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Citations for Becton, Dickinson and Company :
1701 - 1750 of 1962 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... hPSCs were subject to routine pluripotency using BD Stemflow Human and Mouse Pluripotent Stem Cell Analysis Kit (BD Biosciences, 560477) as recommended by the manufacturers ...
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bioRxiv - Biochemistry 2021Quote: ... Apoptotic cells were detected by Accuri C6 fluorescence-activated cell sorting (FACS) using Annexin V-APC Apoptosis Detection Kit (BD #), according to the manufacturers protocol.
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bioRxiv - Cancer Biology 2020Quote: ... to analyze cell cycle distribution and we quantified apoptotic cells with PE-AnnexinV Apoptosis Detection Kit I (559763, BD Pharmingen).
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bioRxiv - Developmental Biology 2021Quote: ... Single cell whole transcriptome analysis libraries were prepared according to the manufacturer’s instructions using BD Rhapsody WTA Reagent kit (BD, 633802) and sequenced on the Illumina NextSeq 500 using High Output Kit v2.5 (150 cycles ...
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bioRxiv - Cancer Biology 2021Quote: Viability of AML cells was measured using FITC Annexin V Apoptosis Detection Kit I (BD Biosciences, San Jose, CA, USA). Briefly ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for viability and surface markers followed by intracellular cytokine staining using the Cytofix/Cytoperm kit from BD Biosciences (Franklin Lakes ...
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bioRxiv - Immunology 2021Quote: In vivo cytokines from the serum/plasma of mice were measured using the Cytometric Bead Array Human Th1/Th2 Cytokine Kit (BD), following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: Murine IFNγ and IL-4 ELISPOT assays were carried out according to the manufacture’s protocols for mouse IFNγ and IL-4 ELISPOT kit (BD Bioscience). Immunized mice splenocytes were seeded in the plates with a density of 2 × 105 cells per well and incubated with the peptide pool of 15 mer peptides with 11 overlapping amino acids for SARS-CoV-2 RBD protein (5 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: Cell apoptosis assay was performed by flow cytometry using an Annexin V-FITC /propidium iodide(PI) apoptosis detection kit(BD). Cells were seeded in triplicates in 6-well plate at the density of 2×105 cell per well ...
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bioRxiv - Cancer Biology 2022Quote: ... and MDAMB231-NS cells separately with the Sample-Tags from the BD Human Immune Single-Cell Multiplexing Kit (BD Biosciences) as described in the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... with LCMV Cl13 and BrdU detection in splenic P14 cells was performed one day later (d8p.i.) using a BrdU detection Kit (BD Bioscience) according to manufacturer’s protocol.
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bioRxiv - Genomics 2022Quote: ... Single cell capture and cDNA synthesis using the Single Cell Capture and cDNA Synthesis kit (#633731, #633733, #633773) were done following the manufacturer’s protocol (BD Biosciences). Libraries were then prepared using the BD Rhapsody WTA Amplification Kit (#633801 ...
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bioRxiv - Immunology 2022Quote: ... Cells were next washed twice with cold 1x Perm/WashTM buffer (Cytofix/Cytoperm™ fixation and permeabilization kit, BD Biosciences) and incubated with a master mix containing 95μL of 1x Perm/WashTM buffer and 5μL of the intracellular marker Ki-67 (Table 2 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was collected to determine the levels of MCP-1/CCL2 using commercial kit (BD Biosciences, CA, USA, Cat# 555179) as per manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... Mice sera were collected retro-orbitally at 4 h and 7 days post administration and subjected to cytokine analysis (Cytometric Bead Array Mouse Inflammation Kit, BD) using flow cytometry following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cell cycle and cell apoptosis assays were performed according to the manufacturer’s instruction (FITC Annexin V Apoptosis Detection Kit I, BD, 556547; PI/RNase staining buffer, BD, 550825).
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bioRxiv - Immunology 2024Quote: Plasma samples isolated from blood collected at day 7 and previously frozen at -20°C were thawed and pooled to reach the minimum sample volume required for cytokine analysis (20μL) using the Th1/Th2/Th17 cytokine kit (BD Biosciences). Data were analyzed using the FCAP array software ...
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bioRxiv - Microbiology 2024Quote: ... Secreted cytokines were measured using a BD Th1/Th2/ Th17 cytometric bead array (CBA) kit (BD Biosciences cat no. 560485) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... BrdU incorporation was assessed in splenic T cells 24 hours after injection using a BrdU flow cytometry kit (BD PharmingenTM).
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bioRxiv - Immunology 2024Quote: Levels of inflammatory cytokines in sera were measured using a cytometric bead array (CBA) mouse inflammation kit (552364, BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... the supernatants were collected for cytokine determination using ELISA kits according to the manufacturer’s recommendations (R&D and BD Bioscience).
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bioRxiv - Immunology 2023Quote: ... Intracellular staining for cytoplasmic and nuclear proteins were performed with Transcription Factor Staining Buffer kit according to manufacturer’s instructions (BD Pharmingen). Dead cells were excluded by DAPI (BioLegend ...
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bioRxiv - Systems Biology 2023Quote: Brdu staining was used to analyze the cancer cell proliferation according to manufacturer the protocol (Brdu APC flow Kit, 552598, BD). Briefly ...
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bioRxiv - Immunology 2022Quote: ... the cells were analyzed by bromodeoxyuridine (BrdU) incorporation and DNA content staining with APC BrdU Flow Kit (BD Biosciences, #552598) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was performed after 15 minutes fixation using BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (BD Biosciences, #554714). Flow cytometry data were recorded on BD LSRII or FortessaX20 using DIVA software and analyzed using FlowJo™ software (Tree Star).
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bioRxiv - Microbiology 2023Quote: The release of IL-10 and IL-6 were quantified in cell supernatants using specific immunoassay kits purchased from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cytokine production (IFN, IL-4, IL-17A, IL-10) was also assessed by Cytometric Bead Array (CBA) Kit (BD Biosciences) on supernatants collected at day 3 of polarization ...
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bioRxiv - Neuroscience 2023Quote: ... supernatant was incubated with cytokine beads (CBA; Multiplex magnetic bead-based antibody detection kits, BD Biosciences, CA, USA, Cat# 551811) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization kit according to the manufacturer’s instructions (BD Biosciences). The presence of intracellular capsid protein VP1 was determined by staining with polyclonal rabbit anti-EV-D68 VP1 (10 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... permeabilised with Foxp3 transcriptional factor staining buffer kit (eBioscience) before intracellular staining with appropriate cytokine antibodies and acquisition through LSR Fortessa machine (BD Immunocytometry system ...
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bioRxiv - Cell Biology 2023Quote: ... The BrdU pulse was followed by a 3-weeks chase period to detect by flow cytometry label-retaining cells by combining surface staining to define MPPs with intracellular staining using the BrdU-FITC labeling kit following the manufacturer’s instructions (BD Biosciences). Apoptosis was measured using the Annexin V detection kit (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Intracellular expression of cytokines and transcription factors was analyzed using the respective fluorochrome-coupled mAbs in conjunction with either the BD Cytofix/Cytoperm kit (BD) or the Foxp3 staining buffer set (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with 500μL BrdU (2mg/ml) intraperitoneally 12h prior to analysis using a BrdU flow kit (BD Biosciences) as previously 52 ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... the cells were subjecte to intracellular staining with BD Cytofix/Cytoperm buffer kit according to the manufactures’s instruction (BD BioScience). Cells were analyzed with the MACSQuant cell analyzer (3 lasers ...
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bioRxiv - Bioengineering 2022Quote: ... and MHCII-NYESO conjugated on the SCMs are quantified by the PE Phycoerythrin Fluorescence Quantitation Kit (BD Biosciences, Cat # 340495). Each test in the kit comes with 4 different intensities of PE-labeled beads ...
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bioRxiv - Immunology 2023Quote: ... 1 x 105 HSC-F cells were infected with SIVmac239 at MOI 0.05 in U-bottomed plates and permeated with Cytofix/Perm kit (BD Biosciences). After two washes ...
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bioRxiv - Neuroscience 2024Quote: ... Intracellular markers were stained after fixation and permeabilization of cells using an Intracellular or Phospho-Flow Staining kit (BD Biosciences). FACS data were acquired on a Northern Lights spectral flow cytometer (Cytek ...
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bioRxiv - Immunology 2023Quote: Phagocytic potential of blood-derived monocytes and granulocytes was examined immediately after necropsy by Phagotest and Phagoburst kits (BD Biosciences). Phagocytosis was examined by stimulating blood samples with opsonized E ...
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bioRxiv - Microbiology 2023Quote: ... The cytokine level from the plasma was quantitated using cytometric bead array kit (P110100403, JiangXi Cellgene) on Flow Cytometry (FASCantoII, BD). All measurements were handled according to the manufacturer instruction ...
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bioRxiv - Cell Biology 2023Quote: Cells were analyzed for cell cycle phase using a ClickIT EdU Alexa Fluor 647 kit (Fisher) and FxCycle PI/RNase Staining Solution (Fisher) followed by flow cytometry on an LSR-II (BD) and analysis using FlowJo.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice with ice-cold PBS and FITC-annexin-PI was done as per manufacturer’s instruction using FITC Annexin V Apoptosis Detection Kit I (Cat. 556547, BD Biosciences). Flow cytometry was performed using a BD LSR II flow cytometer of the Flow Cytometry Core Facility at The Bloomberg School of Public Health ...
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bioRxiv - Bioengineering 2024Quote: ... in vitro transcription and translation was completed using the Proteoliposome BD (Bilayer and Dialysis) kit from CellFree Sciences (Product# CFS-CPLE-BD). The transcription reaction was completed at 37°C for 6-hours using a thermocycler ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... Anti-TCR Vα2-PE (# 553289), anti-CD8α-BUV395 (# 563786), Stain Buffer (FBS, # 554656) and BD Cytofix/Cytoperm kit (# 554714) were purchased from BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Naïve and TdLN single-cell suspensions were labeled sequentially using the mouse Single Cell Sample Multiplexing Kit (BD Biosciences, #633793) and BD AbSeq Ab-Oligos targeting αCD11b (#940008 M1/70) ...
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bioRxiv - Developmental Biology 2024Quote: ... Single cell whole transcriptome analysis libraries were prepared according to the manufacturer’s instructions using BD Rhapsody WTA Reagent kit (BD, 633802) and sequenced on the Illumina NextSeq 500 using High Output Kit v2.5 (150 cycles ...
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bioRxiv - Genomics 2024Quote: ... RoCKseq beads were resuspended in 680 µl Sample Buffer (Cat. No. 650000062, BD RhapsodyTM Enhanced Cartridge Reagent Kit, BD 664887) instead of 750 µl.
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bioRxiv - Cancer Biology 2024Quote: ... and whole-transcriptome and Sample-tag libraries were obtained using the BD WTA amplification kit and protocol (BD Biosciences, #633801). The quality of the final libraries was assessed by using an Agilent 2200 TapeStation with HS D5000 ScreenTape (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Rictor or mTOR silenced were collected for apoptosis assay using PE Annexin V Apoptosis Detection Kit I (BD Biosciences Cat#559763). Cells were analyzed using CytoFLEX Flow Cytometer.
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bioRxiv - Cell Biology 2020Quote: ... and macrophage chemoattractant protein-1 (MCP-1) were quantified by bead-based flow cytometry assay (CBA Kit; BD Biosciences, Heidelberg, Germany) in accordance with the instructions of the manufacturer.
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bioRxiv - Immunology 2021Quote: ... Cells for intracellular staining of HLA-DR were first fixed and permeabilised using BD Cytofix/Cytoperm™ Plus Fixation/Permeabilization Kit (BD Biosciences ...