Labshake search
Citations for Becton, Dickinson and Company :
1701 - 1750 of 7027 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: ... cells were collected and fixed in 4% paraformaldehyde prior to acquisition on a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA). CFSE signal intensity of 1 × 104 cells was recorded and analyzed to measure cell divisions ...
-
bioRxiv - Bioengineering 2020Quote: ... was applied for 30 minutes at 4°C and stained cells were resuspended in FACS buffer before flow analysis on a BD FACSCanto™ II (BD Biosciences). Gating and flow data were processed on FlowJo (FlowJo LLC).
-
bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII flow cytometer (BD Bioscience, San Jose, CA).
-
bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA).
-
bioRxiv - Molecular Biology 2020Quote: ... Then the syndecan-4 expression on HUVECs was measured by flow cytometry with a BD Biosciences LSR II (BD Biosciences, CA, USA).
-
bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
-
bioRxiv - Immunology 2019Quote: Cells were harvested by centrifugation at 400 g for 5 minutes at 4°C followed by staining with fixable viability stain (BD Horizon FVS700) for 10 minutes on ice in PBS ...
-
bioRxiv - Cancer Biology 2020Quote: Plasma serum was collected from 4-6 drops of peripheral blood obtained from NSGS mice via submandibular bleeding using Microtainer Collection Tubes (BD Microtainer, # 365967). Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29 ...
-
bioRxiv - Neuroscience 2021Quote: ... A 1ml astrocyte cell suspension of 4×104 cells/ml was plated in three chambers of four-chamber glass slides (BD Falcon, USA) coated with poly-D-lysine ...
-
bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
-
bioRxiv - Immunology 2020Quote: ... in house production), 10 μg/mL α-IL-4 (cl. 11B11, in house production) and 10 ng/mL IL-12 (BD Pharmingen) and cultured on goat α-hamster IgG (MP Biochemicals ...
-
bioRxiv - Immunology 2019Quote: ... Cells were resuspended in PBS/5%FBS then fixed in an equal volume of 1% paraformaldehyde and stored in the dark at 4°C prior to analysis on a BD LSR FortessaTM or LSR2 flow cytometer (BD Biosciences, UK). Data were analysed using FlowJo software (FlowJo LLC ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were fixed at 4 °C for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 °C for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer ...
-
bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...
-
bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
-
bioRxiv - Immunology 2023Quote: ... Stimulated cells were washed once in FACS buffer before fixation for 20 min at 4°C using the BD Cytofix/Cytoperm solution and permeabilization using BD Perm/Wash™ buffer (BD Biosciences). Permeabilized cells were incubated for 30 min with antibodies (diluted 1:200 in BD Perm/Wash ...
-
bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... treatment was performed at 100μg/ml concentration in dark at 4°C for an hour and analyzed by FACS Calibur flow cytometer and processed using Cell Quest software (BD Biosciences, USA).
-
bioRxiv - Cancer Biology 2022Quote: ... 25 μL medium containing 1 × 106 luciferase-expressing MCF10AER/vSrc cells (control or 4-OHT transformed, with or without NLS-catalase expression) were mixed with 75 μL Matrigel matrix (BD Bioscience, #356237) containing 10 μM 4-OHT and injected into the inguinal mammary fat pad of the female mice at 6 to 8 weeks of age ...
-
bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
-
bioRxiv - Genomics 2023Quote: Whole blood aliquots at a volume of four (4) milliliters were collected from participants using sterile Vacutainer blood collection kits (BD Vacutainer®) and subsequently biobanked at −80°C for DNA extraction ...
-
bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... Blood was collected via submandibular bleeds with goldenrod 4 mm lancets collected into sample tubes coated with K2 EDTA (BD Ref# 365974) and 60 μL of blood was lysed with 500 uL of ACK lysis buffer ...
-
bioRxiv - Genomics 2020Quote: ... each anaesthetised snail was injected with 170 μL of snail saline (see Standen 1975) in the head-foot using a syringe and needle (0.45 mm × 16 mm BD Microlane™ 3, New Jersey, USA). This treatment examined the effect of wounding in activating immune defence when compared to anaesthesia without exposure to immune elicitors (treatment 2 above) ...
-
Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... NP-FB23-2 (3 mg/kg) was delivered through the retro-orbital sinus into neonatal mice with a 31-gauge insulin syringe (BD Biosciences, San Jose, CA, USA).
-
bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 1 h with primary antibodies to RAD51 (B-bridge International, #70-001) and to cyclin A (BD Biosciences, # 611268) diluted in 1% BSA ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissues were blocked with 5% donkey serum and 0.1% Triton X-100 in PBS for one hour at room temperature and incubated with 1:1000 of anti-CD47 (BD Biosciences), 1:500 of anti-TSP1 (Abcam) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were then spun down and resuspended in 100LμL of buffer containing fluorescently conjugated antibodies for one hour (5LμL of each antibody per sample): APC-Cy7 Mouse Anti- Human CD4 (1:20 dilution; BD Biosciences, #557871), APC-Cy7 Mouse IgG1 ...
-
bioRxiv - Immunology 2021Quote: ... Anti-Granzyme B (GB11) and anti-Ki67 (B56) were from BD Pharmingen ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were incubated with 100 ul Permeabilization buffer B (BD Biosciences) prior to staining with the anti-FLAG and Donkey/Mouse Alexa488 antibodies as described above ...
-
bioRxiv - Immunology 2021Quote: ... S+ memory B cells were single sorted (BD FACSAria™ Fusion) into 96 well PCR plates as described before (Chen et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... Acid citrate dextrose (ACD) tubes (Sol. B, Vacutainer®, BD, Switzerland); coverslips (18 mm diameter ...
-
bioRxiv - Neuroscience 2021Quote: ... Black lines represent the rat’s body direction (BD as in B), and green and blue dots represent head-mounted LEDs ...
-
bioRxiv - Immunology 2022Quote: ... IgG1 or IgG2a/b antibodies (BD Biosciences San Jose, CA, USA) at a dilution of 1:400 in 1% BSA/PBS were added and incubated for 2 h at RT on a shaker ...
-
bioRxiv - Immunology 2020Quote: ... B-cell proliferation was monitored by intracellular Ki67 AF488 (BD Biosciences) using buffers in the Fix & Perm© Cell Permeabilization Kit (BD Biosciences) ...
-
bioRxiv - Immunology 2020Quote: ... Naïve B cells were sorted on a FACSAria flow cytometer (BD) by including cells with CD19+ ...
-
bioRxiv - Immunology 2020Quote: ... B cell subtypes were bulk sorted on a FACSAriaIII (BD bioscience) and collected in PBS directly followed by a RNA extraction step (Qiagen RNeasy Plus Micro Kit ...
-
bioRxiv - Immunology 2023Quote: ... GC B cells were enriched by CD43 (clone S7, BD Biosciences), IgD (clone 11-26c ...
-
bioRxiv - Immunology 2023Quote: ... B cells were stained with VPD450 at 3.5uM (BD Biosciences 562158). Cells were stimulated with BLYS at 50ng/ml and with an anti-IgM F(ab’)2 at 15ug/ml ...
-
bioRxiv - Immunology 2023Quote: ... Anti-Granzyme B (GB11) and anti-Ki67 (B56) were from BD Pharmingen ...
-
bioRxiv - Bioengineering 2023Quote: ... IFN-γ and granzyme B and read by flow cytometry (BD LSR II or BD LSRFortessa™ ...
-
bioRxiv - Cell Biology 2023Quote: Mouse anti-human CD11c-BV650 (Clone: B-ly6; BD Biosciences, 563404), Verified Reactivity ...
-
bioRxiv - Cell Biology 2023Quote: Mouse anti-human CD11c-BV650 (Clone: B-ly6; BD Biosciences, 563404), Verified Reactivity ...
-
bioRxiv - Immunology 2024Quote: ... B cells were sorted on a FACS Aria II (BD Biosciences) cell sorter and collected in RMPI 1640 media supplemented with 20% FBS ...
-
bioRxiv - Immunology 2024Quote: ... T and B cell Activation Antigen (GL7) PE (561530, BD Pharmingen), CD95 PE-Cy7 (557653 ...
-
bioRxiv - Immunology 2019Quote: ... One hundred μL of EasySep Mouse Streptavidin Rapidspheres (BD Biosciences) were added per 1×108 splenocytes ...
-
bioRxiv - Immunology 2022Quote: ... followed by one wash with perm wash (BD #51-2091KZ) and one wash with PBS ...
-
bioRxiv - Biophysics 2022Quote: ... One hole should snugly fit PE20 tubing (BD Intramedic #427406), and the second hold should fit PE60 tubing (BD Intramedic #427416 ...
-
bioRxiv - Neuroscience 2019Quote: ... injection with α-Ly6G or isotype control antibodies (IP 4 mg/kg; α-Ly6G, clone 1A8, No. 561005; Iso-Ctr, Rat IgG2a, κ, No. 553929; BD Biosciences). Animals were 9-10 months of age at the start of the experiment.