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Citations for Becton, Dickinson and Company :
2001 - 2050 of 3211 citations for Aldosterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cells were re-suspended in 150 μl of PBS/5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression ...
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bioRxiv - Cell Biology 2020Quote: ... was prepared the day before the experiment by incubating the wells with 50μl/well of 5 μg/ml of anti-integrin α5 antibodies (BD Biosciences, 555651) in 0.05m Na2CO3 pH 9.6 ...
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bioRxiv - Cell Biology 2020Quote: ... The CSCs were selected as the top 5% cells with the highest GFP intensity (Oct4hi) and then sorted with the FACSAria II cell sorter (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... 10% FCS and incubated with the indicated pool of peptides (5 μg/ml final concentration of each peptide) and brefeldin A (1 μg/ml; BD Pharmingen) at 37°C for 12-16 hours ...
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bioRxiv - Genetics 2021Quote: ... and non-neuronal (NeuN−) nuclei were sorted into tubes pre-coated with 5% BSA using a FACSAria flow cytometer (BD Biosciences) equipped with a 100μm nozzle ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples (10,000 events per sample) were analyzed after 5-10 min incubation in the dark on ice on a FACSCalibur flow cytometer (BD Biosciences) in the FL3 channel.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in 1X PBS to remove excess stain with a centrifugation step of 5 min at 2000 ×g and run on a BD FACSCanto cytometer (BD Biosciences). Based on the previous flow cytometry data for establishment of threshold values for gating (Kwon and Smith ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell were resuspended in 200 μl of 2% KSR in PBS and analysed on a 5 laser LSR Fortessa analyser (BD Biosciences). Single cells were gated based on forward and side scatters ...
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bioRxiv - Molecular Biology 2021Quote: ... the top 5% cells expressing the highest levels of CasRx-GFP were sorted using BD FACS ARIA II (BD Bioscience, USA). The CasRx-GFP expressing cells were expanded and screened for CasRx expression using qPCR (primers listed in Supplementary Table S1).
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bioRxiv - Immunology 2021Quote: ... stained with anti-CD117-PECy7 mAb (5 μg.mL−1) and viability dye and sorted by a FACSMelody™ cell sorter (BD biosciences). For immunofluorescence experiments ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4℃ after 1h blocking in 5% skimmed milk (Becton, Dickinson and Company, USA) at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were passaged and cultured under puromycin selection for at least 5 days before flow cytometry analysis using an LSRFortessa X20 instrument (BD Biosciences).
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bioRxiv - Bioengineering 2020Quote: ... at 5:1:1 concentration ratio before transferred into a 1 ml syringe with BD Luer-Lok (BD, 309628, NJ, USA). The syringe was then hanged vertically to allow cell settling by gravity for 10-15 min with no flow ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were incubated with TrypLE Express for 5 min at 37 °C to generate single cell suspensions and strained through a 40 μm mesh (BD Biosciences). They were then fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... 25 μL of cell suspension from each well was washed with 100 μL PBS-EDTA and stained with 5 μL of HLA-DR-FITC (clone G46-6, BD BioSciences) or isotype control (clone G155-178 ...
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bioRxiv - Genetics 2021Quote: ... Cells were passed through a cell-strainer cap on a 5 mL polystyrene round-bottom tube (FALCON 352235) and GFP signal was measured on a BD LSR Fortessa (BD Biosciences) at the Flow Cytometry Facility at the University of California ...
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bioRxiv - Immunology 2021Quote: ... and chemokines (CXCL-10, CCL-2, CXCL-9, CXCL-8 and CCL-5) was quantified by specific cytometric bead arrays (BD Biosciences) on a FACS Canto (BD Biosciences ...
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bioRxiv - Genetics 2019Quote: ... The DAPI-stained cells were resuspended in FACS buffer and then analyzed by Fortessa 4-15 HTS or Fortessa X20 5-18 flow cytometry analyzers (BD Biosciences).
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bioRxiv - Genetics 2020Quote: ... Cells were subsequently washed and resuspended with cold PBS supplemented with 5% FBS and stained with 2 mg/ml Propidium Iodide (BD Biosciences) at room temperature for 10 min ...
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bioRxiv - Genetics 2021Quote: ... The worms were then dissected at the pharynx with a pair of 30G 5/8” needles (PrecisionGlide, BD, Franklin Lakes, NJ). The dissected gonads were fixed with 3% paraformaldehyde (EM Grade ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were implanted subcutaneously in the flank of BALB/c mice using 5 × 106 cells (WEHI-164) with 0.5 ml 29G insulin syringes (MicroFine™+, BD medical). Tumor volume was measured with a caliper and volume was calculated using the formula ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were pelleted at 400xg for 5 minutes and resuspended in 200 µL PBS for immediate analysis with flow cytometry (BD Accuri). Negative/positive nanogel or anti-CD45 signal was established by comparison to unstained cells ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used for analysis of the H2B-GFP LR population (Figure 5): rat anti- Cd74-BUV395 (Clone In-1, BD, 740274), rat anti-Cd45-BUV563 (Clone 30-F11 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed with PBS containing 5 mM EDTA and 3% FBS (FACS Buffer) and then blocked with 1% anti-mouse CD16/CD32 (BD biosciences), 5% normal rat serum and 5% normal mouse serum in FACS buffer for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells or MNC from CML patients or HD were incubated with the following antibodies: 5 µL CD45-PerCP (2D1, BD Biosciences), 2.5 µL CD34-FITC (AC136 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell clusters were washed 3 times with ice-cold HBSS containing 5% FBS and filtered through 100-μm cell strainer (BD Biosciences). The cell suspension containing acini was carefully layered on top of HBSS containing 30% FBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were implanted with 5 x 104 MDA-MB-231 breast cancer cells in 50% cell culture medium (FBS free) and 50% Matrigel (BD biosciences) by subcutaneous injection in the left dorsal flank ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were again pelleted at 500xg for 5 min at 4°C then resuspended in Cytofix/Cytoperm reagent (BD Biosciences) and incubated on ice for 30 min Following fixation/permeabilization ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were incubated for 25 mins at 37°C 5% CO2 and then fixed and permeabilized with CytoFix/CytoPerm (BD Biosciences) at room temperature for 20 mins ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Microbiology 2021Quote: ... 5-10 Spheroids resuspended in serum-free medium were seeded in an insert that has been coated with Matrigel (BD Biosciences). Lower compartment was added with medium containing 20% FBS ...
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bioRxiv - Microbiology 2021Quote: ... 20µL were transferred into 180µL of 10mM Tris-1mM EDTA (for DiOC2(3) staining) or PBS (for DiSC3(5) staining) and analyzed by flow cytometry using LSR Fortessa (BD Bioscience) operated by FACS Diva software (BD Bioscience) ...
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bioRxiv - Microbiology 2021Quote: ... Salivary glands were cleanly dissected from infected mosquitoes placed into 5 µl of L-15 on a MaTek well (MaTek #P35G-0-14C) followed by the addition of 5 µl of matrigel (BD #356231). The matrigel was allowed to polymerize at room temperature for ∼10 minutes and Z-stacks of salivary glands were acquired using a 3i spinning disk confocal microscope (Zeiss Axio Observer Z1 microscope with Yokogawa CSU222 spinning disk) ...
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bioRxiv - Molecular Biology 2021Quote: ... between 500.000 and 1 million GFP-mCherry double positive cells were sorted each day for 5 consecutive days using two cell sorters (BD FACSAriaII SORP and BD FACSAriaIII). Sorted cells were pooled ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were transferred to a 50 ml tube and 9 ml LB (10 g/l Peptone (OXOID, LP0037), 10 g/l NaCl (ACROS, 207790010) and 5 g/l Yeast Extract (BD, 211929)) were added with 50 μg/l kanamycin (ACROS ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... overlapping peptides (10µg/mL) or media alone for 60 hours at 37 °C and 5% CO2 with proliferation dye (Violet Proliferation Dye, BD Biosciences). Peptides were generated in 15mer format with 8 amino acid overlaps for the ID91 fusion antigen ...
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bioRxiv - Microbiology 2022Quote: ... were grown in in Pro99 media supplemented with 5 g L-1 peptone and 1 g L-1 yeast extract (BD Difco). All heterotrophs were grown at 24 °C ...
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bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
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bioRxiv - Immunology 2022Quote: ... cells were washed 2x with FACs buffer at 1000xg for 5 minutes at room temperature and analyzed on a LSR II (BD Biosciences) at the Flow Cytometry Shared Resource at the University of Arizona ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 5-6 days and CFSE dilution of T cells was determined on a BD Canto II (BD Bioscience) and analyzed by FlowJo (v10.1 ...
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bioRxiv - Cell Biology 2019Quote: Huh-7 cells were taken from the xCELLigence wells at defined times along the different proliferation curves and their position in the cell cycle was evaluated by measuring 5-bromo-2-deoxyuridine (BrdU) incorporation using the APC BrdU Flowkit (catalogue number 552598, BD Pharmingen). Cells were incubated with BrdU (10 mM ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in 3ml FACS buffer before being fixed in 1% paraformaldehyde and analyzed within 5 days using a Fortessa Flow Cytometer and BD FACSDiva Software (BD Biosciences). Flow cytometric data were analyzed using FlowJo™ (Treestar ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... T cells were analyzed for proliferation at day 5 via Cell trace dye (eBioscience) and inhibitory receptor expression (LAG3 and PD1) on a Fortessa II (BD Bioscience) and data analyses were performed via FlowJo (Tree Star).
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bioRxiv - Cancer Biology 2020Quote: ... and the fraction of sgRNA-expressing cells was measured 3 days and 5 days after treatment as BFP-positive cells by flow cytometry on an LSR-II (BD Biosciences). Specifically ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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bioRxiv - Immunology 2021Quote: ... Spleen cells of immunized mice were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 mAb (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... Blood samples were diluted 1:5 with PBS and 10 µl aliquots were added to 1 ml of BD Retic-Count reagent (BD Biosciences). After a 60 min incubation in the dark at RT ...