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Citations for Becton, Dickinson and Company :
151 - 200 of 235 citations for TRIF Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... A primary antibody dilution was prepared in the blocking solution with the antibodies diluted 1:200 for HIF1α (BD Bioscience, 610958), 1:50 for phosphor-S6 (CST ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice and resuspended in 200 μL of blocking buffer in the presence of 7-AAD (BD Biosciences Cat#: 51-68981E) or DAPI (Invitrogen Cat# ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were washed 2 times in FCM Blocking Buffer then analyzed on a BD Accuri C6 Flow Cytometer (BD Biosciences).
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bioRxiv - Microbiology 2021Quote: Spleen and liver cells were incubated (20 min, 4°C) with anti-Fc-gamma blocking antibody (clone 2.4G2) (BD Biosciences, Belgium) and were subsequently stained with fluorescent conjugated antibodies (30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... the cells were resuspended with blocking buffer (5% BSA in DPBS) and stained with PE-Cy7 rat anti-CD31 (1:400, 561410, BD biosciences), APC mouse anti-EpCAM (1:400 ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2×106 cells were incubated for 15 min at +4°C with blocking buffer (1xPBS, 1% BSA, 2mM EDTA) containing 5mg/ml α-CD16/CD32 (2.4G2, BD Biosciences). For surface staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with fluorescent-conjugated anti-mouse antibodies after blocking CD16/CD32 with Fc Block (BD Biosciences, San Jose, CA). For intracellular staining ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Synapsin (1:500) and anti-CD133 (1:500) were used after Fc blocking with CD16/32 antibody (553141; BD Pharmingen). Respective Secondary antibodies ...
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bioRxiv - Immunology 2020Quote: Cells isolated from the organs indicated in figure 4 and figure S9 were incubated with an Fc blocking agent (BD Pharminogen) for 10min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescent labelled anti-mouse antibodies after blocking CD16/CD32 with Fc Block (BD Biosciences, San Jose, CA). Flow cytometry analysis was performed using following antibodies ...
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bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... At the end of the co-culture the cells were washed twice and incubated with human or mouse Fc receptor blocking antibodies (BD Biosciences). Human macrophages were stained with anti-CD11b (M1/70 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then harvested and incubated in a blocking buffer containing 10%FBS and 1μg/mL Human Fc block (BD Biosciences, USA) for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with 2% ECL prime blocking reagent in PBS-T (Amersham; #RPN418) and incubated against AP-2 (mouse-anti-AP50, BD, #A611351) and GAPDH (rabbit-anti-GAPDH ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions were stimulated with OVA peptide (SIINFEKL) 2µM 37°C for 4 hrs in the presence of Golgi Stop (monensin, BD Biosciences). Upon extracellular staining ...
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bioRxiv - Immunology 2021Quote: ... no stimulation and a peptide pool spanning the spike protein at a concentration of 1 μg ml−1 of each peptide in the presence of 1 μg ml−1 of anti-CD28 (clone CD28.2, BD Biosciences) and anti-CD49d (clone 9F10 ...
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bioRxiv - Immunology 2021Quote: ... live cells were separated by density gradient and then restimulated by incubation with irradiated APCs from C57BL/6 mice that had been pre-loaded with indicated concentrations of SIINFEKL peptide or by PMA (10ng/ml) and ionomycin (1μg/ml) in the presence of GolgiPlug (BD Biosciences) for 4 hours.
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bioRxiv - Immunology 2019Quote: ... Each obtained monomeric H-2Db/peptide complex (0.5 mg/ml) was tetramerized at a 4:1 ratio with streptavidin-PE (BD Biosciences).
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bioRxiv - Neuroscience 2022Quote: ... aged 8 weeks received subcutaneous injections to both flanks and base of the tail of MOG35-55 peptide (125μg total, Mimotopes) emulsified 1:1 (vol/vol) in Freund’s complete adjuvant (BD Cat# 263810) containing additional Mycobacterium tuberculosis (5mg/mL ...
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bioRxiv - Immunology 2020Quote: ... 1–2 × 106 cells were incubated with 1 μM of SARS-CoV-2 minimal epitope or pool of up to ten epitopes (1 μM/peptide) for 9 h at 37°C in the presence of protein transport inhibitor (GolgiPlug; BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Spleen and lung cells that were stimulated overnight with peptide pools (as described under ex-vivo stimulation and intracellular staining) were fixed and permeabilized with Cytofix/Perm solution (BD) and then stained for intracellular cytokines ...
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bioRxiv - Immunology 2021Quote: ... and I53-50B components of the NP-scaffold (1.2 µg/ml of each peptide) in the presence of 1 µg/ml of anti-CD28 (clone CD28.2, BD Biosciences) and anti-CD49d (clone 9F10 ...
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bioRxiv - Microbiology 2022Quote: EAE was induced by injecting 8 to 10-week-old female C57BL/6J mice with 150 mg MOG35-55 peptide (Genemed Synthesis) emulsified in complete Freund’s adjuvant (CFA) (BD Difco) per mouse subcutaneously in the flanks ...
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bioRxiv - Bioengineering 2022Quote: ... CD8+ T cells were stimulated with specific or irrelevant peptides at 5μg/mL (JPT Technologies) in the presence of Golgi Plug (BD Biosciences) in complete medium (RPMI medium supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Immunology 2024Quote: Splentocyte samples were either aliquoted in mFACS Buffer for staining or resuspended in mR10 and stimulated ex vivo with LCMV peptide DbGP33 (0.2 μg/mL) in the presence of Golgi Plug and Golgi Stop (BD Bioscience) for 5 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: Lymphocytes isolated from the spleen or siIEL compartment of naïve QFLTg_WT or OT-1_WT chimera mice were cultured in vitro with 2µM of FL9 or SL8 peptide respectively together with GolgiPlug (BD Biosciences) for 4.5h ...
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bioRxiv - Biochemistry 2023Quote: The coding sequences of SAP05 or SAP05 mutants excluding signal peptides were amplified and ligated into Gateway vector pDEST-GBKT7 (BD). Full length sequences of A ...
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bioRxiv - Microbiology 2024Quote: ... were stimulated with peptide pools of ESAT-6 and CFP-10 (10μg/ml of each peptide pool) in the presence of Brefeldin A (GolgiPlug, BD Biosciences) at 37°C/5% CO2 for 3.5-4 hours prior to staining ...
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bioRxiv - Neuroscience 2024Quote: EAE was induced in 8- to 10-week-old female C57BL/6J mice by injecting 250 μg of MOG35-55 peptide (Genemed Synthesis) emulsified in complete Freund’s adjuvant (CFA) (BD Difco) per mouse ...
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bioRxiv - Neuroscience 2024Quote: ... a 1:1 mixture of 200 µg of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG 35-55; Scrum) in saline and complete Freund’s adjuvant (231131, BD Difco) containing 6 mg/ml Mycobacterium tuberculosis H37Ra (231141 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated with individual CHIKV peptide pools (2μg/ml) for 6 h in the presence of BD GolgiPlug (BD Bioscience). Cells were harvested and stained with antibodies for CD3 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated in media containing 10 µg/mL blocking antibody (Purified Rat Anti-Human CD29 Clone Mab 13, BD Biosciences, #552828) or equivalent concentration non-targeting isotype control (Purified Rat IgG2a κ Isotype Control Clone R35-95 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were counted using a hemacytometer and then treated with an Fc blocking buffer containing anti-Cd16/32 (BD Biosciences, 1:500) for 20 minutes at 4 degrees Celsius before a 30 minute incubation with antibody master mixes for the myeloid or lymphoid panels at 4 degrees Celsius ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 μL of PBS containing 5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: Intestinal organoids were encapsulated into boronate ester hydrogels and placed into differentiation media containing 10 μg/mL of blocking antibody (anti integrin α6 antibody BD Pharmingen #555734 or anti integrin β4 antibody BD Pharmingen #553745). At 48 hours samples were fixed and immunostained as described above.
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bioRxiv - Microbiology 2020Quote: ... the isolated cells were incubated with mixtures of consecutive 15mer peptides (overlapping by 11AA) of the Mtb Tb6Ag in the presence of the costimulatory molecules CD28 and CD49d (BD Biosciences) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were re-stimulated with 1 µg/mL of master peptide pools in the presence of brefeldin A (GolgiPlug; BD Bioscience) and monensin (GolgiStop ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions were stimulated with short peptides for 5h in the presence of brefeldin A or with long peptides for 8h of which the last 6h in presence of brefeldin A (Golgiplug; BD Pharmingen). Flow cytometric acquisition was performed on a BD Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Immunology 2022Quote: Lymphocytes were isolated from the lungs of VSV-OVA-challenged mice and stimulated ex vivo with OVA peptide 1 mM) in the presence of Golgi-Plug (BD Biosciences) for 5 hours ...
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bioRxiv - Immunology 2022Quote: ... and the remaining cells were incubated for 5-6 hrs at 37°C in the presence of 1 uM of the indicated peptide and brefeldin A (GolgiPlug; BD Pharmingen). Surface staining was done overnight at 4 degrees ...
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bioRxiv - Plant Biology 2021Quote: ... The AtCGL160 CDS without the signal peptide (see Supplemental Table S2 for primer information) was cloned into the bait vector pGBKT7 (BD-AtCGL160), whereas the coding sequences of CF1-α ...
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bioRxiv - Immunology 2021Quote: ... Spleen cells of immunized mice were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 mAb (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... spleen cells of immunized mice were incubated with 50 μM of each peptide for 6 hours at 37°C in the presence of monensin (GolgiStop, BD Biosciences) and 0.8 μg of FITC-conjugated anti-mouse CD107a mAb (BioLegend) ...
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bioRxiv - Microbiology 2019Quote: ... or with stimulation by a total PCS peptide pool (PCS1-12, Table S11) were transferred to 12 × 75mm3 polystyrene tubes (BD Falcon) and washed with FACS wash (PBS supplemented with 2% FBS) ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Immunology 2020Quote: ... lung T cells were incubated at 37°C for 5 hours with 5 μM Bl-Eng2 or calnexin peptide and 1μg anti-mouse CD28 (BD, cat 553294). After 1h ...
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bioRxiv - Immunology 2020Quote: ... splenocytes and tumor-infiltrating cells were cocultured with 1 μmol/L of AdpgkMut or AH1 peptide in the presence of Brefeldin A (BD Biosciences) for 5h in vitro ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...