Cystatin C ELISA Kit 1 Plate citations for Becton, Dickinson and Company

Quote: Cells from the cell line U3065 obtained from the Human Glioma Cell Culture (HGCC) resource [35] were suspended in stem cell medium and plated on 384 well plates (BD Falcon Optilux TC #353962) coated in laminin ...

Astrocyte- and neuron-derived extracellular vesicles from Alzheimer’s disease patients effect complement-mediated neurotoxicity

Carlos J Nogueras-Ortiz, et al., bioRxiv - Neuroscience 2020

Quote: ... and plated as dissociated cells (25 × 104 cells/well) on day −2 in a 6-well Matrigel-coated plate (BD Biosciences, San Jose, CA). On day −1 ...

Ex vivo and in vivo evidence that cigarette smoke-exposed T regulatory cells impair host immunity against Mycobacterium tuberculosis

Xiyuan Bai, et al., bioRxiv - Immunology 2022

Quote: ... The lungs and spleens were homogenized in saline and serial dilutions were plated on 7H11 agar plates supplemented with OADC (BD Biosciences, San Jose, CA). After 3-4 weeks of incubation at 37°C ...

Large-Scale Analysis of Cell Death Phenotypic Heterogeneity

Zintis Inde, et al., bioRxiv - Cancer Biology 2020

Quote: ... single cells were isolated and sorted into a 96 well plate using an Influx Special Order fluorescence activated cell sorter (BD Biosciences, San Jose, CA) at the Stanford Shared FACS Facility ...

Infection with CagA⁺ Helicobacter pylori induces epithelial to mesenchymal transition in human cholangiocytes

Prissadee Thanaphongdecha, et al., bioRxiv - Molecular Biology 2020

Quote: ... The base of the UC of the CIM plate was coated with 20% solution of a basement membrane matrix (BD Matrigel, BD Biosciences, San Jose, CA) in serum-free medium ...

Spontaneous suppressors against debilitating transmembrane mutants of CaMdr1 disclose novel interdomain communication via Signature motifs of the Major Facilitator Superfamily

Suman Sharma, et al., bioRxiv - Biochemistry 2022

Quote: ... Synthetic defined medium without uracil (SD-Ura−) plates were used that composed of 0.67% Yeast nitrogen base without amino acids (Difco, Becton, Dickinson and Company, MD, USA), 0.2% Ura− dropout mix (Sigma Genosys ...

Characterization of p38α signaling networks in cancer cells using quantitative proteomics and phosphoproteomics

Yuzhen Dan, et al., bioRxiv - Biochemistry 2022

Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.

Anaerobic degradation of syringic acid by an adapted strain of Rhodopseudomonas palustris

Julian Zachary Oshlag, et al., bioRxiv - Microbiology 2019

Quote: ... Photoheterotrophic growth on solid media was achieved by placing plates in a sealed canister containing a GasPakTM Ez Anaerobe Container System (BD Biosciences, Franklin Lakes, NJ), which was placed under constant illumination and rotated daily.

Inhalation: A means to explore and optimize nintedanib’s pharmacokinetic/pharmacodynamic relationship

Gali Epstein-Shochet, et al., bioRxiv - Pharmacology and Toxicology 2020

Quote: ... IPF-HLFs or nHLFs were cultured in 24-well plates previously coated with 10 mg/mL growth factor-reduced (GFR) Matrigel (BD Biosciences, San Jose, California). Following 48 h ...

Enhanced Production of Mesencephalic Dopaminergic Neurons from Lineage-Restricted Human Undifferentiated Stem Cells

Muyesier Maimaitili, et al., bioRxiv - Neuroscience 2021

Quote: ... FACS was then used to separate single RFP-positive cells in a 96-well plate using the 561 nm laser on a FACSAriaIII (BD Biosciences, San Jose, CA). Indels at the corresponding target sites in the clones were analyzed by genomic PCR ...

Mcc1229, an Stx2a-amplifying microcin, is produced in vivo and requires CirA for activity

Erin M. Nawrocki, et al., bioRxiv - Microbiology 2021

Quote: ... coli was suspended in 1 ml of spent supernatant to 0.05 OD600 and inoculated atop solid LB agar in a 6-well plate (BD Biosciences Inc., Franklin Lakes, NJ).

Pharmacologic Inhibition of DYRK1A Results in MYC Hyperactivation and ERK Hyperphosphorylation rendering KMT2A-R ALL Cells Sensitive to BCL2 Inhibition

Christian Hurtz, et al., bioRxiv - Cancer Biology 2022

Quote: One hundred thousand human ALL cells were seeded in a volume of 100 μL B cell medium/well (as described above) on Optilux 96-well plate (BD Biosciences, San Jose, CA). EHT1610 ...

Spontaneous myocarditis in mice predisposed to autoimmune disease: Including Vaccination-induced onset

Takuma Hayashi, Motoki Ichikawa, Ikuo Konishi, bioRxiv - Immunology 2022

Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...

Spatiotemporal regulation of the hepatocyte growth factor receptor MET activity by sorting nexins 1/2 in HCT116 colorectal cancer cells

Laiyen Garcia Delgado, et al., bioRxiv - Cancer Biology 2024

Quote: ... GFP-positive cells were selected and seeded individually in 96-well plates by flow cytometry (BD FACSAriaTM III, BD Biosciences, Franklin Lakes, NJ, USA). Double SNX1 and SNX2 KO cells were obtained using SNX2 KO cells ...

Hypoxia and TNF-alpha modulate extracellular vesicle release from human induced pluripotent stem cell-derived cardiomyocytes

Margarida Viola, et al., bioRxiv - Cell Biology 2024

Quote: ... 10 ng/ml TNF-α or exposed to hypoxia by placing the plate in a BD GasPak™ EZ Anaerobe Pouch System (BD Biosciences, 260683). After 48 hours ...

Stability of the Wheat Seed Mycobiome Across North Carolina’s Longitudinal Gradient

Lindsey E. Becker, et al., bioRxiv - Microbiology 2024

Quote: ... we rolled a subset of disinfested seeds on malt extract nutrient agar by shaking the plate for 30 s and subsequently removing seeds (BD Difco, Franklin Lakes, NJ). Plates were incubated at room temperature (25 ℃) ...

eIF2B Activator Rescues Neonatal Lethality of an eIF2Bα Sugar Phosphate Binding Mutation Associated with Vanishing White Matter Disease

James J. Lee, et al., bioRxiv - Neuroscience 2023

Quote: ... cells were single-sorted into 96-well plates containing complete media + ISRIB using a BD Biosciences FACSAria Fusion (BD Biosciences, San Jose, CA, USA). Clones were expanded and genomic DNA was sequenced to confirm the desired mutations ...

Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant SMO and GLI into Glioma Primary Cilia

Ping Shi, et al., bioRxiv - Cancer Biology 2022

Quote: ... individual GFP+ clones were sorted and expanded in 96-well plates each containing 250 μL of supplemented S7 medium without FBS using a BD FACS Aria II Cell Sorter (BD Biosciences, San Jose, CA). Cell debris were excluded from the analysis by forward- and side-scatter gating ...

Crystal ribcage: a platform for probing real-time lung function at cellular resolution in health and disease

Rohin Banerji, et al., bioRxiv - Bioengineering 2023

Quote: ... pneumoniae serotype 3 (Sp3) (ATCC; Manassas, VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences ...

CD28 Shapes T Cell Receptor Signaling by Regulating ZAP70 Activation and Lck Dynamics

Kumarkrishna Raychaudhuri, et al., bioRxiv - Immunology 2024

Quote: ... Lymphocytes were cultured in RPMI supplemented with 10% FBS and 50 μM beta-mercaptoethanol (RPMI media) Lymphoblasts were generated by activation with plate bound murine anti-CD3ε antibody (BD Biosciences, Clone: 145-2C11) in the presence of 100 U/ml of human IL-2 and soluble anti-CD28 antibody (BD Biosciences ...

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