Labshake search

151 - 200 of 10000+ citations since 2020

• Enhanced feature matching in single-cell proteomics characterizes response to IFN-γ and reveals co-existence of different cell states

  Karl K. Krull, Syed A. Ali, Jeroen Krijgsveld, bioRxiv - Systems Biology 2024

  Quote: ... and immediately administered to the cell sorting via a BD FACSAria III instrument (BD Biosciences), utilizing a 384-well plate configuration ...
• Proteome-scale tagging and functional screening in mammalian cells by ORFtag

  Filip Nemčko, et al., bioRxiv - Systems Biology 2024

  Quote: ... The remaining cells were sorted for GFP-positive (Activator screens) or GFP-negative (Repressor screens) populations using BD FACSAria III or IIu cell sorters (BD Biosciences) and processed for mapping of genomic integrations (see below) ...
• Proteome-scale tagging and functional screening in mammalian cells by ORFtag

  Filip Nemčko, et al., bioRxiv - Systems Biology 2024

  Quote: ... reporter expression was analyzed on an LSR Fortessa (BD) flow cytometer ...
• Retention of ES cell-derived 129S genome drives NLRP1 hypersensitivity and transcriptional deregulation in Nlrp3^−/− mice

  Felix D. Weiss, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were stained in the presence of Fc Block (553142, BD Biosciences).
• Retention of ES cell-derived 129S genome drives NLRP1 hypersensitivity and transcriptional deregulation in Nlrp3^−/− mice

  Felix D. Weiss, et al., bioRxiv - Immunology 2024

  Quote: ... Ly-6G (553127, BD Pharmingen)) ...
• Retention of ES cell-derived 129S genome drives NLRP1 hypersensitivity and transcriptional deregulation in Nlrp3^−/− mice

  Felix D. Weiss, et al., bioRxiv - Immunology 2024

  Quote: For identification of peritoneal cavity cell subsets the following markers were used: CD45-PE/Cy7 (552848, BD Biosciences), CD11b-BV510 (101245 ...
• Retention of ES cell-derived 129S genome drives NLRP1 hypersensitivity and transcriptional deregulation in Nlrp3^−/− mice

  Felix D. Weiss, et al., bioRxiv - Immunology 2024

  Quote: ... CD16/32-PerCP/Cy5.5 (560540, BD Pharmingen), and CD34-AF647 (128606 ...
• Retention of ES cell-derived 129S genome drives NLRP1 hypersensitivity and transcriptional deregulation in Nlrp3^−/− mice

  Felix D. Weiss, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were stained in the presence of Fc Block (553142, BD Biosciences).
• The physiology of bud dormancy and cell cycle status in grapevine

  Dina Hermawaty, et al., bioRxiv - Plant Biology 2024

  Quote: The nuclear DNA content was measured using a BD FACSCanto™ II (BD Biosciences, San Jose, CA) flow cytometer equipped with an air-cooled 488-nm solid state 20 mW laser ...
• Matrix confinement modulates 3D spheroid sorting and burst-like collective migration

  Grace Cai, et al., bioRxiv - Biophysics 2024

  Quote: ... a mouse anti-E-cadherin antibody at 1:2000 (610181, BD Biosciences), and a primary rabbit anti-vimentin antibody at 1:1000 (D21H3 ...
• Enhanced cardiac mitochondrial biogenesis by nitro-oleic acid remedies diastolic dysfunction in a mouse model of heart failure with preserved ejection fraction

  Marion Müller, et al., bioRxiv - Pathology 2024

  Quote: ... and a mouse Alexa Fluor 647-conjugated antibody to TnT (#565744; BD Pharmingen, 1:100). Nuclei were stained with DAPI (1:1000 ...
• A unique subset of pericystic endothelium associates with aberrant microvascular remodelling and impaired blood perfusion early in polycystic kidney disease

  Daniyal J Jafree, et al., bioRxiv - Pathology 2024

  Quote: ... rat monoclonal anti-CD31 (BD Biosciences ...
• Enhanced cardiac mitochondrial biogenesis by nitro-oleic acid remedies diastolic dysfunction in a mouse model of heart failure with preserved ejection fraction

  Marion Müller, et al., bioRxiv - Pathology 2024

  Quote: ... and troponin T (1:100, 565744, BD Pharmingen) were used ...
• Unsupervised segmentation of 3D microvascular photoacoustic images using deep generative learning

  Paul W. Sweeney, et al., bioRxiv - Biophysics 2024

  Quote: ... and matrigel (BD). For MCF7 ...
• Megakaryocyte maturation involves activation of the adaptive unfolded protein response

  M. Faiz, et al., bioRxiv - Cell Biology 2024

  Quote: ... Antibodies used were anti-CD61 phycoerythrin (PE) (1:20; 1075384; BD Biosciences), mouse IgG1 PE (isotype control ...
• Megakaryocyte maturation involves activation of the adaptive unfolded protein response

  M. Faiz, et al., bioRxiv - Cell Biology 2024

  Quote: ... mouse IgG1 PE (isotype control; 7223589; BD Biosciences), anti-CD41a V450 (1:20 ...
• Synthetic coevolution reveals adaptive mutational trajectories of neutralizing antibodies and SARS-CoV-2

  Roy A. Ehling, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... FACS (BD FACSMelody Cell Sorter) was performed to isolate PnP cells for antibody surface expression and display ...
• Synthetic coevolution reveals adaptive mutational trajectories of neutralizing antibodies and SARS-CoV-2

  Roy A. Ehling, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... FACS (BD FACSAria Fusion Cell Sorter / BD FACSMelody / Sony MA900 ...
• Synthetic coevolution reveals adaptive mutational trajectories of neutralizing antibodies and SARS-CoV-2

  Roy A. Ehling, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... Yeast cells expressing RBD that maintained antibody-binding (IgG+/FLAG+) or showed a complete loss of antibody binding (escape) (IgG-/FLAG+) were sorted by FACS (BD Aria Fusion or BD Influx). Collected cells were cultured in SD-UT medium for 16-48 hours at 30°C ...
• Synthetic coevolution reveals adaptive mutational trajectories of neutralizing antibodies and SARS-CoV-2

  Roy A. Ehling, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... FACS (BD FACSAria Fusion Cell Sorter / BD FACSMelody / Sony MA900) was performed on PnP cells expressing surface antibody and binding to soluble RBD protein ...
• Synthetic coevolution reveals adaptive mutational trajectories of neutralizing antibodies and SARS-CoV-2

  Roy A. Ehling, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... or showed a complete loss of antibody binding (escape) (IgG-/FLAG+) were sorted by FACS (BD Aria Fusion or BD Influx). Collected cells were cultured in SD-UT medium for 16-48 hours at 30°C ...
• Synthetic coevolution reveals adaptive mutational trajectories of neutralizing antibodies and SARS-CoV-2

  Roy A. Ehling, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... Binding (ACE2+/FLAG+) and non-binding (ACE2-/FLAG+) yeast cells were sorted by FACS (BD FACSAria Fusion). Collected cells were immediately checked for purity and resorted if necessary.
• MXRA8 promotes adipose tissue whitening to drive obesity

  Wentong Jia, et al., bioRxiv - Physiology 2024

  Quote: ... The 2X stain cocktail included the following antibodies (final dilutions are all 1:300 unless otherwise indicated): rat anti-mouse SiglecF-BV421 (1:400, clone E50-2440, cat# 562681 BD Horizon), rat anti-mouse/human CD11b-Pacific Blue (clone M1/70 ...
• MXRA8 promotes adipose tissue whitening to drive obesity

  Wentong Jia, et al., bioRxiv - Physiology 2024

  Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
• MXRA8 promotes adipose tissue whitening to drive obesity

  Wentong Jia, et al., bioRxiv - Physiology 2024

  Quote: ... An equal volume of 2X stain cocktail was made in Brilliant Stain Buffer (BD Biosciences) and added on top ...
• Retention of ES cell-derived 129S genome drives NLRP1 hypersensitivity and transcriptional deregulation in Nlrp3^−/− mice

  Felix D. Weiss, et al., bioRxiv - Immunology 2024

  Quote: ... Isolated cells were centrifuged (350g, 5 minutes) and resuspended in 1X Red Blood Cell Lysis solution (555899, BD Bioscience) for 15 minutes at RT ...
• Reduced mitochondrial transcription sensitizes acute myeloid leukemia cells to BCL-2 inhibition

  Laleh S. Arabanian, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 7-AAD (BD Biosciences) was used for dead cell exclusion ...
• Reduced mitochondrial transcription sensitizes acute myeloid leukemia cells to BCL-2 inhibition

  Laleh S. Arabanian, et al., bioRxiv - Cancer Biology 2024

  Quote: For RNAseq experiment BM samples were FACS sorted using BD FACS Aria fusion (BD Biosciences). Human AML cells were isolated using human CD45 antibody ...
• The ideotype for drought tolerance in bioenergy Populus nigra

  Hazel K Smith, et al., bioRxiv - Plant Biology 2024

  Quote: ... The dry volume (DV, m3) was measured by water displacement (Berta et al., 2010) and basic density (BD, kg m-3) calculated as reported by Carriero et al ...
• Loop-mediated Isothermal Amplification (LAMP) assay for reliable detection of Xanthomonas axonopodis pv. vasculorum

  Mitchell Marabella, et al., bioRxiv - Plant Biology 2024

  Quote: ... including nutrient agar (NA) (BD, Becton Dickinson) glucose NAG media (NA medium supplemented with 0.4% glucose) ...
• A Trisomy 21-linked Hematopoietic Gene Variant in Microglia Confers Resilience in Human iPSC Models of Alzheimer’s Disease

  Mengmeng Jin, et al., bioRxiv - Neuroscience 2024

  Quote: ... onto Matrigel (BD Biosciences)-coated culture plates at a ratio of 1:4∼1:8 supplemented with ROCK inhibitor Y-27632 (10 mM ...
• ADP-dependent platelet activation is required for thrombus formation during a long-distance flight

  Julie Tourn, et al., bioRxiv - Pathology 2024

  Quote: ... and a rat anti-mouse CD16/CD32 (clone 2.4G2, BD Biosciences) were used A rat anti-mouse CD45 coupled with APC (clone 30-F11 ...
• Lineage-tracing of Acta2+ cells in aged mice during lung fibrosis formation and resolution supports the lipofibroblast to myofibroblast reversible switch

  Arun Lingampally, et al., bioRxiv - Pathology 2024

  Quote: ... was added to cell suspension to exclude dead cells and sorting was carried out using FACSAria III cell sorter (BD Biosciences). Data were analyzed using FlowJo software (FlowJo ...
• ADP-dependent platelet activation is required for thrombus formation during a long-distance flight

  Julie Tourn, et al., bioRxiv - Pathology 2024

  Quote: ... a rat anti-mouse Ly6C labeled with PC7 (clone AL-21, BD Biosciences) and a rat anti-mouse CD16/CD32 (clone 2.4G2 ...
• An arrayed CRISPR/Cas9 screen identifies mTORC1 as a regulator of lipid droplet accumulation in APOE E3 and APOE KO iPSC-derived microglia

  Sonja Meier, et al., bioRxiv - Neuroscience 2024

  Quote: ... and PE anti-human CD81 antibody (BD Pharmingen) for expression analysis and were measured on a CytoFLEX LX flow cytometer (Beckman Coulter).
• ADP-dependent platelet activation is required for thrombus formation during a long-distance flight

  Julie Tourn, et al., bioRxiv - Pathology 2024

  Quote: A rat anti-mouse LY6G antibody labeled with PE (clone 1A8, BD Biosciences), a rat anti-mouse GPIb-beta Dylight 649 (clone X649 ...
• ADP-dependent platelet activation is required for thrombus formation during a long-distance flight

  Julie Tourn, et al., bioRxiv - Pathology 2024

  Quote: ... and a rat anti-mouse Ter-119 labeled with PE (clone Ter-19, BD Biosciences) were used for both labelling of circulating cells and to study the thrombi composition ...
• Lateral olivocochlear neurons modulate cochlear responses to noise exposure

  Austen A. Sitko, et al., bioRxiv - Neuroscience 2024

  Quote: ... Mouse IgG1 anti-Ctbp2 (BD Biosciences 612044, 1:500); Goat Anti-Chat (EMD Millipore AB144P ...
• Brain-wide neuronal circuit connectome of human glioblastoma

  Yusha Sun, et al., bioRxiv - Cancer Biology 2024

  Quote: ... mouse anti-KI67 (BD Biosciences, 550609, 1:500), rabbit anti-KI67 (Abcam ...
• Trabectedin Enhances Oncolytic Virotherapy by Reducing Barriers to Virus Spread and Cytotoxic Immunity in Preclinical Pediatric Bone Sarcoma

  Emily M. Ringwalt, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-Ly6C (BD Biosciences), and anti-Foxp3 (eBiosciences ...
• Trabectedin Enhances Oncolytic Virotherapy by Reducing Barriers to Virus Spread and Cytotoxic Immunity in Preclinical Pediatric Bone Sarcoma

  Emily M. Ringwalt, et al., bioRxiv - Cancer Biology 2024

  Quote: ... California) except for anti-CD25 (BD Biosciences, San Jose, CA), anti-Ly6C (BD Biosciences) ...
• Polymeric nanoparticles delivery of AMPK activator 991 prevents its toxicity and improves muscle homeostasis in Duchenne Muscular Dystrophy

  Ilaria Andreana, et al., bioRxiv - Pathology 2024

  Quote: ... and DAPI (564907, BD Biosciences) and analyzed on a BD FACSCanto II apparatus.
• Single-cell transcriptomics reveals colonic immune perturbations during amyloid-β driven Alzheimer’s disease in mice

  Priya Makhijani, et al., bioRxiv - Immunology 2024

  Quote: ... Defrosted and PBS-re-hydrated tissues were subjected to FcReceptor blocking (BD Pharmingen, 553141) and primary antibody staining with rat-anti-mouse antibodies ...
• Single-cell transcriptomics reveals colonic immune perturbations during amyloid-β driven Alzheimer’s disease in mice

  Priya Makhijani, et al., bioRxiv - Immunology 2024

  Quote: ... KLRG1-BV480 (BD, Clone: 2F1), GL7-PerCpCy5.5 (Biolegend ...
• Generation and characterization of CRISPR-Cas9-Mediated XPC Gene Knockout in Human Skin Cells

  Ali Nasrallah, et al., bioRxiv - Genetics 2024

  Quote: ... samples were acquired using a FACSCanto™ II cytometer (BD Biosciences, Franklin Lakes, USA) and analyzed utilizing the online QOGNIT LEGENDplex™ program.
• Generation and characterization of CRISPR-Cas9-Mediated XPC Gene Knockout in Human Skin Cells

  Ali Nasrallah, et al., bioRxiv - Genetics 2024

  Quote: ... The analysis was carried out utilizing flow cytometry equipment (FACScan, BD LSRII flow cytometer, BD Biosciences). The post-analysis was done using flowing software (Turku Bioimaging ...
• Generation and characterization of CRISPR-Cas9-Mediated XPC Gene Knockout in Human Skin Cells

  Ali Nasrallah, et al., bioRxiv - Genetics 2024

  Quote: ... The analysis was carried out utilizing flow cytometry equipment (FACScan, BD LSRII flow cytometer, BD Biosciences). The post-analysis was done using flowing software (Turku Bioimaging ...
• Multi-omic QTL mapping in early developmental tissues reveals phenotypic and temporal complexity of regulatory variants underlying GWAS loci

  Timothy D. Arthur, et al., bioRxiv - Genomics 2024

  Quote: ... and Alexa Fluor® 647 Mouse anti IgG1 κ Isotype Clone MOPC-21 (BD Biosciences). Stained cells were washed three times ...
• Multi-omic QTL mapping in early developmental tissues reveals phenotypic and temporal complexity of regulatory variants underlying GWAS loci

  Timothy D. Arthur, et al., bioRxiv - Genomics 2024

  Quote: ... and immediately analyzed using FACS Canto II flow cytometer (BD Biosciences). The fraction of PDX1- and NKX6-1-positive cells were calculated using FlowJo software version 10.42.
• Multi-omic QTL mapping in early developmental tissues reveals phenotypic and temporal complexity of regulatory variants underlying GWAS loci

  Timothy D. Arthur, et al., bioRxiv - Genomics 2024

  Quote: ... Emerging iPSC colonies were manually picked after Day 21 and maintained on Matrigel (BD Corning) with mTeSR1 medium (Stem Cell Technologies) ...