Labshake search
Citations for Becton, Dickinson and Company :
2001 - 2050 of 7852 citations for 6 Chloro 2 methyl 1 2 4 triazolo 4 3 b pyridazin 3 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: Mice received an intraperitoneal injection of BrdU (BrdUrd; 2 mg in 200 μL PBS; BD Biosciences) or PBS only at 0 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were stained with specific antibodies (Supplementary Table 2) and analyzed on FACS Canto II (BD) as described (Sontag et al. ...
-
bioRxiv - Immunology 2021Quote: Monoclonal antibodies (mAbs) used for multiparameter flow cytometry (LSR Fortessa 2, BD Biosciences, San Diego, CA) and gating strategy to identify the different leukocyte subpopulations was done as previously described (21 ...
-
bioRxiv - Genomics 2021Quote: ... Cells that presented high CD163 levels were sorted using an Aria 2 Flow Cytometer (BD Biosciences) in order to obtain a homogeneous M2 population (Hu et al. ...
-
bioRxiv - Genomics 2020Quote: ... Cells from patients 2 through 5 were stained following washing with anti-EPCAM-PE (347198, BD Biosciences;1:50 dilution in ice-cold phosphate-buffered saline ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then pelleted and resuspended in 2 mL 1X BD FACS lysing solution (#349202, BD) for 12 min to achieve fixation and permeabilisation.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then pelleted and resuspended in 2 mL 1X BD FACS lysing solution (#349202, BD) for 12 min to achieve fixation and permeabilisation.
-
bioRxiv - Evolutionary Biology 2022Quote: ... virilis and 5mm diameter for others) filled with 5% sucrose (Wako) and 2% Bacto agar (BD) on one side ...
-
bioRxiv - Immunology 2022Quote: ... Cells were washed and stained with an antibody cocktail against CD3 (clone SP34-2, BD Biosciences), CD4 (clone OKT4 ...
-
bioRxiv - Immunology 2022Quote: 48-well plates were coated with antibodies directed against murine CD3 (clone: 500A.2, BD Biosciences) and CD28 (clone ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked with either Armenian Hamster α-mouse CD16-2 antibody (clone 9E9; BD Biosciences) or rat α-mouse CD16/CD32 antibody (clone 2.4G2 ...
-
bioRxiv - Microbiology 2023Quote: ... 5% CO2 for 2 h prior to the addition of Brefeldin A and monensin (BD Biosciences). After being left in culture overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the active form of ⍺ IIbβ3 integrin (Emfret Analytics, JON/A-PE, #M023-2, and BD Biosciences anti-human PAC-1-FITC ...
-
bioRxiv - Microbiology 2023Quote: ... a 2 mL frozen aliquot of the library was inoculated in 200 mL LB (BD#244620) with 25 ug/mL of kanamycin and grown at 30 °C overnight ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then fixed in 2% PFA and acquired with an LSR-II cytometer (BD Biosciences).
-
bioRxiv - Cancer Biology 2023Quote: A suspension of 2 × 103 cells in a growth medium containing 0.33% Noble agar (BD Biosciences) was plated onto 6-well plates precast with 0.5% Noble agar ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells from patients 2 through 16 (P2-P16) were stained with anti-EPCAM-PE (347198, BD Biosciences;1:50 dilution in ice-cold phosphate-buffered saline ...
-
bioRxiv - Cancer Biology 2019Quote: ... A pure polyclonal population of dCas9-KRAB expressing cells was generated by 3 rounds of fluorescence activated cell sorting (FACS) gated on the top half of BFP positive cells (BD FACS Aria II).
-
bioRxiv - Cell Biology 2021Quote: ... by subcutaneous injection of 3 × 105 A375 or H2122 cells in RPMI-1640 medium supplemented with 50% Matrigel (BD Biosciences, cat. no. 354234). Seven days after the injection ...
-
bioRxiv - Cancer Biology 2019Quote: ... A polyclonal population of cells expressing all 3 fluorescent proteins (mturquoise2, Venus and mCherry) was subsequently generated by two rounds of FACS (BD FACS Aria II).
-
bioRxiv - Immunology 2021Quote: ... were resuspended in defRPMI-1640 with 3% newborn calf serum and Benzonase™ (FACS buffer) in 5 mL FACS tubes and pre-incubated with GolgiStop™ (BD Biosciences) for ∼60 min at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... Following overnight incubation the plate was washed 3 times with DPBS and then incubated with 20 µl anti-CD45 antibody (BD Pharmingen, catalog #550539) in 12 ml of 0.2% BSA for a minimum of 2 hours ...
-
bioRxiv - Immunology 2023Quote: ... Mice were inoculated subcutaneously (50uL) with 3×104 PFU or injected intracranially (10uL) with 50 PFU of LGTV-TP21 using insulin syringes (BD Medical, #BD-329461). At appropriate times post infection ...
-
bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and consecutively sorted using a SONY SH800S or a FACS Aria Cell Sorter (BD Biosciences) into NCAM1- and NCAM+ populations ...
-
bioRxiv - Cancer Biology 2021Quote: ... for 30 minutes at 4°C and sorted into 384-wells SORTseq plates using a FACS Aria Cell Sorter (BD Biosciences) and directly stored at −80°C until further processing.
-
bioRxiv - Microbiology 2020Quote: ... washed with cold PBS by centrifugation (5 min, 550g at 4°C) and analysed with a FACS BD AccuriTM C6 (BD Biosciences) instrument ...
-
bioRxiv - Cell Biology 2020Quote: ... half of the medium (50 μl) per well was replaced with fresh differentiation medium containing 4% (v/v) Matrigel (BD Bioscience). On day 3 ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were incubated for 30 min in the dark at 4°C after adding relevant antibodies (BD biosciences, Franklin Lakes, NJ). A FACSCalibur system (BD biosciences ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
-
bioRxiv - Cell Biology 2022Quote: Cells harvested at different time points of reprogramming were stained in FACS buffer for 30 min at 4°C and washed with FACS buffer prior to acquisition with LSR Fortessa (BD Biosciences) cytometer ...
-
bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
-
bioRxiv - Immunology 2021Quote: ... Binding of mAbs to CTLA-4 on the cell surface was determined using LSR II (BD Biosciences, San Jose, CA, USA) or CytoFLEX LX (Beckman Coulter ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences). For subsequent limiting dilution cloning ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4℃ after 1h blocking in 5% skimmed milk (Becton, Dickinson and Company, USA) at room temperature ...
-
bioRxiv - Developmental Biology 2020Quote: ... cells were fixed in 4% paraformaldehyde for 30 minutes and stained with PE-conjugated or purified CD45 antibody (eBioscience, 30-F11 or BD Biosciences) to ascertain the generation of haematopoietic progeny ...
-
bioRxiv - Microbiology 2019Quote: ... transduced cells were sorted for single positive mCherry signal or double positive mCherry/eGFP signal at 4°C using FACS Aria III and the corresponding FACS Diva software (BD Bioscience). Cells were sorted from HBSS supplemented with 10 µM Y-27632 ...
-
bioRxiv - Genomics 2021Quote: The titer of the lentivirus containing either the genome-wide library or distal sub-library of gRNAs was determined by transducing 5×105 cells with varying dilutions of lentivirus and measuring the percentage of GFP-positive cells 4 days later using the Accuri C6 flow cytometer (BD Biosciences).
-
bioRxiv - Genomics 2021Quote: ... tissues were blocked (3% bovine serum albumin in 5% normal goat sera and 0.1% Triton) and incubated overnight at 4°C with primary antibodies: CD31 (Neomarkers, RB-10333-P0) or CDH1 (BD Biosciences, 6315829). Slides were washed and incubated with Cy3-goat-anti-mouse or anti-rabbit-conjugated secondary antibodies (Jackson Immunoresearch Laboratories ...
-
bioRxiv - Genetics 2019Quote: ... The DAPI-stained cells were resuspended in FACS buffer and then analyzed by Fortessa 4-15 HTS or Fortessa X20 5-18 flow cytometry analyzers (BD Biosciences).
-
bioRxiv - Immunology 2021Quote: ... the killing capacity of NK cells was tested when they coculture with senescent / young or modulated for ST8SIA1 expression MRC5.NK cells are added to the culture for 4 h in presence of monensin and brefeldin (BD Biosciences) at the effector/target ratio of 1:1 ...
-
bioRxiv - Bioengineering 2020Quote: Cell surface staining was performed at 4 °C for 30 min and was analysed using a FACSCalibur flow cytometer (BD Biosciences) and CellQuest Software (BD Biosciences) ...
-
bioRxiv - Bioengineering 2020Quote: Cell-surface staining was performed for 45 min at 4 °C and was analyzed using a FACSCalibur flow cytometer (BD Biosciences). A minimum of 1 × 104 events per sample were examined.
-
bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
-
bioRxiv - Biophysics 2020Quote: ... Cells were washed once and fluorescence intensities were quantitated using a 4-laser Fortessa flow cytometer (BD Bioscience, San Jose, CA). To correct the fluorescence intensity difference of the two antibodies ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were again pelleted at 500xg for 5 min at 4°C then resuspended in Cytofix/Cytoperm reagent (BD Biosciences) and incubated on ice for 30 min Following fixation/permeabilization ...
-
bioRxiv - Microbiology 2021Quote: ... fixed with 4% formaldehyde for 15 min and washed and resuspended in FACS buffer before analyzing by flow cytometry using FACSCelesta (BD Biosciences) or Cytek Aurora spectral analyzer (Cytek Biosciences) ...
-
bioRxiv - Microbiology 2021Quote: ... Selection of the positive cells was performed using 4 µg/ml puromycin and viable cells were expanded for flow cytometry cell sorting using a FACSMelody (BD Biosciences) equipped with a 561 nm excitation laser and 613/18 filter ...
-
bioRxiv - Synthetic Biology 2021Quote: Streptomyces coelicolor M1146 and derivative strains were routinely maintained on Soya-Mannitol Flour (SFM) agar supplemented with 10 mM MgCl2 and International Streptomyces Project medium #4 (ISP4) (BD Difco) at 30°C as described previously [34] ...
-
bioRxiv - Bioengineering 2022Quote: ... Genes encoding the proteins were cloned into pET29b between the restriction sites NdeI and XhoI and expressed for 4 h in Terrific Broth (BD Difco) using Lemo21 (NEB ...
-
bioRxiv - Physiology 2022Quote: ... Cell suspensions were washed and re-suspended in PBS at 4°C and analysed using a BD 5L LSR Fortessa and BD FACSDiva software (BD Biosciences). To exclude dead cells ...