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Citations for Becton, Dickinson and Company :
1951 - 2000 of 2077 citations for Recombinant Human IFNA5 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... and human tumor cells were collected as the mouse MCH class I-negative fraction using flow cytometry (BD FACSAria, Becton Dickinson). Then ...
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bioRxiv - Pathology 2021Quote: Cells were fixed in 2% PFA for 15 minutes at room temperature and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight as previously described 36 ...
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bioRxiv - Immunology 2021Quote: Cells were co-cultured in R10 medium alone or in presence of K562 cells for 6 hours in the presence of anti-human CD107a (H4A3, FITC, BD Biosciences). Golgi plug and Golgi stop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: Supernatants were collected after 4 or 24 h and the levels of TNF and IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... or with no target cells (or an E:T of 1:0) and cultured overnight before being staining with allophycocyanin (APC)-conjugated anti human-CD69 antibody (BD Biosciences #555533). Flow cytometry was performed using a BD- Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... T cells from different human donors (P1, P2, P3) were isolated using the RosetteSep™ negative isolation method and activated (using BD Human T cell activator beads coated with anti-CD3 and anti-CD28 ...
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Genomics 2020Quote: Immune cells for bulk mRNA sequencing were incubated with Fc Block for 20 minutes and then stained with one of six panels of directly conjugated antibodies for 30 minutes: anti-human CD16 (BD 558122), CD123 (BD 560826) ...
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bioRxiv - Immunology 2019Quote: ... cDNA was amplified - 10 cycles for resting cells and 9 cycles for in vitro activated cells - using the Human Immune Response primer panel (BD Biosciences), containing 399 primer pairs ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IFN-γ ELISA set (Cat no. – 555138) and anti-human IL-10 ELISA set (Cat no. – 555157) were purchased from BD Biosciences ...
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bioRxiv - Genomics 2021Quote: Healthy human hiPSC cell lines for Figure 4 were cultured under feeder-free conditions on plates coated with human-qualified Matrigel (BD Biosciences) diluted 1:40 in mTeSR-1 medium (StemCell Technologies) ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cancer Biology 2022Quote: ... and engraftment of human cells in the bone marrow and spleen was measured with flow cytometry (CD45-PE-Cy7 antibody, BD Biosciences). Single-cell suspensions were prepared from the bone marrow ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolation of CD4+ T cells was carried out by negative selection using the Human CD4 T Lymphocyte Enrichment Set-DM (BD Biosciences). All patient samples were obtained with informed consent for research use ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary AML cells were harvested and stained with human CD45 and cells were analyzed by a high-throughput automated plate reader (BD LSRFortessa). Dead cells were excluded using the Near-IR Live/Dead marker (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: Human and Non-human primate samples were processed similarly: Blood was collected in 8 mL sodium citrate-containing CPT tubes (BD Biosciences) and separated into plasma and peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Neuroscience 2022Quote: ... and tumor necrosis factor alpha (TNFα) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). A LEGENDplex™ HSC Myeloid Panel kit (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... human cells were stimulated with PMA and ionomycin (500 ng/mL, eBioscience) in the presence of GolgiPlus and GolgiStop (BD Biosciences) for 4 h at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
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bioRxiv - Microbiology 2023Quote: ... for 16 hours followed by stimulation with a peptide pool of a broad range of HLA-subtypes and infectious agents (CEFX Ultra SuperStim Pool; JPT) (1 µg/ml) and 1 μg/ml mouse-anti-human CD28 antibody (#556620; BD Biosciences), or IL-2 (20 IU/ml ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then spun down and resuspended in 100LμL of buffer containing fluorescently conjugated antibodies for one hour (5LμL of each antibody per sample): APC-Cy7 Mouse Anti- Human CD4 (1:20 dilution; BD Biosciences, #557871), APC-Cy7 Mouse IgG1 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 2x with 1% FBS in PBS and incubated in 1% FBS in PBS with APC-conjugated mouse anti-human CD68 (BD Biosciences) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... bone marrow aspirates were performed every 6 weeks post-transplant and human chimerism was tracked by flow cytometry analysis (using a BD LSR Fortessa analyzer or BD FACSymphony) and analyzed with the following ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognises residues 91-99 from the NAC region of human α-synuclein (20)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Cell Biology 2023Quote: ... and tumor necrosis factor (TNF) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). Readings were done on an AttuneTM Nxt Flow Cytometer.
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bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... with the same panel of human antibodies as used for cell sorting and analyzed by flow cytometry on a FACS Fortessa (BD Biosciences). UltraComp eBeads (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 10 µl of this bacterial suspension was added to the wells of a sterile 96-well flat-bottomed plate and incubated with 90 µl of freshly-donated human blood (collected in EDTA-treated tubes; BD Biosciences). Blood from each of four individual volunteers was assayed twice ...
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bioRxiv - Pathology 2023Quote: ... then incubated for 20 minutes at 4°C with a combination of the following mouse anti-human antibodies: AF488 anti-CD3 (clone UCHT1, BD Biosciences), PE anti-CD203c (clone NP4D6 ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for 20 min a 4°C with the appropriate combinations of fluorochrome-conjugated anti-human antibodies for the identification of CD8 T cells and sub-populations: CD3 BV480 (clone UCHT1, BD Biosciences), CD8 BV421 (clone RPA-T8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were further analyzed for TNFα and/or IFNγ production using respectively BD OptEIA Human TNFα and IFNγ ELISA kits (BD Biosciences). Flat-bottom 96-well plates (COSTAR ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently hLSECs were purified via FACS by labelling with mouse anti-human CD31-FITC conjugated antibody (clone WM-59, BD Biosciences). Two independent preparations of transplanted iEC were used for single cell RNAseq ...
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bioRxiv - Cell Biology 2023Quote: ... PECAM was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD31 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: T cells were staining 30 min at 4°C with anti-human CD3-PE (Cyto Matin Gene, CMGCD3-P100) anti-CD3-PerCP (BD, 347344), anti-CD3-FITC (BD ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Immunology 2023Quote: ... BM aspirate samples from heathy donors using a Ficoll density gradient and stained with the following anti-human antibodies: IgD-Brilliant Violet 480 (Cat. No. 566138; BD Biosciences), CD3-BUV737 (Cat ...
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bioRxiv - Immunology 2023Quote: ... Cytokine concentrations in the supernatants of DC cultures were measured using a BD human inflammatory cytometric bead array kit (BD #551811) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: mouse anti-human CD3 conjugated to BUV737 (clone UCHT1, BD, 612750), mouse anti-human CD45 conjugated to FITC (clone HI30 ...
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bioRxiv - Cell Biology 2024Quote: ... IL-6 and IL-1β cytokines were measured in the cell culture supernatant after 2 h of co-exposure using CBA human Inflammatory cytokine kit (BD Biosciences), while chemokines CXCL8/IL-8 ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: Specificity of neoantigen-reactive CD8+ T-cells and TCR-transduced T-cells was assessed by human IFNγ (BD Biosciences Cat. #555142), IL-2 (BD Biosciences Cat ...
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bioRxiv - Neuroscience 2023Quote: ... and tumor necrosis factor (TNF) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). Concentrations of chemokines in cell supernatants were measured using the LEGENDplexTM Human Proinflammatory Chemokine Panel 1 (Biolegend) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences) and infected with HIVBaL at 0.01 ng/106 cells ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... and stained for 30 min at 4 °C with the following antibody mixture: PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Fixed cells were stained for 30 min at 4° C with PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... was used to isolate the neutrophil population (verified by flow cytometry using an APC-cy7 antibody specific for human CD16 (clone 3G8, BD Biosciences) and a PE antibody for human CD66b (clone C10F5 ...