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Citations for Becton, Dickinson and Company :
1701 - 1750 of 3459 citations for DHEA S ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The concentration of antibody was quantified in plates coated in triplicate with two-fold dilutions of an appropriate purified mouse Ig isotype standard (IgG and IgA; BD Biosciences), starting at 0.5 μg/μl ...
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bioRxiv - Immunology 2020Quote: ... fibroblast-collagen cultures were prepared in Falcon cell culture inserts and placed into Falcon 6 Well Deep Well TC-Treated Polystyrene Plates (BD Biosciences) containing 2×105 fibroblasts in FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... in 96-well round bottom tissue culture plates in R10 media with the presence of Golgi inhibitors monensin and brefeldin A (BD Bioscience) to reach 200μL per well ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μL/well were plated in triplicate into a 96-well plate pre-coated with 50 μL of bacto-agar (BD Biosciences) in PBS1x (0,66% w/v) ...
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bioRxiv - Immunology 2022Quote: ... 0.75 × 105 naïve T cells were cultured for one or two days with plate-bound anti-CD3ε (2 µg/ml, clone 145-2C11; BD Biosciences) in differentiation medium consisting of RPMI1640 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... and then tetramer+CD8+7-AAD- cells were sorted into 96-well plates (NIPPON Genetics, Cat# 4ti-0770/C) by using an FACS Aria II (BD Biosciences). TCRα and TCRβ cDNA pairs were amplified from single T cells by a one-step multiplex RT-PCR method described in our previous study19 ...
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bioRxiv - Immunology 2022Quote: Igλ-enriched B1-8 cells were cultured for 2-3 days at 4 *105 cells/well in a 12-well tissue culture plate (BD Falcon) and then washed ...
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bioRxiv - Immunology 2022Quote: ... Eventually 200 000 CD8+ T cells from vaccinated animals were seeded with 50 000 ovalbumin pulsed splenocytes on IFNγ ELISpot assay plates (BD Biosciences). The cells were incubated for 48 hours and the plates were developed as described by the manufacturer.
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bioRxiv - Immunology 2022Quote: ... Tracheas were stretched out by pinning to SylGard-coated well of 6 well plate and fixed for 1 hr on ice in Cytofix/Cytoperm buffer (BD Biosciences). Immunofluorescent staining was performed in PBS with 0.25% Triton X-100 at 4°C as follows ...
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bioRxiv - Cancer Biology 2019Quote: 24-well plate inserts were coated with Matrigel and cells were plated on the surface of the Matrigel (BD Sciences 356231) in serum free media ...
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bioRxiv - Genetics 2020Quote: Human embryonic stem (hES) H1 cells (WA01, WiCell) were maintained under feeder free conditions on Matrigel-coated plates (#354277, BD Biosiences) in mTeSR™1 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were seeded into the center 60 wells of a 96 well plate (Trueline) at precise initial cell numbers using Fluorescence Activated Cell Sorting (BD Fusion) plate sorting at single cell precision ...
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bioRxiv - Microbiology 2020Quote: ... Parasites expressing the transfected Cas9-GFP were sorted by flow cytometry into 96 well plates containing HFF monolayers using a BD Influx cell sorter (BD Biosciences). This line was used for complementing pSFP1myc constructs ...
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bioRxiv - Molecular Biology 2020Quote: ... the average of the fluorescent intensity was calculated as the proteasome activity as follows: 30 µl of each fraction sample was transferred to a 96-well BD Falcon microtiter plate (BD Biosciences), and mixed with 2 mM ATP and 0.1 mM fluorogenic peptide substrate Suc-Leu-Leu-Val-Tyr-AMC (Peptide Institute) ...
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bioRxiv - Microbiology 2020Quote: ... The bacterial strains were stored in 10 % (V/V) glycerol stocks at −80 °C and freshly streaked on blood agar plates (BD Diagnostics) followed by their overnight growth at 35 °C before use ...
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bioRxiv - Molecular Biology 2020Quote: All mycobacterial strains were grown on Middlebrook 7H10 plates (Difco) containing OADC supplement (oleic acid, albumin, dextrose and catalase; BD Biosciences) or liquid 7H9 medium containing ADC supplement (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... were resuspended in D-PBS supplemented with 2% (v/v) FBS containing the antibody cocktail in 96-well microtiter plates (BD Biosciences) for 20 min on ice in the dark ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293 cells transfected with ABE plasmids were collected from 6-well plate and analyzed on Becton Dickinson LSR II (BD Biosciences) to determine GFP-positive cells ...
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bioRxiv - Cell Biology 2019Quote: ... GFP+ cells were sorted in 96-well plates 48 h post transfection using a FACS Aria III cell sorter (BD BioSciences). Single cells were grown until formation of viable individual clone in DMEM medium supplemented with 50% fetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Physiology 2019Quote: ... a vial of frozen cells was revived and cells sorted as singlets into 384-well plates using a BSL3 BD FACSAria Cell Sorter (BD Biosciences). Clones that grew to confluency were further split 1:3 ...
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bioRxiv - Molecular Biology 2020Quote: ... Single nuclei with low Hoechst/PI fluorescence (G1 population) were sorted into 96-well plates containing freezing buffer using a FACSJazz (BD Biosciences). Pre-amplification-free single-cell whole genome sequencing libraries were prepared using a Bravo Automated Liquid Handling Platform (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and monoclonal cell lines were generated by isolating cells expressing both TauRD fusion proteins in a 96 well-plate by cell sorting with a BD FACS Aria III (BD Biosciences) following amplification ...
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bioRxiv - Immunology 2021Quote: ... patient lymph nodes cell suspension (around 30×106 cells/well in a U-bottom 96 well plate; BD Falcon, Cat# 3077) were washed once with 200 mL FACS buffer (4% FBS ...
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bioRxiv - Plant Biology 2020Quote: In vitro mycelium diameter measurements were performed by depositing a plug of mycelium (ø 0.6 cm) in the center of ½ strength V8 agar plates (1.5 % granulated agar, BD Difco™) supplemented with increasing concentrations of D-glucose (Duchefa Biochemie ...
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bioRxiv - Cancer Biology 2021Quote: ... 15000 HUVEC cells were seeded per well of a 96 well plate on a layer of matrigel (BD Biosciences, Bedford, MA). Cells were treated with condition media from HCT116 or DLD1 ...
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bioRxiv - Neuroscience 2021Quote: ... The dissected tissue was mechanically triturated by a fire-polished glass pipette and plated onto 24-well plates coated with 2% laminin (BD Biosciences) and 1% poly-D-lysine (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected with 5μg/ml blasticidin and bulk cells expressing the TetR protein were clonally isolated in 96-well plates using FACS (BD-Aria). BRAFV600E and BRAFWT were expressed in cells using Gateway-compatible lentiviral constructs (pLenti CMV/TO puro DEST ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots of 100μl of serial 10-fold dilution of the oral and nasal wash were inoculated into brain heart infusion agar plates (BD 252109). After incubation at 37°C overnight under the aerobic conditions ...
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bioRxiv - Microbiology 2021Quote: ... Leaf press cultures were done by placing birch leaves on the surface of a plate containing 0.2x potato dextrose broth (PDB; BD Difco; www.fishersci.com) with 15g/l agar (PDA) ...
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bioRxiv - Immunology 2021Quote: ... Resting- or in vitro-generated effector CD4+ or CD8+ T cells (105 cells/well) were stimulated with 1 μg/ml plate-bound anti-mouse CD3 mAb (BD Bioscience) in combination with different concentrations of P3CSK4 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell suspensions were filtered using a 100 μm plate filter immediately prior to analysis on an LSRII flow cytometer (BD Biosciences) or an EC800 flow cytometer (Sony) ...
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bioRxiv - Genomics 2021Quote: Healthy human hiPSC cell lines for Figure 4 were cultured under feeder-free conditions on plates coated with human-qualified Matrigel (BD Biosciences) diluted 1:40 in mTeSR-1 medium (StemCell Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Single cells were index sorted into 384 well plates containing 1uL of reverse transcription (RT) master mix using a FACS Aria III with FACS Diva software (BD Biosciences) to maintain protein expression with plate well location ...
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bioRxiv - Microbiology 2021Quote: ... Second-passage cells were grown to confluency in 24- or 96-well tissue culture plates (Costar, Van Nuys, CA) on fibronectin (BD Biosciences). All incubations were in 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... the cell suspension was then incubated on two PDGFRα plates coated with rat anti-PDGFRα IgG (BD Biosciences, 558774; 1:500). The adherent cells were washed with PBS to remove all antigen-negative nonadherent cells ...
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bioRxiv - Cell Biology 2022Quote: ... the endothelial cell monolayers in 6-well plates were lysed after removing center region using cell scraper (BD Falcon #35-3085) and washing with 1X HBSS (Corning #21-022-CV).
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bioRxiv - Neuroscience 2022Quote: SH-SY5Y cells were seeded at a density of 4×103 cells/well in flat bottom 96-well plates previously coated with Matrigel (BD, Biosciences), and 10 μM all-trans-retinoic acid (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and GFP-positive single cells were sorted by FACS into 96-well plates using a FACSAria III cell sorter (BD Biosciences) to obtain single cell-derived clones ...
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bioRxiv - Developmental Biology 2022Quote: iPSCs (WTC with dCas9-KRAB, AICS90) and hESCs (H1 line, WAe001-A) were maintained under feeder free conditions on Matrigel-coated plates (#354277, BD Biosciences) in mTeSR™1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary AML cells were harvested and stained with human CD45 and cells were analyzed by a high-throughput automated plate reader (BD LSRFortessa). Dead cells were excluded using the Near-IR Live/Dead marker (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Unfractionated BM was diluted 1:1 in DMEM with 10% FBS and added to vacuum gas plasma treated plates (BD Falcon). After three days ...
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bioRxiv - Genomics 2022Quote: ... Forty-eight hours after transfection single GFP+ cells were sorted into 96-wells plates on FACS ARIA II/III Flow Cytometer (BD Biosciences) and expanded ...
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bioRxiv - Synthetic Biology 2022Quote: ... thermoglucosidasius DSM2542T was routinely grown on 2-SPY medium106 at 60 °C with 150 RPM shaking or on Tryptic Soy Agar plates (BD Biosciences) at 60 °C ...
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bioRxiv - Bioengineering 2024Quote: ... cells were analyzed and mCherry positive clones were FACS sorted as single cells in 96-well plates using ARIAIII (BD Biosciences). Successfully expanded clones were then further characterized.
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bioRxiv - Genomics 2024Quote: ... Three to five 96-well plates were seeded with one fluorescent cell per well using the BD FACSAriaTM II (BD Biosciences), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and NR021 were cultured on Columbia sheep blood agar plates for 48 hours at 37°C under anaerobic conditions (GasPak™ EZ Anaerobe Gas Generating Pouch System with Indicator, BD) from isolates stored at -80°C ...
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bioRxiv - Cell Biology 2024Quote: ... Suspensions were kept at 4°C in darkness until required and were filtered using a 100 μm plate filter prior to loading on an LSRII flow cytometer (BD Biosciences). Gates were applied and density curves generated using FlowJo V10 ...
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bioRxiv - Cell Biology 2024Quote: ... Electroporated cells were cultured in 24-well plates for 5-7 days and transferred to 6-well plates prior to selection by fluorescence-activated single cell sorting using FACS Melody (BD Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... Cleaned coverslips were removed from 70% ethanol and allowed to dry in a biosafety cabinet then were placed into the wells of a 6 well tissue culture plate and coated with 10 µg/ml fibronectin (BD Biosciences) in PBS (10.1 mM Sodium phosphate dibasic ...
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bioRxiv - Immunology 2024Quote: ... and 5×104 of each were combined in 96-well U-bottom plates with anti-CD40 (100 ng/mL, clone HM40-3, BD Biosciences) in the presence or absence of OVA (10 μg/mL ...