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Citations for Becton, Dickinson and Company :
2101 - 2150 of 7431 citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... washed with cold PBS by centrifugation (5 min, 550g at 4°C) and analysed with a FACS BD AccuriTM C6 (BD Biosciences) instrument ...
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bioRxiv - Microbiology 2020Quote: ... cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Pacific Blue; BD Biosciences), anti-CD4 (L200 ...
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bioRxiv - Cell Biology 2020Quote: ... half of the medium (50 μl) per well was replaced with fresh differentiation medium containing 4% (v/v) Matrigel (BD Bioscience). On day 3 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were incubated for 30 min in the dark at 4°C after adding relevant antibodies (BD biosciences, Franklin Lakes, NJ). A FACSCalibur system (BD biosciences ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
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bioRxiv - Cell Biology 2022Quote: Cells harvested at different time points of reprogramming were stained in FACS buffer for 30 min at 4°C and washed with FACS buffer prior to acquisition with LSR Fortessa (BD Biosciences) cytometer ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... Binding of mAbs to CTLA-4 on the cell surface was determined using LSR II (BD Biosciences, San Jose, CA, USA) or CytoFLEX LX (Beckman Coulter ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences). For subsequent limiting dilution cloning ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4℃ after 1h blocking in 5% skimmed milk (Becton, Dickinson and Company, USA) at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were fixed in 4% paraformaldehyde for 30 minutes and stained with PE-conjugated or purified CD45 antibody (eBioscience, 30-F11 or BD Biosciences) to ascertain the generation of haematopoietic progeny ...
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bioRxiv - Microbiology 2019Quote: ... transduced cells were sorted for single positive mCherry signal or double positive mCherry/eGFP signal at 4°C using FACS Aria III and the corresponding FACS Diva software (BD Bioscience). Cells were sorted from HBSS supplemented with 10 µM Y-27632 ...
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bioRxiv - Genomics 2021Quote: The titer of the lentivirus containing either the genome-wide library or distal sub-library of gRNAs was determined by transducing 5×105 cells with varying dilutions of lentivirus and measuring the percentage of GFP-positive cells 4 days later using the Accuri C6 flow cytometer (BD Biosciences).
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bioRxiv - Genomics 2021Quote: ... tissues were blocked (3% bovine serum albumin in 5% normal goat sera and 0.1% Triton) and incubated overnight at 4°C with primary antibodies: CD31 (Neomarkers, RB-10333-P0) or CDH1 (BD Biosciences, 6315829). Slides were washed and incubated with Cy3-goat-anti-mouse or anti-rabbit-conjugated secondary antibodies (Jackson Immunoresearch Laboratories ...
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bioRxiv - Genetics 2019Quote: ... The DAPI-stained cells were resuspended in FACS buffer and then analyzed by Fortessa 4-15 HTS or Fortessa X20 5-18 flow cytometry analyzers (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... the killing capacity of NK cells was tested when they coculture with senescent / young or modulated for ST8SIA1 expression MRC5.NK cells are added to the culture for 4 h in presence of monensin and brefeldin (BD Biosciences) at the effector/target ratio of 1:1 ...
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bioRxiv - Immunology 2021Quote: Cultured T cells were stimulated with the cognate SARS-CoV-2 OPPs (ORF1ab-C, ORF3a, N, S, nsp12) and incubated for 4 hours in the presence of Golgiplug (BD Biosciences). Following stimulation ...
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bioRxiv - Bioengineering 2020Quote: Cell surface staining was performed at 4 °C for 30 min and was analysed using a FACSCalibur flow cytometer (BD Biosciences) and CellQuest Software (BD Biosciences) ...
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bioRxiv - Bioengineering 2020Quote: Cell-surface staining was performed for 45 min at 4 °C and was analyzed using a FACSCalibur flow cytometer (BD Biosciences). A minimum of 1 × 104 events per sample were examined.
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Biophysics 2020Quote: ... Cells were washed once and fluorescence intensities were quantitated using a 4-laser Fortessa flow cytometer (BD Bioscience, San Jose, CA). To correct the fluorescence intensity difference of the two antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were again pelleted at 500xg for 5 min at 4°C then resuspended in Cytofix/Cytoperm reagent (BD Biosciences) and incubated on ice for 30 min Following fixation/permeabilization ...
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bioRxiv - Microbiology 2021Quote: ... fixed with 4% formaldehyde for 15 min and washed and resuspended in FACS buffer before analyzing by flow cytometry using FACSCelesta (BD Biosciences) or Cytek Aurora spectral analyzer (Cytek Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... Selection of the positive cells was performed using 4 µg/ml puromycin and viable cells were expanded for flow cytometry cell sorting using a FACSMelody (BD Biosciences) equipped with a 561 nm excitation laser and 613/18 filter ...
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bioRxiv - Synthetic Biology 2021Quote: Streptomyces coelicolor M1146 and derivative strains were routinely maintained on Soya-Mannitol Flour (SFM) agar supplemented with 10 mM MgCl2 and International Streptomyces Project medium #4 (ISP4) (BD Difco) at 30°C as described previously [34] ...
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bioRxiv - Bioengineering 2022Quote: ... Genes encoding the proteins were cloned into pET29b between the restriction sites NdeI and XhoI and expressed for 4 h in Terrific Broth (BD Difco) using Lemo21 (NEB ...
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bioRxiv - Physiology 2022Quote: ... Cell suspensions were washed and re-suspended in PBS at 4°C and analysed using a BD 5L LSR Fortessa and BD FACSDiva software (BD Biosciences). To exclude dead cells ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... for 20 minutes and then with a blood antibody cocktail for 30 minutes in the dark at 4 degrees: anti-CD45 BB515 (BD Horizon), anti-CD14 PerCP-Cyanine5.5 (eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... 1 x 106 popliteal LN cells/ml were stimulated with 10 μg/ml of PHA for 4 days and cytokines levels were measured by FACS using Cytometric Bead Assay (CBA; BD bioscience).
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bioRxiv - Neuroscience 2021Quote: ... and their Fc receptors were blocked for 10 min at 4 °C with anti-CD16/CD32 antibodies (10 μg/mL: BD Biosciences). After blocking ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with fluorochrome-conjugated antibodies for 30min at 4°C in the dark in brilliant stain buffer (BD Biosciences). Prior to fixation and permeabilization for intracellular staining with Cytofix/Cytoperm (BD Bioscience) ...
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bioRxiv - Immunology 2020Quote: ... The cells were incubated for one hour at 4°C with a cocktail of fluorochrome-labelled antibodies consisting of anti-CD3-BV510 (cat. no: 563024, BD Biosciences), anti-B220-APC-Cy7 (cat ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested 4 days post-transfection and subjected to fluorescence-activated cell sorting (FACS) for RFP with a FACS Aria III (BD Biosciences). Genomic DNA from sorted RFP-positive cells was extracted using DNeasy Blood & Tissue Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...
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bioRxiv - Microbiology 2021Quote: Spleen and liver cells were incubated (20 min, 4°C) with anti-Fc-gamma blocking antibody (clone 2.4G2) (BD Biosciences, Belgium) and were subsequently stained with fluorescent conjugated antibodies (30 min ...
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bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A and MCF-10A MYC-ER cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). Cells were lysed in Laemmli buffer (50 mM Tris-HCl pH 6.2 ...
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bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). 2D-grown HEK293T ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF-10A and MCF-10A MYC-ER cells were seeded at a density of 10,000 cells per well in triplicate on a 4-well glass chamber slide coated with Matrigel Growth Factor Reduced (BD Biosciences) as described (Debnath et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated during 30 minutes at 4°C with the following primary antibodies: CD11b (clone M1/70, AF647, #557686, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated with the following primary antibodies during 30 minutes at 4°C: CD11b (clone M1/70, APC-Cy7, #557657, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2022Quote: ... whereas 100 µl PBS+/+ containing 20 ng/ml recombinant IL-1β supplemented with 100 µg/ml PTX3 and/or 100 µg/ml mouse anti-human CD62P (clone AK-4, BD Bioscience) was added to the apical side (upper chamber) ...
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bioRxiv - Immunology 2022Quote: ... single cell suspensions were fixed with CytoFix/CytoPerm for 30 minutes at 4°C followed by permeabilization with BD CytoPerm Plus Buffer (BD Biosciences) for 15 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stored in DPBS with 2% FBS overnight at 4°C before data acquisition on a LSR6Fortessa analyser (BD Biosciences). Compensation was carried out using single stain control UltraComp eBeads (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Colonic LP samples (2 million cell/staining) were stained at 4°C for 20 min with the following antibodies: Ly6C-FITC (AL-21, BD Pharmingen), F4/80-PE (BM8 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C and secondary antibodies for 30 min in RT in Perm/Wash buffer (554714, BD Biosciences) containing 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 4% heat-inactivated FCS containing FACS buffer and data were acquired by a Symphony Flow Cytometer (BD Biosciences). Flow cytometry and t-SNE analyses were performed with FlowJo software (FlowJo10.5.3) ...
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bioRxiv - Immunology 2020Quote: ... IL-4 and IL-10 were quantified using the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine kit (BD Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained overnight at 4°C in 1X perm/wash buffer with FITC mouse Ki67 set (BD Pharmingen #556026). Cells were then washed with 1X perm wash buffer and re-suspended in PBS supplemented with 1ug/ml 4′,6-diamidino-2-phenylindole
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bioRxiv - Genomics 2020Quote: ... Cells were then incubated with 5ul human Fc Block with 95ul 2% FBS in PBS at 4°C for 15 mins before staining with SCMK and AbSeq antibodies (BD Biosciences) at 4°C for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells with normal mCherry fluorescence (mid 70-80% fluorescence) and top or bottom ~7% eGFP fluorescence (~4 million cells/population) were sorted using a BD FACSAria III (BD Biosciences) and collected into 15 ml polypropylene tubes (Cellstar ...