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Citations for Becton, Dickinson and Company :
1801 - 1850 of 9716 citations for Anti p46 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... IdU and CldU were then detected with the following primary antibodies in blocking solution for 1 hour at room temperature: mouse anti-BrdU (B44) (1:25) (BD Biosciences cat#347580) for IdU and rat anti-BrdU [Bu1/75 (ICR1)] (1:25 ...
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bioRxiv - Physiology 2021Quote: ... Following resuspension in PBS and passage through a 70μm strainer, cells were incubated in Fc block solution (Bio Rad, Cat# BUF041A) and PerCP-conjugated anti-CD11b antibody (BD Pharminogen, Cat# 550993). After PBS washes ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Immunology 2021Quote: NK cells and cancer cells were cocultured at 10:1 ratio and supplemented with 20ul of PE mouse anti-human CD107a antibody (BD Pharmingen™, #555801) in a total volume of 220ul in a 96 well plate at 37C incubator for 90 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 50 µL Perm/Wash buffer containing a cocktail of anti-human antibodies including IFNγ BV480 (BD, 566100, clone B27), TNFα BV605 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: T-cell degranulation was evaluated by adding an anti-CD107a antibody at the beginning of coculture (CD107a-FITC clone H4A3, BD, Catalog N° 555800) and Golgi Stop (BD ...
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bioRxiv - Immunology 2022Quote: ... the individual antibodies used for staining were mixed with one drop of negative control beads and anti-mouse IgG beads (BD Biosciences, CA, USA). For the dead cell stain ...
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bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
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bioRxiv - Microbiology 2022Quote: ... was used as primary antibody for samples and mouse anti-clathrin heavy chain clone 23 (1:2,000; BD Biosciences, Franklin Lakes, NJ, USA) for the detection of clathrin heavy chain as a loading control ...
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bioRxiv - Neuroscience 2022Quote: ... Following overnight incubation the plate was washed 3 times with DPBS and then incubated with 20 µl anti-CD45 antibody (BD Pharmingen, catalog #550539) in 12 ml of 0.2% BSA for a minimum of 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Samples are ultracentrifuged and pellets were resuspended and incubated onto 96 well ELISA plates containing 5ug/mL anti-syn42 monoclonal antibody (BD transduction #610-787). ELISAs were carried out on test samples run alongside serial diluted standard synthetic α-Syn ...
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bioRxiv - Immunology 2024Quote: ... The labeled cells were cultured in complete RPMI-1640 medium plus IL-2 (20 units/ml) or IL-2 and anti-CD3 antibody (1μg/ml) (BD Pharmingen (San Diego, CA), or alternatively plus IL-4 (4ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5-μm sections were stained with the following antibodies following citrate antigen retrieval and blocking with 10% normal donkey serum: mouse anti-glutamine synthetase (BD Bioscience, 1:2000), mouse anti-Ki67 (BD ...
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bioRxiv - Developmental Biology 2023Quote: ... pre-incubated with anti-mouse CD16/CD32 (FcRII block) and then stained with the following anti-mouse monoclonal antibodies: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Immunology 2023Quote: ... pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL, clone: 145-2C11, BD Biosciences, San Diego, CA, USA) and supplemented with soluble anti-CD28 antibodies (2ug/mL clone ...
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bioRxiv - Immunology 2023Quote: ... and the cells were stained with antibodies specific for the following proteins: CD4 (PE Rat Anti-Mouse CD4; Clone RM4-5; BD Biosciences; Cat # 553049), H-2Dd (Anti-mouse H-2Dd antibody ...
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bioRxiv - Microbiology 2023Quote: ... we stained cells expressing wild-type and mutant SFV glycoproteins with a mouse anti-SFV immune ascitic fluid (ATCC Cat#: VR-1247AF) or a control mouse IgG2b antibody (BD Biosciences Cat#: 557351). Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat# ...
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bioRxiv - Physiology 2024Quote: ... then cells were washed with 1x PBS and resuspended in FACS buffer and non-specific binding to Fc receptors was blocked by incubation with anti-CD16/32 antibody (BD Pharmingen, Cat# 553141) and the FACS antibodies BV711 conjugated anti-CD11b and Pacific blue conjugated anti-CD45 antibodies (Cat# 101241 ...
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bioRxiv - Microbiology 2024Quote: ... The homogenized cell suspension was washed and resuspended in FACS buffer containing the PE-conjugated Rat anti-mouse F4/80 antibody (BD Horizon, Cat-565411) or eFluor450-conjugated anti-mouse CD11c antibody (eBioscience) ...
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bioRxiv - Neuroscience 2024Quote: ... The cover slips were then permeabilized with 0.3% Triton X-100 and sequentially stained with rat anti-CD44 antibody (BD Biosciences, 550538; 1:200), and corresponding Alexa 488-conjugated secondary antibodies (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... The following antibodies were used (all antibodies from BD Biosciences, unless stated otherwise): CD45 APC (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-CD63 and anti-GM130 (BD biosciences cat ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... Synaptic ribbons were labeled with anti-CtBP2 (1/200 Goat polyclonal, Santa Cruz, USA ; cat # SC-5966 or 1/200 Mouse IgG1 monoclonal antibody BD Biosciences, cat # 612044). Otoferlin was labeled with a mouse monoclonal antibody (1/200 AbCam ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...
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bioRxiv - Cancer Biology 2022Quote: qNSCs and aNSCs from the SVZ were FACS purified from GFAP::GFP mice as previously described (Codega et al., 2014) using the following antibodies: anti-mCD24 PE (1:2000; BD Pharmingen 12-0242-82), biotin conjugated anti-mCD133 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Negative cells were sorted for the absence of PD-L1 by staining with a PE-labeled anti-mouse PD-L1 antibody (clone MIH5, BD Biosciences, Franklin Lakes, NJ) using a FACSAria III (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were FACS sorted four times for expression of CD40L using an anti-CD40L antibody (clone TRAP1, BD Bioscience, San Jose, California). This resulted in a stable CD40L-expressing cell line that was cultivated in G418 containing selection media to maintain expression ...
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Genomics 2022Quote: The collected cells were first stained with live/dead aqua to exclude non-viable cells from the analysis and stained with fluorophore-conjugated antibodies against surface markers anti-CD45-BV786 (BD Bioscience Clone: OX-1), anti-CD11b-V450(BD Bioscience Clone ...
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bioRxiv - Cell Biology 2024Quote: ... upper membrane parts were first probed with rat monoclonal anti-pSMAD1/9 antibody (pS463/pS465, pS465/pS467, 1:1,000, #562508, BD Biosciences, East Rutherford, NJ, USA), or rabbit monoclonal anti-pSMAD2 (S465/467 ...
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bioRxiv - Developmental Biology 2024Quote: ... Animal caps were blocked for 1 hour using sheep serum and bovine serum albumin and then incubated in 1:250 anti-β-catenin antibody (BD Bioscience cat# 610154) in blocking solution overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Genetics 2023Quote: ... USA)/anti-rabbit IgG Alexa 488 (Jackson Immuno Research lab, West Grove, PA, USA) and 10µg/mL biotinylated anti-CD45 antibodies (BD Bioscience, Franklin Lakes, NJ, USA)/anti Rat IgG Alexa Plus 647 (Thermo Fisher Scientific ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: pCAF sorting based on TNFRSF12A protein level: 106 cells were incubated with BD Horizon BV421 anti-TNFRSF12A antibody (1/140, 565712 BD Biosciences, Franklin Lakes, NJ) and human TruStain FcXTM (1/200 ...
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bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
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bioRxiv - Cancer Biology 2023Quote: Ba/F3 cells expressing human MPL protein that were either cultured normally or treated with drugs were harvested and stained for surface MPL using PE-conjugated anti-human MPL antibody (BD Pharmingen 562159,1:50 dilution) in staining buffer for 30 min at 4°C.
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bioRxiv - Microbiology 2023Quote: ... The cells were washed with PBS+FBS and incubated with the monoclonal antibody anti-mouse CD8 T conjugated to Fluorescein Isothiocyanate (FITC) (BD-Pharmingen, San Diego, CA), which was suitable for determining the phenotype of CD8+ T cells ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight in 0.1M PBS + 0.1% TX-100 solution containing primary antibody for rat anti-CD31 (label endothelial cells; 1:200 dilution, BD Clone MEC 13:3: #553370) or sheep anti-CD93 (label endothelial tip cells ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin in PBS for 45 min at 37°C was followed by incubation with a primary antibody mix of anti-BrdU detecting IdU (mouse, #347580, BD, Franklin Lakes, NJ, USA) and anti-BrdU detecting CldU (rat ...
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bioRxiv - Immunology 2024Quote: ... Lymphocytes were cultured in RPMI supplemented with 10% FBS and 50 μM beta-mercaptoethanol (RPMI media) Lymphoblasts were generated by activation with plate bound murine anti-CD3ε antibody (BD Biosciences, Clone: 145-2C11) in the presence of 100 U/ml of human IL-2 and soluble anti-CD28 antibody (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed with phosphate buffer three times for 10 minutes each and incubated with primary antibody (rat anti-mouse CD31, BD Biosciences, 550274, 1:100) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... according to the protocol from vendor and stained with intracellular cytokine specific antibodies Rat anti-mouse Rorγt- Brilliant Violet-650 (BD Biosciences, #564722, clone Q31-378), mouse anti-GATA3 Alexa Fluor F488 (BD Bioscience #560163 ...
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bioRxiv - Immunology 2024Quote: ... was prepared by coating the well with 50 μl of 10 μg/ml of anti-mouse IFNγ monoclonal capture antibody (BD Biosciences; cat. no. 551309) in PBS and allowed to incubate at 4°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... The ELISPOT plate was washed five times with PBST and 50 μl of 1 μg/ml of the anti-mouse IFNγ monoclonal detection antibody conjugated to biotin (BD Biosciences; cat. no. 551506) in PBS was added and allowed to incubate at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... α7 integrin-PE antibody (AbLab, Vancouver, Canada), CD9-APC antibody (eBioscience, San Diego, CA) and CD47-BV605 antibody (BD Biosciences, San Jose, CA) for 30 minutes on ice and washed ...