Labshake search
Citations for Becton, Dickinson and Company :
2001 - 2050 of 2336 citations for 7 Chloro quinazoline 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... were routinely grown at 37 °C under anaerobic conditions in a Bactron III-2 anaerobic chamber (Shellab, USA) in BHIS medium: 3.7% weight vol−1 brain heart infusion broth (BD, USA) supplemented with 0.5% weight vol−1 yeast extract (BD ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed 2 times with flow buffer and incubated with BD Cytoperm™ Permeabilization Buffer Plus (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... rat anti-mouse IL-12p40/p70 (10 μg/mL, clone C17.8), rat anti-mouse IL-2 (10 μg/mL, clone S4B6) (all from BD Biosciences), were included in the cultures ...
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bioRxiv - Neuroscience 2020Quote: ... or formalin solution (2% in 1x PBS) in the plantar surface of the right hind paw using a standard 28G insulin syringe (BD) and video recorded from below for either 15 or 60 minutes respectively ...
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bioRxiv - Microbiology 2022Quote: ... Live cells were measured every 2-3 days on the BD FACS Celesta Flow Cytometer (BD Biosciences, Franklin Lakes, NJ) using a known amount of spike-in AccuCount Blank particles (Spherotech ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells then were trypsinized and resuspended in PBS containing 2% FBS and dTomato-positive cells were sorted using a BD FACSAria III flow cytometer (BD Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
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bioRxiv - Immunology 2024Quote: ... and then stained for 30 min at 4°C in PBA buffer (PBS, 0.5% BSA, 2 mM EDTA) in the presence of Fc Block (1:50 dilution, BD-Biosciences), using the following antibody combination ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: Twenty µl of platelet post activation supernatants were incubated with 2.5 µl of phycoerythrin (PE)-conjugated mouse anti-human CD62P (clone AK-2, BD Biosciences), 2.5 µl of PE-conjugated CD61 (clone VI-PL2 ...
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bioRxiv - Pathology 2024Quote: ... Lentivirally transduced LX-2 cells were sub-cultured until confluent before being flow-sorted on the BD FACSAriaTM (BD Biosciences) to obtain cells with top 10 % highest eGFP expression ...
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bioRxiv - Immunology 2024Quote: ... Cytokine staining was performed with 2 x 106 cells after 3.5h of in vitro stimulation in Opti-MEM media supplemented with Golgiplug (BD Biosciences), 10 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Microbiology 2024Quote: ... Samples were blocked for 30 minutes in FACS buffer (2% FBS 1X PBS) containing Fc Block anti-CD16/32 (2.4G2, BD Biosciences) (1:100 dilution) ...
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bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
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bioRxiv - Cell Biology 2023Quote: Cytokine released by the SARS-CoV-2-infected or ApoBD-treated MDMs were measured using human cytokine bead array as per manufacturer’s instruction (BD Biosciences). Briefly ...
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bioRxiv - Immunology 2023Quote: ... stained cells were resuspended in PBS 2% FBS and DC1 were purified in a FACS Aria II Cell Sorter (BD).
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bioRxiv - Immunology 2023Quote: ... LLC or CULA cells (2 × 105) resuspended into 50 ul DMEM and enriched with 20% extracellular matrix (Matrigel, BD Biosciences), were intrapleurally injected to the left lung lobe of mice using a 29G needle (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Polyclonal goat anti-mouse Ig conjugated to APC was used as a secondary antibody (2 μg/mL, BD Pharmigen #550826). For all antibodies ...
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bioRxiv - Microbiology 2023Quote: ... FITC-A between 2×104–106) or an LSR-Fortessa flow cytometer (BD Biosciences; for Fig 5A and Fig S5C) and data was plotted in FlowJo (ver ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were sorted in PBS + 2 % FBS using a BD FACSAriaTM II or BD FACSArisa Fusion (both from BD Biosciences) cell sorters ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H ...
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bioRxiv - Immunology 2023Quote: Bone marrow was isolated from dissected femurs that were gently crushed in PBS with 2% (v/v) FBS and subsequently filtered over a 70μm cell strainer (BD Falcon). Red cell lysis was done by incubating samples with ACK lysis buffer (Quality Biological ...
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bioRxiv - Cancer Biology 2023Quote: Spleens and livers were harvested into ice cold FACS buffer (PBS supplemented with 2% FCS v/v) and then emulsified through a 70μM filter (BD Biosciences), centrifuged and washed in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... Staining was quenched by washing in FACS buffer (PBS with 2 mM EDTA and 2% FCS) after which cells were incubated with anti-CD16/32 (Fc block, clone 2.4G2, BD Biosciences) for 15 minutes at 4°C to prevent non-specific binding ...
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bioRxiv - Developmental Biology 2023Quote: ... one million cells were transferred in 100 μl of FACS buffer supplemented with 0.5μM Y27632 and then added 2 μl Fc Block (BD Pharmingen, 553141) per sample followed by 10 min incubation on ice ...
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bioRxiv - Microbiology 2023Quote: ... 25 μL of control serum and 25 µL of serum pooled from all mice within a treatment group were pipetted onto a 2-mm diameter plain paper disc (BD) and allowed to dry for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... For microscopy experiments SDC-lysine media was used (2% glucose, 6.75 g/l yeast nitrogen base without amino acids (291929, BD Difco), 1.92 g/l yeast synthetic drop-out media supplements without lysine (Y1896 ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 2 × 106 vector control or shACSL4 cells were mixed 1:1 by volume with Matrigel (BD Biosciences). Each mouse was injected orthotopically into both the flanks ...
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bioRxiv - Microbiology 2023Quote: ... yeast isolates were grown overnight for 16 h from a single colony in 2 mL PD broth (BD, Sparks MA) with shaking at 225 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Leukemic cell infiltration was evaluated using immuno-labeling with anti-huCD45 and anti-huCD7 antibodies (see Supplementary Table 2) and a FACS-Canto II flow cytometer (Becton Dickinson, BD). Cells from Femur ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: The titles and abstracts of 2 341 articles retrieved from the preliminary search were screened and cross-checked by two independent reviewers (ZL and BD) and discrepancies were resolved through discussion with a third researcher (DM) ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with pre-optimized concentrations of the following antibodies: PE anti-human IgD (clone IA6-2, BD Biosciences), PE-TXRD anti-human CD10 (clone HI10A ...
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bioRxiv - Immunology 2023Quote: ... The protein binding capacity of the well surface was saturated with 300 μL blocking solution (PBS supplemented with 2% skim milk powder (BD) and 0.1% Tween 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... Immune cells were resuspended in surface staining mix containing FACS buffer with 1/2 of final volume of Brilliant Stain Buffer (BD), 50 μg/ml anti-mouse CD16/CD32 (2.4G2 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 × 106 cells were harvested and resuspended in 100 μl of ice-cold 20% matrigel (BD Bioscience, Franklin Lakes, NJ) in PBS (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... in the presence of 100 U/ml of human IL-2 and soluble anti-CD28 antibody (BD Biosciences, Clone: 37.51). T lymphoblasts were cultured at 37 °C in 5% CO2 for 3 days in exponential growth phase ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lifted by pipetting in FACS buffer (D-PBS, 2% FBS, 0.1% sodium azide) and analyzed on a FACSCelesta (BD Biosciences). At least 20,000 events were collected per sample on the cytometer ...
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bioRxiv - Microbiology 2024Quote: ... were incubated on ice for 30 min in the dark in 50 ul of PBS containing 2 ul of APC-labeled mouse anti-human CD4 or anti-human CD184 (CXCR4) antibody (BD biosciences 561840 and 560936 ...
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bioRxiv - Immunology 2024Quote: ... and stored in FB 4C overnight before analysis on a 3 laser Aurora spectral flow cytometer (Cytek, figure 1D, Supplementary Figure 2) or X20 Special Order 5-lasers Fortessa (BD) flow cytometer (other figures) ...
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bioRxiv - Immunology 2024Quote: ... We resuspended cells in FACSWash (1x PBS, 2% FBS, 0.2% sodium azide) and acquired events on a LSRFortessa (BD Biosciences), which we analyzed using FlowJo v10 (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... cells were suspended in PBS containing 0.5% FBS and 2 mM EDTA and were sorted with a 100-μm nozzle using BD FACSAria III (BD Biosciences). Sorted cells were pelleted and lysed in RNA lysis buffer (Zymo Research).
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bioRxiv - Molecular Biology 2020Quote: ... The samples were then stained for 30min at 4°C with the following antibodies: monoclonal anti-human CD33 V450 (WM53; BD Biosciences, San Jose, CA, USA); HLA-ABC FITC (W6/32 ...
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bioRxiv - Immunology 2021Quote: ... or PMA + ionomycin in the presence of brefeldin A for 4 h and stained using the Cytofix/Cytoperm Kit (BD Bioscience, Franklin Lakes, NJ, USA). Foxp3-positive cells and apoptotic cells were detected using the BD Foxp3 Staining Kit (BD Bioscience ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... Cells were then incubated for 1h at RT (or left at 4°C overnight) in the Iridium-intercalator solution in fixation and permeabilization buffer (BD Cytofix/Cytoperm™, BD Biosciences). Then cells were washed with 1x permeabilization buffer (BD Perm/Wash™ ...
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bioRxiv - Immunology 2019Quote: ... Cells were stained for 30 min at 4°C under darkened conditions with the following fluorochrome labeled monoclonal antibodies: anti-CD4-AmCyan (Clone SK3, BD Biosciences, Franklin Lakes, Nj, USA), anti-CD25-APCCy7 (Clone M-A251 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed with PBS and incubated for 20 hours at 4°C in primary antibody solution 1:3000 mouse anti-Ki67 (BD Biosciences, San Jose, CA, USA), 2% normal horse serum ...
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bioRxiv - Immunology 2023Quote: ... The resulting single-cell suspensions were used at a concentration of 4 million cells/mL and stained with Fixable Viability Dye eFluor 780 (BD Bioscience, 565388, San Jose, USA), followed by Fc Block incubation and labelling with fluorochrome-conjugated antibodies (CD45-PerCP-Cy5.5 ...
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bioRxiv - Microbiology 2023Quote: ... Bladder cells were concentrated to 4 x 105 /mL and 1 mL was dispensed into a 6-well sterile culture dish (BD Falcon; 35 x 18 mm2) and incubated at 37°C with 5% CO2 for 24 h ...
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bioRxiv - Microbiology 2023Quote: ... purified gametocytes from DFsc7 or DFsc7;ΔRbpm1 lines were suspended in GMB at 4°C and sorted using a BD FACS AriaIII instrument (BD Biosciences, San Jose, CA, USA) based on their GFP and mCherry fluorescence intensity for male and female gametocytes ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were resuspended in 500 m 2% FBS in HBSS and incubated on ice in the dark with 1 l Fc block (BD Biosciences) for 3 minutes ...