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Citations for Becton, Dickinson and Company :
2051 - 2100 of 3459 citations for DHEA S ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The worms were immobilized with levamisole (final concentration 200 µM) and cut open using with a pair of 30G 5/8” needles (PrecisionGlide, BD) in a scissors motion to extract the tissues ...
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bioRxiv - Immunology 2024Quote: ... Tregs were then sorted on a BD FACS Aria Fusion based on viability dyenegative CD4+ (BV605, clone RM4-5, BD) GFP-FOXP3+ ...
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bioRxiv - Immunology 2024Quote: ... live EGFP+ or EGFPneg Tcf7+/EGFP P14 CD90.1+ TE (Zombie Aquaneg EGFP+ or EGFPneg CD8a+ CD90.1+) from helped or helpless spleens were sorted on a 5-laser FACS Aria II sorter (BD Biosciences). Small aliquots of sorted samples were checked for purity (on average >98%) ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
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bioRxiv - Immunology 2024Quote: ... Mouse peripheral blood was collected via submandibular bleed using Goldenrod 5 mm animal lancets (MEDIPoint) into K2EDTA microtainer tubes (BD). For all specimen types ...
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bioRxiv - Microbiology 2024Quote: ... A single isolated colony was then inoculated in 5 ml of BHI broth (BD, cat. no. 237500, Franklin Lakes, NJ) in a 15 ml conical tube (VWR ...
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bioRxiv - Developmental Biology 2023Quote: ... collected by centrifugation at 300 g for 5 min and passed through a 35-µm cell strainer filter (BD Biosciences). To assess viability ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pelleted for 5 min at 650 g and resuspended in PBS to be immediately analyzed using a Becton Dickinson (BD) LSRFortessa ...
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bioRxiv - Microbiology 2023Quote: ... We then sheared the flagella by repeated passage through two 5-ml syringes connected by 22-inch-long tubing (ID 0.023”, BD INTRAMEDIC, BD Medical), about 30 times ...
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bioRxiv - Cell Biology 2023Quote: ... and stained in 100 µL of FACS buffer (HBSS with 0.1% BSA w/v, 5 mM EDTA) containing mouse antibodies anti-CD45 (BD Sciences), anti-CD11b (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... Experimental starters were prepared from frozen master stocks plated on 5% Columbia blood agar plates (Biomerieux ref no.43041) and grown overnight at 37°C with 5% CO2 prior to outgrowth in Todd-Hewitt (BD) broth supplemented with 50mM HEPES (TH+H ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then washed using FACS Wash Buffer containing 1x D-PBS + 0.5% FBS + 2mM EDTA and permeabilized at room temperature for 20 minutes using 100µL of Perm/Wash I (BD # 557885), diluted 1:10 with 1x D-PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was then centrifuged at 300 × g for 5 min at 4 °C and cells resuspended in 100 µL of stain buffer (BD Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... 4×106 cells per sample were incubated in blocking solution containing 5% fetal bovine serum and 2% FcBlock (BD Biosciences) in PBS ...
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bioRxiv - Genetics 2023Quote: ... and the pellet was resuspended in 300 µL PBS with 5% FBS for flow cytometry using a CantoII (speed high; BD).
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bioRxiv - Immunology 2023Quote: ... were co-cultivated with 5×105 IE1-CTLL cells for 5h at 37°C in the presence of brefeldin A (BD GolgiPlug ...
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bioRxiv - Immunology 2023Quote: ... Sorting was conducted at room temperature and sorted cells were collected in 5 ml round-bottom polystyrene tubes (BD Biosciences) containing 500 μl of complete medium (RPMI 1640 supplemented with 10% FBS and 1% penicillin/streptomycin) ...
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bioRxiv - Microbiology 2023Quote: ... 50 μL of the stain cocktail (1x Brillant Stain Buffer [BD Bioscience] and either 5 μL per sample of Alexa Flour 647 Anti-Total Stat1 [N-Terminus] [BD Biosciences] and 5 μL per sample of PE anti-STAT1 Phospho [Tyr701] [BioLegend] ...
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bioRxiv - Genetics 2022Quote: The sorted cells were collected in 36 (3 samples × 12 bins) 5-mL polystyrene round-bottom centrifuge tubes (BD Falcon), each of which contained 500 μL of PBS ...
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bioRxiv - Immunology 2023Quote: ... detection antibody (IFNγ, BD Biosciences #51-1818KA; IL-17A, Invitrogen #88-7371-88; and IL-5, BD Biosciences #51-1806KZ) was diluted in ELISpot diluent (eBiosciences ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 3 hours at 37°C with 5% CO2 in R10 buffer supplemented with 0.33 μl/ml GolgiStop (BD Biosciences) and 15 μg/ml DNase I ...
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bioRxiv - Bioengineering 2023Quote: ... A tissue suspension was constituted by adding 5 mL of Tumor & Tissue Dissociation Regent (TTDR, BD Bioscience, San Jose, CA), and then incubating the mixture at 37 °C for 30 min with frequent agitation ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes containing the transferred proteins were rinsed and blocked in 0.1% Tween-20 in Tris-buffered saline (TBST) containing 5% nonfat milk (BD Biosciences) at RT for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... All dissociated cells were blocked with Fc receptors staining buffer (1% anti-mouse CD16/CD32, 2.4 G2, 2% FBS, 5% NRS, and 2% NMS in HBSS; BD Bioscience) and then stained with a standard panel of antibodies ...
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bioRxiv - Genomics 2023Quote: The following antibodies were used for Immunoblot analysis (Extended Data Table 5): Purified Mouse Anti-PTEN (BD Bioscience, Cat# 559600), Phospho-Akt (Ser473 ...
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bioRxiv - Physiology 2024Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 2.5 % donkey serum in PBST containing 0.2% Triton X-100) and incubated with α-EPS8 antibody produced in mouse (BD Transduction Laboratories™ ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice (900 x g for 5 minutes at room temperature) and then 50µL of surface antibody cocktail diluted in Brilliant Violet buffer (BD Biosciences) added and incubated 20 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... stained with 2.5 μM cisplatin for 5 min at 25°C and fixed using transcription factor buffer set (BD Biosciences), followed by intracellular staining for 60 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... and stored in FB 4C overnight before analysis on a 3 laser Aurora spectral flow cytometer (Cytek, figure 1D, Supplementary Figure 2) or X20 Special Order 5-lasers Fortessa (BD) flow cytometer (other figures) ...
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bioRxiv - Microbiology 2024Quote: ... The overnight cultures of Pseudomonas aeruginosa ATCC27853 and EXT111762 were adjusted to McFarland Standard 1 and further diluted to 5 × 105 cells mL−1 in cation adjusted Mueller Hinton II broth (BD). A volume of 5 mL was distributed to each cultivation flask and supplemented with either 0.5x ...
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bioRxiv - Immunology 2024Quote: ... according to manufacturer’s instructions to exclude dead cells and then labelled in FACS buffer with the following antibodies: V500 Rat anti-Mouse CD4 (RM4-5, BD), eF450 CD5 Monoclonal Antibody (53-7.3 ...
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bioRxiv - Cancer Biology 2021Quote: Cells from the cell line U3065 obtained from the Human Glioma Cell Culture (HGCC) resource [35] were suspended in stem cell medium and plated on 384 well plates (BD Falcon Optilux TC #353962) coated in laminin ...
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bioRxiv - Neuroscience 2020Quote: ... and plated as dissociated cells (25 × 104 cells/well) on day −2 in a 6-well Matrigel-coated plate (BD Biosciences, San Jose, CA). On day −1 ...
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bioRxiv - Immunology 2022Quote: ... The lungs and spleens were homogenized in saline and serial dilutions were plated on 7H11 agar plates supplemented with OADC (BD Biosciences, San Jose, CA). After 3-4 weeks of incubation at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... single cells were isolated and sorted into a 96 well plate using an Influx Special Order fluorescence activated cell sorter (BD Biosciences, San Jose, CA) at the Stanford Shared FACS Facility ...
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bioRxiv - Molecular Biology 2020Quote: ... The base of the UC of the CIM plate was coated with 20% solution of a basement membrane matrix (BD Matrigel, BD Biosciences, San Jose, CA) in serum-free medium ...
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bioRxiv - Biochemistry 2022Quote: ... Synthetic defined medium without uracil (SD-Ura−) plates were used that composed of 0.67% Yeast nitrogen base without amino acids (Difco, Becton, Dickinson and Company, MD, USA), 0.2% Ura− dropout mix (Sigma Genosys ...
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bioRxiv - Neuroscience 2020Quote: ... The transfer of the retinal suspension onto panning plates bound with monoclonal mouse anti-SSEA-1 (CD15)(MC480 clone) (#BD560079; BD Pharmingen, San Jose, CA) and monoclonal mouse anti-HNK-1/N-CAM (CD57)(VC1.1 clone ...
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bioRxiv - Biochemistry 2022Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.
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bioRxiv - Microbiology 2019Quote: ... Photoheterotrophic growth on solid media was achieved by placing plates in a sealed canister containing a GasPakTM Ez Anaerobe Container System (BD Biosciences, Franklin Lakes, NJ), which was placed under constant illumination and rotated daily.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... IPF-HLFs or nHLFs were cultured in 24-well plates previously coated with 10 mg/mL growth factor-reduced (GFR) Matrigel (BD Biosciences, San Jose, California). Following 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... FACS was then used to separate single RFP-positive cells in a 96-well plate using the 561 nm laser on a FACSAriaIII (BD Biosciences, San Jose, CA). Indels at the corresponding target sites in the clones were analyzed by genomic PCR ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated at 90°C in a BBL GasPak™ 100 Holding Jar (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA) to avoid evaporation ...
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bioRxiv - Microbiology 2021Quote: ... coli was suspended in 1 ml of spent supernatant to 0.05 OD600 and inoculated atop solid LB agar in a 6-well plate (BD Biosciences Inc., Franklin Lakes, NJ).
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bioRxiv - Cancer Biology 2022Quote: One hundred thousand human ALL cells were seeded in a volume of 100 μL B cell medium/well (as described above) on Optilux 96-well plate (BD Biosciences, San Jose, CA). EHT1610 ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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bioRxiv - Cancer Biology 2024Quote: ... GFP-positive cells were selected and seeded individually in 96-well plates by flow cytometry (BD FACSAriaTM III, BD Biosciences, Franklin Lakes, NJ, USA). Double SNX1 and SNX2 KO cells were obtained using SNX2 KO cells ...
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bioRxiv - Cell Biology 2022Quote: ... Cells grown on 96 well plates were fixed and permeabilized for 20 mins at 4°C with Fixation/Permeabilization solution (BD Biosciences, cat. no. 554714). Cells were washed 3 times in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml TNF-α or exposed to hypoxia by placing the plate in a BD GasPak™ EZ Anaerobe Pouch System (BD Biosciences, 260683). After 48 hours ...