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Citations for Becton, Dickinson and Company :
1851 - 1900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the cells expressing HaloTag were subjected to single-cell sorting into 96-well plates (FACSAria Fusion, BD Biosciences).
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bioRxiv - Immunology 2024Quote: NK cells were purified using NK Cell Isolation Kit (Miltenyi Biotech) or FACS melody™ (BD Biosciences, US) and were kept overnight in complete RPMI media with 50 ng/ml of recombinant human IL-2 (R&D Systems) ...
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bioRxiv - Systems Biology 2023Quote: ... Cells containing high levels of GFP or mCherry were sorted using the FACS Aria Fusion Cell Sorter (BD Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were trypsinized and plated at 1 million cells in a 10 cm2 culture dish (BD Falcon) containing αDFNK media containing a 1:1 ratio of Advanced DMEM (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with 1 µg/ml Doxycycline before sorting for GFP-positive cells (Aria 3 Sorter; BD). For lentiCRISPR v2 system ...
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bioRxiv - Biophysics 2024Quote: ... Panel for NK cells activation: cells were stained with anti-Human CD3-PE-Cy7 (clone SK7, BD Biosciences) and CD45-FITC (clone HI30 ...
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bioRxiv - Cell Biology 2024Quote: ... and 20,000 cells/sample were analysed by multi-channel flow cytometry on a LSRFortessa cell analyser (BD Biosciences). For FACS ...
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bioRxiv - Cancer Biology 2024Quote: ... the stained cells were subject to the cell cycle analysis by using a flow cytometer (FACSVerse; BD Biosciences), and data were analyzed with FlowJo software.
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bioRxiv - Plant Biology 2020Quote: 10-fold serial dilutions (OD600=1, 10-1, 10-2, 10-3) of yeast sample harbouring a DNA-binding domain (BD)-AtCIPK16 and either an activation domain (AD)-AtCBLs or an empty vector (AD ...
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bioRxiv - Bioengineering 2022Quote: ... Brooks Lifesciences) were coated with 200 μL of 2 mg/mL type 1 rat tail collagen (354249, Corning/BD Biosciences) diluted in DMEM equilibrated in 1/10 v/v of 7.5% w/v sodium bicarbonate solution (#25080-060 ...
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bioRxiv - Immunology 2021Quote: ... cells were either resuspended in 400μl of PBS 2%FCS 2mM EDTA and analyzed on Fortessa-X20/Symphony cytometers (BD Biosciences) or in RPMI media and used for cell sorting on a FACSAria II (BD ...
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bioRxiv - Immunology 2021Quote: ... Vα7.2+ cells were pre-incubated for 1 h and 30 min with LukED at 0.312 μg/mL before adding to the bacteria-pulsed THP-1 and co-culture at 2:1 ratio for 24 h in the presence of 1.25 μg/mL anti-CD28 mAb (L293, BD Biosciences). Monensin and brefeldin A (both from BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... whole blood was stained within 2 hours of blood draw for the surface markers CD45 (PerCP; DO58-1283; BD Biosciences), CD3 (FITC ...
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bioRxiv - Immunology 2021Quote: ... at 100ng/mL and IL-2 (100IU/mL) or expanded by plate-bound anti-CD3 mAb at 2μg/mL (BD Pharmingen) and soluble anti-CD28 mAb at 1μg/mL (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... and microglia (CD45med CD11b+were sorted into collection tubes coated with FCS and filled with complete RPMI 1640 medium (1% penicillin, streptomycin, 0.1% 2-ME) with 20% FCS using a FACSAria device (BD Biosciences). Purity of sorted populations was routinely above 95% ...
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bioRxiv - Immunology 2020Quote: ... for 2 h and 4 h respectively at 37°C in 5% CO2 and exposed to brefeldin A (BD Biosciences) as described in previous studies ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Bioengineering 2020Quote: ... The concentrations of cytokines IL-2 and IFN-γ were quantified using the corresponding ELISA kits (BD, 555190 and 555142).
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846; 1:200 mouse anti-Map2, BD Pharmagen ...
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bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: 100 μl of washed and number-adjusted yeast suspension were injected into each caterpillar’s distal right proleg with a 30G1/2” needle (BD Microlance) and a 1 ml NORM-JECT syringe ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were incubated on a cold block for 2-5 minutes before being plated in a 96 (12 x 8) grid on Omnitrays containing LB (BD, Franklin Lakes ...
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bioRxiv - Cell Biology 2022Quote: ... and Western blots (WBs): monoclonal Abs (mAbs) anti-flotillin-2 (610383; WB: 1/1000) and anti-GM130 (610823; IF: 1/500) from BD Biosciences ...
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bioRxiv - Developmental Biology 2022Quote: ... E.coli strain Rosetta™ 2(DE3)pLysS bearing pET21d-FGF10 was grown in 2x YT medium (BD Biosciences, Cat#244020) with Carbenicillin (50µg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Collagenase activity was quenched by dilution with FACS buffer (PBS, 0.5% FBS, 2 mM EDTA) and digestion products were filtered through 70 µm nylon filters (BD Biosciences). Cells were again washed with FACS buffer and immediately processed for immunostaining first by blocking Fc receptor binding with rat anti-mouse CD16/CD32 (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... Flow cytometry profiles of the HSPC compartment (Extended Data Fig.2, Fig.9) were analysed using FlowJo software (version 10.1, BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 2 ml of FACS buffer and fixed by adding 3.5 ml of fixation/permeabilization buffer (BD Biosciences) and rocking overnight at 4C ...
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bioRxiv - Pathology 2021Quote: ... Whole blood samples were then lysed and fixed in 2 ml of FACS Lysing Solution (BD Biosciences, San Jose, CA) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were first blocked for non-specific antigen binding (incubating for 20 min at RT with 2 µg/ml CD16/CD32 Rat anti-Mouse, unlabeled, Clone: 2.4G2, BD, 553142) then antibodies were added without washing off the blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... After staining for 45 min at 4°C cells were washed and taken up in 200 µl PBS+2%FBS and 1/1000 7AAD before being run on a LSR Fortessa (BD). Lineage out-put was analysed in FlowJo ...
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bioRxiv - Immunology 2022Quote: ... then fixed in 2% paraformaldehyde and stored at 4°C until acquisition on a FACSAria Fusion III flow cytometer (BD). Flow cytometry data was analysed with FlowJo version 9.9.6 (Tree Star).
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Biochemistry 2022Quote: ... washed and resuspended in FACS buffer (2% FBS in 1X PBS) and filtered through filter-topped flow tubes (BD falcon) using a luer-lock syringe at 2×106 cells/ml ...
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bioRxiv - Biochemistry 2019Quote: ... shRNA expression was induced by doxycycline (2 μg/ml) and sorted using a BD FACSAria™ III (BD Biosciences-US) cell sorter to select for the high TurboRFP expressing cells ...
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bioRxiv - Bioengineering 2019Quote: Blood was gently drawn from healthy volunteers who had not been subjected to any anticoagulant treatments for at least 2 weeks by standard venepuncture into sodium citrate vacutainers (2.7 mL, 3.2 % v/v sodium citrate; BD, NJ, USA). The informed consent was provided by each volunteer ...
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bioRxiv - Immunology 2019Quote: ... and activated with 2 μg/mL anti-mouse RP105 clone RP/14 (produced in house or BD Pharmingen Cat.# 562191).
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bioRxiv - Cell Biology 2019Quote: ... Samples were washed with 2 ml wash buffer and taken up in 0.5 ml wash buffer afterwards for FACS analysis (BD Fortessa).
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bioRxiv - Immunology 2021Quote: ... followed by FcR blockage with mouse serum (2%) and FcR blocking anti-mouse CD16/32 antibodies (clone 2.4G2, BD Biosciences). Next ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed twice in PBS with 0.5% BSA and 2 mM EDTA and run on LSR II flow cytometer (BD Biosciences). Data were analyzed using FlowJo software ...
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bioRxiv - Bioengineering 2021Quote: ... All transformants were checked for the petite phenotype by growth on YEP-glycerol (1% w/v Bacto-yeast extract-BD Biosciences 212750, 2% w/v Bacto-peptone-BD Biosciences 211677 ...
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bioRxiv - Immunology 2021Quote: ... tibias and pelvis were harvested by crushing in PBS containing 2% of FBS and erythrocytes were lysed using a hypotonic buffer from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and IL-2 concentration were measured on non-diluted or 10-fold diluted supernatants using Human cytometric assay from BD according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... the invading cancer cells were fixed with 100% cold methanol and stained with 0.05 mg/mL 4,6-diamidino-2-phenylindole (DAPI; BD Biosciences, 564907). The invading cells in each chamber were counted under a fluorescence microscope ...
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bioRxiv - Cell Biology 2021Quote: ... and the concentration of IL-2 in culture supernatants was determined by ELISA according to the manufacturer’s instructions (BD Biosciences).
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bioRxiv - Genetics 2021Quote: ... in 200 μl of 2% FBS/PBS (v:v) and calculated using DIVA before sorting on a FACSAria fusion III cytometer (BD Biosciences). In order to maintain the ratios of MuSCs ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5mM EDTA) containing the indicated fluorophor-conjugated antibodies (CD138-APC [281-2] and B220-PE [RA3-6B2] purchased from BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... were routinely grown at 37 °C under anaerobic conditions in a Bactron III-2 anaerobic chamber (Shellab, USA) in BHIS medium: 3.7% weight vol−1 brain heart infusion broth (BD, USA) supplemented with 0.5% weight vol−1 yeast extract (BD ...
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bioRxiv - Immunology 2019Quote: ... and stained for membrane extracellular markers in Staining Buffer (PBS FBS 2% EDTA 5mM) and for intracellular markers using Cytofix/Cytoperm buffer set following manufacturer’s protocol (BD Biosciences). Acquisition of cytometric parameters was performed on an LSRII (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: One hundred microliters of whole blood from each of the cynomolgus macaques was stained with combinations of fluorescence-conjugated monoclonal antibodies: anti-CD3 (clone SP34-2, Alexa700; BD), anti-CD4 (clone L200 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by fixation in 2% PFA in PBS before subjecting them to flow cytometry using the LSR-II (BD Biosciences). Data was analyzed by FlowJo v10 and graphed by Prism 8 (GraphPad).