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Citations for Becton, Dickinson and Company :
1651 - 1700 of 6650 citations for 6 Chloro 4 methyl 1 benzothiophen 3 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Cell suspensions were washed and re-suspended in PBS at 4°C and analysed using a BD 5L LSR Fortessa and BD FACSDiva software (BD Biosciences). To exclude dead cells ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... for 20 minutes and then with a blood antibody cocktail for 30 minutes in the dark at 4 degrees: anti-CD45 BB515 (BD Horizon), anti-CD14 PerCP-Cyanine5.5 (eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... 1 x 106 popliteal LN cells/ml were stimulated with 10 μg/ml of PHA for 4 days and cytokines levels were measured by FACS using Cytometric Bead Assay (CBA; BD bioscience).
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bioRxiv - Neuroscience 2021Quote: ... and their Fc receptors were blocked for 10 min at 4 °C with anti-CD16/CD32 antibodies (10 μg/mL: BD Biosciences). After blocking ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with fluorochrome-conjugated antibodies for 30min at 4°C in the dark in brilliant stain buffer (BD Biosciences). Prior to fixation and permeabilization for intracellular staining with Cytofix/Cytoperm (BD Bioscience) ...
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bioRxiv - Immunology 2020Quote: ... The cells were incubated for one hour at 4°C with a cocktail of fluorochrome-labelled antibodies consisting of anti-CD3-BV510 (cat. no: 563024, BD Biosciences), anti-B220-APC-Cy7 (cat ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested 4 days post-transfection and subjected to fluorescence-activated cell sorting (FACS) for RFP with a FACS Aria III (BD Biosciences). Genomic DNA from sorted RFP-positive cells was extracted using DNeasy Blood & Tissue Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...
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bioRxiv - Microbiology 2021Quote: Spleen and liver cells were incubated (20 min, 4°C) with anti-Fc-gamma blocking antibody (clone 2.4G2) (BD Biosciences, Belgium) and were subsequently stained with fluorescent conjugated antibodies (30 min ...
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bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A and MCF-10A MYC-ER cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). Cells were lysed in Laemmli buffer (50 mM Tris-HCl pH 6.2 ...
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bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). 2D-grown HEK293T ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF-10A and MCF-10A MYC-ER cells were seeded at a density of 10,000 cells per well in triplicate on a 4-well glass chamber slide coated with Matrigel Growth Factor Reduced (BD Biosciences) as described (Debnath et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated during 30 minutes at 4°C with the following primary antibodies: CD11b (clone M1/70, AF647, #557686, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated with the following primary antibodies during 30 minutes at 4°C: CD11b (clone M1/70, APC-Cy7, #557657, BD Pharmingen), CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2022Quote: ... whereas 100 µl PBS+/+ containing 20 ng/ml recombinant IL-1β supplemented with 100 µg/ml PTX3 and/or 100 µg/ml mouse anti-human CD62P (clone AK-4, BD Bioscience) was added to the apical side (upper chamber) ...
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bioRxiv - Immunology 2022Quote: ... single cell suspensions were fixed with CytoFix/CytoPerm for 30 minutes at 4°C followed by permeabilization with BD CytoPerm Plus Buffer (BD Biosciences) for 15 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stored in DPBS with 2% FBS overnight at 4°C before data acquisition on a LSR6Fortessa analyser (BD Biosciences). Compensation was carried out using single stain control UltraComp eBeads (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Colonic LP samples (2 million cell/staining) were stained at 4°C for 20 min with the following antibodies: Ly6C-FITC (AL-21, BD Pharmingen), F4/80-PE (BM8 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C and secondary antibodies for 30 min in RT in Perm/Wash buffer (554714, BD Biosciences) containing 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 4% heat-inactivated FCS containing FACS buffer and data were acquired by a Symphony Flow Cytometer (BD Biosciences). Flow cytometry and t-SNE analyses were performed with FlowJo software (FlowJo10.5.3) ...
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bioRxiv - Immunology 2020Quote: ... IL-4 and IL-10 were quantified using the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine kit (BD Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained overnight at 4°C in 1X perm/wash buffer with FITC mouse Ki67 set (BD Pharmingen #556026). Cells were then washed with 1X perm wash buffer and re-suspended in PBS supplemented with 1ug/ml 4′,6-diamidino-2-phenylindole
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bioRxiv - Genomics 2020Quote: ... Cells were then incubated with 5ul human Fc Block with 95ul 2% FBS in PBS at 4°C for 15 mins before staining with SCMK and AbSeq antibodies (BD Biosciences) at 4°C for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells with normal mCherry fluorescence (mid 70-80% fluorescence) and top or bottom ~7% eGFP fluorescence (~4 million cells/population) were sorted using a BD FACSAria III (BD Biosciences) and collected into 15 ml polypropylene tubes (Cellstar ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 50 nM phorbol-12-myristate-13-acetate (MilliporeSigma) and 250 nM ionomycin (MilliporeSigma) for 4 hours in the presence of Brefeldin A (BD Biosciences) before harvesting ...
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bioRxiv - Immunology 2020Quote: Cells isolated from the organs indicated in figure 4 and figure S9 were incubated with an Fc blocking agent (BD Pharminogen) for 10min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The cells (2-4 × 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibodies to either IFN-γ or IL-4 (BD), and were exposed to various stimuli (e.g ...
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bioRxiv - Microbiology 2020Quote: ... cell debris pelleted by centrifugation (1400 x g for 10 min at 4°C) and supernatants assayed for chemokine and cytokine levels using the CBA flex set (BD Bioscience) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cells were stored overnight at 4°C and were then acquired on a Symphony flow cytometer with Diva software (BD Biosciences). A minimum of 5 × 104 events were acquired for each sample.
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bioRxiv - Immunology 2020Quote: ... stimulation of single cell suspensions in the presence of GolgiPlug for 4 hours prior to intracellular staining using BD Cytofix/Cytoperm kit (BD Biosciences). Viability was determined using Fixable Viability Dye eFluor 780 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... cells were restimulated ex vivo with PMA (50 ng/ml) and ionomycin (750 ng/ml) for 4 h in the presence of GolgiPlug (BD Bioscience), and then fixed and permeabilised following intracellular staining with anti-mouse IL-17A (clone ReBio17B7 ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged and re-suspended in PBS and stored at 4°C prior to cytometric analysis using a using LSRFortessa flow analyzer (BD Biosciences). Results were analyzed using FlowJo V10 software (Flowjo ...
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bioRxiv - Genomics 2021Quote: Healthy human hiPSC cell lines for Figure 4 were cultured under feeder-free conditions on plates coated with human-qualified Matrigel (BD Biosciences) diluted 1:40 in mTeSR-1 medium (StemCell Technologies) ...
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bioRxiv - Immunology 2022Quote: ... The tube was then stored in the dark at 4° C for up to 2 hours prior to acquisition on the LSRII or FACSLyric analyzers (BD Biosciences).
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bioRxiv - Neuroscience 2022Quote: SH-SY5Y cells were seeded at a density of 4×103 cells/well in flat bottom 96-well plates previously coated with Matrigel (BD, Biosciences), and 10 μM all-trans-retinoic acid (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... stained on ice for 30’ with relevant cocktails of antibodies and populations of interest were sorted at 4°C on an BD FACSARIA (BD Bioscience) using a 70 μM nozzle in 50% FBS RPMI ...
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bioRxiv - Immunology 2022Quote: ... intracellular cytokine staining was performed at 4°C for 30 minutes using a cocktail of antibodies resuspended in 1x Perm/Wash buffer (BD Biosciences); intracellular antibodies included IFNγ APC-Cy7 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... cells were detached using 150 μl of PBS + 4 mM EDTA and analyzed via flow cytometry on a BD LSRFortessa Cell Analyzer (BD Biosciences). Transgene expression was detected in the PacBlue channel.
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bioRxiv - Immunology 2022Quote: ... 20 μm paraformaldehyde fixed lung sections from Mtb-mCherry infected I-Tomcat Ai6 and Sp140−/− I-Tomcat Ai6 mice were stained at 4°C overnight with BV421-labeled SIRP□ (P84, BD Biosciences), Pacific Blue–labeled B220 (RA3-6B2 ...
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bioRxiv - Biochemistry 2022Quote: ... Salmonella from infected macrophages was fixed for 15 min in 4% PFA/PBS before analysis on a flow cytometer (Symphony, BD Biosciences) using thresholds on side scatter (SSC ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometry evaluation was performed the following day using a 4-laser (405 nm, 488 nm, 561 nm, 640 nm) LSRFortessa analyzer (BD instruments). The following antibodies and reagents were used for flow cytometry experiments ...
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bioRxiv - Immunology 2022Quote: ... Cells were then resuspended in FACS buffer (1% BSA in D-PBS) and kept at 4°C in dark until analyzed with a customized BD LSR Fortessa flow cytometer (BD Biosciences). Compensation was performed using ArC and UltraComp compensation beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood chimerism was determined in recipients by blood collection via cheek bleeds every 4 weeks for 16 weeks and cells were analyzed by flow cytometry using the LSRII (BD Biosciences). Long-term multi-lineage reconstitution (LTMR ...
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bioRxiv - Pathology 2023Quote: ... then incubated for 20 minutes at 4°C with a combination of the following mouse anti-human antibodies: AF488 anti-CD3 (clone UCHT1, BD Biosciences), PE anti-CD203c (clone NP4D6 ...
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bioRxiv - Cell Biology 2023Quote: ... and cells fixed in 4% paraformaldehyde (PFA) were analyzed on a Stratedigm (S1000EXI) cytometer with post- processing using FlowJo software (BD Biosciences). For all other analyses ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: Lymph node single cell suspension of 2.5×106 cells was incubated for 20 min at 4°C with a purified rat IgG2b anti-mouse CD16/CD32 receptor antibody (BD biosciences). Cells were stained with fluorochrome-conjugated antibodies against CD9 ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Immunology 2023Quote: T cells were staining 30 min at 4°C with anti-human CD3-PE (Cyto Matin Gene, CMGCD3-P100) anti-CD3-PerCP (BD, 347344), anti-CD3-FITC (BD ...
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bioRxiv - Immunology 2023Quote: ... for 20 mins at RT and then fixed samples were then resuspended in 100µL wash buffer prior to acquisition on the 4-laser BD® LSRII (BD Biosciences). Instrument setup and calibration were performed using EuroFlow standard operation procedures as described previously.43
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for 15 min at 4 °C with a purified mouse anti-rat CD32 FcγII (clone D34-485, Rat BD Fc Block) to block unspecific binding ...