Labshake search
Citations for Becton, Dickinson and Company :
1901 - 1950 of 1987 citations for 7 Benzothiazolecarboxylicacid 2 3 dihydro 2 thioxo methylester 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: Exponentially growing cells in six-well plates were treated with 3 μM BAY-876 or DMSO for 24h before cell cycle analysis using allophycocyanin (APC) BrdU Flow kit (BD Pharmingen). Briefly ...
-
bioRxiv - Immunology 2019Quote: ... Flow cytometry was typically performed on colonies constituting at least 20 cells as described previously.3 Cultured cells were analyzed with the BD Fortessa flow cytometers (BD Biosciences).
-
bioRxiv - Microbiology 2020Quote: ... The concentration of iron in LB was calculated using specifications provided in the “Bionutrients Technical Manual Vol 3” supplied by the manufacturer (BD Biosciences). For assays testing the role of iron availability ...
-
bioRxiv - Microbiology 2020Quote: ... NJ) were coated for 3□hours with 10□μg of Matrigel following the manufacturer’s instructions (Biocoat, BD Biosciences, San Jose, CA). Inserts were placed into 24-well dishes containing 500□μl of RPMI medium supplemented with 1% fetal calf serum ...
-
bioRxiv - Microbiology 2021Quote: Cells were harvested using trypsin and washed with PBS 3 times gently and then stained with propidium iodide (BD Bioscience, 556547) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... anti-mouseCD86-FITC (Clone: GL1) and anti-mouse CD40-FITC (Clone: 3/23) according to the manufacturer’s instructions (BD Bioscience, USA). All controls were performed using irrelevant isotype staining ...
-
bioRxiv - Immunology 2019Quote: ... Primary antibodies diluted as appropriate were: the rat anti-mouse MAC-3 antibody Clone M3/84 (1:100 dilution, BD Pharmingen), rabbit polyclonal NOS2 antibody (#ab15323 ...
-
bioRxiv - Developmental Biology 2019Quote: ... This strain was grown overnight from a frozen stock (25% glycerol) in 3 mL MRS medium (DifcoTM Lactobacilli MRS Broth, BD #288110) containing 10 µg/mL chloramphenicol ...
-
bioRxiv - Immunology 2020Quote: ... for 30 min prior to the addition of unconjugated anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences), brefeldin A (1 μl/ml ...
-
bioRxiv - Immunology 2020Quote: ... in the presence of unconjugated anti-CD28 (clone L293) and anti-CD49d (clone L25) (each at 3 μl/ml; BD Biosciences) and IL-2 (10 IU/ml ...
-
bioRxiv - Neuroscience 2021Quote: ... Purified bone marrow cells were centrifuged at 1500 rpm for 3 min and filtered through a 100 μm cell strainer (BD Biosciences). The bone marrow cells were plated onto a 100 mm culture dis h or six-well plate (BD Biosciences ...
-
bioRxiv - Pathology 2021Quote: ... 0.2 micron sterile filtered) using an insulin syringe (31-gauge, 6 mm length, 3/10 cc, BD Veo Ultra-Fine needle). Glucose and lactate levels were measured using a minimally invasive tail-prick method at designated timepoints post-injection.
-
bioRxiv - Immunology 2021Quote: ... NK cells purity at the end of the 3-week coculture was (> 90%) as determined by flow cytometry (BD Accuri C6) using CD56 (5.11H11 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were stained with cell phenotyping antibodies in a 1:1 volume ratio of 3% FBS and Brilliant Stain Buffer (BD Biosciences) according to standard procedures ...
-
bioRxiv - Immunology 2022Quote: Splenocytes from infected mice were incubated for 5 hours at 37°C 5%CO2 in cRPMI prior intra-cytoplasmic detection of active-caspase 3 (BD Bioscience) using BD Fixation/Permeabilization kit (BD Bioscience) ...
-
bioRxiv - Microbiology 2022Quote: ... in PBS at 37°C for 30 mins followed by one wash with PBS at 450 g for 3 mins and finally resuspend in 100 μl PBS for analysis using flow cytometry (BD Fortessa).
-
bioRxiv - Microbiology 2022Quote: ... Cells were harvested and washed with 3 x with PBS before performing FACS using a BD FACS Celesta Cell Analyzer (BD Biosciences).
-
bioRxiv - Physiology 2022Quote: ... Sections were washed 3 times in wash buffer then stained for Alexa Fluor 555 mouse anti-E-cadherin (BD, Cat. 560064) for 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... Confluent definitive endoderm cells are then passed 1:3 in presence of Rock Inhibitor on growth factors reduced Matrigel (BD Biosciences) and cultured for 8 days in differentiation medium (DMEM F12 ...
-
bioRxiv - Cell Biology 2023Quote: ... After 1 h of incubation at 37 °C the cells were washed 3 times with HBSS followed by trypsinization and signal was measured using FACScan and FACSCalibur systems (BD Biosciences). Data was analyzed with FlowJo™ v10 Software (BD Biosciences).
-
bioRxiv - Immunology 2023Quote: ... with 3×104 PFU or injected intracranially (10uL) with 50 PFU of LGTV-TP21 using insulin syringes (BD Medical, #BD-329461). At appropriate times post infection ...
-
bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend ...
-
bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
-
bioRxiv - Microbiology 2023Quote: ... cells were incubated with fluorochrome-conjugated monoclonal antibodies (mAbs) diluted at 1:200 into a 1:3 solution of Brilliant Staining Buffer (BD 563794): Cell Staining Buffer ...
-
bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells followed by an incubation with the SM/Chol probe OlyA (3 µM) and primary anti-IFN-γR1 antibody (1:50) (BD Biosciences) together in 1% BSA-PBS at room temperature for 90 min ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco, BD Bioscience, 15 g/L agar Bacto, BD Bioscience). Plates were left at room temperature until colonies were detectable and distinguishable (2-4 days) ...
-
bioRxiv - Immunology 2024Quote: ... and 5×104 of each were combined in 96-well U-bottom plates with anti-CD40 (100 ng/mL, clone HM40-3, BD Biosciences) in the presence or absence of OVA (10 μg/mL ...
-
bioRxiv - Systems Biology 2024Quote: ... freshly sorted lineage-labeled Dclk1+ cells were resuspended in LPM-3D medium (LPM-3) and mixed with growth factor-reduced Matrigel (BD Biosciences) in a 1:1 ratio ...
-
bioRxiv - Plant Biology 2024Quote: ... The dry volume (DV, m3) was measured by water displacement (Berta et al., 2010) and basic density (BD, kg m-3) calculated as reported by Carriero et al ...
-
IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... NJ) were coated for 3 hours with 10 μg of Matrigel following the manufacturer’s instructions (Biocoat, BD Biosciences, San Jose, CA). Inserts were placed into 24-well dishes containing 500 μl of RPMI medium supplemented with 1% fetal calf serum ...
-
bioRxiv - Cell Biology 2024Quote: Cells were seeded 24 h before treatment and were allowed to grow in the presence of 3 μg/mL BrdU (BD Pharmingen) for 48 h ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 µg of this genomic DNA from soluble fraction was mixed with 3 µg of anti-IdU/BrdU antibody (3D4 clone, BD Science) in BrdU-IP buffer composed of PBS and 0.0625% Triton X-100 overnight ...
-
bioRxiv - Cancer Biology 2024Quote: ... mice received a subcutaneous injection of 3 million VCaP cells in a mix of serum-free medium and 50% Matrigel (BD Biosciences). Once tumors grew to about 200 mm³ ...
-
bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and analyzed using FlowJo software (BD Biosciences). All antibodies are from Biolegend unless otherwise specified.
-
bioRxiv - Developmental Biology 2022Quote: Zebrafish wild-type and vsxKO retina sections from different developmental stages were analysed for the detection of apoptotic and mitotic cells using rabbit anti-active caspase-3 antibodies (BD Biosciences, 559565) and rabbit anti-phospho-histone H3 antibodies (Merck Millipore ...
-
bioRxiv - Immunology 2021Quote: ... cells were stained for surface markers (when required) then fixed/permeabilized by using PE Active Caspase-3 Apoptosis Kit (BD Bioscience, 550914) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: ... The cell pellet was then resuspended in 1 ml of T-R minimal medium and injected into the IsoCaRB system using a 3 ml syringe (BD Biosciences # 309657) and a 20-gauge needle (Sigma-Aldrich #Z192511).
-
bioRxiv - Cancer Biology 2020Quote: ... Mammary fat pad injections were performed in the 4th pre-cleared mammary fat pad of 3 weeks old female mice in 50% matrigel (BD Biosciences, Germany). Breast or prostate cancer-origin of xenografts were verified by a pathologist.
-
bioRxiv - Cancer Biology 2019Quote: ... Parental P18T and RAS pathway mutant knock-in patient-derived organoids were transplanted subcutaneous as single cells at a density of 3×10^5 organoids in 100 µl 50% Matrigel/medium with 10% collagen type I (BD Bio-sciences) mixture into NSG-B2m mice (JAX stock no ...
-
ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
-
bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
-
bioRxiv - Immunology 2022Quote: ... PBMC from the 3-time point from each patient were labelled using Single Cell Labelling with the BD Single-Cell Multiplexing Kit (BD Biosciences, #633781) and 52 different BD AbSeq Ab-Oligos reagents following the manufacturer’s protocol (BD Biosciences) ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were suspended in 500 μl PBS with 3 % FBS and then detected by flow cytometry (BD Biosciences, San Diego, CA, USA).
-
bioRxiv - Molecular Biology 2019Quote: Whole-mount retinas were blocked with horse serum 4°C overnight then incubated at 4°C for 3 days with primary antibodies: Mouse anti-Oct4 (1:100, BD bioscience, 611203), Rabbit anti-Sox2 (1:100 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Four times washing in 500 μl 1x PBS (1,150xg, 3 min) prepared the fixed cells for quantitative analysis by FACS (BD FACSCanto, BD Biosciences). The fluorescent MitoTracker dye was detected using the mCherry channel (excitation ...
-
bioRxiv - Neuroscience 2020Quote: COs were washed 3 times for 5 minutes in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...