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Citations for Becton, Dickinson and Company :
1851 - 1900 of 6794 citations for 6 4 methylpiperazin 1 yl pyridine 3 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 20 μm paraformaldehyde fixed lung sections from Mtb-mCherry infected I-Tomcat Ai6 and Sp140−/− I-Tomcat Ai6 mice were stained at 4°C overnight with BV421-labeled SIRP□ (P84, BD Biosciences), Pacific Blue–labeled B220 (RA3-6B2 ...
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bioRxiv - Biochemistry 2022Quote: ... Salmonella from infected macrophages was fixed for 15 min in 4% PFA/PBS before analysis on a flow cytometer (Symphony, BD Biosciences) using thresholds on side scatter (SSC ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometry evaluation was performed the following day using a 4-laser (405 nm, 488 nm, 561 nm, 640 nm) LSRFortessa analyzer (BD instruments). The following antibodies and reagents were used for flow cytometry experiments ...
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bioRxiv - Immunology 2022Quote: ... Cells were then resuspended in FACS buffer (1% BSA in D-PBS) and kept at 4°C in dark until analyzed with a customized BD LSR Fortessa flow cytometer (BD Biosciences). Compensation was performed using ArC and UltraComp compensation beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood chimerism was determined in recipients by blood collection via cheek bleeds every 4 weeks for 16 weeks and cells were analyzed by flow cytometry using the LSRII (BD Biosciences). Long-term multi-lineage reconstitution (LTMR ...
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bioRxiv - Pathology 2023Quote: ... then incubated for 20 minutes at 4°C with a combination of the following mouse anti-human antibodies: AF488 anti-CD3 (clone UCHT1, BD Biosciences), PE anti-CD203c (clone NP4D6 ...
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bioRxiv - Cell Biology 2023Quote: ... and cells fixed in 4% paraformaldehyde (PFA) were analyzed on a Stratedigm (S1000EXI) cytometer with post- processing using FlowJo software (BD Biosciences). For all other analyses ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Immunology 2023Quote: T cells were staining 30 min at 4°C with anti-human CD3-PE (Cyto Matin Gene, CMGCD3-P100) anti-CD3-PerCP (BD, 347344), anti-CD3-FITC (BD ...
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bioRxiv - Immunology 2023Quote: ... for 20 mins at RT and then fixed samples were then resuspended in 100µL wash buffer prior to acquisition on the 4-laser BD® LSRII (BD Biosciences). Instrument setup and calibration were performed using EuroFlow standard operation procedures as described previously.43
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for 15 min at 4 °C with a purified mouse anti-rat CD32 FcγII (clone D34-485, Rat BD Fc Block) to block unspecific binding ...
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bioRxiv - Developmental Biology 2023Quote: MACS depleted non-hematopoietic cells were stained with the antibodies listed in Table S1 for 20 min at 4°C and analyzed on a spectral cytometer ID7000 (SONY) as described(4) or sorted with a BD FACS Aria III (BD Biosciences) according to the guidelines for the use of flow cytometry and cell sorting(5).
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bioRxiv - Developmental Biology 2023Quote: Hematopoietic cells were stained with the antibodies listed in Supp Table S1 for 20-30 min at 4°C and analyzed on a custom BD LSR Fortessa (BD Biosciences). For sorting ...
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bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... They were finally washed with 4 mL FACS buffer and re-suspended in 0.5 mL to be analyzed in a LSR Fortessa X20 (BD Biosciences, Europe). For live staining ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then fixed (eBioscience #00-8222-49) at 4 °C for 20 minutes and permeabilization (eBioscience #00-8333-56) before staining for intracellular IFNγ (BV650, BD #563854) and TNFα (BV421 ...
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bioRxiv - Microbiology 2023Quote: Stained cells were stored for up to 2 days at 4°C in the dark (TRAIL-R1) and flow cytometry was performed on a BD FACSCanto II (BD Biosciences). Raw data were exported to FlowJo v10.8.1 for analysis and plotting.
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then fixed in 2% formaldehyde and stored at 4°C until flow cytometry on an LSR II flow cytometer (BD Biosciences). Cell surface markers included CD3 (clone 17A2 ...
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bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
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bioRxiv - Immunology 2023Quote: ... T cells isolated from lymph nodes or tumors were mixed with 3×106 congenically marked B6 splenocytes and incubated with 1 μg/mL of OVA peptide and/or 2 μg/mL of GP peptide for 4-5h at 37°C in the presence of GolgiPlug (BD Biosciences). After staining for cell surface molecules ...
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bioRxiv - Microbiology 2023Quote: Ferrets were sedated with 4% isoflurane and blood was collected via the jugular vein into a heparinized collection tube (BD 366643). Plasma was separated via centrifugation and stored at –80°C until use ...
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bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were incubated overnight at 4°C in the dark and sample analysis performed the following day using an LSR FortessaTM X-20 device (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... according to manufacturer instructions and stored at 4°C protected from light before acquisition on a FACSCalibur cytometer equipped with CellQuest Pro software (BD Biosciences) within 1 week of fixation ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... The tube was then stored in the dark at 4°C for up to 2 hours prior to acquisition on a LSRII or FACSLyric analyzer (BD Biosciences).
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bioRxiv - Molecular Biology 2023Quote: ... the cells were fixed in 4% PFA for 15 minutes at room temperature and permeabilised in Perm III buffer (BD Bioscience) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Immunology 2024Quote: Live single CD45+TCRβ+CD4+IL10+ cells were sorted from isolated lamina propria cell suspension from three pooled Giardia-infected mice per sample (total of 4 samples) using a BD FACSAria Cell Sorter (BD Biosciences). Then ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with staining buffer, fixed, and permeabilized (20 min, 4°C, dark) using Fixation and Permeabilization Solution (BD Biosciences). Cells were then stained with S100A9 (Clone ...
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bioRxiv - Neuroscience 2023Quote: ... Immunopurified Ngfrp75-positive mouse OECs were plated on PLL-coated 4-chamber polystrene-vessel culture slides (BD Falcon, San Jose, CA) or used for western blots ...
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bioRxiv - Neuroscience 2023Quote: ... cell suspension was incubated with Fc block antibodies for 5 minutes at 4 °C and then labeled with anti-mouse Thy1.2 magnetic particles (BD IMag™) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... surface antibodies were incubated with cells for 30 minutes at 4°C in hFACS buffer and Brilliant Stain Buffer (BD Biosciences). For intracellular staining ...
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bioRxiv - Physiology 2024Quote: ... Small intestine lamina propria cells from male C57BL/6J mice were prepared and stained with surface molecules for 30 min at 4 followed by sorting on a FACS Aria III cell sorter (BD Biosciences). A gating strategy of live Lineage−CD127+KLRG1-c-Kit+ was used to sort the ILC3s.
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bioRxiv - Cell Biology 2023Quote: ... 0.25% Triton PBS before incubation overnight at 4°C with the following antibodies: Rat monoclonal to mouse Sca1 (740450, BD Biosciences), Rabbit monoclonal to mouse SOX9 (ab185230 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with the specific primary antibodies (1:300 CD24-PerCP-Cy5.5, BD 562360; 1:100 CD31-BUV395, BD 740239; 1:200 CD45-BUV395, BD 565967 ...
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bioRxiv - Neuroscience 2020Quote: ... CD8a APC-R700 (1:150 or 1:200, clone 53-6.7), CD69 BV421 (1:100, clone H1.2F3), CD25 BB515 (1:150, clone PC61) (BD Biosciences); CD4 FITC (1:500 ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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bioRxiv - Bioengineering 2023Quote: ... pneumoniae serotype 3 (Sp3) (ATCC; Manassas, VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... BD Biosciences], 1:150 anti-CD226/DNAM-1-BV510 [clone DX11, BD Biosciences], 1:400 anti-CD3-AF700 [clone UCHT1, BD Biosciences] ...
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bioRxiv - Immunology 2021Quote: ... no stimulation and a peptide pool spanning the spike protein at a concentration of 1 μg ml−1 of each peptide in the presence of 1 μg ml−1 of anti-CD28 (clone CD28.2, BD Biosciences) and anti-CD49d (clone 9F10 ...
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bioRxiv - Neuroscience 2019Quote: ... 1:200 Biolegend, #141723; CD45-BV786, 1:200, BD Biosciences #564225; CX3CR1-FITC, 1:100, Biolegend, #149019; CD11a, 1:20, BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... mouse primary at 1/100, Dako; CldU: rat primary at 1/1,000, Accurate Chemical; IdU: mouse primary at 1/1,000, BD Biosciences) and with a rabbit anti GFP (Millipore ...
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bioRxiv - Cell Biology 2019Quote: ... targeting the extracellular/luminal domain, 555538, 1:500) and mouse anti-EEA1 (610456, 1:200 for immunofluorescence; 1:1000 for western blot) from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... ratio of 10:1 (PBMC) or 1:1 (purified NK cells) in the presence of GolgiStop (0.7 μl/ml, BD Biosciences), Brefeldin-A (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... Samples were next stained with surface marker antibodies (CD45-AF700 1:500 103128 Biolegend, CD31-Pacific Blue 1:500 102421 Biolegend, TER119-BUV496 1:500 741079 BD, PDGFRα-APC 1:500 135907 Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 1:100) from eBioscience, and CD45 (564279, 1:200), IL-10 (566295, 1:100) and TCRγδ (563532, 1:100) from BD Bioscience.