Labshake search
Citations for Becton, Dickinson and Company :
1851 - 1900 of 8130 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... between the NdeI or NcoI and BamHI restriction sites in order to express N-terminal fusion proteins with the GAL4 DNA binding domain (BD) or with the GAL4 activation domain (AD) ...
-
bioRxiv - Immunology 2021Quote: ... in the last 5 hours of culture a protein transport inhibitor Brefeldin A (1µL/mL) was added (BD Biosciences, USA). Cells were fixed using the BD Cytofix/Cytoperm® kit (BD Biosciences ...
-
bioRxiv - Plant Biology 2019Quote: ... The coding sequence of AGL16 was cloned into pAD-GAL4-2.1 (AD vector) and the putative protein binding sites were cloned into pHIS2 (BD vector). These constructs of pAD and pHIS2 plasmids were introduced into Y187 yeast strain by heat shock ...
-
bioRxiv - Biochemistry 2020Quote: ... this threshold is likely a consequence of the fact that the target protein in all six ternary complex crystal structures is a bromodomain (BD), of either Brd4 ...
-
bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
-
bioRxiv - Immunology 2020Quote: ... The levels of IFNg protein expression was determined by intracellular staining after exposure to 4 hours of GolgiStop and GolgiPlug (BD).
-
bioRxiv - Immunology 2020Quote: ... IFN-γ and IL-4 protein in culture supernatants were quantified using the Cytokine Bead Array according to the manufacturer’s instructions (BD Biosciences)
-
bioRxiv - Developmental Biology 2024Quote: ... Membranes containing the transferred proteins were rinsed and blocked in 0.1% Tween-20 in Tris-buffered saline (TBST) containing 5% nonfat milk (BD Biosciences) at RT for 1 h ...
-
bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
-
bioRxiv - Biochemistry 2024Quote: ... AtHSP90-3 and ASDURF were transferred to the pGADT7-GW and pGBKT7-GW destination vectors using Gateway LR Clonase II enzyme mix to produce fusion proteins of the Gal4-activation domain (AD) and GAL4 DNA-binding domain (BD), respectively ...
-
bioRxiv - Immunology 2021Quote: All flow cytometry assays were set to acquire 10,000 events excluding debris and doublets using a FACSVerse™ flow cytometer (BD Biosciences, San Diego, CA, USA) and FlowJo v10.6 software (Ashland ...
-
bioRxiv - Cancer Biology 2021Quote: ... were collected from transwell assay and the mean fluorescence intensity of the green signal (VC3AI) was then determined by FACS Calibur flow cytometry (BD Biosciences, San Jose, CA, USA). Control cells were treated with 1 µM Actinomycin D as a positive control for cell death.
-
bioRxiv - Immunology 2021Quote: ... IgA and IgM antibodies were quantified in the CVL specimens using the human immunoglobulin cytokine bead array (CBA) flex set assay (BD Biosciences, San Jose, CA, USA). The Ig CBA assay was performed as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... CD4 T cell counts were also enumerated at all study visits using the 4-colour MultiTEST/Trucount assay (Becton Dickinson, San Jose, CA, USA) and analyzed further by flow cytometry on a FACSCalibur (BD Biosciences, San Jose, CA, USA). The median treatment-free follow-up time for the patients was 376 days [IQR ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The ELAM9 RAW264.7 cells used in the NF-κB activation assay were analysed using a BD FACS Canto II flow cytometer from Becton, Dickinson and Company (NSW, Australia), equipped with a high throughput fluorescence-activated cell sorting (FACS ...
-
bioRxiv - Synthetic Biology 2022Quote: ... all samples were mixed 1:1 with prewarmed CytoFix Fixation Buffer (BDBiosciences #554655) and incubated at room temperature for 15 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... rabbit anti-MCM2 (Cell Signaling, 4007S; 1:1000; BD Biosciences, 610701; 1:1000), rabbit anti-MCM3 (Cell Signaling ...
-
bioRxiv - Developmental Biology 2019Quote: ... Figure 1 and figure 2: CD235a-PeCy7 (1:100, BD, GA-R2(HRI2)) ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-Pdcd6ip (AIP-1/Alix) (BD transduction lab 611620, 1:500); goat polyclonal anti-TSG101 (Santa Cruz sc-6037 ...
-
bioRxiv - Microbiology 2020Quote: ... 250 µg mL−1 L-cycloserine and 1.5% weight vol−1 (BD, USA) (TCCFA plates) ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse anti-nestin (1:200 in vivo, 1:500 in vitro, BD Bioscience), chicken anti-vimentin (1:10,000 ...
-
bioRxiv - Cancer Biology 2022Quote: CD44 (1:200, IM7, #560568), CD11b (1:800, M1/70, #563553) (BD Biosciences); MHC-I (1:100 ...
-
bioRxiv - Physiology 2022Quote: ... Gr-1 (catalog no. 550291, 1/50, BD Pharmingen, Franklin Lakes, NJ, USA) and the cholangiocyte marker cytokeratin-19 CK19 (TROMA-III 1/500 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Col IΔ R/R fibroblasts diluted 1:1 with BD Matrigel (BD Biosciences) in a total volume of 100 μl ...
-
bioRxiv - Cancer Biology 2019Quote: ... A 100 μl volume of 1:1 mixture of Matrigel (354248; BD Biosciences) and 1 × 106 MiaPaCa2 cells alone ...
-
bioRxiv - Immunology 2021Quote: ... anti-CD101 (clone Moushi101, 1:100 eBioscience; clone 307707, 1:100 BD Biosciences). For MMP-9 and IL-1ß intracellular staining ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal antibodies to Aurora B (AIM-1; BD Bioscience 611082, 1:100) and H3S10ph (6G3 ...
-
bioRxiv - Cancer Biology 2022Quote: ... or RIVA cells were mixed in a 1:1 in Matrigel (BD Biosciences)/PBS and injected into the flanks of 10-week-old NSG mice ...
-
bioRxiv - Bioengineering 2024Quote: ... and anti-E-cadherin-1 (ECAD; 1:500; 610181, BD Biosciences, CA, USA). Nuclear counterstaining was performed with DAPI (Nacalai Tesque ...
-
bioRxiv - Genomics 2023Quote: ... and incubated overnight with Purified Mouse Anti-TEF-1 (1:500, BD-610922) or mouse anti-YAP1 H-9 (1:150 ...
-
bioRxiv - Cancer Biology 2021Quote: Cell cycle analysis was performed using the BrdU Flow Kit according to the manufacturer protocol (BD, FITC BrdU Flow Kit; Cat. No. 559619), with cells pulsed with BrdU for 1 hour at 37°C ...
-
bioRxiv - Bioengineering 2024Quote: pEU-E01 cell-free wheat germ expression vector containing ampicillin resistance and cell-free wheat germ expression kit (Cat# CFS-CPLE-BD Proteoliposome BD Kit) were obtained from CellFree Sciences Co. ...
-
bioRxiv - Immunology 2019Quote: ... were injected subcutaneously along with engineered stromal cells (1:1 to 1:5 ratio HSPC/MS5) in 200 μl of ice-cold Matrigel® (BD Biosciences). Mice were sacrificed at day 12 of differentiation by cervical dislocation and Matrigel® plugs were collected ...
-
bioRxiv - Immunology 2021Quote: ... and re-suspended in 100 μL permeabilisation buffer (FACS buffer, 1% BSA, 1 mM EDTA in PBS containing 1 X BD permeabilisation reagent). FITC-conjugated anti-influenza NP monoclonal antibody (Abcam clone 20921 ...
-
bioRxiv - Neuroscience 2019Quote: ... Primary antibodies (Claudin-5, Abcam Ab131259, 1:1000; Collagen IV, Abcam Ab6586, 1:500; Hemoglobin, R&D Systems G-134-C, 1:300; P62, BD Biosciences 610833 ...
-
bioRxiv - Cell Biology 2022Quote: ... F4/80 (1:100 dilution, clone Cl:A3-1; Serotec, Cat# MCA497) and CD11b (1:100 dilution, clone M1/70; BD Biosciences, Cat# 550282).
-
bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100, Anti-GM130 BD Transduction 610823) for 1 hour and 30 minutes at room temperature in blocking/permeabilization solution ...
-
bioRxiv - Immunology 2024Quote: ... labelled K562 at a ratio of 1:1 for 1 hour and conjugate formation was evaluated by flow cytometry (Fortessa X20™, BD Biosciences).
-
bioRxiv - Cancer Biology 2023Quote: ... SCC13 tumour cells and fibroblasts were mixed in a 1:1 ratio in complete DMEM containing 1% Matrigel (BD Biosciences, Cat# 354230). Cells were plated on 8-well glass bottom culture slides (Corning ...
-
bioRxiv - Immunology 2023Quote: ... FITC CD8 (Clone 53-6.7, 1:25), Alexa Fluor 700 CD44 (Clone IM7, 1:50), and PE CD62L (Clone MEL-14, 1:50) (BD Pharmingen, CA), and the yellow LIVE/DEAD® dye (1:300) ...
-
bioRxiv - Immunology 2019Quote: ... Tissue homogenates and blood were serially diluted in PBS and bacteria enumerated by standard plate counting on tryptic soy agar (TSA; Becton, Dickinson and Company, Sparks, MD). Agar plates were incubated at 37°C overnight.
-
bioRxiv - Synthetic Biology 2020Quote: ... Bacteria were cultured at room temperature (~25°C) in BBL Trypticase Soy Broth (TSB) or on Trypticase Soy Agar (TSA) plates (Becton, Dickinson and Company, MD, USA). For liquid cultures ...
-
bioRxiv - Immunology 2022Quote: ... the total number of cells on the bottom of the plate were stained and counted using the FACSCelesta flow cytometer (BD Biosciences, Franklin Lakes NJ, USA) with plate-acquisition mode and defined volumes.
-
bioRxiv - Immunology 2023Quote: ... The total number of cells on the bottom of the plate was stained and counted using the FACSCelesta flow cytometer (BD Biosciences, Franklin Lakes NJ, USA) with plate-acquisition mode and defined volumes.
-
bioRxiv - Cancer Biology 2024Quote: ... single GFP-expressing cells were sorted in 96-well plates by a fluorescence-activated cell sorter (FACS) FACSAria II cell sorter (BD Biosciences, San Jose, CA, USA). Two weeks later ...
-
bioRxiv - Immunology 2023Quote: ... 5.0 × 104 CD4+ T cells were serially diluted onto either α-IL-2 or α-IFNɣ sterile coated PVDF 96 well plates (BD Biosciences, NJ, USA). The CD4+ T cells were then activated for 24 or 72 h in complete DMEM ...
-
bioRxiv - Cancer Biology 2023Quote: ... The final pellet containing organoids was re-suspended in Matrigel and distributed in 96-well culture plates containing an underlay Matrigel layer (Growth Factor Reduced Matrigel, BD Biosciences, San Jose, CA, USA). Cultures were maintained at 37 °C in serum-free basal medium (DMEF/F12 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... We estimated infection prevalence (percent of RPF+ infected spores in a sample of 100,000) by running a sample of spores from each plate on a BD FACSCalibur flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ). FCS files were imported into FlowJo v10.8.1 for analysis ...
-
bioRxiv - Microbiology 2023Quote: ... CLASP11256−1538- and CD2AP-TurboID constructs were sorted into a 96-well plate as single cells with the FACS sorter Aria III (BD Biosciences, San Jose, California, USA).
-
bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. Surface expression level of S proteins (Extended Data Fig. 6a) was measured using FACS Canto II (BD Biosciences) and the data were analysed using FlowJo software v10.7.1 (BD Biosciences) ...