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Citations for Becton, Dickinson and Company :
2101 - 2150 of 7309 citations for 6 Methyl benzo 1 3 dioxole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The bacterial pellet was resuspended in 1X PBS + 0.25% BSA + 5% NGS and stained with Fc Block (BD Biosciences, clone 2.4G2), followed by SYTO BC (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... CD8+EGFP-Tconv and CD8+EGFP+ Treg cells in blood and spleen as well as the expression levels of Stat-5 phosphorylation (clone pY694, BD Biosciences) and CD25 (clone OX39 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed two times with flow buffer (50 nM EDTA, 5% FBS in PBS) before acquiring them on the FACSymphony A5 (BD Biosciences). The gating strategy is outlined in Suppl Fig 1C.
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bioRxiv - Immunology 2021Quote: ... All samples were acquired with a FACS Canto II or 5 laser Fortessa with BD FACS Diva software and analysed with FlowJo software (versions 9 and 10; BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Genomics 2022Quote: ... and then dounce homogenized (10 loose strokes and 5 tight strokes) and filtered through 35-40μm cell strainers (BD Biosciences, 352235). Nuclei were pelleted (600g ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Bioengineering 2022Quote: ... The specificity of CD8+ T cells towards the peptide was previously assessed by co-culture of 5·104 T cells with pulsed tumor cell lines in the presence of Golgi Plug (BD Biosciences) in 96-well plate at 1:1 E:T cell ratio.
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bioRxiv - Cell Biology 2023Quote: ... digested lung cells were resuspended in 400 μl of buffer with 5 μl of anti-CD31-FITC (BD Biosciences, CA, USA), 5 μl of anti-CD45-APC (BD Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The activated Ramos cells were incubated overnight (18 h) in 5% CO2 at 37°C followed by staining for flow cytometry (BD LSR2) and analysis (FlowJo and Graphpad Prism).
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bioRxiv - Microbiology 2024Quote: ... The antibodies employed for ELISA were biotin-conjugated rat anti-mouse IgG2a (clone R19-5, BD Biosciences, Denmark cat no. 550332) and anti-mouse IgG conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... cell suspension was incubated with Fc block antibodies for 5 minutes at 4 °C and then labeled with anti-mouse Thy1.2 magnetic particles (BD IMag™) for 30 minutes at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% BSA in Dulbecco’s PBS) analyzed using the Guava® easyCyte 5 HPL benchtop flow cytometer (MerckMillipore) or BD LSRFortessaTM cell analyzer (BD Biosciences). Data analysis was performed using FlowJo version 10 (FlowJo ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were stained with Alexa Fluor 647-conjugated rat anti-CD49f antibody (5% v/v in a 100 µL reaction; BD Biosciences) for 25 minutes at 37°C on an orbital shaker at 200 rcf and resuspended in DPBS containing 0.5% bovine serum albumin and 50 units/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-MPO-FITC (clone M1/70, catalog no. 553310; BD Biosciences), Fc Block ...
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bioRxiv - Cancer Biology 2023Quote: ... or ASC52telo cells (ATCC SCRC-4000) were suspended in 5%FBS/PBS solution and the following antibodies were added: APC-CD14 (BD 561708), APC-CD19 (BD 561742) ...
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bioRxiv - Cancer Biology 2024Quote: ... at 9:00 am 5 mL of fasted blood from the antecubital vein was collected into an EDTA Vacutainer tube (BD, 366643), then centrifuged at 800 x g for 10 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: Cells from BALF were centrifuged at 300 x g for 5 minutes and incubated in 500μl Pharm Lyse Buffer (BD Biosciences 555899) for 3 minutes at room temperature to lyse red blood cells ...
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bioRxiv - Cancer Biology 2023Quote: ... digested lung cells were resuspended in 400 μl of buffer with 5 μl of anti-CD31-FITC (BD Biosciences, CA, USA), 5 μl of anti-CD45-APC (BD Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... and non-neuronal (NeuN-) nuclei were sorted into tubes pre-coated with 5%BSA using a BD-FACSAria flow cytometer (BD Biosciences) equipped with a 70μm nozzle (Figure S6) ...
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bioRxiv - Immunology 2023Quote: ... P14 T cells (Figure 5) were FACS-sorted from the spleen or LNs of chronically infected mice using a FACSAria III cell sorter (BD Biosciences). To multiplex different samples for scRNA sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... Phycoerythrin (PE) or peridinin chlorophyll protein -cyanine 5.5 (PerCP-Cy5.5)-conjugated CD3 (BD Biosciences Cat#5 61808, BD Biosciences Cat# 55163); Pacific Blue-conjugated (PB) ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were washed twice with HBSS-5%FBS and once with PBS and analysed by flow cytometry (BD FACScalibur, BD Bioscience).
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bioRxiv - Immunology 2023Quote: ... The cells were cultured at 37°C and 5% CO2 for 72 hours and then the dye dilution was analyzed using a FACSVerse flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).
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bioRxiv - Bioengineering 2023Quote: ... and then cells were re-suspended in 150 μL of PBS with 5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression.
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bioRxiv - Cell Biology 2022Quote: ... Samples were stimulated with the indicated agonists and labelled with a PS detecting antibody (CyTM 5 Annexin V, #559934, BD Biosciences) for 15 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used: Anti-CD166 mouse monoclonal antibody conjugated with PE (phycoerythrin) at 5 uL (BD Biosciences #559263), anti-LRIG1 sheep polyclonal unconjugated antibody at 2.5 ug (Invitrogen #PA5-47928) ...
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bioRxiv - Immunology 2023Quote: ... Cells were acquired on a Cytek Aurora spectral flow cytometer using Cytek SpectroFlo software (Cytek; the HD BM samples) or a LSR Fortessa X20 (special order research product with 5 lasers; BD Biosciences) and analyzed using FlowJo software (v10.8.1 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were discarded and cells resuspended in FACS buffer and tetramer-binding B cells were individually sorted using a 5-laser (355nm, 405nm, 488nm, 561nm, 640nm) FACS Aria (BD Biosciences) into empty 96-well low profile PCR plates (Labcon) ...
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bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
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bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
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bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... while 100 μg was subjected to chromatin immunoprecipitation (ChIP) using 5 μg of mouse anti-ASCL1 (Mash1) antibody (BD Biosciences, 556604) or 5 μg rabbit ant-OLIG2 (Olig2 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting colonies were inoculated into 5 mL of marine broth medium (BD Difco Marine Broth 2216; Becton, Dickinson and Company), which was shaken at 300 rpm and 25°C until bacterial growth was confirmed ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... prior to stimulation for 5 h with anti-CD3 and anti-CD28 without IL-2 in the presence of brefeldin A and monensin (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Bioengineering 2023Quote: ... VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences) in a 37°C humidified 5% CO2 environment for 10-11 hours and diluted in 0.9% sodium chloride to the desired optical density ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x with FACs buffer at 1000xg for 5 minutes at room temperature and analyzed on a Canto II (BD Biosciences) at the Flow Cytometry Shared Resource at the University of Arizona or on a BD Fortessa ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9-BFP+ cells were sorted for top 5% of the brightest GFP signal simultaneously on two BD FACSAria II Cell Sorters (BD Biosciences) equipped with blue 488 nm and violet 405 nm lasers ...
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bioRxiv - Immunology 2024Quote: ... Cells were spun down at 300 g for 5 min and supernatant was collected for analysis by mouse inflammatory cytokine bead array (CBA, BD Biosciences). Cells were resuspended and stained for CD4 and analyzed for proliferation based on CFSE decrease (Fig SI 3).
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bioRxiv - Developmental Biology 2024Quote: ... 1 – 2 mL of thin albumin was aspirated out with a 5 mL syringe (Nipro) and a 18G sterile needle (BD PrecisionGlide) from the blunt end of the egg to create a space between the embryo and the shell ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in 1X PBS with 1X protease/phosphatase inhibitor cocktail in and filtered through a 5 ml cell-strainer capped FACS tube (BD Falcon). Cell sorting was performed on BD Biosciences FACS Aria Fusion ...
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bioRxiv - Immunology 2024Quote: ... Data was acquired using a Cytek Aurora 5-laser flow cytometer or ImageStream cytometer (CytekBio) and subsequently analyzed FlowJo V10 (BD Biosciences).
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bioRxiv - Cell Biology 2020Quote: Antibody dilutions: mouse anti-p62 (1:100, BD Bioscience – 1:200 for STED microscopy); rabbit anti-p62 (1:500 ...