Labshake search
Citations for Becton, Dickinson and Company :
2251 - 2300 of 2538 citations for Human Neuropeptide FF NPFF CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Single-Cell capture was performed with BD Rhapsody as described above using Rhapsody Enhanced Cartridge Kit (BD Biosciences 664887). Reverse transcription from beads and sequencing library production was carried out according to manufacturer’s instructions (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... for the detection of cleaved Caspase 3 we used the PE active Caspase 3 apoptosis kit (550914, BD Biosciences) and for the detection of Annexin V we used the FITC Annexin V Kit (Immunostep) ...
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bioRxiv - Developmental Biology 2023Quote: ... Collected cells were washed with PBS and fixed and permeabilized using BD Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Biosciences). Cells were stained with Click-iT Plus OPP reaction cocktail for 30 min at room temperature and analyzed by FACS Symphony (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized following manufacturer protocol using Cytofix/Cytoperm™ Fixation/Permeabilization Kit (BD Biosciences, cat #554714). After centrifugation and washing ...
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bioRxiv - Immunology 2023Quote: ... followed by fixation and permeabilization using the Fixation/Permeabilization buffer kit according to manufacturer’s instructions (BD Biosciences, NJ, USA). This was followed by staining with anti-phospho-Akt antibody diluted in Perm/Wash buffer for 1h ...
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bioRxiv - Neuroscience 2023Quote: ... astrocytes and endothelial cells (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, ThermoFisher; CD45.1 clone A20 in BV421 (BD); CD45.2 clone 104 in BV421 (BD) ...
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bioRxiv - Cell Biology 2024Quote: ... bone marrow cells were stained using BD Stemflow™ Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit (BD, 560492) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... BD Rhapsody beads (“Enhanced Cell Capture Beads V2”, Part Number 700034960, BD RhapsodyTM Enhanced Cartridge Reagent Kit, BD 664887) were incubated with a splint ...
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bioRxiv - Immunology 2024Quote: ... Serum supernatant was collected and loaded onto BD Cytometric Bead Array Mouse Th1/Th2/Th17 CBA kit (BD Biosciences), as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... co-culture supernatants were thawed at room temperature for 1 hr and cytokines stained with mouse Th1/Th2/Th17 cytokine kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Cell quantities were quantified with flow cytometry using the BD Cell Viability Kit with BD Liquid Counting Beads (BD Biosciences). Cultures were then centrifuged at 3,000 g for 15 minutes and washed with sterile PBS three times before dilution into sterile water to a final concentration of 9x105 cfu/mL.
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bioRxiv - Immunology 2021Quote: ... fixed and stained with APC anti-BrdU antibody and 7-AAD using the APC BrdU Flow kit (BD Pharmingen, 552598) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and IL-17A) were simultaneously measured using the mouse Th1/Th2/Th17 cytokine cytometric bead array (CBA) kit (BD Biosciences). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: Apoptosis of the CTAC cells were determine by using FITC-Annexin V Apoptosis Detection kit (BD Pharmingen, San Diego, CA). The cells were treated with 5 μM of plumbagin for 18 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Single cells were sorted into the 96-well plates of the Precise WTA Kit with predispensed library chemistry using a BD FACSAriaII SORP (BD) and a gating strategy as displayed in Supplementary Figure 3 ...
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bioRxiv - Microbiology 2019Quote: Cytokines were measured in culture supernatants using Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD Bioscience, San Jose, CA, USA). The kit was used for the simultaneous detection of mouse monocyte chemoattractant protein-1 (MCP-1) ...
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bioRxiv - Immunology 2019Quote: Infection-inflicted early cell death was quantified by FACS analyses subsequent to staining of MEFs with FITC/Annexin-V and PI using apoptosis detection kit from BD Biosciences (# 556547) ...
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bioRxiv - Cancer Biology 2019Quote: ... the apoptosis of PANC-1 cells was determined by using the Annexin V-FITC/PI apoptosis detection kit (BD Biosciences). The cells were harvested with trypsin-EDTA (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... The concentrations of cytokines IL-2 and IFN-γ were quantified using the corresponding ELISA kits (BD, 555190 and 555142).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice with ice-cold PBS and FITC-annexin-PI was done as per manufacturer’s instruction using FITC Annexin V Apoptosis Detection Kit I (Cat. 556547, BD Biosciences). Flow cytometry was performed using a BD LSR II flow cytometer of the Flow Cytometry Core Facility at The Bloomberg School of Public Health ...
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bioRxiv - Microbiology 2020Quote: ... washed twice with cold PBS and then re-suspended in 1x binding buffer(Apoptosis detection Kit I # 556578, BD Pharmingen) at 1x 106 cells/ml ...
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bioRxiv - Microbiology 2020Quote: Mouse cytokines in the culture supernatants were measured with a BD OptEIA ELISA kit (BD Biosciences, Franklin Lakes, NJ, USA) as described previously (84) ...
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bioRxiv - Immunology 2021Quote: ... lung single cell suspensions were stained with tetramer prior to the surface and intracellular staining for 1 hour at 37°C.Intracellular cytokine staining was performed using the BD Cytofix/Cytoperm kit (BD Biosciences) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and fixed/permeabilized using a Cytofix/Cytoperm Buffer Kit (BD Biosciences) or a FoxP3 Transcription Factor Buffer Kit (eBioscience) ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Cells were washed with Perm/Wash and stained with 1µl/1mio cells anti-BrdU from the FITC BrdU Flow Kit (BD Biosciences) for 20min at RT in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with the fixable LIVE/DEADTM yellow stain according to the manufacturer’s protocol followed by fixation using the Fixation/Permeabilization kit (BD Biosciences). After fixation ...
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bioRxiv - Cell Biology 2021Quote: ... conjugated anti-c-kit and Peridinin Chlorophyll Protein Complex (PerCP) or allophycocyanin (APC) conjugated anti-CD45 antibodies were purchased from BD Biosciences Pharmingen ...
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bioRxiv - Cancer Biology 2022Quote: ... for 45 minutes and harvested for analysis of BrdU positive cells using the BrdU flow kit according to manufacturer’s protocol (BD Biosciences). Nuclei were stained with 7-AAD ...
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bioRxiv - Genomics 2022Quote: ... Obtained single-cell suspensions were subjected to a BD Rhapsody system with BD Rhapsody Targeted & Abseq Reagent kit (BD Biosciences) following the manufacture’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and live/dead fixable NIR dead cell stain kit reagent according to manufacturer’s directions and analysed on a 5-laser Fortessa X20 (BD Biosciences) as previously described (Zaunders et al. ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed by activating cells for 4-5h at 37°C with the Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) and subsequent fixing and staining per manufacturer’s instructions using the BD Fixation/Permeabilization Solution Kit (BD Biosciences). For intranuclear transcription factor staining ...
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bioRxiv - Immunology 2022Quote: ... Anti-mouse CD44-APC/Cy7 (clone IM7) and anti-mouse CD117-APC (c-Kit; clone 2B8) were obtained from BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... IL-10 and IL-17 were analyzed using the Cytometric Bead Array (CBA) Mouse Th1/Th2 Cytokine Kit (BD Biosciences) according to manufacturer’s instructions.
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... NK cell-specific surface markers were stained and cells were fixed and permeabilized using the Cytofix/Cytoperm Kit (BD Biosciences) before adding anti-IFN-γ and anti-TNF-α ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometric evaluation of intracellular cleaved caspase-3 was carried out using the FITC Active Caspase-3 Kit (BD Bioscience) and CD19 staining after 24 hour co-culture at a 1:4 E:T ratio.
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bioRxiv - Immunology 2022Quote: CD8+ T cells were enriched from naïve donor mice (CD43+/+ and CD43-/-) using BD IMagTM Mouse CD8 T Lymphocyte Enrichment Kit (BD Bioscience) and equal numbers of cells were transferred into TCRα-/- recipients.
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bioRxiv - Cell Biology 2019Quote: ... APC-conjugated anti-c-kit/CD117 (generated by the WEHI Antibody Facility) and PE-Cy7-conjugated anti-Sca1 (BD Pharmingen). Cells were stained with FluoroGold to assess viability and analysed on a BD LSRFortessa cell analyser.
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bioRxiv - Zoology 2020Quote: The apoptosis of erythrocyte was determined using PE annexin-V apoptosis detecting kit according to the manufacturer’s instruction (BD, USA). In brief ...
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bioRxiv - Immunology 2020Quote: ... antibodies were used for intra-cellular staining by using the Fixation/Permeabilization Solution Kit (BD Cytofix/Cytoperm™, BD Biosciences). For in vitro intracellular cytokine staining ...
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bioRxiv - Immunology 2020Quote: ... antibodies were used for intra-cellular staining by using the Fixation/Permeabilization Solution Kit (BD Cytofix/Cytoperm™, BD Biosciences). For in vitro intracellular cytokine staining ...
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bioRxiv - Immunology 2019Quote: ... IL-10 and TNF in the lung homogenates were measured using the Mouse Inflammation Cytokine Bead Array Kit (BD Biosciences) according the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... The number of CD11b receptors on CD45+CD11b+ TAMCs was quantified using the QuantiBRITE PE Fluorescence Quantitation Kit (BD Biosciences), according to the manufacturer’s recommended protocol.
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bioRxiv - Immunology 2019Quote: ... The cells were then incubated for 18 hours and IL-2 amounts in the supernatant were determined using a mouse IL-2 OptEIA ELISA kit from BD Biosciences as per manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Cytokine analysis was conducted on un-diluted plasma using a CBA Mouse Th1/Th2/Th17 Cytokine Kit to the maker’s instructions (BD biosciences).
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bioRxiv - Immunology 2020Quote: ... BV421 conjugated streptavidin was added for 20 minutes at 4 °C prior to fixation and permeabilisation (BD Fix perm kit). Anti IFN-γ-FITC (clone CC302 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed and permeabilized using BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit according to the manufacturer’s protocol (BD Biosciences). Cells were then incubated with anti-vaccinia virus primary antibody (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... both floating and adherent cells were harvested and analysed with the FITC Annexin V Apoptosis Detection Kit I (BD Bioscience), according to the manufacturer’s instructions ...
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Penicillium purpurogenum exerts antitumor effects and ameliorates inflammations in Erlich mice modelbioRxiv - Microbiology 2020Quote: ... using the BD ™ Cytometric Bead Array (CBA) cytokine kit Mouse Th1/Th2/Th17 (BD Biosciences, San Jose, CA, USA) following the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2021Quote: ... Apoptotic cells were detected by Accuri C6 fluorescence-activated cell sorting (FACS) using Annexin V-APC Apoptosis Detection Kit (BD #), according to the manufacturers protocol.
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bioRxiv - Cancer Biology 2020Quote: ... to analyze cell cycle distribution and we quantified apoptotic cells with PE-AnnexinV Apoptosis Detection Kit I (559763, BD Pharmingen).