volume of four (4) milliliters were collected from participants using sterile Vacutainer blood collection kits (BD Vacutainer®) and subsequently biobanked at −80°C for DNA extraction ...
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bioRxiv - Immunology 2023Quote: ... Blood was collected via submandibular bleeds with goldenrod 4 mm lancets collected into sample tubes coated with K2 EDTA (BD Ref# 365974) and 60 μL of blood was lysed with 500 uL of ACK lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Cels were fixe with 4% PFA and stained with Rb anti-HA for NDST1 (CST 3724S) and Ms anti-GM130 (BD biosciences 610823) for Golgi followed by secondary antibodies staining with anti-Rb-A550 and anti-Ms-A647 ...
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bioRxiv - Immunology 2024Quote: ... followed by two washes with the permeabilization buffer before incubation with 4 μg/ml Alexa Fluor® 647 Mouse anti-Phospho-NF-κB p65 (pS529) (BD Biosciences) for 45 min.
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bioRxiv - Immunology 2024Quote: ... Surface staining was carried out for 30 minutes at 4°C with the following fluorochrome-labeled anti-mouse Abs: APC/Cy7-CD45 (BD Bioscience, #557659), PE/Cy5-B220 (eBioscience ...
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bioRxiv - Genomics 2020Quote: ... each anaesthetised snail was injected with 170 μL of snail saline (see Standen 1975) in the head-foot using a syringe and needle (0.45 mm × 16 mm BD Microlane™ 3, New Jersey, USA). This treatment examined the effect of wounding in activating immune defence when compared to anaesthesia without exposure to immune elicitors (treatment 2 above) ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... NP-FB23-2 (3 mg/kg) was delivered through the retro-orbital sinus into neonatal mice with a 31-gauge insulin syringe (BD Biosciences, San Jose, CA, USA).
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 1 h with primary antibodies to RAD51 (B-bridge International, #70-001) and to cyclin A (BD Biosciences, # 611268) diluted in 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were blocked with 5% donkey serum and 0.1% Triton X-100 in PBS for one hour at room temperature and incubated with 1:1000 of anti-CD47 (BD Biosciences), 1:500 of anti-TSP1 (Abcam) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then spun down and resuspended in 100LμL of buffer containing fluorescently conjugated antibodies for one hour (5LμL of each antibody per sample): APC-Cy7 Mouse Anti- Human CD4 (1:20 dilution; BD Biosciences, #557871), APC-Cy7 Mouse IgG1 ...
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bioRxiv - Genetics 2019Quote: ... the following antibodies were used (volume per 1×10^6 cells, all from BD Biosciences, unless stated otherwise): CD45RA FITC (5μl ...
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bioRxiv - Neuroscience 2024Quote: ... C06/C20 microglia were cultured at 1 x 106 cells per well in 6-well plates (BD Falcon), and iMicroglia were cultured at 5 x 104 cells per well in 96-well plates (CellBind) ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... DNA was denatured in 2 M hydrochloric acid and stained with FITC-conjugated BrdU antibody (556028, BD Biosciences) and propidium iodide (MilliporeSigma ...
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bioRxiv - Immunology 2019Quote: ... A minimum of 2×105 events within the lymphocyte population were acquired in a FACSCanto II (BD Biosciences) flow cytometer using FACS Diva Software (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed with 2% paraformaldehyde for 20 minutes before analysis on a FACSCanto II cytometer (BD biosciences). The antibodies used were Fas (Biolegend 305602 ...
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bioRxiv - Immunology 2021Quote: ... Red blood cells were removed by adding 2 ml of BD FACS Lysing Solution (BD Bioscience, Heidelberg, Germany) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 mM MgCl2) for another 20 min at room temperature before analysis by FACSAria II (BD Biosciences).
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed 2 x in FACS buffer and then incubated in Cytofix fixation buffer (BD Biosciences). Cells again washed in FACS buffer prior to performing flow cytometry using a BD Fortessa flow cytometer and data analysis was performed using FlowJo software (Tree Star).
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bioRxiv - Microbiology 2020Quote: ... Cells were subsequently surface stained with an antibody cocktail consisting of CD3 BV 650 (Sp34-2, BD Biosciences), CD4 BV605 (OKT4 ...