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Citations for Becton, Dickinson and Company :
1801 - 1850 of 2187 citations for 5 TERT BUTYL 2 PROPOXY BENZENESULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Polyclonal goat anti-mouse Ig conjugated to APC was used as a secondary antibody (2 μg/mL, BD Pharmigen #550826). For all antibodies ...
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bioRxiv - Microbiology 2023Quote: ... FITC-A between 2×104–106) or an LSR-Fortessa flow cytometer (BD Biosciences; for Fig 5A and Fig S5C) and data was plotted in FlowJo (ver ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H ...
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bioRxiv - Cancer Biology 2023Quote: Spleens and livers were harvested into ice cold FACS buffer (PBS supplemented with 2% FCS v/v) and then emulsified through a 70μM filter (BD Biosciences), centrifuged and washed in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/mL of anti-mouse IgE (553413, clone R35-72, BD Pharmingen) in 0.1 M sodium carbonate buffer pH 9.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lifted by pipetting in FACS buffer (D-PBS, 2% FBS, 0.1% sodium azide) and analyzed on a FACSCelesta (BD Biosciences). 20,000 events were collected per sample on the cytometer ...
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bioRxiv - Immunology 2023Quote: ... Staining was quenched by washing in FACS buffer (PBS with 2 mM EDTA and 2% FCS) after which cells were incubated with anti-CD16/32 (Fc block, clone 2.4G2, BD Biosciences) for 15 minutes at 4°C to prevent non-specific binding ...
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bioRxiv - Developmental Biology 2023Quote: ... one million cells were transferred in 100 μl of FACS buffer supplemented with 0.5μM Y27632 and then added 2 μl Fc Block (BD Pharmingen, 553141) per sample followed by 10 min incubation on ice ...
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bioRxiv - Microbiology 2023Quote: ... 25 μL of control serum and 25 µL of serum pooled from all mice within a treatment group were pipetted onto a 2-mm diameter plain paper disc (BD) and allowed to dry for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... For microscopy experiments SDC-lysine media was used (2% glucose, 6.75 g/l yeast nitrogen base without amino acids (291929, BD Difco), 1.92 g/l yeast synthetic drop-out media supplements without lysine (Y1896 ...
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bioRxiv - Immunology 2023Quote: Bone marrow was isolated from dissected femurs that were gently crushed in PBS with 2% (v/v) FBS and subsequently filtered over a 70μm cell strainer (BD Falcon). Red cell lysis was done by incubating samples with ACK lysis buffer (Quality Biological ...
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bioRxiv - Immunology 2023Quote: ... The protein binding capacity of the well surface was saturated with 300 μL blocking solution (PBS supplemented with 2% skim milk powder (BD) and 0.1% Tween 20 ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) and analyzed on a BD FACS Aria III (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... A total of 2 × 106 vector control or shACSL4 cells were mixed 1:1 by volume with Matrigel (BD Biosciences). Each mouse was injected orthotopically into both the flanks ...
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bioRxiv - Immunology 2023Quote: ... stained cells were resuspended in PBS 2% FBS and DC1 were purified in a FACS Aria II Cell Sorter (BD).
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bioRxiv - Cell Biology 2023Quote: Cytokine released by the SARS-CoV-2-infected or ApoBD-treated MDMs were measured using human cytokine bead array as per manufacturer’s instruction (BD Biosciences). Briefly ...
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bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with pre-optimized concentrations of the following antibodies: PE anti-human IgD (clone IA6-2, BD Biosciences), PE-TXRD anti-human CD10 (clone HI10A ...
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bioRxiv - Cancer Biology 2023Quote: ... Leukemic cell infiltration was evaluated using immuno-labeling with anti-huCD45 and anti-huCD7 antibodies (see Supplementary Table 2) and a FACS-Canto II flow cytometer (Becton Dickinson, BD). Cells from Femur ...
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bioRxiv - Microbiology 2023Quote: ... yeast isolates were grown overnight for 16 h from a single colony in 2 mL PD broth (BD, Sparks MA) with shaking at 225 rpm ...
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bioRxiv - Immunology 2024Quote: ... Cytokine staining was performed with 2 x 106 cells after 3.5h of in vitro stimulation in Opti-MEM media supplemented with Golgiplug (BD Biosciences), 10 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: The titles and abstracts of 2 341 articles retrieved from the preliminary search were screened and cross-checked by two independent reviewers (ZL and BD) and discrepancies were resolved through discussion with a third researcher (DM) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 × 106 cells were harvested and resuspended in 100 μl of ice-cold 20% matrigel (BD Bioscience, Franklin Lakes, NJ) in PBS (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Immune cells were resuspended in surface staining mix containing FACS buffer with 1/2 of final volume of Brilliant Stain Buffer (BD), 50 μg/ml anti-mouse CD16/CD32 (2.4G2 ...
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bioRxiv - Pathology 2024Quote: ... Lentivirally transduced LX-2 cells were sub-cultured until confluent before being flow-sorted on the BD FACSAriaTM (BD Biosciences) to obtain cells with top 10 % highest eGFP expression ...
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bioRxiv - Cell Biology 2024Quote: Twenty µl of platelet post activation supernatants were incubated with 2.5 µl of phycoerythrin (PE)-conjugated mouse anti-human CD62P (clone AK-2, BD Biosciences), 2.5 µl of PE-conjugated CD61 (clone VI-PL2 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were blocked for 30 minutes in FACS buffer (2% FBS 1X PBS) containing Fc Block anti-CD16/32 (2.4G2, BD Biosciences) (1:100 dilution) ...
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bioRxiv - Immunology 2024Quote: ... and then stained for 30 min at 4°C in PBA buffer (PBS, 0.5% BSA, 2 mM EDTA) in the presence of Fc Block (1:50 dilution, BD-Biosciences), using the following antibody combination ...
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bioRxiv - Cancer Biology 2024Quote: ... with 4′,6-diamidino-2-phenylindole (DAPI) as a viability dye and the following antibodies: anti-CD31 PE (BD Biosciences), anti-CD45 APC (Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... then were seeded into a 96-well plate at a density of 5×103cells/well coated with 10mg/ml Matrigel matrix without growth factor (Cat No: 3432-010-01, BD Biosciences, CA, USA) for 6 hour incubation at 37□ ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Cancer Biology 2020Quote: ... 2000 cells were plated in 8-well chamber slides onto a base layer of growth factor-reduced Matrigel (5 mg/ml, BD Biosciences, Lot A6532) and collagen type I (1.5-2.1 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The seeded cells (5 x 104 cells/mL) were pre-stained with 1 μL Orange NO probe (BD Biosciences, San Jose, CA, USA) per 500 μL cell suspension for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Assay plates were incubated overnight at 37ºC in a 5% CO2 incubator and developed using BD ELISPOT AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISPOT reader and Immunospot software (Cellular Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked in PBS + 5% BSA for 1 hour before addition of primary antibodies (anti-LC3A/B, CST #4108, 1:100; anti-LAMP1, BD #555798, 1:100) diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, New Jersey, USA). Unless otherwise stated ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Plant Biology 2019Quote: Surface-sterilized seeds were germinated and grown for 5 days containing 1% w/v sucrose and 1% w/v agar (Difco Bacto agar, BD laboratories; http://www.bd.com) in a growth chamber (NK system ...
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bioRxiv - Microbiology 2019Quote: Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, New Jersey, USA). Colonies were then picked and inoculated in fresh Columbia broth (Remel Microbiology Products ...
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bioRxiv - Microbiology 2021Quote: ... Off-line samples were taken from the reactors each 5-10 h and the analysis of the GFP expression levels was performed with a BD Accuri C6 (BD Biosciences, CA, USA) based on FL1-A channel (excitation 488 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... the BECs were seeded in 96-well plates coated with collagen type I (5 µg/mL, #354231, BD Bioscience, San Jose, CA, USA) at a density of 104 cells per well ...
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bioRxiv - Immunology 2021Quote: ... were resuspended in defRPMI-1640 with 3% newborn calf serum and Benzonase™ (FACS buffer) in 5 mL FACS tubes and pre-incubated with GolgiStop™ (BD Biosciences) for ∼60 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... both ends of femurs and tibiae were cut with surgical scissors and bone marrow was extracted by flushing 3-5 ml sterile and ice-cold buffer through the bones using a 26G needle (BD Microlance 3 300300). The isolated bone marrow was then filtered through a 40 µM nylon cell strainer (FALCON 352340 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...
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bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88; and IL-5, BD Biosciences, #51-1806KZ) was added at a 1:250 dilution in ELISpot diluent (eBioscience ...
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bioRxiv - Microbiology 2022Quote: ... 98 mL of fresh MRS medium is then inoculated with 2 mL of the overnight culture and incubated at 37°C without shaking to an OD of 0.5-0.6 (ca. 4-5 hours) in a GasPak anaerobic jar (BD GasPak™ EZ container systems). Cells are harvested a first time by centrifugation at 4,000 g for 5 min ...