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Citations for Becton, Dickinson and Company :
2201 - 2250 of 3433 citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 5 Strip Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences). Basal IFN-γ production by PBMCs was included as background control in the absence of stimulation and co-stimulation ...
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bioRxiv - Immunology 2019Quote: ... for 5 min at room temperature before acquisition and sorting of the cells using a BD FACSARIA III (BD BioSciences, USA). Single antibody staining was prepared in parallel to assess fluorescence spillover ...
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bioRxiv - Cancer Biology 2019Quote: ... Transfected cells were selected with 5 μg/mL puromycin and RFP+ cells were collected using fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were plated at a density of 1.5×106 cells/well on 18mm coverslips (Neuvitro 18-GG-PDL) precoated with high concentration growth factor-reduced Matrigel (BDBiosciences, 354263) and cultured at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked for 5 minutes at room temperature using anti-mouse CD16/32 Fc block (1/50) (BD Biosciences). Cells were then incubated with the staining (Ly6g-APCCy7 (1A8) ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μL of the cell suspension (more than 1×105 cells) was transferred to a 5 mL FACS tube (BD Biosciences) and stained in duplicate with 10 μL of FITC Annexin V conjugate and 10 μL of propidium iodide (PI ...
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bioRxiv - Microbiology 2020Quote: ... A minimum of 5 000 – 10 000 cells was analyzed per sample for YFP expression on a LSRII flow cytometer (BD Biosciences). Data was analyzed using Flowing Software (Version 2.5).
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bioRxiv - Immunology 2022Quote: ... and tissue-resident alveolar macrophages (TRAMs) were flow-sorted using a BD FACSaria III 5-laser cell sorter and 100 μm nozzle (BD Biosciences). Of note ...
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bioRxiv - Microbiology 2021Quote: ... 0.5–1 million cells were stained with one of the following primary antibodies: mouse anti-PSGL1 antibody (KPL-1) (BD Pharmingen), mouse anti-CD43 antibody (1G10 ...
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Microbiology 2020Quote: ... The resulting bacterial suspension was serially diluted and plated on BD Group A Selective Strep Agar with 5% Sheep Blood (BD Diagnostics) or THY agar plates with appropriate antibiotic selection ...
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bioRxiv - Immunology 2021Quote: ... The bacterial pellet was resuspended in 1X PBS + 0.25% BSA + 5% NGS and stained with Fc Block (BD Biosciences, clone 2.4G2), followed by SYTO BC (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... CD8+EGFP-Tconv and CD8+EGFP+ Treg cells in blood and spleen as well as the expression levels of Stat-5 phosphorylation (clone pY694, BD Biosciences) and CD25 (clone OX39 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed two times with flow buffer (50 nM EDTA, 5% FBS in PBS) before acquiring them on the FACSymphony A5 (BD Biosciences). The gating strategy is outlined in Suppl Fig 1C.
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bioRxiv - Immunology 2021Quote: ... All samples were acquired with a FACS Canto II or 5 laser Fortessa with BD FACS Diva software and analysed with FlowJo software (versions 9 and 10; BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Immunology 2022Quote: ... were re-stimulated in vitro for 5h at 37°C 5% CO2 in cRPMI supplemented with GolgiStop (1/250; BD bioscience), GolgiPlug (1/500 ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Genomics 2022Quote: ... and then dounce homogenized (10 loose strokes and 5 tight strokes) and filtered through 35-40μm cell strainers (BD Biosciences, 352235). Nuclei were pelleted (600g ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Microbiology 2022Quote: ... 50 μL of DEs were diluted into 500 μL of FACS diluent buffer (PBS + 1% Tween-20) in a 5 mL 12 × 75 mm round bottom FACS tube (BD Biosciences). After standard autocalibration on the Sony SH800 ...
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bioRxiv - Bioengineering 2022Quote: ... The specificity of CD8+ T cells towards the peptide was previously assessed by co-culture of 5·104 T cells with pulsed tumor cell lines in the presence of Golgi Plug (BD Biosciences) in 96-well plate at 1:1 E:T cell ratio.
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were stained with Alexa Fluor 647-conjugated rat anti-CD49f antibody (5% v/v in a 100 µL reaction; BD Biosciences) for 25 minutes at 37°C on an orbital shaker at 200 rcf and resuspended in DPBS containing 0.5% bovine serum albumin and 50 units/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-MPO-FITC (clone M1/70, catalog no. 553310; BD Biosciences), Fc Block ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% BSA in Dulbecco’s PBS) analyzed using the Guava® easyCyte 5 HPL benchtop flow cytometer (MerckMillipore) or BD LSRFortessaTM cell analyzer (BD Biosciences). Data analysis was performed using FlowJo version 10 (FlowJo ...
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
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bioRxiv - Microbiology 2023Quote: Cells from BALF were centrifuged at 300 x g for 5 minutes and incubated in 500μl Pharm Lyse Buffer (BD Biosciences 555899) for 3 minutes at room temperature to lyse red blood cells ...
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bioRxiv - Cancer Biology 2023Quote: ... digested lung cells were resuspended in 400 μl of buffer with 5 μl of anti-CD31-FITC (BD Biosciences, CA, USA), 5 μl of anti-CD45-APC (BD Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: ... Gates were determined using the isotype controls mouse anti-IgG2b κ (APC-conjugated, Clone 27-35, 1:5, BD Biosciences, US) and mouse anti-IgG2a κ (PE-Cy7-conjugated ...
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bioRxiv - Microbiology 2023Quote: ... The seawater samples were then diluted with culture media to a concentration of 5 cells mL-1 and dispensed (1 mL per well) into 48-well microplates (BD Falcon). The plates were incubated at 20 °C for 1 month in dark or under LED light (Philips ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed in 3ml FACS buffer before being fixed in 1% paraformaldehyde and analyzed within 5 days using a Fortessa Flow Cytometer and BD FACSDiva Software (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... Phycoerythrin (PE) or peridinin chlorophyll protein -cyanine 5.5 (PerCP-Cy5.5)-conjugated CD3 (BD Biosciences Cat#5 61808, BD Biosciences Cat# 55163); Pacific Blue-conjugated (PB) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 x 106 dissociated organoid cells in 100 µl hepatocyte culture medium and 5% Matrigel were injected subcutaneously into the flank using a 1 ml syringe with 25G needle (BD 305122). For human grafts ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Neuroscience 2023Quote: ... and non-neuronal (NeuN-) nuclei were sorted into tubes pre-coated with 5%BSA using a BD-FACSAria flow cytometer (BD Biosciences) equipped with a 70μm nozzle (Figure S6) ...
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bioRxiv - Immunology 2023Quote: ... The cells were cultured at 37°C and 5% CO2 for 72 hours and then the dye dilution was analyzed using a FACSVerse flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).
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bioRxiv - Cell Biology 2023Quote: ... Then cells were washed twice with HBSS-5%FBS and once with PBS and analysed by flow cytometry (BD FACScalibur, BD Bioscience).
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bioRxiv - Immunology 2023Quote: ... P14 T cells (Figure 5) were FACS-sorted from the spleen or LNs of chronically infected mice using a FACSAria III cell sorter (BD Biosciences). To multiplex different samples for scRNA sequencing ...
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bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
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bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
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bioRxiv - Bioengineering 2023Quote: ... and then cells were re-suspended in 150 μL of PBS with 5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression.
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bioRxiv - Cell Biology 2022Quote: ... Samples were stimulated with the indicated agonists and labelled with a PS detecting antibody (CyTM 5 Annexin V, #559934, BD Biosciences) for 15 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used: Anti-CD166 mouse monoclonal antibody conjugated with PE (phycoerythrin) at 5 uL (BD Biosciences #559263), anti-LRIG1 sheep polyclonal unconjugated antibody at 2.5 ug (Invitrogen #PA5-47928) ...
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bioRxiv - Immunology 2023Quote: ... Cells were acquired on a Cytek Aurora spectral flow cytometer using Cytek SpectroFlo software (Cytek; the HD BM samples) or a LSR Fortessa X20 (special order research product with 5 lasers; BD Biosciences) and analyzed using FlowJo software (v10.8.1 ...
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bioRxiv - Biophysics 2022Quote: ... #131-8; #130-6; #130-5; #130-3 and 2 μm lot MM2307#156; #159.1; #159.2; #122.3, BD Biosciences, San Jose, CA). For calibration of the fluorescence axis in the PE channel ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were discarded and cells resuspended in FACS buffer and tetramer-binding B cells were individually sorted using a 5-laser (355nm, 405nm, 488nm, 561nm, 640nm) FACS Aria (BD Biosciences) into empty 96-well low profile PCR plates (Labcon) ...