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Citations for Becton, Dickinson and Company :
151 - 200 of 261 citations for PDGF Receptor Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Fc receptors were blocked using the anti-mouse CD16/CD32 (FcBlock clone 2.4G2) monoclonal antibodies (BD Biosciences, #553141, 1/400) and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies ...
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bioRxiv - Immunology 2019Quote: ... The number of CD11b receptors on CD45+CD11b+ TAMCs was quantified using the QuantiBRITE PE Fluorescence Quantitation Kit (BD Biosciences), according to the manufacturer’s recommended protocol.
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bioRxiv - Cell Biology 2020Quote: ... 3×106 cells were incubated with purified rat IgG2b anti-mouse CD16/32 receptor antibody as per supplier’s instructions (Mouse BD Fc-block, clone 2.4G2, BD Biosciences, 553141) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: Surface staining was done in the presence of anti-CD16/32 antibodies to block FcγRII/III receptors using Mouse Fc blockTM (BD Biosciences). Cells were incubated with the Mouse Fc blockTM for 10 min followed by incubation with fluorochrome-conjugated pre-titrated antibodies for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells were lysed using ammonium chloride and Fc receptors were blocked using anti-mouse CD16/CD32 (BD Bioscience) before staining.
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution, BD Biosciences, for the spike ELISA) for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... Peroxidase activity was detected using 3,3’ Diaminobenzidine substrate (BD Pharmingen #550880). Slides were counterstained with hematoxylin ...
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bioRxiv - Immunology 2022Quote: ... Plates are developed with the OptEIA TMB substrate reagent set (BD).
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bioRxiv - Immunology 2020Quote: ... streptavidin-HRP and 3-amino-9-ethylcarbazole (AEC) substrate (BD Bioscience) were added and spots counted using a CTL ImmunoSpot S6 Universal Analyzer (CTL) ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS-T and TMB substrate (BD Bioscience) was added ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and developed using AEC Substrate set (BDBiosciences #551951). Spots were recorded with AID Elispot Reader and enumerated manually.
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bioRxiv - Immunology 2021Quote: ... then developed with 3-Amino-9-ethylcarbazole (AEC) substrate (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The color was developed using an AEC substrate reagent (BD Biosciences), and the spots were counted using an ELISPOT reader (AID ELISPOT Reader ver ...
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bioRxiv - Immunology 2023Quote: ... plates were developed with TMB substrate kit (BD Biosciences, Oxford, UK) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed and OptEIA TMB substrate (BD Biosciences Cat # 555214) was applied for 8 ...
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bioRxiv - Immunology 2023Quote: ... Development of spots was performed using AEC substrate kit (BD, AUS) as per manufacturer’s instructions and manually counted to determine ASCs.
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bioRxiv - Microbiology 2024Quote: ... tetramethylbenzidine (TMB) substrate solution and hydrogen peroxide (BD, San Diego, USA) were added to the plates for 30 min in the dark ...
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bioRxiv - Immunology 2022Quote: BMDMs were harvested as described before and Fc receptors were blocked with an anti-mouse CD16/CD32 antibody (1:250, BD Biosciences) in FACS buffer (5 % heat-inactivated FCS ...
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bioRxiv - Neuroscience 2021Quote: ... Fc receptors were blocked as described above and cells were stained with CD3e FITC (1:200, clone 145-2C11, BD Pharmingen) or CD3e PE-Cy7 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... and their Fc receptors were blocked for 10 min at 4 °C with anti-CD16/CD32 antibodies (10 μg/mL: BD Biosciences). After blocking ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked with Purified Rat Anti- Mouse CD16/CD32 Mouse BD Fc Block™ (2.4G2, BD Pharmingen, 1:1000). Cell surface antigens were stained in PBS containing 2 % (vol/ vol ...
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bioRxiv - Immunology 2019Quote: ... Cells were initially stained with fluorochrome-conjugated antibodies against surface receptors (see Table S1) in BD Brilliant stain buffer (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... T cells were analyzed for proliferation at day 5 via Cell trace dye (eBioscience) and inhibitory receptor expression (LAG3 and PD1) on a Fortessa II (BD Bioscience) and data analyses were performed via FlowJo (Tree Star).
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bioRxiv - Immunology 2020Quote: ... For flow cytometry analysis first Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were subsequently washed and stained for following surface markers with directly conjugated antibodies against CD19/B220 (PerCP Cy5.5 or eFluor 450) ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... encoding CXCR4 and CXCR7 were transfected into U87 cells and cells stably expressing the receptors were obtained following puromycin selection and subsequent single-cell sorting using BD FACSAria II cell sorter (BD Biosciences) (61) ...
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bioRxiv - Cancer Biology 2019Quote: ... and antibody to Fc receptor binding inhibitor (eBioscience) before incubation with the following primary antibodies: PE-conjugated anti-human CD31 (BD Pharmingen), PE-conjugated anti-human CD45 (BD Pharmingen) ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting pellet was resuspended in 1 ml of complete neurosphere medium containing epidermal growth factor (EGF, 20 ng/ml; receptor grade, BD Biosciences) and basic fibroblast growth factor (bFGF ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Bioengineering 2023Quote: ... At the end of the co-culture the cells were washed twice and incubated with human or mouse Fc receptor blocking antibodies (BD Biosciences). Human macrophages were stained with anti-CD11b (M1/70 ...
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bioRxiv - Cell Biology 2023Quote: ... the purity of CD4 T cells expressing the OTII T cell receptor was confirmed by flow cytometry (BD LSRFortessa Cell Analyzer) after staining with Anti-CD4-Alexa647(BD bioscience,557681 ...
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bioRxiv - Immunology 2023Quote: ... Single cells were then washed with 1% Bovine serum albumin (BSA) in PBS and blocked with anti-Fcγ receptor (BD Biosciences). Human peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Immunology 2022Quote: ... The plates were washed and TMB substrate solution (Cat# 555214, BD Biosciences) was added to the wells for color development ...
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bioRxiv - Immunology 2022Quote: ... Enzymatic revelation was performed with the TMB substrate reagent set (BD OptEIA). All Antibodies used are indicated in Supplementary table 3.
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bioRxiv - Immunology 2021Quote: ... plates were developed with 100 µL/well TMB substrate solution (BD, 555214) for 15 min and stopped with 50 µL/well 2N H2SO4 ...
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bioRxiv - Evolutionary Biology 2021Quote: HRP activity was detected with 3,3’,5,5’-Tetramethylbenzidine (TMB) Substrate Reagent (BD Biosciences #555214 ...
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bioRxiv - Biochemistry 2021Quote: ... Detection was performed using a two-component peroxidase substrate kit (BD biosciences) and the reaction was stopped with the addition of 2N Sulfuric acid ...
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bioRxiv - Immunology 2020Quote: HRP activity was detected with 3,3′,5,5′-Tetramethylbenzidine (TMB) Substrate Reagent (BD Biosciences #555214 ...
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bioRxiv - Microbiology 2021Quote: ... The 3-amino-9-ethycabazole (AEC) substrate kit was purchased from BD Pharmingen.
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bioRxiv - Microbiology 2020Quote: ... Plates were washed and developed with TMB substrate reagent (BD Biosciences, USA). Reactions were stopped with 0.5M hydrochloric acid and absorbances was measured at 450nm on a microplate reader ...
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bioRxiv - Immunology 2021Quote: ... The wells were washed 7 times with PBS and TMB substrate (BD) was added (100 μl/well) ...
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bioRxiv - Molecular Biology 2022Quote: ... TMB substrate was prepared according to the manufacturer’s instructions (BD OptEIA™) and 100 µl/well was applied to the plate before incubating for 10 min ...
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bioRxiv - Immunology 2024Quote: ... wells were washed and 50µL of TMB Substrate Reagent (555214, BD Biosciences) was added for revelation step ...
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bioRxiv - Immunology 2023Quote: ... Peroxidase reactions were developed using OptEIA TMB substrate reagent set (BD Biosciences) and stopped with sulfuric acid ...
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bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells expressing the relevant surface receptor or fluorescence protein were sorted using a BD FACSMelody or a BD FACSAria SORP Cell Sorter (BD Biosciences, USA). Luc2 and NLuc expression was confirmed by exposing cells seeded in the wells of a flat-bottom white 96-well plate to 1 volume of 15 mg/mL D-luciferin (#LUCNA-1G ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...