Labshake search
Citations for Becton, Dickinson and Company :
2001 - 2050 of 3936 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... FAPs were identified as PI-/CD11b-/CD31-/Ter119-/CD45-/Sca-1+ and sorted on a FACS Aria III flow cytometer (BD Biosciences). Freshly sorted FAPs were seeded at a density of 10,000 cells/well into 24-well plates (Ibidi ...
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bioRxiv - Systems Biology 2024Quote: FACS sorting was performed using a BD FACS Symphony S6 or FACSAria III Cell Sorter (BD BioSciences, Franklin Lakes, NJ, USA). The same gating and collection strategy was applied across all time points post lesioning to ensure comparable cell type ratios ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were labelled with negative and positive lineage markers (Table S2) and satellite cells were sorted using a BD FACSAriaTM III (BD Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... Cell sorts were performed by staff at the WEHI FACs facility using a FACSAria III or FACSAria Fusion 9 (BD Biosciences). All data was analysed using FlowJo v10 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... antibody- or PKH26-labelled MDA-MB-231 and 231-R3 cells were washed with PBS twice before sorting by using a FACSAria III Cell Sorter (BD Biosciences). CD44+/CD24- ...
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bioRxiv - Cell Biology 2024Quote: YS0096 strain was derived from YS0095 by switching induction and sorting twice CFP expressing cells (first sorting with Bigfoot Spectral Cell Sorter by Invitrogen, and second sorting with the FACSAria™ III Cell Sorter by BD). YS0096 MAT type was validated by colony PCR (with two sets of primers for the amplification of the MAT locus ...
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bioRxiv - Developmental Biology 2024Quote: ... cardiomyocyte nuclei were sorted based on their PCM1 staining by using a BD FACSAria™ III Cell Sorter (648282, BD Bioscience). Importantly ...
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bioRxiv - Developmental Biology 2024Quote: ... Fluorescent Activated Cell Sorting (FACS) was performed to isolate the GFP+ cells using a BD FACSAria III cell sorter (BD Bioscience). Single cell RNA library construction
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bioRxiv - Bioengineering 2022Quote: ... Brooks Lifesciences) were coated with 200 μL of 2 mg/mL type 1 rat tail collagen (354249, Corning/BD Biosciences) diluted in DMEM equilibrated in 1/10 v/v of 7.5% w/v sodium bicarbonate solution (#25080-060 ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Cell Biology 2020Quote: MLO-Y4 osteocytes (21) were cultured on collagen-coated plates (rat tail collagen type I, BD Biosciences, San Jose, CA) in MEM-a (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... CHO-S wild type cells at a concentration of 1 × 106 cells/mL in a six well plate (BD Biosciences) in AC free media were transfected with a total of 3.75 μg DNA using FreeStyleTM MAX reagent together with OptiPRO SFM medium (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... cells from the BL compartment were collected and cell type and marker expression was determined with a LSRFortessa X-20 (BD). Data were analysed using FlowJo software version 10.6.2 ...
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bioRxiv - Immunology 2024Quote: ... or 5 x 106 wild type HT1080 (HT1080-WT) cells serving as negative control were resuspended in 50% Matrigel (BD) and 50% PBS and subcutaneously injected into the right upper flank of 7-week-old CD1 nude mice (Charles River Laboratories) ...
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bioRxiv - Paleontology 2024Quote: ... Two variants of the ED model can be used according to the type of character involved in the hierarchy: qualitative (ED-QL) and birth-death (ED-BD). They differ in the way the rate matrices are parameterized and whether certain transitions are permitted ...
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bioRxiv - Genomics 2020Quote: ... Staining was performed overnight at 4°C with anti-Pax6-A488 (1:200, BD Biosciences, Cat. N: 561664), anti-Eomes-PE (1:200 ...
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bioRxiv - Systems Biology 2021Quote: ... the membrane was washed once with TBST and incubated with antibodies against N-Cadherin (BD Biosciences, 1:1000), E-Cadherin (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... (E) Median number of PBAF binding events per hub per cell (N=39 WT DMSO cells, 17 ΔBD DMSO cells ...
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bioRxiv - Cell Biology 2021Quote: ... (N=39 WT DMSO cells, 17 ΔBD DMSO cells, 37 WT SAHA cells, and 21 ΔBD SAHA cells). (D ...
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bioRxiv - Cell Biology 2021Quote: ... Intercalated disk images were obtained by labeling fixed M2DCTs with an N-cadherin antibody (1:200, BD Biosciences) and anti-mouse AlexaFluor secondary (ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... N or M peptide pools (10μg/ml) in the presence of anti-CD28 (1μg/ml; BD Biosciences, USA) and recombinant interleukin (IL)-2 (20U/ml ...
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bioRxiv - Immunology 2022Quote: ... and the supernatants were harvested for cytokine detection (IL-10 and TGF-β) by specific ELISAs using a standard protocol (BD OptEIA). The detection limits for IL-10 is 31.3–2000 pg/ml e TGF- β is 62.5–4,000 pg/mL) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 and IFN-γ concentration secreted in cell supernatant were measured using sandwich ELISA according to manufacturer’s protocol (BD Biosciences, USA). Plates were read on a Varioskan Lux multimode microplate reader (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... IFN-γ and TGF-β in mouse serum was performed with the BD OptEIATM Mouse IL-10 ELISA Set (BD Biosciences, 555252), BD OptEIATM Mouse TNF (Mono/Mono ...
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bioRxiv - Neuroscience 2023Quote: ... Concentrations of IL-1β and IL-6 were assayed according to protocol using commercially available mouse IL-1β and IL-6 ELISA (BD Bioscience, Australia). UV absorbance was quantified on a BMG PolarStar microplate reader (BMG Labtechnologies ...
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bioRxiv - Immunology 2024Quote: ... a sample of bulk tank milk before vaccination was also collected and analysed by PCR and ELISA (CIVTEST Bovis BVD/BD p80; HIPRA) by an external laboratory ...
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bioRxiv - Immunology 2021Quote: ... CD62Lhi) from IRF2BP2fl/flLck-cre+ or IRF2BP2fl/flLck-cre- mice were sorted by fluorescence-activated cell sorting (FACS, BD FACS ARIA III) and left unstimulated (data not shown ...
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bioRxiv - Cell Biology 2021Quote: Dissociated hiPSCs were filtered to remove cell aggregates before being clonally isolated using the single-cell deposition function of a BD FACSAria™ III (BD Biosciences). Here ...
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bioRxiv - Microbiology 2020Quote: ... 10,000 cells per sample were analyzed by FACS on a FACSAria III cell sorter (BD Biosciences, 531 nm laser, BP 585/15). Acquired FACS data was analyzed using FlowJo to quantify fluorescent reporter gene expressing cells in each sample set.
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bioRxiv - Cell Biology 2021Quote: ... were then separated according their mAG-geminin intensity (excitation 494 nm/emission 519 nm) using a FACSAria™III cell sorter (BD Biosciences) at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... was added to the cell suspension couple of minutes before the analysis with BD FACSAriaTM III sorter (BD Bioscience, New Jersey, USA).
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bioRxiv - Cancer Biology 2021Quote: ... The cells were sorted into CD45 positive and negative fractions by fluorescence assisted cell sorting (FACS) on a BD FACSAria™ III flow cytometer (BD Biosciences). Positive and negative controls were prepared and used to set up compensations on the flow cytometer ...
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bioRxiv - Immunology 2020Quote: ... from paired BAL fluid and PBMCs were either bulk sorted in 100-cell mini-populations or single-cell sorted using the FACSAria™ III (BD Biosciences) in purity mode into 96-well microplates containing 10 μL of 1% 2-mercaptoethanol RLT buffer (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... cells were analysed using fluorescence-activated cell sorting (FACS) and sorted at 20 cells per well in 96-well plates using a BD FACSAria™ III (BD Biosciences). Two cell phenotypes were sorted ...
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bioRxiv - Cell Biology 2021Quote: ... cell line was enriched by selecting cells expressing EGFP-α-SynA53T by cell sorting with a BD FACS Aria III (BD Biosciences) following amplification.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... a yeast IgG library was subjected to multiple rounds of selection by magnetic activated cell sorting (MACS) and florescence activated cell sorting (FACS, BD ARIA III) in PBS buffer containing 1mM MnCl2 ...
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bioRxiv - Neuroscience 2021Quote: ... and 5 mM EDTA in DPBS and kept cool before undergoing fluorescent activated cell sorting on a FACS Aria™ III (BD Biosciences). Dead cells were excluded from the sort and microglia were selected using CD11b/CD45 double staining and GFP as a marker of infection ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometry and cell sorting were performed with a BD Fortessa Analyzer or a BD FACSAria III sorter (BD Biosciences; (purity ≥ 95%). Flow cytometry analyses were performed using the FlowJo software (Version 10.7).
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bioRxiv - Immunology 2022Quote: ... Flow cytometry and cell sorting were performed with a BD Fortessa Analyzer or a BD FACSAria III sorter (BD Biosciences; (purity ≥ 95%). Flow cytometry analyses were performed using the FlowJo software (Version 10.7).
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bioRxiv - Cell Biology 2023Quote: ... T cells with residual target protein expression were depleted by antibody staining and bead pull-down or cell sorting (BD Aria III). T cells were re-suspended in MACS buffer (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... suspensions were filtered using FACS tube with strainer cap (Falcon 352235) to obtain single cell suspension and live clock neurons were sorted with FACS sorter (BD Aria III). Cells were sorted into 1.5mL DNA low-bind tubes (Eppendorf ...
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bioRxiv - Cancer Biology 2023Quote: ... Sorting was performed at the Magee Womens Research Institute Flow Cytometry Core using a BD FACSAria™ III sorter with FACSDiva software (BD Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Then tdTomato positive and SYTOX Blue negative cells were sorted with fluorescence activated cell sorting (FACS) cell sorters (BD FACSAria Fusion / BD FACSAria III) at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and FACS-enriched for tdTomato-positive cells using a SY3200 Cell Sorter (software WinList3D version 8.0.2) or BD FACSAria III Cell Sorter (BD FACSDiva Software, version 8.0.2) with a 100 µm nozzle ...
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bioRxiv - Neuroscience 2023Quote: ... A 40 μM cell strainer was used for aggregates removal after dissociation and TdTm-expressing RGCs were isolated by fluorescent-activated cell sorting (BD FACS Aria™ III. BD Bioscience). Total RNA extraction was performed with Arcturus Picopure RNA isolation Kit (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... then washed three times with DPBS before permeabilization in ice cold Phosflow Perm Buffer III for 10mins at -20°C (BD Bioscience #558050). Before incubation with primary antibodies ...
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bioRxiv - Biophysics 2023Quote: Cardiac MΦTR were FACS-purified based on cytosolic eYFP fluorescence using an Aria III cell sorter (BD Biosciences, Franklin Lakes, NJ, USA). The gating strategy was based on (a ...
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bioRxiv - Cancer Biology 2023Quote: ... Cas13d-expressing cells with high GFP were further purified by fluorescent activated cell sorting (FACS; FACSAria™ III Cell Sorter; BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Next cells were fixed using BD Cytofix Fixation buffer for 10 minutes at 37°C and permeabilized using ice-cold Perm buffer III (BD Biosciences, 558050) for 30 minutes on ice ...
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bioRxiv - Bioengineering 2022Quote: Engineered cells were cultured for 3-weeks and flow sorted to dispense single GFP-positive cells into individual wells of 96-well plates in a BD FACSAria™ III (BD Biosciences). NALM6-GFP stable cells from a single colony were expanded ...