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Citations for Becton, Dickinson and Company :
151 - 193 of 193 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... from pDONR207 into the Y2H vector pPC97-GW to be expressed as a fusion protein with the GAL4 DNA-binding domain (GAL4-BD). AH109 yeast cells (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... Gene sequences without predicted introns were inserted into the vectors pGAD24 and pGBKT7 generating translational fusions to the Gal4 activation domain (AD) and DNA-binding domain (BD), respectively.
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with 50µg/ml propidium iodide and the DNA content was analyzed by flow cytometry (BD FACSAria III).
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bioRxiv - Microbiology 2021Quote: ... The same 16S 515F/806R primers (described above) were used in qPCR (described above) to determine density shifts in the peak DNA of buoyant density (BD) for each incubation ...
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bioRxiv - Cell Biology 2022Quote: ... was added to cell suspension to stain DNA 5 min before flow cytometry analysis which was conducted on BD FACSCanto II Cell Analyzer (BD). Data is analyzed using FlowJo software.
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bioRxiv - Immunology 2020Quote: BMDM viability following different stimuli was assessed using the fluorescent DNA intercalator 7-aminoactinomycin D (7-AAD, BD Biosciences, USA). Briefly ...
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bioRxiv - Plant Biology 2021Quote: ... between the NdeI or NcoI and BamHI restriction sites in order to express N-terminal fusion proteins with the GAL4 DNA binding domain (BD) or with the GAL4 activation domain (AD) ...
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bioRxiv - Microbiology 2019Quote: Cell cycle phase distribution was determined by analysis of DNA content via either flow cytometry (BD FACS Canto™ II) or imaging flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... DNA dye was removed by a simple wash in PBS/sucrose and samples analysed on a FACS Canto (BD Biosciences) using processed uninfected RBCs as negative control ...
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bioRxiv - Microbiology 2021Quote: ... from pDONR207 into the yeast two-hybrid vector pPC97-GW for expression in fusion downstream of the GAL4 DNA-binding domain (GAL4-BD). The control siRNA and a pool of TAX1BP1 siRNA (Ambion ...
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bioRxiv - Plant Biology 2022Quote: ... while the full-length coding sequence of HlMYB7 was cloned into the prey vector pGADT7-GW [DNA-binding domain (BD)] (Addgene Inc ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA of strain B1 was extracted from the cells after 3 days of growth in Marine Broth 2216 (MB, BD) using a bacterial genomic extraction kit (SaiBaisheng ...
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bioRxiv - Plant Biology 2022Quote: ... and the PCR products were fused into the activation domain (AD) vector pGADT7 and/or the DNA-binding domain (BD) vector pGBKT7 and verified by sequencing ...
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bioRxiv - Immunology 2022Quote: ... the cells were analyzed by bromodeoxyuridine (BrdU) incorporation and DNA content staining with APC BrdU Flow Kit (BD Biosciences, #552598) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the amplified PGRP3 cDNA was digested with BamHI and EcoRI and ligated to a pGBKT7 vector containing the GAL4 DNA-binding domain (BD). Successful recombinant pGBKT7-PGRP3 bait construction was verified with restriction digestion (BamHI ...
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bioRxiv - Cell Biology 2023Quote: ... of BicDR were cloned into pOAD and pOBD2 vectors so that they were in frame with the activator domain (AD) or the DNA binding domain (BD)13,30 ...
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bioRxiv - Immunology 2023Quote: HD11 viability following different stimuli was assessed using the fluorescent DNA intercalator 7-aminoactinomycin D (7-AAD, BD Biosciences, USA). 7-AAD assay allows identifying populations representing live ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length or truncation (ΔTM) of TbPEX15 with and without mutations were cloned in the pPC97 vector containing GAL4- DNA Binding Domain (BD) as described in Table 1 ...
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bioRxiv - Biochemistry 2024Quote: ... AtHSP90-3 and ASDURF were transferred to the pGADT7-GW and pGBKT7-GW destination vectors using Gateway LR Clonase II enzyme mix to produce fusion proteins of the Gal4-activation domain (AD) and GAL4 DNA-binding domain (BD), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were co-stained with 4′,6-diamidino-2-phenylindole (DAPI) to measure DNA content and analyzed with a BD LSRFortessa flow cytometer (BD Biosciences) and FlowJo software (TreeStar).
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bioRxiv - Cancer Biology 2019Quote: ... The DNA content of cells and cell cycle distribution were analyzed by Flow cytometric analysis on BD LSRFortessa cell analyzer (BD Biosciences).
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bioRxiv - Genomics 2020Quote: ... and collected in both PAXGene Blood DNA tubes (761115, PreAnalytix) and ‘purple top’ EDTA (ethylenediaminetetraacetic acid) Vacutainer tubes (367863, BD Biosciences). Blood samples were stored at 4C upon arrival and processed within 2 days ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 10 minutes at room temperature and DNA content analysed by flow cytometry on a BD FACSCalibur (BD Biosciences). The data were analysed with FlowJo.
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bioRxiv - Cancer Biology 2021Quote: ... The levels of DNA-loaded MCM were detected by incubating cells in anti-MCM2 BM28 antibody (1:200, BD biosciences, 610700) for 1 hr at 37°C in the dark followed by incubation in anti-mouse-488 secondary antibody (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... were transformed with one plasmid derived from pPC86 (GAL4-activation domain, AD (‘Prey’)) and pPC97 (GAL4-DNA-binding domain, BD (‘Bait’)) (Chevray and Nathans ...
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bioRxiv - Cell Biology 2022Quote: ... Cell survival was measured by FACS analysis after staining cells with a membrane-impermeable DNA binding dye (DAPI, PI, or 7-AAD) and/or annexin V (BD Pharmingen).
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bioRxiv - Genetics 2021Quote: The cellular DNA content was analyzed by propidium iodide staining following ethanol fixation and flow cytometry on a FACSCanto II (BD Biosciences) instrument ...
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Baculoviruses were produced using homologous recombination in insect cells by co-transfection with linearized baculoviral DNA and the transfer vector pAcGP67A (BD Biosciences), which carries an N-terminal gp64 signal peptide for secretion ...
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bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...
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bioRxiv - Bioengineering 2024Quote: ... Flow cytometry analysis of enucleated or viable cells was performed using double-stranded DNA dyes (DRAQ5, 65-0880-96, Invitrogen and 7AAD, 559925, BD, respectively).
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bioRxiv - Biochemistry 2023Quote: ... 1 µg of this genomic DNA from soluble fraction was mixed with 3 µg of anti-IdU/BrdU antibody (3D4 clone, BD Science) in BrdU-IP buffer composed of PBS and 0.0625% Triton X-100 overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were sorted for DNA content on a FACSAria Fusion sorter and flow profiles were recorded with the FACSDiva software (BD Franklin Lakes).
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bioRxiv - Molecular Biology 2021Quote: ... QT3 and QT4 were processed by Flow Cytometric Analysis with a Cycletest PLUS DNA Reagent Kit (BD Biosciences, Le pont de Claix). Number of replicates per group are provided in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA extraction and BrdU-labelled DNA immunoprecipitation were performed with anti-BrdU antibody (40 μL of 25 μg mL−1, BD Pharmingen, #555627). Validation of BrdU immunoprecipitation was carried out using qRT-PCR on known Early (BMP1 ...
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bioRxiv - Microbiology 2019Quote: Metabolic BrdU labeling and detection of newly synthesized DNA were performed by adding 10 µM BrdU (APC BrdU Flow Kit, BD Pharmingen, # 557892) for one hour prior to harvest ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DNA content was analyzed by flow cytometry using a 488 nm laser (BD FACS Verse with software BD FACSuite™, version 1.0.6.). Cell cycle distribution was analyzed using ModFit LT (Verity Software House ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with anti-Annexin V antibodies and propidium iodide (PI, DNA marker dye) as per protocol supplied with kit (BD biosciences; 556547). Flow cytometry analysis was performed for annexin V+/PI-(apoptotic ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA content analysis for cell cycle stage assessment was performed by FACS using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA). Three independent biological replicate cell cultures and cell treatments were performed.
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bioRxiv - Cell Biology 2023Quote: ... Samples were analyzed by flow cytometry for DNA content and EdU labelled cells using a BD LSR Fortessa Laser Cell Analyser (BD Biosciences, Erembodegem, Belgium) equipped with 5 excitation lasers (UV 355nm ...
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bioRxiv - Cell Biology 2019Quote: ... Suspended cells were fixed using 70% ethanol and kept at −20°C for at least 24 hours prior to measuring DNA content using propidium iodine (PI) with RNAse (BD Biosciences, San Jose, CA). Approximately 50,000 cells were centrifuged at 1300 rpm for 5 minutes at 22°C ...
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bioRxiv - Genetics 2019Quote: ... and DNA profiles were evaluated using fluorescence-activated cell sorting with a FACSCalibur flow cytometer and Cell Quest software (BD Biosciences, San Jose, CA).
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bioRxiv - Cancer Biology 2023Quote: ... The DNA content of the cells was measured by flow cytometry using a 488 nm laser (BD FACSVerse with BD FACSuite software, version 1.0.6.). The cell cycle distribution was analysed with ModFit LT (Verity Software House ...